LOCUS       RC235079                7755 bp    DNA     circular  SYN 
DEFINITION  LEPR (Myc-DDK tagged) - Homo sapiens leptin receptor (LEPR), transcript variant 4
ACCESSION   NM_001198687
VERSION     NM_001198687.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3912..4003 
                     /label="Myc-DDK"  
     misc_feature    4033..4618 
                     /label="PolyA signal"  
     misc_feature    4090..4109 
                     /label="Restriction Enzyme"  
     misc_feature    4894..5566 
                     /label="ColE1"  
     misc_feature    5996..6790 
                     /label="Kan/Neo"  
     misc_feature    6825..7127 
                     /label="SV40 ori"  
     misc_feature    7213..7690 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3894..3900 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3894 
                     /label="LEPR"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATTTGTCAA AAATTCTGTG TGGTTTTGTT ACATTGGGAA TTTATTTATG 
     1081 TGATAACTGC GTTTAACTTG TCATATCCAA TTACTCCTTG GAGATTTAAG TTGTCTTGCA 
     1141 TGCCACCAAA TTCAACCTAT GACTACTTCC TTTTGCCTGC TGGACTCTCA AAGAATACTT 
     1201 CAAATTCGAA TGGACATTAT GAGACAGCTG TTGAACCTAA GTTTAATTCA AGTGGTACTC 
     1261 ACTTTTCTAA CTTATCCAAA ACAACTTTCC ACTGTTGCTT TCGGAGTGAG CAAGATAGAA 
     1321 ACTGCTCCTT ATGTGCAGAC AACATTGAAG GAAAGACATT TGTTTCAACA GTAAATTCTT 
     1381 TAGTTTTTCA ACAAATAGAT GCAAACTGGA ACATACAGTG CTGGCTAAAA GGAGACTTAA 
     1441 AATTATTCAT CTGTTATGTG GAGTCATTAT TTAAGAATCT ATTCAGGAAT TATAACTATA 
     1501 AGGTCCATCT TTTATATGTT CTGCCTGAAG TGTTAGAAGA TTCACCTCTG GTTCCCCAAA 
     1561 AAGGCAGTTT TCAGATGGTT CACTGCAATT GCAGTGTTCA TGAATGTTGT GAATGTCTTG 
     1621 TGCCTGTGCC AACAGCCAAA CTCAACGACA CTCTCCTTAT GTGTTTGAAA ATCACATCTG 
     1681 GTGGAGTAAT TTTCCAGTCA CCTCTAATGT CAGTTCAGCC CATAAATATG GTGAAGCCTG 
     1741 ATCCACCATT AGGTTTGCAT ATGGAAATCA CAGATGATGG TAATTTAAAG ATTTCTTGGT 
     1801 CCAGCCCACC ATTGGTACCA TTTCCACTTC AATATCAAGT GAAATATTCA GAGAATTCTA 
     1861 CAACAGTTAT CAGAGAAGCT GACAAGATTG TCTCAGCTAC ATCCCTGCTA GTAGACAGTA 
     1921 TACTTCCTGG GTCTTCGTAT GAGGTTCAGG TGAGGGGCAA GAGACTGGAT GGCCCAGGAA 
     1981 TCTGGAGTGA CTGGAGTACT CCTCGTGTCT TTACCACACA AGATGTCATA TACTTTCCAC 
     2041 CTAAAATTCT GACAAGTGTT GGGTCTAATG TTTCTTTTCA CTGCATCTAT AAGAAGGAAA 
     2101 ACAAGATTGT TCCCTCAAAA GAGATTGTTT GGTGGATGAA TTTAGCTGAG AAAATTCCTC 
     2161 AAAGCCAGTA TGATGTTGTG AGTGATCATG TTAGCAAAGT TACTTTTTTC AATCTGAATG 
     2221 AAACCAAACC TCGAGGAAAG TTTACCTATG ATGCAGTGTA CTGCTGCAAT GAACATGAAT 
     2281 GCCATCATCG CTATGCTGAA TTATATGTGA TTGATGTCAA TATCAATATC TCATGTGAAA 
     2341 CTGATGGGTA CTTAACTAAA ATGACTTGCA GATGGTCAAC CAGTACAATC CAGTCACTTG 
     2401 CGGAAAGCAC TTTGCAATTG AGGTATCATA GGAGCAGCCT TTACTGTTCT GATATTCCAT 
     2461 CTATTCATCC CATATCTGAG CCCAAAGATT GCTATTTGCA GAGTGATGGT TTTTATGAAT 
     2521 GCATTTTCCA GCCAATCTTC CTATTATCTG GCTACACAAT GTGGATTAGG ATCAATCACT 
     2581 CTCTAGGTTC ACTTGACTCT CCACCAACAT GTGTCCTTCC TGATTCTGTG GTGAAGCCAC 
     2641 TGCCTCCATC CAGTGTGAAA GCAGAAATTA CTATAAACAT TGGATTATTG AAAATATCTT 
     2701 GGGAAAAGCC AGTCTTTCCA GAGAATAACC TTCAATTCCA GATTCGCTAT GGTTTAAGTG 
     2761 GAAAAGAAGT ACAATGGAAG ATGTATGAGG TTTATGATGC AAAATCAAAA TCTGTCAGTC 
     2821 TCCCAGTTCC AGACTTGTGT GCAGTCTATG CTGTTCAGGT GCGCTGTAAG AGGCTAGATG 
     2881 GACTGGGATA TTGGAGTAAT TGGAGCAATC CAGCCTACAC AGTTGTCATG GATATAAAAG 
     2941 TTCCTATGAG AGGACCTGAA TTTTGGAGAA TAATTAATGG AGATACTATG AAAAAGGAGA 
     3001 AAAATGTCAC TTTACTTTGG AAGCCCCTGA TGAAAAATGA CTCATTGTGC AGTGTTCAGA 
     3061 GATATGTGAT AAACCATCAT ACTTCCTGCA ATGGAACATG GTCAGAAGAT GTGGGAAATC 
     3121 ACACGAAATT CACTTTCCTG TGGACAGAGC AAGCACATAC TGTTACGGTT CTGGCCATCA 
     3181 ATTCAATTGG TGCTTCTGTT GCAAATTTTA ATTTAACCTT TTCATGGCCT ATGAGCAAAG 
     3241 TAAATATCGT GCAGTCACTC AGTGCTTATC CTTTAAACAG CAGTTGTGTG ATTGTTTCCT 
     3301 GGATACTATC ACCCAGTGAT TACAAGCTAA TGTATTTTAT TATTGAGTGG AAAAATCTTA 
     3361 ATGAAGATGG TGAAATAAAA TGGCTTAGAA TCTCTTCATC TGTTAAGAAG TATTATATCC 
     3421 ATGATCATTT TATCCCCATT GAGAAGTACC AGTTCAGTCT TTACCCAATA TTTATGGAAG 
     3481 GAGTGGGAAA ACCAAAGATA ATTAATAGTT TCACTCAAGA TGATATTGAA AAACACCAGA 
     3541 GTGATGCAGG TTTATATGTA ATTGTGCCAG TAATTATTTC CTCTTCCATC TTATTGCTTG 
     3601 GAACATTATT AATATCACAC CAAAGAATGA AAAAGCTATT TTGGGAAGAT GTTCCGAACC 
     3661 CCAAGAATTG TTCCTGGGCA CAAGGACTTA ATTTTCAGAA GATGCTTGAA GGCAGCATGT 
     3721 TCGTTAAGAG TCATCACCAC TCCCTAATCT CAAGTACCCA GGGACACAAA CACTGCGGAA 
     3781 GGCCACAGGG TCCTCTGCAT AGGAAAACCA GAGACCTTTG TTCACTTGTT TATCTGCTGA 
     3841 CCCTCCCTCC ACTATTGTCC TATGACCCTG CCAAATCCCC CTCTGTGAGA AACACCCAAG 
     3901 AAACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     3961 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4021 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4081 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4141 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4201 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4261 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4321 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4381 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4441 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4501 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4561 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     4621 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     4681 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     4741 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     4801 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     4861 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     4921 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     4981 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5041 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5101 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5161 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5221 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5281 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5341 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5401 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     5461 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     5521 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     5581 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     5641 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     5701 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     5761 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     5821 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     5881 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     5941 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6001 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6061 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6121 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6181 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6241 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6301 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6361 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6421 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6481 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     6541 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     6601 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     6661 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     6721 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     6781 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     6841 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     6901 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     6961 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7021 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7081 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7141 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7201 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7261 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7321 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7381 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7441 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7501 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     7561 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     7621 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     7681 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     7741 TTTAACGCGA ATTTT
//