LOCUS       RC233213                9402 bp    DNA     circular  SYN 
DEFINITION  GEMIN5 (Myc-DDK tagged) - Homo sapiens gem (nuclear organelle) associated protein 5 (GEMIN5), transcript variant 2
ACCESSION   NM_001252156
VERSION     NM_001252156.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5559..5650 
                     /label="Myc-DDK"  
     misc_feature    5680..6265 
                     /label="PolyA signal"  
     misc_feature    5737..5756 
                     /label="Restriction Enzyme"  
     misc_feature    6541..7213 
                     /label="ColE1"  
     misc_feature    7643..8437 
                     /label="Kan/Neo"  
     misc_feature    8472..8774 
                     /label="SV40 ori"  
     misc_feature    8860..9337 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5541..5547 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5541 
                     /label="GEMIN5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGCAGGAG CCGCGGACGC TGCCGCCCTC CCCCAACTGG TACTGCGCCC 
     1081 GCTGCAGCGA TGCCGTGCCC GGGGGCCTCT TTGGCTTCGC CGCGCGGACC TCCGTCTTCC 
     1141 TTGTCCGCGT GGGCCCGGGC GCAGGCGAGA GTCCAGGGAC ACCCCCGTTT CGAGTCATAG 
     1201 GAGAGTTGGT GGGACACACC GAAAGGGTCT CTGGCTTCAC ATTTTCTCAT CACCCTGGTC 
     1261 AGTACAACCT CTGTGCCACC AGCTCCGACG ATGGGACTGT GAAAATATGG GATGTAGAGA 
     1321 CAAAAACAGT TGTGACAGAA CATGCACTCC ATCAGCATAC GATATCAACA TTACATTGGT 
     1381 CTCCTCGAGT AAAGGACTTA ATAGTATCTG GGGATGAAAA AGGAGTAGTT TTCTGTTACT 
     1441 GGTTTAACAG AAATGACAGC CAGCACCTCT TTATAGAACC CAGGACAATT TTCTGTCTTA 
     1501 CTTGTTCACC TCATCATGAA GATTTAGTAG CCATTGGCTA CAAGGATGGC ATAGTGGTGA 
     1561 TAATTGACAT CAGTAAGAAA GGAGAAGTTA TTCATAGGCT TCGAGGCCAT GATGATGAAA 
     1621 TCCACTCCAT AGCCTGGTGT CCCCTGCCTG GTGAAGATTG TTTATCTATA AACCAAGAGG 
     1681 AAACTTCAGA AGCTGAAATT ACCAACGGGA ATGCTGTAGC ACAAGCTCCA GTAACAAAAG 
     1741 GTTGCTACTT AGCCACTGGA AGCAAAGATC AAACCATTCG AATCTGGAGC TGTTCTAGAG 
     1801 GCCGAGGGGT GATGATTTTG AAATTGCCCT TTCTGAAGAG AAGAGGAGGG GGTATAGACC 
     1861 CAACTGTTAA AGAGCGCCTT TGGTTGACAC TCCATTGGCC CAGCAATCAA CCAACACAGC 
     1921 TGGTATCTAG CTGTTTTGGA GGTGAACTGT TGCAATGGGA TCTCACTCAA TCTTGGAGAC 
     1981 GGAAATACAC CCTCTTCAGT GCCTCATCAG AAGGGCAAAA TCATTCAAGA ATTGTGTTTA 
     2041 ATTTATGTCC TTTACAAACA GAGGATGACA AACAGCTATT ACTTTCTACA TCAATGGATA 
     2101 GAGATGTAAA ATGTTGGGAC ATAGCCACCT TGGAGTGCAG CTGGACCCTT CCTTCCCTTG 
     2161 GTGGGTTTGC ATACAGCCTG GCTTTCTCTT CTGTGGACAT AGGCTCTTTG GCCATAGGTG 
     2221 TTGGGGATGG CATGATCCGT GTATGGAATA CACTCTCCAT AAAGAACAAC TATGATGTGA 
     2281 AAAATTTTTG GCAAGGCGTG AAGTCCAAGG TTACAGCGCT GTGCTGGCAC CCAACCAAGG 
     2341 AAGGTTGCTT AGCTTTTGGA ACTGATGATG GAAAAGTGGG ATTGTATGAC ACCTACTCCA 
     2401 ACAAGCCTCC ACAGATTTCT AGCACATATC ATAAGAAGAC TGTATATACT TTAGCCTGGG 
     2461 GGCCACCAGT ACCCCCCATG TCACTTGGAG GAGAAGGAGA CAGACCTTCC CTTGCTTTAT 
     2521 ACAGCTGTGG AGGAGAAGGG ATTGTCTTAC AGCATAATCC CTGGAAGCTT AGTGGAGAAG 
     2581 CCTTTGACAT CAACAAACTC ATCAGGGACA CCAATTCAAT CAAATACAAA TTGCCTGTAC 
     2641 ACACAGAGAT AAGTTGGAAA GCAGATGGCA AAATCATGGC TCTTGGCAAT GAAGATGGAT 
     2701 CAATAGAAAT ATTTCAGATT CCCAACCTGA AACTGATCTG TACTATCCAA CAGCATCACA 
     2761 AGCTTGTGAA TACCATTAGC TGGCATCATG AGCATGGCAG CCAGCCAGAA TTGAGCTATC 
     2821 TGATGGCCTC TGGCTCCAAC AATGCAGTCA TTTACGTGCA CAACCTGAAG ACTGTCATAG 
     2881 AGAGCAGCCC TGAGTCTCCA GTGACCATTA CAGAGCCCTA CCGGACCCTC TCAGGGCATA 
     2941 CGGCCAAGAT TACCAGTGTG GCGTGGAGCC CACATCATGA TGGAAGGCTG GTATCTGCTT 
     3001 CCTATGATGG TACAGCCCAG GTGTGGGATG CTCTCCGGGA AGAGCCCCTG TGCAATTTCC 
     3061 GAGGACATCG AGGTCGACTG CTTTGTGTGG CATGGTCTCC TTTGGATCCA GACTGCATCT 
     3121 ATTCAGGGGC AGATGACTTT TGTGTGCACA AGTGGCTCAC TTCCATGCAA GATCATTCCC 
     3181 GGCCTCCTCA AGGCAAAAAA AGTATTGAAT TGGAGAAAAA ACGGCTCTCT CAACCTAAGG 
     3241 CAAAGCCCAA AAAGAAGAAA AAGCCCACCT TGAGAACTCC TGTAAAGCTG GAATCGATTG 
     3301 ATGGAAATGA AGAAGAAAGC ATGAAGGAGA ACTCAGGACC TGTTGAGAAT GGTGTGTCAG 
     3361 ACCAAGAAGG GGAGGAGCAA GCACGGGAGC CGGAATTACC CTGTGGCCTT GCTCCAGCGG 
     3421 TTTCTAGAGA ACCAGTTATC TGCACTCCAG TTTCCTCAGG CTTTGAAAAG TCAAAAGTCA 
     3481 CCATTAATAA CAAAGTCATT TTACTGAAAA AGGAGCCACC AAAAGAGAAG CCAGAAACCT 
     3541 TAATCAAGAA GAGAAAAGCT CGTTCCTTGC TTCCCCTGAG TACAAGCCTG GACCACAGAT 
     3601 CCAAAGAGGA GCTTCATCAG GACTGTTTGG TACTAGCAAC TGCAAAGCAC TCCAGAGAGC 
     3661 TGAATGAAGA TGTGTCTGCT GATGTTGAGG AAAGATTTCA TCTGGGGCTT TTCACAGACA 
     3721 GGGCTACCCT GTATAGAATG ATTGATATTG AAGGAAAAGG TCACTTAGAA AATGGCCACC 
     3781 CTGAGTTATT TCACCAGCTT ATGCTTTGGA AAGGAGATCT CAAAGGTGTT CTCCAGACTG 
     3841 CAGCAGAAAG AGGGGAGCTG ACAGACAACC TTGTGGCTAT GGCACCAGCA GCTGGCTACC 
     3901 ATGTGTGGCT ATGGGCTGTG GAAGCTTTTG CCAAACAGCT GTGTTTTCAG GATCAGTATG 
     3961 TCAAGGCTGC TTCTCACCTA CTTTCCATCC ACAAAGTGTA TGAAGCGGTG GAGCTGCTCA 
     4021 AGTCAAACCA TTTTTACAGG GAAGCTATTG CGATTGCCAA GGCCCGGCTG CGCCCGGAGG 
     4081 ACCCAGTCCT GAAGGACTTG TACCTCAGCT GGGGAACCGT CCTAGAAAGA GATGGCCACT 
     4141 ATGCTGTAGC TGCCAAATGC TATTTAGGGG CCACTTGTGC TTATGATGCA GCCAAAGTTT 
     4201 TGGCCAAAAA GGGGGATGCG GCATCACTTA GAACGGCTGC AGAGTTGGCT GCCATCGTAG 
     4261 GAGAGGATGA GTTGTCTGCT TCCCTGGCTC TCAGATGTGC CCAAGAGCTG CTTCTGGCCA 
     4321 ACAACTGGGT GGGAGCCCAG GAAGCCCTGC AGCTGCATGA AAGTCTACAG GGTCAGAGAT 
     4381 TGGTGTTTTG CCTTCTGGAG CTACTGTCCA GGCATCTGGA GGAAAAGCAG CTTTCAGAGG 
     4441 GCAAAAGCTC CTCCTCTTAC CACACTTGGA ACACGGGCAC CGAAGGGCCT TTCGTGGAGA 
     4501 GGGTGACTGC AGTGTGGAAG AGCATCTTCA GCCTTGACAC CCCTGAGCAG TATCAGGAAG 
     4561 CCTTTCAGAA GCTGCAGAAC ATCAAGTACC CATCTGCTAC AAATAACACA CCTGCCAAAC 
     4621 AGCTCCTGCT TCACATTTGC CATGACTTGA CCCTGGCAGT GCTGAGCCAA CAGATGGCCT 
     4681 CCTGGGACGA GGCTGTGCAG GCGCTCCTTC GGGCGGTGGT CCGGAGCTAT GACTCAGGGA 
     4741 GCTTCACCAT CATGCAGGAA GTGTACTCAG CCTTTCTCCC TGACGGCTGT GACCACCTAA 
     4801 GAGACAAGTT GGGGGACCAT CAATCCCCTG CCACACCAGC TTTCAAAAGT TTGGAGGCCT 
     4861 TTTTTCTTTA TGGGCGTCTG TATGAATTCT GGTGGTCTCT CTCCAGACCT TGCCCAAATT 
     4921 CCAGTGTCTG GGTAAGGGCT GGTCACAGAA CACTCTCTGT TGAGCCAAGC CAGCAGTTAG 
     4981 ACACTGCCAG CACTGAAGAA ACGGACCCTG AAACTTCTCA GCCAGAGCCA AACAGGCCTT 
     5041 CAGAACTAGA CTTGAGACTC ACAGAAGAAG GTGAGCGAAT GCTGAGTACT TTTAAGGAGC 
     5101 TCTTTTCAGA AAAGCATGCC AGTCTCCAAA ACTCACAGAG AACTGTTGCT GAAGTCCAAG 
     5161 AGACCTTGGC AGAAATGATC CGACAACACC AAAAGAGTCA ACTCTGTAAA TCCACAGCAA 
     5221 ATGGTCCTGA TAAGAATGAA CCGGAAGTAG AAGCAGAGCA GCCCCTCTGC AGTTCTCAGA 
     5281 GCCAGTGTAA AGAAGAAAAA AATGAGCCAC TTTCTCTGCC TGAGTTAACC AAAAGGCTTA 
     5341 CCGAGGCAAA TCAGAGAATG GCGAAATTTC CTGAGAGCAT TAAGGCCTGG CCCTTCCCAG 
     5401 ATGTGCTGGA GTGCTGCCTC GTCCTGCTTC TCATCAGGTC CCACTTTCCT GGCTGTCTGG 
     5461 CCCAGGAAAT GCAGCAGCAG GCCCAAGAGC TCCTTCAGAA ATACGGCAAC ACGAAAACTT 
     5521 ACAGAAGACA CTGCCAGACC TTCTGTATGA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     5581 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     5641 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     5701 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     5761 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     5821 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     5881 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     5941 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     6001 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     6061 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     6121 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     6181 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     6241 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     6301 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     6361 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     6421 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     6481 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     6541 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     6601 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     6661 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     6721 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     6781 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     6841 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     6901 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6961 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     7021 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     7081 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     7141 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     7201 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     7261 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     7321 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     7381 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     7441 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     7501 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     7561 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     7621 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     7681 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     7741 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     7801 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     7861 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     7921 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7981 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     8041 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     8101 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     8161 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     8221 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     8281 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     8341 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     8401 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     8461 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     8521 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     8581 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     8641 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8701 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8761 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     8821 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     8881 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     8941 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     9001 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     9061 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     9121 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     9181 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     9241 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     9301 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     9361 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//