LOCUS       RC235068                7713 bp    DNA     circular  SYN 
DEFINITION  ACTN3 (Myc-DDK tagged) - Homo sapiens actinin, alpha 3 (ACTN3), transcript variant 2
ACCESSION   NM_001258371
VERSION     NM_001258371.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3870..3961 
                     /label="Myc-DDK"  
     misc_feature    3991..4576 
                     /label="PolyA signal"  
     misc_feature    4048..4067 
                     /label="Restriction Enzyme"  
     misc_feature    4852..5524 
                     /label="ColE1"  
     misc_feature    5954..6748 
                     /label="Kan/Neo"  
     misc_feature    6783..7085 
                     /label="SV40 ori"  
     misc_feature    7171..7648 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3852..3858 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3852 
                     /label="ACTN3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGATAGGG CTCTGTGTCC CACCAGCGAG GTGCTGGAAG AGGCTCACGA 
     1081 GGAGCTCCAG AGGGCAGCCT CTAGAGCGGC CCGCGGCCAC TATTATTATC GGCCTTTCTA 
     1141 TTGTGGAGAG GAGTAAACAG ACTCAGAGAG GTCCCGTGGA TTTCTCAAGG TCACACAGCG 
     1201 CCCCAGCAGC GGAGCAGGAA TGCAAACCCA GGATCTCCGC CGCGAGATCC ACCCCGGCGC 
     1261 CCCCGAGTCC CGCAGTTCCC GGCTTCAGGA CAACCCCTTG CCAGACCTTC ACTGCCTGGT 
     1321 GCAACTCACA CCTGCGCAAG GCAGGCACCC AGATCGAGAA CATCGAGGAA GATTTCCGCA 
     1381 ATGGCCTCAA ACTCATGCTG CTCCTGGAGG TCATTTCAGG TGAGAGGCTG CCTAGGCCAG 
     1441 ATAAAGGCAA GATGCGCTTC CACAAAATCG CCAACGTTAA CAAGGCCCTG GACTTCATTG 
     1501 CCAGCAAGGG GGTTAAACTG GTGTCCATTG GTGCTGAAGA GATTGTTGAC GGGAACCTGA 
     1561 AGATGACCCT GGGCATGATC TGGACCATCA TCCTTCGCTT CGCCATCCAG GACATCTCTG 
     1621 TGGAAGAAAC CTCAGCCAAG GAAGGCTTGC TTCTGTGGTG CCAGAGGAAG ACAGCACCGT 
     1681 ACCGCAACGT CAACGTGCAG AACTTCCACA CCAGCTGGAA GGATGGCCTG GCCCTCTGTG 
     1741 CCCTCATCCA CCGACACCGC CCTGACCTCA TCGACTACGC CAAACTGCGA AAGGATGACC 
     1801 CCATCGGAAA CCTGAACACT GCCTTTGAGG TGGCAGAGAA ATACCTGGAC ATCCCCAAGA 
     1861 TGTTGGATGC AGAAGACATT GTGAACACCC CAAAGCCGGA TGAGAAGGCC ATCATGACCT 
     1921 ATGTGTCCTG CTTCTACCAT GCCTTTGCCG GGGCTGAGCA GGCAGAGACA GCTGCCAACA 
     1981 GGATCTGCAA GGTGCTGGCA GTGAACCAGG AAAACGAGAA GCTGATGGAG GAGTATGAGA 
     2041 AGCTTGCCAG TGAGCTGCTG GAGTGGATCC GCCGCACTGT CCCATGGCTG GAGAACCGTG 
     2101 TGGGTGAGCC CAGCATGAGT GCCATGCAGC GCAAACTAGA GGACTTTCGG GACTACCGGC 
     2161 GTCTGCACAA GCCGCCCCGC ATTCAGGAAA AGTGCCAGCT GGAGATCAAC TTCAACACAC 
     2221 TGCAGACCAA GTTGCGGCTC AGCCACCGGC CTGCCTTCAT GCCCTCCGAG GGCAAGCTGG 
     2281 TCTCGGACAT CGCCAACGCC TGGCGGGGGC TGGAGCAGGT GGAAAAGGGC TATGAGGACT 
     2341 GGCTGCTCTC GGAGATCCGG CGCCTGCAGC GACTCCAGCA CCTGGCTGAG AAGTTCCGGC 
     2401 AGAAGGCCTC CCTGCACGAA GCCTGGACCC GGGGAAAGGA GGAGATGCTG AGCCAGCGCG 
     2461 ACTACGATTC GGCTTTGCTA CAGGAGGTGC GGGCGTTGCT GCGGCGCCAC GAGGCCTTTG 
     2521 AGAGCGACCT GGCGGCGCAC CAGGACCGCG TGGAGCACAT TGCCGCGCTG GCCCAGGAGC 
     2581 TCAATGAGCT GGACTACCAC GAGGCAGCCT CAGTGAATAG CCGCTGCCAG GCCATCTGCG 
     2641 ATCAGTGGGA CAACCTGGGC ACCCTGACCC AGAAGAGGCG GGATGCGCTA GAGCGGATGG 
     2701 AGAAGCTCCT GGAGACCATT GACCAGCTGC AACTGGAGTT TGCCCGGCGG GCCGCGCCCT 
     2761 TCAACAACTG GCTGGATGGT GCCGTGGAGG ACCTGCAGGA CGTGTGGCTG GTACACTCTG 
     2821 TGGAGGAGAC CCAGAGCCTG CTGACAGCGC ACGATCAGTT CAAGGCAACA CTGCCCGAGG 
     2881 CTGACCGAGA GCGAGGTGCC ATCATGGGCA TCCAGGGTGA GATCCAGAAG ATCTGCCAGA 
     2941 CGTATGGGCT GCGGCCCTGC TCCACCAATC CCTACATCAC CCTCAGCCCG CAGGACATCA 
     3001 ACACCAAGTG GGATATGGTC CGAAAGCTGG TGCCCAGCCG TGACCAGACA CTGCAGGAGG 
     3061 AGCTGGCACG GCAGCAGGTA AACGAGAGGC TCCGGCGACA GTTTGCGGCC CAGGCCAATG 
     3121 CCATTGGACC CTGGATCCAG GCGAAGGTGG AGGAAGTGGG GCGGCTGGCA GCAGGGCTAG 
     3181 CTGGCTCTCT GGAGGAGCAG ATGGCTGGGC TACGGCAGCA GGAGCAGAAC ATTATCAACT 
     3241 ACAAGACTAA CATTGACCGG CTGGAGGGTG ACCACCAGCT GCTGCAGGAG AGCCTGGTGT 
     3301 TCGACAATAA GCACACCGTC TACAGCATGG AGCACATCCG CGTGGGCTGG GAGCAGCTGC 
     3361 TCACCTCCAT TGCCCGCACC ATCAATGAAG TGGAGAACCA GGTACTGACC CGAGACGCCA 
     3421 AGGGACTGAG CCAGGAGCAG CTCAACGAGT TCCGAGCATC CTTCAACCAC TTTGACAGGA 
     3481 AGCGGAATGG GATGATGGAG CCTGATGACT TCCGAGCTTG CCTCATCTCC ATGGGCTATG 
     3541 ACCTGGGGGA AGTGGAGTTT GCTCGCATCA TGACCATGGT GGACCCCAAC GCAGCTGGGG 
     3601 TGGTGACCTT CCAGGCCTTC ATAGACTTCA TGACCCGAGA GACAGCCGAG ACTGACACGA 
     3661 CTGAGCAAGT TGTAGCTTCC TTCAAGATCT TGGCAGGAGA CAAGAACTAC ATCACCCCCG 
     3721 AGGAGCTGCG GCGCGAGCTC CCTGCCAAGC AGGCCGAGTA CTGCATCCGC CGTATGGTGC 
     3781 CCTACAAGGG ATCCGGGGCC CCGGCTGGAG CCCTGGACTA CGTGGCCTTC TCCAGTGCCC 
     3841 TCTATGGGGA GAGCGACCTT ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     3901 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     3961 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4021 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4081 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4141 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4201 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4261 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4321 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4381 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4441 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4501 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4561 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4621 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     4681 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     4741 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     4801 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     4861 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     4921 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     4981 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5041 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5101 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5161 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5221 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5281 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5341 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5401 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5461 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5521 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5581 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     5641 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     5701 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     5761 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     5821 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     5881 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     5941 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6001 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6061 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6121 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6181 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6241 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6301 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6361 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6421 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6481 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6541 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6601 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     6661 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     6721 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     6781 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     6841 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     6901 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     6961 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7021 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7081 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7141 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7201 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7261 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7321 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7381 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7441 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7501 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7561 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7621 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     7681 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//