LOCUS       RC235034                7647 bp    DNA     circular  SYN 
DEFINITION  TJP3 (Myc-DDK tagged) - Homo sapiens tight junction protein 3 (TJP3), transcript variant 1
ACCESSION   NM_001267560
VERSION     NM_001267560.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3802..3893 
                     /label="Myc-DDK"  
     misc_feature    3923..4508 
                     /label="PolyA signal"  
     misc_feature    3980..3999 
                     /label="Restriction Enzyme"  
     misc_feature    4784..5456 
                     /label="ColE1"  
     misc_feature    5886..6680 
                     /label="Kan/Neo"  
     misc_feature    6715..7017 
                     /label="SV40 ori"  
     misc_feature    7103..7580 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3777..3784 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3777 
                     /label="TJP3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGAGCTG ACCATCTGGG AACAGCACAC GGCCACACTG TCCAAGGACC 
     1081 CCCGCCGGGG CTTTGGCATT GCGATCTCTG GAGGCCGAGA CCGGCCCGGT GGATCCATGG 
     1141 TTGTATCTGA CGTGGTACCT GGAGGGCCGG CGGAGGGCAG GCTACAGACA GGCGACCACA 
     1201 TCGTCATGGT GAACGGGGTT TCCATGGAGA ATGCCACCTC CGCGTTTGCC ATTCAGATAC 
     1261 TCAAGACCTG CACCAAGATG GCCAACATCA CAGTGAAACG TCCCCGGAGG ATCCACCTGC 
     1321 CCGCCACCAA AGCCAGCCCC TCCAGCCCAG GGCGCCAGGA CTCGGATGAA GACGATGGGC 
     1381 CCCAGCGGGT GGAGGAGGTG GACCAGGGCC GGGGCTATGA CGGCGACTCA TCCAGTGGCT 
     1441 CCGGCCGCTC CTGGGACGAG CGCTCCCGCC GGCCGAGGCC TGGTCGCCGG GGCCGGGCCG 
     1501 GCAGCCATGG GCGTAGGAGC CCAGGTGGTG GCTCTGAGGC CAACGGGCTG GCCCTGGTGT 
     1561 CCGGCTTTAA GCGGCTGCCA CGGCAGGACG TGCAGATGAA GCCTGTGAAG TCAGTGCTGG 
     1621 TGAAGAGGAG AGACAGCGAA GAGTTTGGCG TCAAGCTGGG CAGTCAGATC TTCATCAAGC 
     1681 ACATTACAGA TTCGGGCCTG GCTGCCCGGC ACCGTGGGCT GCAGGAAGGA GATCTCATTC 
     1741 TACAGATCAA CGGGGTGTCT AGCCAGAACC TGTCACTGAA CGACACCCGG CGACTGATTG 
     1801 AGAAGTCAGA AGGGAAGCTA AGCCTGCTGG TGCTGAGAGA TCGTGGGCAG TTCCTGGTGA 
     1861 ACATTCCGCC TGCTGTCAGT GACAGCGACA GCTCGCCATT GGAGGACATC TCGGACCTCG 
     1921 CCTCGGAGCT ATCGCAGGCA CCACCATCCC ACATCCCACC ACCACCCCGG CATGCTCAGC 
     1981 GGAGCCCCGA GGCCAGCCAG ACCGACTCTC CCGTGGAGAG TCCCCGGCTT CGGCGGGAAA 
     2041 GTTCAGTAGA TTCCAGAACC ATCTCGGAAC CAGATGAGCA ACGGTCAGAG TTGCCCAGGG 
     2101 AAAGCAGCTA TGACATCTAC AGAGTGCCCA GCAGTCAGAG CATGGAGGAT CGTGGGTACA 
     2161 GCCCCGACAC GCGTGTGGTC CGCTTCCTCA AGGGCAAGAG CATCGGGCTG CGGCTGGCAG 
     2221 GGGGCAATGA CGTGGGCATC TTCGTGTCCG GGGTGCAGGC GGGCAGCCCG GCCGACGGGC 
     2281 AGGGCATCCA GGAGGGAGAT CAGATTCTGC AGGTGAATGA CGTGCCATTC CAGAACCTGA 
     2341 CACGGGAGGA GGCAGTGCAG TTCCTGCTGG GGCTGCCACC AGGCGAGGAG ATGGAGCTGG 
     2401 TGACGCAGAG GAAGCAGGAC ATTTTCTGGA AAATGGTGCA GTCCCGCGTG GGTGACTCCT 
     2461 TCTACATCCG CACTCACTTT GAGCTGGAGC CCAGTCCACC GTCTGGCCTG GGCTTCACCC 
     2521 GTGGCGACGT CTTCCACGTG CTGGACACGC TGCACCCCGG CCCCGGGCAG AGCCACGCAC 
     2581 GAGGAGGCCA CTGGCTGGCG GTGCGCATGG GTCGTGACCT GCGGGAGCAA GAGCGGGGCA 
     2641 TCATTCCCAA CCAGAGCAGG GCGGAGCAGC TGGCCAGCCT GGAAGCTGCC CAGAGGGCCG 
     2701 TGGGAGTCGG GCCCGGCTCC TCCGCGGGCT CCAATGCTCG GGCCGAGTTC TGGCGGCTGC 
     2761 GGGGTCTTCG TCGAGGAGCC AAGAAGACCA CTCAGCGGAG CCGTGAGGAC CTCTCAGCTC 
     2821 TGACCCGACA GGGCCGCTAC CCGCCCTACG AACGAGTGGT GTTGCGAGAA GCCAGTTTCA 
     2881 AGCGCCCGGT AGTGATCCTG GGACCCGTGG CCGACATTGC TATGCAGAAG TTGACTGCTG 
     2941 AGATGCCTGA CCAGTTTGAA ATCGCAGAGA CTGTGTCCAG GACCGACAGC CCCTCCAAGA 
     3001 TCATCAAACT AGACACCGTG CGGGTGATTG CAGAAAAAGA CAAGCATGCG CTCCTGGATG 
     3061 TGACCCCCTC CGCCATCGAG CGCCTCAACT ATGTGCAGTA CTACCCCATT GTGGTCTTCT 
     3121 TCATCCCCGA GAGCCGGCCG GCCCTCAAGG CACTGCGCCA GTGGCTGGCG CCTGCCTCCC 
     3181 GCCGCAGCAC CCGTCGCCTC TACGCACAAG CCCAGAAGCT GCGAAAACAC AGCAGCCACC 
     3241 TCTTCACAGC CACCATCCCT CTGAATGGCA CGAGTGACAC CTGGTACCAG GAGCTCAAGG 
     3301 CCATCATTCG AGAGCAGCAG ACGCGGCCCA TCTGGACGGC GGAAGATCAG CTGGATGGCT 
     3361 CCTTGGAGGA CAACCTAGAC CTCCCTCACC ACGGCCTGGC CGACAGCTCC GCTGACCTCA 
     3421 GCTGCGACAG CCGCGTTAAC AGCGACTACG AGACGGACGG CGAGGGCGGC GCGTACACGG 
     3481 ATGGCGAGGG CTACACAGAC GGCGAGGGGG GGCCCTACAC GGATGTGGAT GATGAGCCCC 
     3541 CGGCTCCAGC CCTGGCCCGG TCCTCGGAGC CCGTGCAGGC AGATGAGTCC CAGAGCCCGA 
     3601 GGGATCGTGG GAGAATCTCG GCTCATCAGG GGGCCCAGGT GGACAGCCGC CACCCCCAGG 
     3661 GACAGTGGCG ACAGGACAGC ATGCGAACCT ATGAACGGGA AGCCCTGAAG AAAAAGTTTA 
     3721 TGCGAGTACA TGATGCGGAG TCCTCCGATG AAGACGGCTA TGACTGGGGT CCGGCCACTG 
     3781 ACCTGAGCGG ACCGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     3841 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     3901 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     3961 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4021 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4081 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4141 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4201 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4261 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4321 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4381 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     4441 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     4501 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     4561 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     4621 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     4681 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     4741 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     4801 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     4861 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     4921 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     4981 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5041 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5101 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5161 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     5221 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     5281 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     5341 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     5401 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     5461 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     5521 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     5581 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     5641 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     5701 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     5761 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     5821 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     5881 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     5941 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6001 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6061 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6121 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6181 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6241 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     6301 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     6361 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     6421 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     6481 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     6541 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     6601 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     6661 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     6721 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     6781 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     6841 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     6901 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     6961 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7021 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7081 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7141 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7201 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7261 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     7321 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     7381 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     7441 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     7501 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     7561 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     7621 ATTTAACAAA AATTTAACGC GAATTTT
//