LOCUS       RC234744                6987 bp    DNA     circular  SYN 
DEFINITION  CUL3 (Myc-DDK tagged) - Homo sapiens cullin 3 (CUL3), transcript variant 2
ACCESSION   NM_001257197
VERSION     NM_001257197.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3144..3235 
                     /label="Myc-DDK"  
     misc_feature    3265..3850 
                     /label="PolyA signal"  
     misc_feature    3322..3341 
                     /label="Restriction Enzyme"  
     misc_feature    4126..4798 
                     /label="ColE1"  
     misc_feature    5228..6022 
                     /label="Kan/Neo"  
     misc_feature    6057..6359 
                     /label="SV40 ori"  
     misc_feature    6445..6922 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3126..3132 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3126 
                     /label="CUL3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGAATCTG AGCAAAGGCA CGGGCAGCCG GAAGGACACC AAGATGCGGA 
     1081 TCCGGGCCTT TCCGGTGCGA GAAGATGTAC TAAATTCATT GAATAACAAC TTTCTTCAAA 
     1141 CGCTAAATCA AGCTTGGAAT GATCATCAAA CAGCTATGGT GATGATTAGA GACATACTAA 
     1201 TGTACATGGA CCGTGTGTAT GTACAACAAA ATAATGTGGA GAACGTCTAC AATTTGGGAT 
     1261 TAATTATTTT TCGAGATCAA GTTGTACGTT ATGGGTGTAT TAGGGATCAT CTACGGCAAA 
     1321 CTCTATTGGA TATGATTGCA AGAGAGCGGA AAGGAGAAGT CGTAGACAGA GGCGCAATAA 
     1381 GAAATGCTTG CCAGATGTTA ATGATTTTAG GTCTCGAAGG AAGATCAGTC TATGAAGAAG 
     1441 ATTTTGAGGC TCCTTTTTTG GAAATGTCTG CAGAATTTTT TCAGATGGAA AGCCAGAAAT 
     1501 TTTTAGCAGA AAATAGTGCT TCAGTATATA TAAAGAAAGT AGAAGCTAGA ATTAATGAAG 
     1561 AAATAGAACG AGTGATGCAC TGCCTTGACA AATCAACGGA AGAACCAATT GTAAAGGTGG 
     1621 TTGAAAGGGA ACTCATTTCC AAGCACATGA AGACTATAGT AGAAATGGAG AATTCTGGGC 
     1681 TAGTACATAT GTTGAAAAAT GGAAAGACAG AAGACCTTGG TTGCATGTAC AAGTTATTTA 
     1741 GTCGTGTGCC AAATGGTTTG AAAACAATGT GTGAGTGTAT GAGTTCCTAT TTGAGGGAGC 
     1801 AAGGTAAAGC TCTTGTTTCT GAAGAAGGAG AAGGAAAGAA TCCTGTTGAC TATATCCAGG 
     1861 GCTTATTGGA TCTGAAGAGT AGGTTCGATC GCTTCCTCCT GGAATCATTC AACAATGACC 
     1921 GTCTCTTTAA ACAAACTATT GCGGGTGACT TTGAGTATTT TCTCAACCTC AACTCCAGGT 
     1981 CTCCTGAATA CCTCTCATTA TTTATTGATG ATAAGCTGAA AAAGGGAGTC AAAGGGCTAA 
     2041 CAGAACAAGA AGTAGAAACA ATATTGGATA AAGCAATGGT CCTTTTTAGG TTTATGCAAG 
     2101 AAAAAGATGT ATTTGAACGT TATTATAAAC AACACTTGGC AAGGAGACTT CTCACAAATA 
     2161 AAAGTGTTTC TGATGACTCT GAAAAAAACA TGATATCTAA GTTAAAGACT GAATGTGGAT 
     2221 GTCAGTTCAC GTCAAAACTG GAAGGAATGT TTAGGGATAT GAGCATCTCA AACACAACGA 
     2281 TGGATGAATT CAGGCAACAT CTACAGGCAA CTGGTGTATC TTTAGGTGGT GTTGATCTTA 
     2341 CAGTCCGGGT GCTCACGACA GGATATTGGC CCACTCAGTC AGCCACACCA AAGTGCAACA 
     2401 TCCCACCAGC ACCAAGACAT GCTTTTGAGA TATTCAGAAG GTTCTACTTA GCCAAACACA 
     2461 GTGGTCGACA GCTCACACTC CAGCATCATA TGGGTTCTGC AGATCTCAAT GCCACATTTT 
     2521 ATGGACCAGT TAAAAAGGAA GATGGATCTG AAGTTGGTGT TGGAGGTGCA CAAGTAACTG 
     2581 GCTCTAATAC ACGGAAGCAC ATATTGCAAG TTTCCACTTT CCAGATGACC ATATTAATGC 
     2641 TCTTTAATAA TAGAGAAAAA TACACATTTG AGGAAATTCA GCAAGAGACA GATATCCCTG 
     2701 AAAGAGAGCT TGTTAGAGCC CTACAGTCCC TCGCCTGTGG TAAACCAACA CAGCGGGTTC 
     2761 TTACAAAAGA ACCCAAATCA AAGGAAATAG AAAATGGTCA TATATTTACA GTTAATGATC 
     2821 AATTCACATC CAAACTACAC AGAGTCAAGA TTCAAACAGT TGCTGCCAAA CAAGGTGAAT 
     2881 CCGACCCAGA GAGGAAAGAA ACAAGGCAGA AAGTAGACGA CGACAGAAAA CATGAGATAG 
     2941 AAGCTGCTAT AGTGCGGATA ATGAAATCTA GAAAGAAGAT GCAGCACAAT GTTCTAGTAG 
     3001 CGGAGGTAAC TCAGCAGTTG AAGGCGCGAT TCTTACCAAG TCCAGTTGTT ATTAAGAAAC 
     3061 GTATTGAAGG ACTTATTGAG AGAGAATATT TGGCACGAAC ACCTGAGGAT CGCAAAGTAT 
     3121 ACACATATGT AGCAACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     3181 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     3241 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     3301 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     3361 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     3421 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     3481 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     3541 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     3601 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     3661 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     3721 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     3781 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     3841 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     3901 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     3961 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     4021 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     4081 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     4141 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     4201 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     4261 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     4321 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     4381 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     4441 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     4501 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     4561 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     4621 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     4681 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     4741 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     4801 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     4861 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     4921 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     4981 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     5041 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     5101 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     5161 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     5221 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     5281 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     5341 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     5401 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     5461 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     5521 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     5581 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     5641 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     5701 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     5761 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     5821 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     5881 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     5941 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     6001 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     6061 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     6121 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     6181 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     6241 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     6301 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     6361 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     6421 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     6481 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     6541 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     6601 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     6661 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     6721 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     6781 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     6841 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     6901 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     6961 ATTTAACAAA AATTTAACGC GAATTTT
//