LOCUS       RC234887                7260 bp    DNA     circular  SYN 
DEFINITION  TRPC1 (Myc-DDK tagged) - Homo sapiens transient receptor potential cation channel, subfamily C, member 1 (TRPC1), transcript variant 1
ACCESSION   NM_001251845
VERSION     NM_001251845.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3417..3508 
                     /label="Myc-DDK"  
     misc_feature    3538..4123 
                     /label="PolyA signal"  
     misc_feature    3595..3614 
                     /label="Restriction Enzyme"  
     misc_feature    4399..5071 
                     /label="ColE1"  
     misc_feature    5501..6295 
                     /label="Kan/Neo"  
     misc_feature    6330..6632 
                     /label="SV40 ori"  
     misc_feature    6718..7195 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3399..3405 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3399 
                     /label="TRPC1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGGCGGCC CTGTACCCGA GCACGGACCT CTCGGGCGCC TCCTCCTCCT 
     1081 CCCTGCCTTC CTCTCCATCC TCTTCCTCGC CGAACGAGGT GATGGCGCTG AAGGATGTGC 
     1141 GGGAGGTGAA GGAGGAGAAT ACGCTGAATG AGAAGCTTTT CTTGCTGGCG TGCGACAAGG 
     1201 GTGACTATTA TATGGTTAAA AAGATTTTGG AGGAAAACAG TTCAGGTGAC TTGAACATAA 
     1261 ATTGCGTAGA TGTGCTTGGG AGAAATGCTG TTACCATAAC TATTGAAAAC GAAAACTTGG 
     1321 ATATACTGCA GCTTCTTTTG GACTACGGTT GTCAGTCTGC AGATGCACTT TTGGTGGCAA 
     1381 TCGACTCTGA AGTAGTGGGA GCTGTTGATA TACTACTTAA TCATCGACCA AAACGATCAT 
     1441 CAAGACCAAC TATAGTAAAA CTAATGGAAC GAATTCAGAA TCCTGAGTAT TCAACAACTA 
     1501 TGGATGTTGC ACCTGTCATT TTAGCTGCTC ATCGTAACAA CTATGAAATT CTTACAATGC 
     1561 TCTTAAAACA GGATGTATCT CTACCCAAGC CCCATGCAGT TGGCTGTGAA TGCACATTGT 
     1621 GTTCTGCAAA AAACAAAAAG GATAGCCTCC GGCATTCCAG GTTTCGTCTT GATATATATC 
     1681 GATGTTTGGC CAGTCCAGCT CTAATAATGT TAACAGAGGA GGATCCAATT CTGAGAGCAT 
     1741 TTGAACTTAG TGCTGATTTA AAAGAACTAA GTCTTGTGGA GGTGGAATTC AGGAATGATT 
     1801 ATGAGGAACT AGCCCGGCAA TGTAAAATGT TTGCTAAGGA TTTACTTGCA CAAGCCCGGA 
     1861 ATTCTCGTGA ATTGGAAGTT ATTCTAAACC ATACGTCTAG TGACGAGCCT CTTGACAAAC 
     1921 GGGGATTATT AGAAGAAAGA ATGAATTTAA GTCGTCTAAA ACTTGCTATC AAATATAACC 
     1981 AGAAAGAGTT TGTCTCCCAG TCTAACTGCC AGCAGTTCCT GAACACTGTT TGGTTTGGAC 
     2041 AGATGTCGGG TTACCGACGC AAGCCCACCT GTAAGAAGAT AATGACTGTT TTGACAGTAG 
     2101 GCATCTTTTG GCCAGTTTTG TCACTTTGTT ATTTGATAGC TCCCAAATCT CAGTTTGGCA 
     2161 GAATCATTCA CACACCTTTT ATGAAATTTA TCATTCATGG AGCATCATAT TTCACATTTC 
     2221 TGCTGTTGCT TAATCTATAC TCTCTTGTCT ACAATGAGGA TAAGAAAAAC ACAATGGGGC 
     2281 CAGCCCTTGA AAGAATAGAC TATCTTCTTA TTCTGTGGAT TATTGGGATG ATTTGGTCAG 
     2341 ACATTAAAAG ACTCTGGTAT GAAGGGTTGG AAGACTTTTT AGAAGAATCT CGTAATCAAC 
     2401 TCAGTTTTGT CATGAATTCT CTTTATTTGG CAACCTTTGC CCTCAAAGTG GTTGCTCACA 
     2461 ACAAGTTTCA TGATTTTGCT GATCGGAAGG ATTGGGATGC ATTCCATCCT ACACTGGTGG 
     2521 CAGAAGGGCT TTTTGCATTT GCAAATGTTC TAAGTTATCT TCGTCTCTTT TTTATGTATA 
     2581 CAACCAGCTC TATCTTGGGT CCATTACAGA TTTCAATGGG ACAGATGTTA CAAGATTTTG 
     2641 GAAAATTTCT TGGGATGTTT CTTCTTGTTT TGTTTTCTTT CACAATTGGA CTGACACAAC 
     2701 TGTATGATAA AGGATATACT TCAAAGGAGC AGAAGGACTG TGTAGGCATC TTCTGTGAAC 
     2761 AGCAAAGCAA TGATACCTTC CATTCGTTCA TTGGCACCTG CTTTGCTTTG TTCTGGTATA 
     2821 TTTTCTCCTT AGCGCATGTG GCAATCTTTG TCACAAGATT TAGCTATGGA GAAGAACTGC 
     2881 AGTCCTTTGT GGGAGCTGTC ATTGTTGGTA CATACAATGT CGTGGTTGTG ATTGTGCTTA 
     2941 CCAAACTGCT GGTGGCAATG CTTCATAAAA GCTTTCAGTT GATAGCAAAT CATGAAGACA 
     3001 AAGAATGGAA GTTTGCTCGA GCAAAATTAT GGCTTAGCTA CTTTGATGAC AAATGTACGT 
     3061 TACCTCCACC TTTCAACATC ATTCCCTCAC CAAAGACTAT CTGCTATATG ATTAGTAGCC 
     3121 TCAGTAAGTG GATTTGCTCT CATACATCAA AAGGCAAGGT CAAACGGCAA AACAGTTTAA 
     3181 AGGAATGGAG GAATTTGAAA CAGAAGAGAG ATGAAAACTA TCAAAAAGTG ATGTGCTGCC 
     3241 TAGTGCATCG TTACTTGACT TCCATGAGAC AGAAGATGCA AAGTACAGAT CAGGCAACTG 
     3301 TGGAAAATCT AAACGAACTG CGCCAAGATC TGTCAAAATT CCGAAATGAA ATAAGGGATT 
     3361 TACTTGGCTT TCGGACTTCT AAATATGCTA TGTTTTATCC AAGAAATACG CGTACGCGGC 
     3421 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     3481 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     3541 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     3601 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     3661 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     3721 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     3781 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     3841 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     3901 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     3961 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4021 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4081 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4141 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     4201 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     4261 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     4321 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     4381 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     4441 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     4501 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     4561 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     4621 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     4681 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     4741 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     4801 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     4861 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     4921 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     4981 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     5041 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     5101 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     5161 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     5221 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     5281 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     5341 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     5401 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     5461 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     5521 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     5581 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     5641 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     5701 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     5761 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     5821 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     5881 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     5941 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6001 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6061 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     6121 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     6181 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     6241 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     6301 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     6361 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     6421 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     6481 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     6541 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     6601 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     6661 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     6721 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     6781 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     6841 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     6901 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     6961 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7021 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7081 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     7141 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     7201 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//