LOCUS       RC233209                8727 bp    DNA     circular  SYN 
DEFINITION  PLEKHA5 (Myc-DDK tagged) - Homo sapiens pleckstrin homology domain containing, family A member 5 (PLEKHA5), transcript variant 5
ACCESSION   NM_001256470
VERSION     NM_001256470.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4884..4975 
                     /label="Myc-DDK"  
     misc_feature    5005..5590 
                     /label="PolyA signal"  
     misc_feature    5062..5081 
                     /label="Restriction Enzyme"  
     misc_feature    5866..6538 
                     /label="ColE1"  
     misc_feature    6968..7762 
                     /label="Kan/Neo"  
     misc_feature    7797..8099 
                     /label="SV40 ori"  
     misc_feature    8185..8662 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4866..4872 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4866 
                     /label="PLEKHA5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCGGAT CTGAACCTGG AGTGGATCTC CCTGCCCCGG TCCTGGACTT 
     1081 ACGGGATCAC CAGGGGCGGC CGAGTCTTCT TCATCAACGA GGAGGCCAAG AGCACCACCT 
     1141 GGCTGCACCC CGTCACCGGC GAGGCGGTGG TCACCGGACA CCGGCGGCAG AGCACAGATT 
     1201 TGCCTACTGG CTGGGAAGAA GCATATACTT TTGAAGGTGC AAGATACTAT ATAAACCATA 
     1261 ATGAAAGGAA AGTGACCTGC AAACATCCAG TCACAGGACA ACCATCACAG GACAATTGTA 
     1321 TTTTTGTAGT GAATGAACAG ACTGTTGCAA CCATGACATC TGAAGAAAAG AAGGAACGGC 
     1381 CAATAAGTAT GATAAATGAA GCTTCTAACT ATAACGTGAC TTCAGATTAT GCAGTGCATC 
     1441 CAATGAGCCC TGTAGGCAGA ACTTCACGAG CTTCAAAAAA AGTTCATAAT TTTGGAAAGA 
     1501 GGTCAAATTC AATTAAAAGG AATCCTAATG CACCGGTTGT CAGACGAGGT TGGCTTTATA 
     1561 AACAGGACAG TACTGGCATG AAATTGTGGA AGAAACGCTG GTTTGTGCTT TCTGACCTTT 
     1621 GCCTCTTTTA TTATAGAGAT GAGAAAGAAG AGGGTATCCT GGGAAGCATA CTGTTACCTA 
     1681 GTTTTCAGAT AGCTTTGCTT ACCTCTGAAG ATCACATTAA TCGCAAATAT GCTTTTAAGG 
     1741 CAGCCCATCC AAACATGCGG ACCTATTATT TCTGCACTGA TACAGGAAAG GAAATGGAGT 
     1801 TGTGGATGAA AGCCATGTTA GATGCTGCCC TAGTACAGAC AGAACCTGTG AAAAGAATTA 
     1861 CCTTTAATTT CCGAGTGGAC AAGATTACAT CTGAAAATGC ACCAACTAAA GAAACCAATA 
     1921 ACATTCCCAA CCATAGAGTG CTAATTAAAC CAGAGATCCA AAACAATCAA AAAAACAAGG 
     1981 AAATGAGCAA AATTGAAGAA AAAAAGGCAT TAGAAGCTGA AAAATATGGA TTTCAGAAGG 
     2041 ATGGTCAAGA TAGACCCTTA ACAAAAATTA ATAGTGTAAA GCTGAATTCT CTGCCATCTG 
     2101 AATATGAGAG TGGGTCAGCA TGCCCTGCTC AGACTGTGCA CTACAGACCA ATCAACTTGA 
     2161 GCAGTTCAGA GAACAAAATA GTCAATGTTA GCCTGGCAGA TCTTAGAGGT GGAAATCGCC 
     2221 CCAATACAGG GCCCTTATAC ACAGAGGCCG ATCGAGTCAT ACAGAGAACA AATTCAATGC 
     2281 AGCAGTTGGA ACAGTGGATT AAAATCCAGA AGGGGAGGGG TCATGAAGAA GAAACCAGGG 
     2341 GAGTAATTTC TTACCAAACA TTACCAAGAA ATATGCCAAG TCACAGAGCC CAGATTATGG 
     2401 CCCGCTACCC TGAAGGTTAT AGAACACTCC CAAGAAACAG CAAGACAAGG CCTGAAAGTA 
     2461 TCTGCAGTGT AACCCCTTCC ACTCATGACA AGACATTAGG ACCCGGAGCG GAGGAGAAAC 
     2521 GGAGGTCCAT GAGAGATGAC ACAATGTGGC AGCTCTACGA ATGGCAGCAG CGTCAGTTTT 
     2581 ATAACAAACA GAGCACCCTC CCTCGACACA GTACTTTGAG TAGTCCCAAA ACCATGGTAA 
     2641 ATATTTCTGA CCAGACAATG CACTCTATTC CCACATCACC TTCCCACGGG TCAATAGCTG 
     2701 CTTATCAGGG ATACTCCCCT CAACGAACTT ACAGATCGGA AGTGTCTTCA CCAATTCAGA 
     2761 GAGGAGATGT GACAATAGAC CGCAGACACA GGGCCCATCA CCCTAAGCAT GTCTATGTGC 
     2821 CTGACAGAAG GTCAGTGCCA GCTGGCCTGA CTTTACAGTC TGTTAGTCCC CAGAGCCTCC 
     2881 AAGGGAAAAC GCCAGAGGAG CTTACGCTGC TGCTAATAAA GCTGAGACGG CAGCAAGCCG 
     2941 AACTGAGTAG TATCCGGGAG CATACGTTAG CACAGCTCAT GCAGCTAAAG CTTGAGGCCC 
     3001 ACAGCCCAAA GAATGAAATT CTTTCACATC ATCTCCAAAG GAACACCATA TATTTGGATC 
     3061 ATCAGATGAA AGAAAATGAA CCTATTATCA CCATGGTTCA CACAATGATT GAGAACTCGG 
     3121 CGCTAAGACC CCAACTGTAC CAGCAATTCT TAAGACAGAA GAGCAAGATA AGTCTATATT 
     3181 GTCTGTCACA AGATGAAGGT AGAGGCACAT TATACAAATA CAGACCTGAA GAAGTAGATA 
     3241 TTGATGCCAA GTTAAGCCGA TTATGTGAAC AAGATAAAGT GGTGCATGCT CTGGAAGAGA 
     3301 AACTTCAGCA ACTCCACAAG GAGAAATACA CGCTTGAGCA AGCTTTGCTA TCAGCCAGCC 
     3361 AAGAGATAGA AATGCATGCA GATAACCCAG CAGCCATTCA GACAGTGGTG TTACAAAGGG 
     3421 ATGATTTACA AAATGGACTG CTTAGTACGT GTCGAGAACT TTCTCGAGCC ACTGCCGAAT 
     3481 TGGAACGAGC ATGGAGAGAA TATGATAAGT TAGAATACGA TGTAACTGTT ACCAGGAACC 
     3541 AGATGCAAGA GCAGCTGGAT CACCTTGGTG AAGTTCAGAC GGAATCAGCA GGAATTCAGC 
     3601 GTGCACAGAT TCAGAAAGAA CTTTGGCGAA TTCAGGATGT CATGGAAGGG CTGAGTAAAC 
     3661 ATAAGCAGCA AAGAGGTACT ACAGAAATAG GTATGATAGG ATCAAAGCCT TTCTCAACAG 
     3721 TTAAGTACAA AAATGAGGAA GAGGAAGTAG TCCCACCTCG TCCTCCACTT CCTCGGTCCT 
     3781 ATGACTTTAC AGAGCAGCCT CCCATAATCC CCCCTCTGCC CAGTGATAGC AGCTCCTTGC 
     3841 TCTGTTATAG CAGGGGCCCA GTTCATCTGC CTGAAGAAAA GAAGATGTAT CAAGTTCAAG 
     3901 GATATCCAAG AAATGGATCT CACTGTGGTC CAGATTATAG ACTCTACAAG AGTGAACCAG 
     3961 AGTTAACAAC AGTGGCAGAA GTTGATGAAT CTAATGGAGA AGAAAAATCA GAACCTGTTT 
     4021 CAGAGATAGA AACTTCAGTT GTTAAAGGTT CCCACTTTCC TGTTGGAGTA GTCCCTCCAA 
     4081 GAGCAAAATC ACCAACACCC GAATCTTCGA CAATAGCTTC CTATGTAACC TTGAGGAAAA 
     4141 CTAAGAAGAT GATGGATCTA AGAACGGAAA GACCAAGAAG TGCAGTGGAA CAGCTCTGTT 
     4201 TGGCTGAAAG TACTCGACCA AGGATGACTG TGGAAGAGCA AATGGAAAGA ATAAGAAGAC 
     4261 ATCAACAAGC GTGCCTGAGG GAGAAGAAAA AAGGGTTAAA TGTTATCGGT GCTTCAGACC 
     4321 AGTCACCCTT ACAAAGCCCT TCAAATTTAA GGGATAATCC ATTTAGGACT ACTCAGACTC 
     4381 GAAGGAGGGA TGATAAGGAA CTGGACACTG CCATTAGAGA AAATGATGTA AAGCCAGACC 
     4441 ATGAAACTCC TGCAACAGAA ATTGTTCAAC TAAAAGAAAC CGAACCCCAA AATGTGGACT 
     4501 TCAGCAAAGA GTTAAAAAAA ACTGAAAACA TTTCATATGA AATGCTTTTT GAACCTGAGC 
     4561 CAAATGGAGT AAATTCTGTG GAAATGATGG ATAAAGAAAG AAACAAAGAC AAAATGCCTG 
     4621 AGGATGTTAC ATTCAGCCCT CAAGATGAAA CACAGACCGC AAATCATAAA CCAGAAGAGC 
     4681 ATCCTGAAGA AAATACAAAG AACAGTGTTG ACGAACAGGA AGAAACTGTT ATTTCTTACG 
     4741 AATCAACTCC TGAGGTTTCT AGAGGAAATC AAACAATGGC AGTGAAAAGT CTGTCCCCAT 
     4801 CTCCTGAGTC CTCGGCATCG CCAGTTCCAT CCACTCAGCC GCAGCTCACA GAAGGATCAC 
     4861 ATTTCATGTG TGTGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4921 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4981 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     5041 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     5101 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     5161 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     5221 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     5281 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5341 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5401 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5461 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5521 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5581 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5641 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5701 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5761 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5821 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5881 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5941 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     6001 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     6061 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     6121 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     6181 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     6241 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6301 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6361 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6421 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6481 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6541 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6601 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6661 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6721 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6781 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6841 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6901 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6961 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     7021 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     7081 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     7141 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     7201 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     7261 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7321 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7381 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7441 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7501 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7561 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7621 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7681 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7741 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7801 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7861 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7921 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7981 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8041 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8101 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     8161 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     8221 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     8281 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8341 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8401 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8461 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8521 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8581 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8641 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8701 ATTTAACAAA AATTTAACGC GAATTTT
//