LOCUS       RC234884                7254 bp    DNA     circular  SYN 
DEFINITION  TRPV3 (Myc-DDK tagged) - Homo sapiens transient receptor potential cation channel, subfamily V, member 3 (TRPV3), transcript variant 1
ACCESSION   NM_001258205
VERSION     NM_001258205.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3411..3502 
                     /label="Myc-DDK"  
     misc_feature    3532..4117 
                     /label="PolyA signal"  
     misc_feature    3589..3608 
                     /label="Restriction Enzyme"  
     misc_feature    4393..5065 
                     /label="ColE1"  
     misc_feature    5495..6289 
                     /label="Kan/Neo"  
     misc_feature    6324..6626 
                     /label="SV40 ori"  
     misc_feature    6712..7189 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3393..3399 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3393 
                     /label="TRPV3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAAGCCCAC CCCAAGGAGA TGGTGCCTCT CATGGGCAAG AGAGTTGCTG 
     1081 CCCCCAGTGG GAACCCTGCC ATCCTGCCAG AGAAGAGGCC GGCGGAGATC ACCCCCACAA 
     1141 AGAAGAGTGC ACACTTCTTC CTGGAGATAG AAGGGTTTGA ACCCAACCCC ACAGTTGCCA 
     1201 AGACCTCTCC TCCTGTCTTC TCCAAGCCCA TGGATTCCAA CATCCGGCAG TGCATCTCTG 
     1261 GTAACTGTGA TGACATGGAC TCCCCCCAGT CTCCTCAGGA TGATGTGACA GAGACCCCAT 
     1321 CCAATCCCAA CAGCCCCAGT GCACAGCTGG CCAAGGAAGA GCAGAGGAGG AAAAAGAGGC 
     1381 GGCTGAAGAA GCGCATCTTT GCAGCCGTGT CTGAGGGCTG CGTGGAGGAG TTGGTAGAGT 
     1441 TGCTGGTGGA GCTGCAGGAG CTTTGCAGGC GGCGCCATGA TGAGGATGTG CCTGACTTCC 
     1501 TCATGCACAA GCTGACGGCC TCCGACACGG GGAAGACCTG CCTGATGAAG GCCTTGTTAA 
     1561 ACATCAACCC CAACACCAAG GAGATAGTGC GGATCCTGCT TGCCTTTGCT GAAGAGAACG 
     1621 ACATCCTGGG CAGGTTCATC AACGCCGAGT ACACAGAGGA GGCCTATGAA GGGCAGACGG 
     1681 CGCTGAACAT CGCCATCGAG CGGCGGCAGG GGGACATCGC AGCCCTGCTC ATCGCCGCCG 
     1741 GCGCCGACGT CAACGCGCAC GCCAAGGGGG CCTTCTTCAA CCCCAAGTAC CAACACGAAG 
     1801 GCTTCTACTT CGGTGAGACG CCCCTGGCCC TGGCAGCATG CACCAACCAG CCCGAGATTG 
     1861 TGCAGCTGCT GATGGAGCAC GAGCAGACGG ACATCACCTC GCGGGACTCA CGAGGCAACA 
     1921 ACATCCTTCA CGCCCTGGTG ACCGTGGCCG AGGACTTCAA GACGCAGAAT GACTTTGTGA 
     1981 AGCGCATGTA CGACATGATC CTACTGCGGA GTGGCAACTG GGAGCTGGAG ACCACTCGCA 
     2041 ACAACGATGG CCTCACGCCG CTGCAGCTGG CCGCCAAGAT GGGCAAGGCG GAGATCCTGA 
     2101 AGTACATCCT CAGTCGTGAG ATCAAGGAGA AGCGGCTCCG GAGCCTGTCC AGGAAGTTCA 
     2161 CCGACTGGGC GTACGGACCC GTGTCATCCT CCCTCTACGA CCTCACCAAC GTGGACACCA 
     2221 CCACGGACAA CTCAGTGCTG GAAATCACTG TCTACAACAC CAACATCGAC AACCGGCATG 
     2281 AGATGCTGAC CCTGGAGCCG CTGCACACGC TGCTGCATAT GAAGTGGAAG AAGTTTGCCA 
     2341 AGCACATGTT CTTTCTGTCC TTCTGCTTTT ATTTCTTCTA CAACATCACC CTGACCCTCG 
     2401 TCTCGTACTA CCGCCCCCGG GAGGAGGAGG CCATCCCGCA CCCCTTGGCC CTGACGCACA 
     2461 AGATGGGGTG GCTGCAGCTC CTAGGGAGGA TGTTTGTGCT CATCTGGGCC ATGTGCATCT 
     2521 CTGTGAAAGA GGGCATTGCC ATCTTCCTGC TGAGACCCTC GGATCTGCAG TCCATCCTCT 
     2581 CGGATGCCTG GTTCCACTTT GTCTTTTTTA TCCAAGCTGT GCTTGTGATA CTGTCTGTCT 
     2641 TCTTGTACTT GTTTGCCTAC AAAGAGTACC TCGCCTGCCT CGTGCTGGCC ATGGCCCTGG 
     2701 GCTGGGCGAA CATGCTCTAC TATACGCGGG GTTTCCAGTC CATGGGCATG TACAGCGTCA 
     2761 TGATCCAGAA GGTCATTTTG CATGATGTTC TGAAGTTCTT GTTTGTATAT ATCGTGTTTT 
     2821 TGCTTGGATT TGGAGTAGCC TTGGCCTCGC TGATCGAGAA GTGTCCCAAA GACAACAAGG 
     2881 ACTGCAGCTC CTACGGCAGC TTCAGCGACG CAGTGCTGGA ACTCTTCAAG CTCACCATAG 
     2941 GCCTGGGTGA CCTGAACATC CAGCAGAACT CCAAGTATCC CATTCTCTTT CTGTTCCTGC 
     3001 TCATCACCTA TGTCATCCTC ACCTTTGTTC TCCTCCTCAA CATGCTCATT GCTCTGATGG 
     3061 GCGAGACTGT GGAGAACGTC TCCAAGGAGA GCGAACGCAT CTGGCGCCTG CAGAGAGCCA 
     3121 GGACCATCTT GGAGTTTGAG AAAATGTTAC CAGAATGGCT GAGGAGCAGA TTCCGGATGG 
     3181 GAGAGCTGTG CAAAGTGGCC GAGGATGATT TCCGACTGTG TTTGCGGATC AATGAGGTGA 
     3241 AGTGGACTGA ATGGAAGACG CACGTCTCCT TCCTTAACGA AGACCCGGGG CCTGTAAGAC 
     3301 GAACAGCAGA TTTCAACAAA ATCCAAGATT CTTCCAGGAA CAACAGCAAA ACCACTCTCA 
     3361 ATGCATTTGA AGAAGTCGAG GAATTCCCGG AAACCTCGGT GACGCGTACG CGGCCGCTCG 
     3421 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     3481 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     3541 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     3601 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     3661 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     3721 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     3781 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     3841 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     3901 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     3961 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4021 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4081 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4141 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4201 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     4261 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     4321 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     4381 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     4441 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     4501 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     4561 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     4621 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     4681 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     4741 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     4801 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     4861 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     4921 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     4981 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5041 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5101 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     5161 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     5221 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     5281 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     5341 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     5401 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     5461 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     5521 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     5581 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     5641 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     5701 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     5761 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     5821 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     5881 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     5941 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6001 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6061 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6121 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6181 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     6241 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     6301 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     6361 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     6421 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     6481 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6541 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     6601 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     6661 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     6721 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     6781 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     6841 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     6901 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     6961 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7021 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7081 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7141 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7201 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//