LOCUS       RC235012                7590 bp    DNA     circular  SYN 
DEFINITION  AKAP1 (Myc-DDK tagged) - Homo sapiens A kinase (PRKA) anchor protein 1 (AKAP1), transcript variant 2
ACCESSION   NM_001242902
VERSION     NM_001242902.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3747..3838 
                     /label="Myc-DDK"  
     misc_feature    3868..4453 
                     /label="PolyA signal"  
     misc_feature    3925..3944 
                     /label="Restriction Enzyme"  
     misc_feature    4729..5401 
                     /label="ColE1"  
     misc_feature    5831..6625 
                     /label="Kan/Neo"  
     misc_feature    6660..6962 
                     /label="SV40 ori"  
     misc_feature    7048..7525 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3729..3735 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3729 
                     /label="AKAP1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAATCCAG TTCCGTTCGC TCTTCCCCTT GGCATTGCCT GGGATGCTGG 
     1081 CGCTCCTCGG CTGGTGGTGG TTTTTCTCTC GTAAAAAAGG CCATGTCAGC AGCCATGATG 
     1141 AGCAGCAGGT GGAGGCTGGT GCTGTGCAGC TGAGGGCTGA CCCTGCCATC AAGGAACCTC 
     1201 TCCCCGTGGA AGACGTCTGT CCCAAAGTAG TGTCCACACC CCCCAGTGTC ACAGAGCCTC 
     1261 CAGAAAAGGA ACTGTCCACC GTGAGCAAGC TGCCTGCAGA GCCCCCAGCA TTGCTCCAGA 
     1321 CACACCCACC TTGCCGAAGA TCAGAGTCCT CGGGCATTCT TCCTAACACC ACAGACATGA 
     1381 GATTGCGACC AGGAACACGC AGAGATGACA GTACAAAGCT GGAGCTAGCC CTGACAGGTG 
     1441 GTGAAGCCAA ATCGATTCCT CTAGAGTGCC CCCTTTCATC CCCAAAGGGT GTACTATTCT 
     1501 CCAGCAAATC AGCTGAGGTG TGTAAGCAAG ATTCCCCCTT CAGCAGGGTG CCAAGGAAGG 
     1561 TCCAGCCAGG CTACCCCGTA GTCCCCGCAG AGAAGCGTAG CTCTGGGGAG AGGGCAAGAG 
     1621 AGACAGGTGG GGCCGAAGGG ACTGGTGATG CCGTGTTGGG GGAAAAGGTG CTTGAAGAAG 
     1681 CTCTGTTGTC TCGGGAGCAT GTCTTGGAAT TGGAGAACAG CAAGGGCCCC AGCCTGGCCT 
     1741 CTTTAGAGGG GGAAGAAGAT AAGGGGAAGA GCAGCTCATC CCAGGTGGTG GGGCCAGTGC 
     1801 AGGAGGAAGA GTATGTAGCA GAGAAGTTGC CAAGTAGGTT CATCGAGTCG GCTCACACAG 
     1861 AGCTGGCAAA GGACGATGCG GCGCCAGCAC CCCCAGTCGC AGACGCCAAA GCCCAGGATA 
     1921 GAGGTGTCGA GGGAGAACTG GGCAATGAGG AGAGCTTGGA TAGAAATGAG GAGGGCTTGG 
     1981 ATAGAAATGA GGAGGGCTTG GATAGAAATG AGGAGAGCTT GGATAGAAAT GAGGAGGGCT 
     2041 TGGATAGAAA TGAGGAGATT AAGCGGGCTG CCTTCCAGAT AATCTCCCAA GTGATCTCAG 
     2101 AAGCAACCGA ACAGGTGCTG GCCACCACGG TTGGCAAGGT TGCAGGTCGT GTGTGTCAGG 
     2161 CCAGTCAGCT CCAAGGGCAG AAGGAAGAGA GCTGTGTCCC AGTTCACCAG AAAACTGTCT 
     2221 TGGGCCCAGA CACTGCGGAG CCTGCCACAG CAGAGGCAGC TGTTGCCCCG CCGGATGCTG 
     2281 GCCTCCCCTT GCCAGGCCTA CCAGCAGAGG GCTCACCACC ACCAAAGACC TACGTGAGCT 
     2341 GCCTGAAGAG CCTTCTGTCC AGCCCCACCA AGGACAGTAA GCCAAATATC TCTGCACACC 
     2401 ACATCTCCCT GGCCTCCTGC CTGGCACTGA CCACCCCCAG TGAAGAGTTG CCGGACCGGG 
     2461 CAGGCATCCT GGTGGAAGAT GCCACCTGTG TCACCTGCAT GTCAGACAGC AGCCAAAGTG 
     2521 TCCCTTTGGT GGCTTCTCCA GGACACTGCT CAGATTCTTT CAGCACTTCA GGGCTTGAAG 
     2581 ACTCTTGCAC AGAGACCAGC TCGAGCCCCA GGGACAAGGC CATCACCCCG CCACTGCCAG 
     2641 AAAGTACTGT GCCCTTCAGC AATGGGGTGC TGAAGGGGGA GTTGTCAGAC TTGGGGGCTG 
     2701 AGGATGGATG GACCATGGAT GCGGAAGCAG ATCATTCAGG AGGTTCTGAC AGGAACAGCA 
     2761 TGGATTCCGT GGATAGCTGT TGCAGTCTCA AGAAGACTGA GAGCTTCCAA AATGCCCAGG 
     2821 CAGGCTCCAA CCCTAAGAAG GTCGACCTCA TCATCTGGGA GATCGAGGTG CCAAAGCACT 
     2881 TAGTCGGTCG GCTAATTGGC AAGCAGGGGC GCTATGTGAG TTTTCTGAAG CAAACATCTG 
     2941 GTGCCAAGAT CTACATTTCA ACCCTGCCTT ACACCCAGAG CGTCCAGATC TGCCACATAG 
     3001 AAGGCTCTCA ACATCATGTA GACAAAGCGC TGAACTTGAT TGGGAAGAAG TTCAAAGAGC 
     3061 TGAACCTCAC CAATATCTAC GCTCCCCCAT TGCCTTCACT GGCACTGCCT TCTCTGCCGA 
     3121 TGACATCCTG GCTCATGCTG CCTGATGGCA TCACCGTGGA GGTCATTGTG GTCAACCAGG 
     3181 TCAATGCCGG GCACCTGTTC GTGCAGCAGC ACACACACCC TACCTTCCAC GCGCTGCGCA 
     3241 GCCTCGACCA GCAGATGTAC CTCTGTTACT CTCAGCCTGG AATCCCCACC TTGCCCACCC 
     3301 CAGTGGAAAT AACGGTCATC TGTGCCGCCC CTGGTGCGGA CGGGGCCTGG TGGCGAGCCC 
     3361 AAGTGGTTGC CTCCTACGAG GAGACCAACG AAGTGGAGAT TCGATACGTG GACTACGGCG 
     3421 GATATAAGAG GGTGAAAGTA GACGTGCTCC GGCAAATCAG GTCTGACTTT GTCACCCTGC 
     3481 CGTTTCAGGG AGCAGAAGTC CTTCTGGACA GTGTGATGCC CCTGTCAGAC GATGACCAGT 
     3541 TTTCACCGGA AGCAGATGCC GCCATGAGCG AGATGACGGG GAATACAGCA CTGCTTGCTC 
     3601 AGGTGACAAG TTACAGTCCA ACTGGTCTTC CTCTGATTCA GCTGTGGAGT GTGGTTGGAG 
     3661 ATGAAGTGGT GTTGATAAAC CGGTCCCTGG TGGAGCGAGG CCTTGCCCAG TGGGTAGACA 
     3721 GCTACTACAC AAGCCTTACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     3781 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     3841 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     3901 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     3961 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4021 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4081 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4141 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4201 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4261 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4321 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     4381 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     4441 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     4501 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     4561 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     4621 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     4681 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     4741 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     4801 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     4861 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     4921 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     4981 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5041 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5101 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5161 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     5221 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     5281 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     5341 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     5401 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     5461 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     5521 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     5581 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     5641 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     5701 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     5761 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     5821 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     5881 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     5941 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6001 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6061 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6121 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6181 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     6241 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     6301 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     6361 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     6421 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     6481 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     6541 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     6601 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     6661 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     6721 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     6781 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     6841 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     6901 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6961 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7021 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7081 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7141 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7201 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     7261 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     7321 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     7381 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     7441 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     7501 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     7561 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//