LOCUS       RC235038                7641 bp    DNA     circular  SYN 
DEFINITION  RTN3 (Myc-DDK tagged) - Homo sapiens reticulon 3 (RTN3), transcript variant 6
ACCESSION   NM_001265590
VERSION     NM_001265590.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3798..3889 
                     /label="Myc-DDK"  
     misc_feature    3919..4504 
                     /label="PolyA signal"  
     misc_feature    3976..3995 
                     /label="Restriction Enzyme"  
     misc_feature    4780..5452 
                     /label="ColE1"  
     misc_feature    5882..6676 
                     /label="Kan/Neo"  
     misc_feature    6711..7013 
                     /label="SV40 ori"  
     misc_feature    7099..7576 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3780..3786 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3780 
                     /label="RTN3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGAGCCG TCGGCGGCCA CTCAGTCCCA TTCCATCTCC TCGTCGTCCT 
     1081 TCGGAGCCGA GCCGTCCGCG CCCGGCGGCG GCGGGAGCCC AGGAGCCTGC CCCGCCCTGG 
     1141 GGACGAAGAG CTGCAGCTCC TCCTGTGCGG CCAGTTTCCC AGAGCATCCT GCTTTTCTCT 
     1201 CAAAGAAAAT TGGTCAAGTG GAAGAGCAAA TAGATAAAGA GACCAAGAAC CCAAATGGGG 
     1261 TATCAAGTAG GGAGGCTAAA ACTGCATTGG ATGCTGATGA CAGATTCACT TTGCTGACAG 
     1321 CCCAGAAACC ACCTACTGAG TACTCTAAGG TAGAAGGCAT TTATACATAT TCTTTGTCTC 
     1381 CATCCAAAGT TTCAGGAGAT GATGTTATTG AAAAGGATTC CCCTGAATCA CCATTTGAAG 
     1441 TAATTATTGA CAAAGCAGCA TTTGACAAAG AATTTAAAGA CTCATATAAG GAGAGCACAG 
     1501 ATGATTTTGG TAGCTGGTCT GTGCACACTG ATAAAGAATC ATCCGAAGAC ATTTCAGAGA 
     1561 CTAATGACAA GCTTTTTCCA CTGAGAAATA AAGAGGCAGG ACGTTACCCA ATGTCTGCAT 
     1621 TGCTCAGTAG GCAGTTTTCA CACACAAATG CAGCACTGGA AGAGGTGTCC AGATGCGTGA 
     1681 ATGATATGCA TAACTTTACT AACGAAATAC TGACTTGGGA TCTGGTTCCC CAAGTGAAAC 
     1741 AACAGACCGA TAAATCTTCT GACTGCATCA CAAAAACTAC AGGACTTGAC ATGAGTGAAT 
     1801 ATAATTCAGA AATTCCAGTT GTAAATCTTA AAACTAGCAC TCATCAGAAA ACTCCTGTAT 
     1861 GTTCTATTGA TGGGAGCACT CCCATCACTA AATCAACAGG TGATTGGGCA GAAGCATCTC 
     1921 TCCAGCAAGA AAATGCTATT ACTGGAAAAC CTGTACCTGA CTCTTTGAAT TCCACAAAAG 
     1981 AATTCAGTAT CAAAGGTGTG CAAGGCAATA TGCAGAAACA GGATGACACA CTTGCAGAAT 
     2041 TACCTGGATC TCCACCTGAG AAATGTGACT CTTTGGGTTC TGGAGTGGCC ACAGTGAAAG 
     2101 TGGTTTTACC TGATGACCAC CTGAAAGATG AAATGGACTG GCAGAGCTCT GCATTGGGAG 
     2161 AAATCACAGA AGCTGATAGT TCTGGTGAGT CTGATGACAC AGTAATAGAG GACATCACAG 
     2221 CAGATACATC ATTTGAAAAT AACAAAATTC AGGCTGAAAA ACCTGTTTCC ATTCCAAGTG 
     2281 CTGTTGTAAA AACAGGTGAA AGAGAAATCA AAGAGATTCC CAGTTGTGAG AGAGAAGAAA 
     2341 AAACATCTAA AAACTTTGAA GAATTGGTCA GTGACTCTGA GCTGCATCAA GATCAGCCTG 
     2401 ATATTCTTGG AAGGAGTCCA GCTAGTGAGG CAGCATGTTC AAAAGTACCC GATACGAATG 
     2461 TCTCCTTAGA AGATGTGAGT GAAGTTGCTC CTGAAAAGCC TATTACTACT GAGAACCCCA 
     2521 AACTTCCTTC AACAGTGTCT CCAAATGTTT TTAATGAGAC AGAATTCTCA TTAAATGTGA 
     2581 CAACATCTGC CTATTTGGAG TCATTACATG GGAAAAATGT TAAACATATA GATGATTCCT 
     2641 CCCCAGAGGA CCTGATAGCA GCCTTTACAG AAACCAGAGA TAAAGGAATA GTAGATAGTG 
     2701 AAAGAAATGC TTTTAAAGCA ATATCAGAGA AGATGACAGA CTTTAAAACA ACTCCTCCTG 
     2761 TAGAAGTCTT ACATGAAAAT GAGTCCGGTG GTTCTGAAAT TAAAGACATT GGAAGCAAAT 
     2821 ACAGTGAACA AAGCAAAGAA ACAAATGGAA GTGAGCCTCT AGGTGTTTTC CCTACCCAAG 
     2881 GTACTCCAGT AGCATCTCTT GACTTAGAAC AAGAACAGCT CACAATTAAG GCTCTTAAAG 
     2941 AATTAGGTGA AAGACAGGTT GAGAAGTCAA CTTCTGCACA GCGTGACGCA GAATTGCCTT 
     3001 CTGAAGAAGT ACTGAAGCAA ACTTTCACAT TTGCTCCAGA ATCTTGGCCA CAGAGATCAT 
     3061 ATGACATCCT AGAACGTAAT GTCAAGAATG GATCTGATCT TGGGATTTCC CAGAAGCCCA 
     3121 TCACTATCAG AGAAACTACT AGGGTAGATG CTGTTTCCAG CCTTAGCAAG ACTGAATTGG 
     3181 TAAAAAAGCA TGTCCTAGCA AGACTTCTGA CAGACTTCTC AGTGCACGAT CTGATTTTCT 
     3241 GGAGAGATGT GAAGAAGACT GGGTTTGTCT TTGGCACCAC GCTGATCATG CTGCTTTCCC 
     3301 TGGCAGCTTT CAGTGTCATC AGTGTGGTTT CTTACCTCAT CCTGGCTCTT CTCTCTGTCA 
     3361 CCATCAGCTT CAGGATCTAC AAGTCCGTCA TCCAAGCTGT ACAGAAGTCA GAAGAAGGCC 
     3421 ATCCATTCAA AGCCTACCTG GACGTAGACA TTACTCTGTC CTCAGAAGCT TTCCATAATT 
     3481 ACATGAATGC TGCCATGGTG CACATCAACA GGGCCCTGAA ACTCATTATT CGTCTCTTTC 
     3541 TGGTAGAAGA TCTGGTTGAC TCCTTGAAGC TGGCTGTCTT CATGTGGCTG ATGACCTATG 
     3601 TTGGTGCTGT TTTTAACGGA ATCACCCTTC TAATTCTTGC TGAACTGCTC ATTTTCAGTG 
     3661 TCCCGATTGT CTATGAGAAG TACAAGACCC AGATTGATCA CTATGTTGGC ATCGCCCGAG 
     3721 ATCAGACCAA GTCAATTGTT GAAAAGATCC AAGCAAAACT CCCTGGAATC GCCAAAAAAA 
     3781 AGGCAGAAAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     3841 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     3901 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     3961 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4021 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4081 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4141 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4201 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4261 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4321 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4381 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4441 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     4501 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     4561 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     4621 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     4681 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     4741 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     4801 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     4861 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     4921 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     4981 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5041 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5101 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5161 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5221 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     5281 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     5341 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     5401 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     5461 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     5521 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     5581 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     5641 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     5701 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     5761 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     5821 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     5881 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     5941 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6001 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6061 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6121 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6181 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6241 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6301 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     6361 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     6421 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     6481 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     6541 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     6601 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     6661 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     6721 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     6781 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     6841 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     6901 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6961 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7021 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7081 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7141 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7201 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7261 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7321 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     7381 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     7441 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     7501 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     7561 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     7621 CAAAAATTTA ACGCGAATTT T
//