LOCUS       RC235056                7683 bp    DNA     circular  SYN 
DEFINITION  DGKZ (Myc-DDK tagged) - Homo sapiens diacylglycerol kinase, zeta (DGKZ), transcript variant 5
ACCESSION   NM_001199266
VERSION     NM_001199266.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3840..3931 
                     /label="Myc-DDK"  
     misc_feature    3961..4546 
                     /label="PolyA signal"  
     misc_feature    4018..4037 
                     /label="Restriction Enzyme"  
     misc_feature    4822..5494 
                     /label="ColE1"  
     misc_feature    5924..6718 
                     /label="Kan/Neo"  
     misc_feature    6753..7055 
                     /label="SV40 ori"  
     misc_feature    7141..7618 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3822..3828 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3822 
                     /label="DGKZ"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCCGCGG GACGGTAGCC CCGAGGCCCG GAGCAGCGAC TCCGAGTCGG 
     1081 CTTCCGCCTC GTCCAGCGGC TCCGAGCGCG ACGCCGGTCC CGAGCCGGAC AAGGCGCCGC 
     1141 GGCGACTCAA CAAGCGGCGC TTCCCGGGGC TGCGGCTCTT CGGGCACAGG AAAGCCATCA 
     1201 CCAAGTCGGG CCTCCAGCAC CTGGCCCCCC CTCCGCCCAC CCCTGGGGCC CCGTGCAGCG 
     1261 AGTCAGAGCG GCAGATCCGG AGTACAGTGG ACTGGAGCGA GTCAGCGACA TATGGGGAGC 
     1321 ACATCTGGTT CGAGACCAAC GTGTCCGGGG ACTTCTGCTA CGTTGGGGAG CAGTACTGTG 
     1381 TAGCCAGGAT GCTGCAGAAG TCAGTGTCTC GAAGAAAGTG CGCAGCCTGC AAGATTGTGG 
     1441 TGCACACGCC CTGCATCGAG CAGCTGGAGA AGATAAATTT CCGCTGTAAG CCGTCCTTCC 
     1501 GTGAATCAGG CTCCAGGAAT GTCCGCGAGC CAACCTTTGT ACGGCACCAC TGGGTACACA 
     1561 GACGACGCCA GGACGGCAAG TGTCGGCACT GTGGGAAGGG ATTCCAGCAG AAGTTCACCT 
     1621 TCCACAGCAA GGAGATTGTG GCCATCAGCT GCTCGTGGTG CAAGCAGGCA TACCACAGCA 
     1681 AGGTGTCCTG CTTCATGCTG CAGCAGATCG AGGAGCCGTG CTCGCTGGGG GTCCACGCAG 
     1741 CCGTGGTCAT CCCGCCCACC TGGATCCTCC GCGCCCGGAG GCCCCAGAAT ACTCTGAAAG 
     1801 CAAGCAAGAA GAAGAAGAGG GCATCCTTCA AGAGGAAGTC CAGCAAGAAA GGGCCTGAGG 
     1861 AGGGCCGCTG GAGACCCTTC ATCATCAGGC CCACCCCCTC CCCGCTCATG AAGCCCCTGC 
     1921 TGGTGTTTGT GAACCCCAAG AGTGGGGGCA ACCAGGGTGC AAAGATCATC CAGTCTTTCC 
     1981 TCTGGTATCT CAATCCCCGA CAAGTCTTCG ACCTGAGCCA GGGAGGGCCC AAGGAGGCGC 
     2041 TGGAGATGTA CCGCAAAGTG CACAACCTGC GGATCCTGGC GTGCGGGGGC GACGGCACGG 
     2101 TGGGCTGGAT CCTCTCCACC CTGGACCAGC TACGCCTGAA GCCGCCACCC CCTGTTGCCA 
     2161 TCCTGCCCCT GGGTACTGGC AACGACTTGG CCCGAACCCT CAACTGGGGT GGGGGCTACA 
     2221 CAGATGAGCC TGTGTCCAAG ATCCTCTCCC ACGTGGAGGA GGGGAACGTG GTACAGCTGG 
     2281 ACCGCTGGGA CCTCCACGCT GAGCCCAACC CCGAGGCAGG GCCTGAGGAC CGAGATGAAG 
     2341 GCGCCACCGA CCGGTTGCCC CTGGATGTCT TCAACAACTA CTTCAGCCTG GGCTTTGACG 
     2401 CCCACGTCAC CCTGGAGTTC CACGAGTCTC GAGAGGCCAA CCCAGAGAAA TTCAACAGCC 
     2461 GCTTTCGGAA TAAGATGTTC TACGCCGGGA CAGCTTTCTC TGACTTCCTG ATGGGCAGCT 
     2521 CCAAGGACCT GGCCAAGCAC ATCCGAGTGG TGTGTGATGG AATGGACTTG ACTCCCAAGA 
     2581 TCCAGGACCT GAAACCCCAG TGTGTTGTTT TCCTGAACAT CCCCAGGTAC TGTGCGGGCA 
     2641 CCATGCCCTG GGGCCACCCT GGGGAGCACC ACGACTTTGA GCCCCAGCGG CATGACGACG 
     2701 GCTACCTCGA GGTCATTGGC TTCACCATGA CGTCGTTGGC CGCGCTGCAG GTGGGCGGAC 
     2761 ACGGCGAGCG GCTGACGCAG TGTCGCGAGG TGGTGCTCAC CACATCCAAG GCCATCCCGG 
     2821 TGCAGGTGGA TGGCGAGCCC TGCAAGCTTG CAGCCTCACG CATCCGCATC GCCCTGCGCA 
     2881 ACCAGGCCAC CATGGTGCAG AAGGCCAAGC GGCGGAGCGC CGCCCCCCTG CACAGCGACC 
     2941 AGCAGCCGGT GCCAGAGCAG TTGCGCATCC AGGTGAGTCG CGTCAGCATG CACGACTATG 
     3001 AGGCCCTGCA CTACGACAAG GAGCAGCTCA AGGAGGCCTC TGTGCCGCTG GGCACTGTGG 
     3061 TGGTCCCAGG AGACAGTGAC CTAGAGCTCT GCCGTGCCCA CATTGAGAGA CTCCAGCAGG 
     3121 AGCCCGATGG TGCTGGAGCC AAGTCCCCGA CATGCCAGAA ACTGTCCCCC AAGTGGTGCT 
     3181 TCCTGGACGC CACCACTGCC AGCCGCTTCT ACAGGATCGA CCGAGCCCAG GAGCACCTCA 
     3241 ACTATGTGAC TGAGATCGCA CAGGATGAGA TTTATATCCT GGACCCTGAG CTGCTGGGGG 
     3301 CATCGGCCCG GCCTGACCTC CCAACCCCCA CTTCCCCTCT CCCCACCTCA CCCTGCTCAC 
     3361 CCACGCCCCG GTCACTGCAA GGGGATGCTG CACCCCCTCA AGGTGAAGAG CTGATTGAGG 
     3421 CTGCCAAGAG GAACGACTTC TGTAAGCTCC AGGAGCTGCA CCGAGCTGGG GGCGACCTCA 
     3481 TGCACCGAGA CGAGCAGAGT CGCACGCTCC TGCACCACGC AGTCAGCACT GGCAGCAAGG 
     3541 ATGTGGTCCG CTACCTGCTG GACCACGCCC CCCCAGAGAT CCTTGATGCG GTGGAGGAAA 
     3601 ACCCCTGCTC CCCCAGCGGG GAGACCTGTT TGCACCAAGC AGCGGCCCTG GGCCAGCGCA 
     3661 CCATCTGCCA CTACATCGTG GAGGCCGGGG CCTCGCTCAT GAAGACAGAC CAGCAGGGCG 
     3721 ACACTCCCCG GCAGCGGGCT GAGAAGGCTC AGGACACCGA GCTGGCCGCC TACCTGGAGA 
     3781 ACCGGCAGCA CTACCAGATG ATCCAGCGGG AGGACCAGGA GACGGCTGTG ACGCGTACGC 
     3841 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     3901 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     3961 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4021 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4081 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4141 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4201 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4261 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4321 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4381 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4441 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4501 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4561 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     4621 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     4681 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     4741 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     4801 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     4861 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     4921 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     4981 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5041 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5101 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5161 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5221 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5281 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5341 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     5401 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     5461 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     5521 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     5581 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     5641 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     5701 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     5761 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     5821 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     5881 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     5941 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6001 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6061 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6121 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6181 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6241 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6301 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6361 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     6421 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     6481 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     6541 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     6601 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     6661 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     6721 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     6781 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     6841 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     6901 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     6961 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7021 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7081 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7141 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7201 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7261 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7321 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7381 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     7441 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     7501 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     7561 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     7621 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     7681 TTT
//