LOCUS       RC234985                7503 bp    DNA     circular  SYN 
DEFINITION  TPO (Myc-DDK tagged) - Homo sapiens thyroid peroxidase (TPO), transcript variant 7
ACCESSION   NM_001206745
VERSION     NM_001206745.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3658..3749 
                     /label="Myc-DDK"  
     misc_feature    3779..4364 
                     /label="PolyA signal"  
     misc_feature    3836..3855 
                     /label="Restriction Enzyme"  
     misc_feature    4640..5312 
                     /label="ColE1"  
     misc_feature    5742..6536 
                     /label="Kan/Neo"  
     misc_feature    6571..6873 
                     /label="SV40 ori"  
     misc_feature    6959..7436 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3648..3656 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3648 
                     /label="TPO"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGAGCGCTC GCTGTGCTGT CTGTCACGCT GGTTATGGCC TGCACAGAAG 
     1081 CCTTCTTCCC CTTCATCTCG AGAGGGAAAG AACTCCTTTG GGGAAAGCCT GAGGAGTCTC 
     1141 GTGTCTCTAG CGTCTTGGAG GAAAGCAAGC GCCTGGTGGA CACCGCCATG TACGCCACGA 
     1201 TGCAGAGAAA CCTCAAGAAA AGAGGAATCC TTTCTCCAGC TCAGCTTCTG TCTTTTTCCA 
     1261 AACTTCCTGA GCCAACAAGC GGAGTGATTG CCCGAGCAGC AGAGATAATG GAAACATCAA 
     1321 TACAAGCGAT GAAAAGAAAA GTCAACCTGA AAACTCAACA ATCACAGCAT CCAACGGATG 
     1381 CTTTATCAGA AGATCTGCTG AGCATCATTG CAAACATGTC TGGATGTCTC CCTTACATGC 
     1441 TGCCCCCAAA ATGCCCAAAC ACTTGCCTGG CGAACAAATA CAGGCCCATC ACAGGAGCTT 
     1501 GCAACAACAG AGACCACCCC AGATGGGGCG CCTCCAACAC GGCCCTGGCA CGATGGCTCC 
     1561 CTCCAGTCTA TGAGGACGGC TTCAGTCAGC CCCGAGGCTG GAACCCCGGC TTCTTGTACA 
     1621 ACGGGTTCCC ACTGCCCCCG GTCCGGGAGG TGACAAGACA TGTCATTCAA GTTTCAAATG 
     1681 AGGTTGTCAC AGATGATGAC CGCTATTCTG ACCTCCTGAT GGCATGGGGA CAATACATCG 
     1741 ACCACGACAT CGCGTTCACA CCACAGAGCA CCAGCAAAGC TGCCTTCGGG GGAGGGGCTG 
     1801 ACTGCCAGAT GACTTGTGAG AACCAAAACC CATGTTTTCC CATACAACTC CCGGAGGAGG 
     1861 CCCGGCCGGC CGCGGGCACC GCCTGTCTGC CCTTCTACCG CTCTTCGGCC GCCTGCGGCA 
     1921 CCGGGGACCA AGGCGCGCTC TTTGGGAACC TGTCCACGGC CAACCCGCGG CAGCAGATGA 
     1981 ACGGGTTGAC CTCGTTCCTG GACGCGTCCA CCGTGTATGG CAGCTCCCCG GCCCTAGAGA 
     2041 GGCAGCTGCG GAACTGGACC AGTGCCGAAG GGCTGCTCCG CGTCCACGCG CGCCTCCGGG 
     2101 ACTCCGGCCG CGCCTACCTG CCCTTCGTGC CGCCACGCGC GCCTGCGGCC TGTGCGCCCG 
     2161 AGCCCGGCAT CCCCGGAGAG ACCCGCGGGC CCTGCTTCCT GGCCGGAGAC GGCCGCGCCA 
     2221 GCGAGGTCCC CTCCCTGACG GCACTGCACA CGCTGTGGCT GCGCGAGCAC AACCGCCTGG 
     2281 CCGCGGCGCT CAAGGCCCTC AATGCGCACT GGAGCGCGGA CGCCGTGTAC CAGGAGGCGC 
     2341 GCAAGGTCGT GGGCGCTCTG CACCAGATCA TCACCCTGAG GGATTACATC CCCAGGATCC 
     2401 TGGGACCCGA GGCCTTCCAG CAGTACGTGG GTCCCTATGA AGGCTATGAC TCCACCGCCA 
     2461 ACCCCACTGT GTCCAACGTG TTCTCCACAG CCGCCTTCCG CTTCGGCCAT GCCACGATCC 
     2521 ACCCGCTGGT GAGGAGGCTG GACGCCAGCT TCCAGGAGCA CCCCGACCTG CCCGGGCTGT 
     2581 GGCTGCACCA GGCTTTCTTC AGCCCATGGA CATTACTCCG TGGAGGTTAC AATGAGTGGA 
     2641 GGGAGTTCTG CGGCCTGCCT CGCCTGGAGA CCCCCGCTGA CCTGAGCACA GCCATCGCCA 
     2701 GCAGGAGCGT GGCCGACAAG ATCCTGGACT TGTACAAGCA TCCTGACAAC ATCGATGTCT 
     2761 GGCTGGGAGG CTTAGCTGAA AACTTCCTCC CCAGGGCTCG GACAGGGCCC CTGTTTGCCT 
     2821 GTCTCATTGG GAAGCAGATG AAGGCTCTGC GGGACGGTGA CTGGTTTTGG TGGGAGAACA 
     2881 GCCACGTCTT CACGGATGCA CAGAGGCGTG AGCTGGAGAA GCACTCCCTG TCTCGGGTCA 
     2941 TCTGTGACAA CACTGGCCTC ACCAGGGTGC CCATGGATGC CTTCCAAGTC GGCAAATTCC 
     3001 CCGAAGACTT TGAGTCTTGT GACAGCATCA CTGGCATGAA CCTGGAGGCC TGGAGGGAAA 
     3061 CCTTTCCTCA AGACGACAAG TGTGGCTTCC CAGAGAGCGT GGAGAATGGG GACTTTGTGC 
     3121 ACTGTGAGGA GTCTGGGAGG CGCGTGCTGG TGTATTCCTG CCGGCACGGG TATGAGCTCC 
     3181 AAGGCCGGGA GCAGCTCACT TGCACCCAGG AAGGATGGGA TTTCCAGCCT CCCCTCTGCA 
     3241 AAGATGTGAA CGAGTGTGCA GACGGTGCCC ACCCCCCCTG CCACGCCTCT GCGAGGTGCA 
     3301 GAAACACCAA AGGCGGCTTC CAGTGTCTCT GCGCGGACCC CTACGAGTTA GGAGACGATG 
     3361 GGAGAACCTG CGTAGACTCC GGGAGGCTCC CTCGGGTGAC TTGGATCTCC ATGTCGCTGG 
     3421 CTGCTCTGCT GATCGGAGGC TTCGCAGGTC TCACCTCGAC GGTGATTTGC AGGTGGACAC 
     3481 GCACTGGCAC TAAATCCACA CTGCCCATCT CGGAGACAGG CGGAGGAACT CCCGAGCTGA 
     3541 GATGCGGAAA GCACCAGGCC GTAGGGACCT CACCGCAGCG GGCCGCAGCT CAGGACTCGG 
     3601 AGCAGGAGAG TGCTGGGATG GAAGGCCGGG ATACTCACAG GCTGCCGAGA GCCCTCACGC 
     3661 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     3721 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     3781 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     3841 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     3901 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     3961 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4021 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4081 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4141 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4201 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4261 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4321 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4381 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     4441 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     4501 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     4561 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     4621 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     4681 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     4741 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     4801 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     4861 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     4921 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     4981 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5041 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5101 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5161 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     5221 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     5281 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     5341 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     5401 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     5461 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     5521 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     5581 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     5641 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     5701 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     5761 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     5821 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     5881 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     5941 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6001 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6061 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6121 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6181 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     6241 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     6301 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     6361 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     6421 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     6481 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     6541 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     6601 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     6661 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     6721 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     6781 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     6841 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     6901 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     6961 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7021 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7081 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7141 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7201 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     7261 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     7321 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     7381 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     7441 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     7501 TTT
//