LOCUS       RC235009                7587 bp    DNA     circular  SYN 
DEFINITION  RALGDS (Myc-DDK tagged) - Homo sapiens ral guanine nucleotide dissociation stimulator (RALGDS), transcript variant 5
ACCESSION   NM_001271776
VERSION     NM_001271776.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3744..3835 
                     /label="Myc-DDK"  
     misc_feature    3865..4450 
                     /label="PolyA signal"  
     misc_feature    3922..3941 
                     /label="Restriction Enzyme"  
     misc_feature    4726..5398 
                     /label="ColE1"  
     misc_feature    5828..6622 
                     /label="Kan/Neo"  
     misc_feature    6657..6959 
                     /label="SV40 ori"  
     misc_feature    7045..7522 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3726..3732 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3726 
                     /label="RALGDS"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGCAGCGC ATGTGGGCCG AGGCGGCCGG GCCTGCTGGC GGCGCCGAGC 
     1081 CGCTGTTTCC GGGCTCCCGG CGGAGCCGCA GCGTGTGGGA CGCCGTGCGC CTGGAGGTGG 
     1141 GCGTCCCCGA CAGCTGCCCG GTGGTGCTGC ACAGCTTCAC GCAGCTAGAC CCCGACCTGC 
     1201 CGCGCCCGGA GAGCTCCACG CAGGAGATCG GTGAGGAGCT GATCAACGGA GTCATCTACT 
     1261 CCATCTCCCT GCGCAAGGTG CAGCTGCACC ACGGAGGCAA CAAGGGGCAG CGCTGGCTCG 
     1321 GGTATGAGAA TGAGTCGGCC CTGAACCTTT ATGAGACTTG CAAGGTGCGG ACCGTGAAGG 
     1381 CTGGCACGCT GGAGAAGCTG GTGGAGCACC TGGTGCCAGC CTTCCAGGGC AGCGACCTCT 
     1441 CCTACGTCAC CATCTTCCTG TGTACCTATA GAGCCTTCAC CACCACCCAA CAGGTCCTGG 
     1501 ACCTGCTGTT CAAAAGATAC GGCTGCATCC TCCCCTATTC CGACGAGGAT GGTGGACCCC 
     1561 AGGACCAACT TAAAAATGCC ATCTCCTCCA TCCTGGGCAC CTGGCTGGAC CAGTACTCGG 
     1621 AGGATTTCTG TCAACCTCCG GACTTTCCCT GCCTCAAGCA GCTGGTGGCC TACGTGCAGC 
     1681 TCAACATGCC AGGCTCAGAC CTGGAGCGCC GTGCCCACCT TCTCCTGGCC CAGCTGGAGC 
     1741 ACTCGGAACC CATTGAGGCA GAGCCTGAGG CTCTGTCACC AGTGCCAGCT CTAAAACCAA 
     1801 CTCCAGAGCT CGAGCTAGCT CTAACACCAG CTCGAGCACC CAGCCCAGTG CCGGCTCCAG 
     1861 CCCCGGAGCC AGAGCCAGCT CCAACACCAG CTCCAGGTTC AGAGCTAGAA GTAGCTCCAG 
     1921 CACCAGCTCC GGAGCTCCAG CAGGCTCCAG AGCCAGCTGT GGGACTAGAA TCGGCTCCAG 
     1981 CGCCAGCTCT GGAACTAGAG CCAGCTCCAG AACAGGATCC AGCTCCCTCA CAAACTCTAG 
     2041 AGCTGGAGCC AGCTCCAGCA CCAGTTCCAT CATTACAGCC TTCCTGGCCT TCACCTGTGG 
     2101 TTGCAGAGAA CGGGCTGAGT GAGGAGAAGC CTCACCTCTT GGTGTTCCCT CCAGATCTGG 
     2161 TGGCAGAGCA GTTTACACTG ATGGATGCGG AACTGTTCAA GAAGGTGGTG CCCTACCACT 
     2221 GCCTGGGCTC CATCTGGTCC CAGCGGGACA AGAAGGGCAA GGAGCACCTG GCGCCCACCA 
     2281 TCCGCGCCAC TGTCACCCAG TTCAACAGTG TGGCCAACTG TGTCATCACC ACCTGCCTCG 
     2341 GGAACCGAAG CACGAAAGCC CCAGACAGGG CCAGGGTGGT GGAGCACTGG ATCGAGGTGG 
     2401 CCAGGGAGTG CCGGATCCTC AAGAACTTCT CGTCACTGTA TGCCATCCTC TCTGCCCTGC 
     2461 AGAGCAACTC CATCCACCGT CTGAAGAAGA CGTGGGAAGA CGTTTCCAGG GACAGTTTCC 
     2521 GGATCTTTCA GAAGCTGTCA GAGATCTTCT CAGATGAGAA CAACTACTCA TTGAGCCGGG 
     2581 AGCTGCTCAT CAAGGAGGGC ACCTCCAAGT TTGCCACCCT GGAGATGAAC CCCAAGAGAG 
     2641 CCCAGAAACG GCCGAAGGAG ACGGGCATCA TCCAGGGCAC CGTTCCCTAC CTGGGCACGT 
     2701 TCCTCACCGA CCTGGTGATG CTGGACACTG CCATGAAGGA CTATCTGTAT GGCAGACTCA 
     2761 TCAACTTTGA GAAGAGGAGG AAGGAGTTCG AGGTGATCGC CCAGATCAAG CTGCTGCAGT 
     2821 CGGCCTGCAA CAACTACAGC ATCGCGCCAG ATGAGCAATT TGGGGCCTGG TTCCGGGCCG 
     2881 TGGAGCGGCT CAGCGAGACT GAGAGCTACA ACCTGTCGTG CGAGCTGGAG CCCCCATCCG 
     2941 AGTCAGCCAG CAACACCCTC AGGACCAAGA AGAACACAGC CATTGTCAAG CGCTGGAGCG 
     3001 ACCGCCAGGC CCCCAGCACT GAGCTCAGTA CCAGTGGCAG CTCCCACTCC AAGTCCTGTG 
     3061 ACCAGCTCAG GTGTGGCCCC TACCTCAGCA GCGGGGACAT CGCTGACGCG CTCAGCGTGC 
     3121 ACTCGGCCGG CTCCTCTAGC TCCGACGTGG AGGAGATCAA CATCAGCTTC GTCCCGGAGT 
     3181 CTCCTGATGG CCAGGAAAAG AAGTTCTGGG AATCAGCCTC ACAGTCATCC CCGGAGACCT 
     3241 CCGGCATCAG CTCAGCCTCC AGCAGCACCT CGTCCTCCTC AGCCTCCACC ACGCCCGTGG 
     3301 CTGCCACACG CACCCACAAG CGCTCTGTCT CAGGGCTCTG CAACTCCAGC TCCGCGCTGC 
     3361 CGCTCTACAA CCAGCAGGTG GGCGACTGCT GTATCATCCG CGTCAGCCTG GACGTGGACA 
     3421 ATGGCAACAT GTACAAGAGC ATCCTGGTGA CCAGCCAAGA TAAGGCTCCG GCTGTAATCC 
     3481 GCAAGGCCAT GGACAAACAC AACCTGGAGG AGGAGGAGCC GGAGGACTAT GAGCTGCTGC 
     3541 AGATTCTCTC AGATGACCGG AAGCTGAAGA TCCCTGAAAA CGCCAACGTC TTCTATGCCA 
     3601 TGAACTCTAC CGCCAACTAT GACTTTGTCC TCAAGAAGCG GACCTTCACC AAGGGAGTGA 
     3661 AGGTCAAGCA CGGAGCCAGC TCCACCCTCC CTCGCATGAA GCAGAAAGGA CTCAAGATTG 
     3721 CCAAGGGCAT CTTCACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     3781 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     3841 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     3901 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     3961 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4021 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4081 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4141 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4201 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4261 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4321 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     4381 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     4441 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     4501 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     4561 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     4621 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     4681 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     4741 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     4801 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     4861 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     4921 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     4981 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5041 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5101 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     5161 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     5221 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     5281 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     5341 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     5401 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     5461 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     5521 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     5581 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     5641 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     5701 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     5761 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     5821 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     5881 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     5941 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6001 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6061 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6121 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6181 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     6241 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     6301 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     6361 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     6421 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     6481 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     6541 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     6601 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     6661 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     6721 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     6781 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     6841 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     6901 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     6961 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7021 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7081 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7141 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7201 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     7261 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     7321 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     7381 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     7441 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     7501 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     7561 ATTTAACAAA AATTTAACGC GAATTTT
//