LOCUS       RC235027                7620 bp    DNA     circular  SYN 
DEFINITION  RALGDS (Myc-DDK tagged) - Homo sapiens ral guanine nucleotide dissociation stimulator (RALGDS), transcript variant 4
ACCESSION   NM_001271775
VERSION     NM_001271775.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3777..3868 
                     /label="Myc-DDK"  
     misc_feature    3898..4483 
                     /label="PolyA signal"  
     misc_feature    3955..3974 
                     /label="Restriction Enzyme"  
     misc_feature    4759..5431 
                     /label="ColE1"  
     misc_feature    5861..6655 
                     /label="Kan/Neo"  
     misc_feature    6690..6992 
                     /label="SV40 ori"  
     misc_feature    7078..7555 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3759..3765 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3759 
                     /label="RALGDS"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCGGGAA GCAGGCCAGG TTTGTGCCAG GCCTGCCGTG CCCAGGGGGA 
     1081 GGAAGGGCTC TGTGTTCTTT GCCTGTGTCT CTGTGGTGAC CGCCAGGAGA AGGGCCGTCG 
     1141 CCCGTCGTGC CGCTCTCCAA AGCCCGACAC CTTGGCTGGC ACCTCTGCCA GCACCGGCCA 
     1201 CCACCGAGAG CTCCACGCAG GAGATCGGTG AGGAGCTGAT CAACGGAGTC ATCTACTCCA 
     1261 TCTCCCTGCG CAAGGTGCAG CTGCACCACG GAGGCAACAA GGGGCAGCGC TGGCTCGGGT 
     1321 ATGAGAATGA GTCGGCCCTG AACCTTTATG AGACTTGCAA GGTGCGGACC GTGAAGGCTG 
     1381 GCACGCTGGA GAAGCTGGTG GAGCACCTGG TGCCAGCCTT CCAGGGCAGC GACCTCTCCT 
     1441 ACGTCACCAT CTTCCTGTGT ACCTATAGAG CCTTCACCAC CACCCAACAG GTCCTGGACC 
     1501 TGCTGTTCAA AAGGTACGGT AGATGTGACG CCCTCACGGC CTCCTCTAGA TACGGCTGCA 
     1561 TCCTCCCCTA TTCCGACGAG GATGGTGGAC CCCAGGACCA ACTTAAAAAT GCCATCTCCT 
     1621 CCATCCTGGG CACCTGGCTG GACCAGTACT CGGAGGATTT CTGTCAACCT CCGGACTTTC 
     1681 CCTGCCTCAA GCAGCTGGTG GCCTACGTGC AGCTCAACAT GCCAGGCTCA GACCTGGAGC 
     1741 GCCGTGCCCA CCTTCTCCTG GCCCAGCTGG AGCACTCGGA ACCCATTGAG GCAGAGCCTG 
     1801 AGGCTCTGTC ACCAGTGCCA GCTCTAAAAC CAACTCCAGA GCTCGAGCTA GCTCTAACAC 
     1861 CAGCTCGAGC ACCCAGCCCA GTGCCGGCTC CAGCCCCGGA GCCAGAGCCA GCTCCAACAC 
     1921 CAGCTCCAGG TTCAGAGCTA GAAGTAGCTC CAGCACCAGC TCCGGAGCTC CAGCAGGCTC 
     1981 CAGAGCCAGC TGTGGGACTA GAATCGGCTC CAGCGCCAGC TCTGGAACTA GAGCCAGCTC 
     2041 CAGAACAGGA TCCAGCTCCC TCACAAACTC TAGAGCTGGA GCCAGCTCCA GCACCAGTTC 
     2101 CATCATTACA GCCTTCCTGG CCTTCACCTG TGGTTGCAGA GAACGGGCTG AGTGAGGAGA 
     2161 AGCCTCACCT CTTGGTGTTC CCTCCAGATC TGGTGGCAGA GCAGTTTACA CTGATGGATG 
     2221 CGGAACTGTT CAAGAAGGTG GTGCCCTACC ACTGCCTGGG CTCCATCTGG TCCCAGCGGG 
     2281 ACAAGAAGGG CAAGGAGCAC CTGGCGCCCA CCATCCGCGC CACTGTCACC CAGTTCAACA 
     2341 GTGTGGCCAA CTGTGTCATC ACCACCTGCC TCGGGAACCG AAGCACGAAA GCCCCAGACA 
     2401 GGGCCAGGGT GGTGGAGCAC TGGATCGAGG TGGCCAGGGA GTGCCGGATC CTCAAGAACT 
     2461 TCTCGTCACT GTATGCCATC CTCTCTGCCC TGCAGAGCAA CTCCATCCAC CGTCTGAAGA 
     2521 AGACGTGGGA AGACGTTTCC AGGGACAGTT TCCGGATCTT TCAGAAGCTG TCAGAGATCT 
     2581 TCTCAGATGA GAACAACTAC TCATTGAGCC GGGAGCTGCT CATCAAGGAG GGCACCTCCA 
     2641 AGTTTGCCAC CCTGGAGATG AACCCCAAGA GAGCCCAGAA ACGGCCGAAG GAGACGGGCA 
     2701 TCATCCAGGG CACCGTTCCC TACCTGGGCA CGTTCCTCAC CGACCTGGTG ATGCTGGACA 
     2761 CTGCCATGAA GGACTATCTG TATGGCAGAC TCATCAACTT TGAGAAGAGG AGGAAGGAGT 
     2821 TCGAGGTGAT CGCCCAGATC AAGCTGCTGC AGTCGGCCTG CAACAACTAC AGCATCGCGC 
     2881 CAGATGAGCA ATTTGGGGCC TGGTTCCGGG CCGTGGAGCG GCTCAGCGAG ACTGAGAGCT 
     2941 ACAACCTGTC GTGCGAGCTG GAGCCCCCAT CCGAGTCAGC CAGCAACACC CTCAGGACCA 
     3001 AGAAGAACAC AGCCATTGTC AAGCGCTGGA GCGACCGCCA GGCCCCCAGC ACTGAGCTCA 
     3061 GTACCAGTGG CAGCTCCCAC TCCAAGTCCT GTGACCAGCT CAGGTGTGGC CCCTACCTCA 
     3121 GCAGCGGGGA CATCGCTGAC GCGCTCAGCG TGCACTCGGC CGGCTCCTCT AGCTCCGACG 
     3181 TGGAGGAGAT CAACATCAGC TTCGTCCCGG AGTCTCCTGA TGGCCAGGAA AAGAAGTTCT 
     3241 GGGAATCAGC CTCACAGTCA TCCCCGGAGA CCTCCGGCAT CAGCTCAGCC TCCAGCAGCA 
     3301 CCTCGTCCTC CTCAGCCTCC ACCACGCCCG TGGCTGCCAC ACGCACCCAC AAGCGCTCTG 
     3361 TCTCAGGGCT CTGCAACTCC AGCTCCGCGC TGCCGCTCTA CAACCAGCAG GTGGGCGACT 
     3421 GCTGTATCAT CCGCGTCAGC CTGGACGTGG ACAATGGCAA CATGTACAAG AGCATCCTGG 
     3481 TGACCAGCCA AGATAAGGCT CCGGCTGTAA TCCGCAAGGC CATGGACAAA CACAACCTGG 
     3541 AGGAGGAGGA GCCGGAGGAC TATGAGCTGC TGCAGATTCT CTCAGATGAC CGGAAGCTGA 
     3601 AGATCCCTGA AAACGCCAAC GTCTTCTATG CCATGAACTC TACCGCCAAC TATGACTTTG 
     3661 TCCTCAAGAA GCGGACCTTC ACCAAGGGAG TGAAGGTCAA GCACGGAGCC AGCTCCACCC 
     3721 TCCCTCGCAT GAAGCAGAAA GGACTCAAGA TTGCCAAGGG CATCTTCACG CGTACGCGGC 
     3781 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     3841 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     3901 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     3961 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4021 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4081 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4141 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4201 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4261 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4321 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4381 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4441 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4501 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     4561 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     4621 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     4681 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     4741 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     4801 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     4861 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     4921 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     4981 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5041 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5101 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5161 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5221 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5281 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     5341 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     5401 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     5461 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     5521 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     5581 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     5641 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     5701 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     5761 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     5821 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     5881 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     5941 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6001 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6061 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6121 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6181 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6241 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6301 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6361 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6421 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     6481 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     6541 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     6601 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     6661 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     6721 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     6781 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     6841 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     6901 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     6961 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7021 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7081 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7141 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7201 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7261 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7321 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7381 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7441 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     7501 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     7561 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
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