LOCUS       RC234869                7203 bp    DNA     circular  SYN 
DEFINITION  CUL3 (Myc-DDK tagged) - Homo sapiens cullin 3 (CUL3), transcript variant 3
ACCESSION   NM_001257198
VERSION     NM_001257198.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3360..3451 
                     /label="Myc-DDK"  
     misc_feature    3481..4066 
                     /label="PolyA signal"  
     misc_feature    3538..3557 
                     /label="Restriction Enzyme"  
     misc_feature    4342..5014 
                     /label="ColE1"  
     misc_feature    5444..6238 
                     /label="Kan/Neo"  
     misc_feature    6273..6575 
                     /label="SV40 ori"  
     misc_feature    6661..7138 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3342..3348 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3342 
                     /label="CUL3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTAAGAATT CACTTCTTTT CATTTAGTTT TAATTTGTCT CATGATGTAG 
     1081 TTCTCTCAGT AATCACAGAA CCTTCAAGAT GTATGACCAT GGATGAAAAA TATGTAAACA 
     1141 GCATTTGGGA CCTTCTGAAA AATGCAATTC AAGAAATCCA GCGTAAGAAT AACAGTGGTC 
     1201 TTAGTTTTGA GGAGCTCTAT AGAAATGCAT ATACAATGGT TTTGCATAAA CATGGAGAAA 
     1261 AGCTCTACAC TGGACTAAGA GAAGTTGTTA CCGAACATCT CATAAATAAG GTGCGAGAAG 
     1321 ATGTACTAAA TTCATTGAAT AACAACTTTC TTCAAACGCT AAATCAAGCT TGGAATGATC 
     1381 ATCAAACAGC TATGGTGATG ATTAGAGACA TACTAATGTA CATGGACCGT GTGTATGTAC 
     1441 AACAAAATAA TGTGGAGAAC GTCTACAATT TGGGATTAAT TATTTTTCGA GATCAAGTTG 
     1501 TACGTTATGG GTGTATTAGG GATCATCTAC GGCAAACTCT ATTGGATATG ATTGCAAGAG 
     1561 AGCGGAAAGG AGAAGTCGTA GACAGAGGCG CAATAAGAAA TGCTTGCCAG ATGTTAATGA 
     1621 TTTTAGGTCT CGAAGGAAGA TCAGTCTATG AAGAAGATTT TGAGGCTCCT TTTTTGGAAA 
     1681 TGTCTGCAGA ATTTTTTCAG ATGGAAAGCC AGAAATTTTT AGCAGAAAAT AGTGCTTCAG 
     1741 TATATATAAA GAAAGTAGAA GCTAGAATTA ATGAAGAAAT AGAACGAGTG ATGCACTGCC 
     1801 TTGACAAATC AACGGAAGAA CCAATTGTAA AGGTGGTTGA AAGGGAACTC ATTTCCAAGC 
     1861 ACATGAAGAC TATAGTAGAA ATGGAGAATT CTGGGCTAGT ACATATGTTG AAAAATGGAA 
     1921 AGACAGAAGA CCTTGGTTGC ATGTACAAGT TATTTAGTCG TGTGCCAAAT GGTTTGAAAA 
     1981 CAATGTGTGA GTGTATGAGT TCCTATTTGA GGGAGCAAGG TAAAGCTCTT GTTTCTGAAG 
     2041 AAGGAGAAGG AAAGAATCCT GTTGACTATA TCCAGGGCTT ATTGGATCTG AAGAGTAGGT 
     2101 TCGATCGCTT CCTCCTGGAA TCATTCAACA ATGACCGTCT CTTTAAACAA ACTATTGCGG 
     2161 GTGACTTTGA GTATTTTCTC AACCTCAACT CCAGGTCTCC TGAATACCTC TCATTATTTA 
     2221 TTGATGATAA GCTGAAAAAG GGAGTCAAAG GGCTAACAGA ACAAGAAGTA GAAACAATAT 
     2281 TGGATAAAGC AATGGTCCTT TTTAGGTTTA TGCAAGAAAA AGATGTATTT GAACGTTATT 
     2341 ATAAACAACA CTTGGCAAGG AGACTTCTCA CAAATAAAAG TGTTTCTGAT GACTCTGAAA 
     2401 AAAACATGAT ATCTAAGTTA AAGACTGAAT GTGGATGTCA GTTCACGTCA AAACTGGAAG 
     2461 GAATGTTTAG GGATATGAGC ATCTCAAACA CAACGATGGA TGAATTCAGG CAACATCTAC 
     2521 AGGCAACTGG TGTATCTTTA GGTGGTGTTG ATCTTACAGT CCGGGTGCTC ACGACAGGAT 
     2581 ATTGGCCCAC TCAGTCAGCC ACACCAAAGT GCAACATCCC ACCAGCACCA AGACATGCTT 
     2641 TTGAGATATT CAGAAGGTTC TACTTAGCCA AACACAGTGG TCGACAGCTC ACACTCCAGC 
     2701 ATCATATGGG TTCTGCAGAT CTCAATGCCA CATTTTATGG ACCAGTTAAA AAGGAAGATG 
     2761 GATCTGAAGT TGGTGTTGGA GGTGCACAAG TAACTGGCTC TAATACACGG AAGCACATAT 
     2821 TGCAAGTTTC CACTTTCCAG ATGACCATAT TAATGCTCTT TAATAATAGA GAAAAATACA 
     2881 CATTTGAGGA AATTCAGCAA GAGACAGATA TCCCTGAAAG AGAGCTTGTT AGAGCCCTAC 
     2941 AGTCCCTCGC CTGTGGTAAA CCAACACAGC GGGTTCTTAC AAAAGAACCC AAATCAAAGG 
     3001 AAATAGAAAA TGGTCATATA TTTACAGTTA ATGATCAATT CACATCCAAA CTACACAGAG 
     3061 TCAAGATTCA AACAGTTGCT GCCAAACAAG GTGAATCCGA CCCAGAGAGG AAAGAAACAA 
     3121 GGCAGAAAGT AGACGACGAC AGAAAACATG AGATAGAAGC TGCTATAGTG CGGATAATGA 
     3181 AATCTAGAAA GAAGATGCAG CACAATGTTC TAGTAGCGGA GGTAACTCAG CAGTTGAAGG 
     3241 CGCGATTCTT ACCAAGTCCA GTTGTTATTA AGAAACGTAT TGAAGGACTT ATTGAGAGAG 
     3301 AATATTTGGC ACGAACACCT GAGGATCGCA AAGTATACAC ATATGTAGCA ACGCGTACGC 
     3361 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     3421 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     3481 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     3541 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     3601 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     3661 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     3721 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     3781 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     3841 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     3901 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     3961 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4021 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4081 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     4141 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     4201 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     4261 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     4321 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     4381 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     4441 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     4501 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     4561 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     4621 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     4681 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     4741 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     4801 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     4861 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     4921 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     4981 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     5041 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     5101 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     5161 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     5221 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     5281 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     5341 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     5401 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     5461 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     5521 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     5581 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     5641 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     5701 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     5761 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     5821 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     5881 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     5941 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     6001 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     6061 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     6121 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     6181 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     6241 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     6301 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     6361 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     6421 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     6481 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     6541 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     6601 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     6661 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     6721 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     6781 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     6841 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     6901 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     6961 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     7021 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     7081 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     7141 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     7201 TTT
//