LOCUS       RC233205                8418 bp    DNA     circular  SYN 
DEFINITION  AMBRA1 (Myc-DDK tagged) - Homo sapiens autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant 3
ACCESSION   NM_001267783
VERSION     NM_001267783.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4575..4666 
                     /label="Myc-DDK"  
     misc_feature    4696..5281 
                     /label="PolyA signal"  
     misc_feature    4753..4772 
                     /label="Restriction Enzyme"  
     misc_feature    5557..6229 
                     /label="ColE1"  
     misc_feature    6659..7453 
                     /label="Kan/Neo"  
     misc_feature    7488..7790 
                     /label="SV40 ori"  
     misc_feature    7876..8353 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4557..4563 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4557 
                     /label="AMBRA1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGGTTGTC CCAGAAAAGA ATGCTGTCCG GATACTCTGG GGGCGAGAAC 
     1081 GGGGTGCTCG GGCCATGGGA GCTCAGCGGC TTCTGCAGGA GCTGGTAGAA GATAAAACCC 
     1141 GGTGGATGAA ATGGGAGGGC AAGAGAGTAG AACTGCCGGA TAGTCCACGC TCTACCTTCT 
     1201 TATTGGCCTT CAGCCCAGAC AGGACTCTCT TAGCCTCCAC CCATGTGAAC CATAATATCT 
     1261 ATATTACGGA GGTGAAGACT GGCAAGTGTG TTCATTCCCT GATTGGACAC CGCCGTACTC 
     1321 CATGGTGTGT CACTTTTCAT CCCACCATCT CAGGCCTTAT TGCTTCTGGC TGCCTAGATG 
     1381 GGGAGGTTAG GATTTGGGAT TTACACGGTG GCAGTGAAAG CTGGTTCACA GATAGCAACA 
     1441 ATGCCATTGC CTCCCTGGCT TTCCACCCTA CGGCTCAGCT CCTGCTGATT GCCACTGCCA 
     1501 ATGAGATCCA CTTCTGGGAC TGGAGTCGAC GGGAACCCTT TGCTGTGGTG AAGACAGCTA 
     1561 GTGAGATGGA ACGGGTCCGT CTGGTGAGAT TTGATCCACT TGGACACTAC TTACTCACAG 
     1621 CAATTGTTAA CCCCTCTAAT CAACAGGGTG ATGACGAACC AGAGATCCCC ATAGATGGAA 
     1681 CAGAATTATC CCACTACCGT CAGCGTGCCC TCCTGCAATC ACAGCCAGTT CGCCGGACGC 
     1741 CTCTCCTCCA CAATTTCCTG CACATGCTGT CCTCCCGCTC TTCTGGCATC CAGACCGAGC 
     1801 CCTTCCATCC CCCGGAGCAG GCCTCGTCAA CGCAGCAGGA CCAGGGCCTC CTGAACCGGC 
     1861 CGTCTGCCTT CAGTACAGTC CAGAGCAGCA CTGCCGGCAA CACGCTCCGC AACCTCAGTC 
     1921 TGGGTCCTAC CCGCCGCTCT TTGGGAGGGC CTCTGTCTAG CCACCCTTCT AGGTATCACC 
     1981 GAGAAATAGC TCCTGGGTTG ACAGGATCTG AGTGGACCCG GACAGTACTC AGTCTGAACT 
     2041 CCCGCTCTGA GGCGGAATCC ATGCCCCCGC CCAGAACCAG TGCCTCTTCG GTGAGTTTGC 
     2101 TGTCTGTGCT GAGACAGCAG GAAGGTGGCT CTCAGGCATC TGTGTACACT TCAGCCACAG 
     2161 AAGGGAGGGG TTTTCCGGCA TCAGGGTTGG CAACTGAGTC AGATGGAGGG AATGGCTCCA 
     2221 GCCAAAACAA CTCGGGCAGC ATTCGCCATG AGCTTCAGTG TGACCTGAGA CGCTTCTTTC 
     2281 TGGAGTATGA CCGGCTTCAG GAGCTGGATC AGAGCCTGAG TGGGGAAGCT CCCCAGACCC 
     2341 AACAGGCCCA GGAAATGCTC AACAATAACA TTGAATCTGA GAGGCCAGGC CCTTCCCACC 
     2401 AGCCCACCCC ACACAGCAGT GAGAACAACT CCAACCTGTC CCGTGGCCAC CTGAATCGCT 
     2461 GTCGTGCTTG CCACAATCTC CTGACCTTCA ACAACGATAC CCTGCGCTGG GAAAGAACCA 
     2521 CACCTAACTA CTCCTCTGGC GAGGCTAGTT CCTCTTGGCA GGTCCCCAGC TCCTTTGAGA 
     2581 GTGTGCCATC AAGTGGCAGC CAGTTGCCAC CTCTCGAGCG GACTGAGGGC CAAACGCCCA 
     2641 GCTCCAGCAG GCTGGAGTTG AGCAGCTCTG CTAGTCCGCA GGAGGAGAGG ACTGTGGGGG 
     2701 TGGCCTTTAA CCAGGAGACA GGCCACTGGG AAAGAATTTA CACCCAGTCC AGCAGATCTG 
     2761 GAACTGTGTC ACAGGAGGCC TTACATCAGG ATATGCCTGA GGAGAGCTCT GAGGAGGATT 
     2821 CACTCAGGAG ATTATCTCCT GCTGCATACT ACGCCCAGAG GATGATCCAG TATCTCTCAC 
     2881 GGAGAGACAG TATTCGCCAG CGCTCCATGC GCTACCAACA GAACCGTCTC CGTTCTTCCA 
     2941 CCTCCTCCTC TTCCTCAGAC AACCAGGGTC CATCAGTAGA GGGAACCGAC TTGGAATTTG 
     3001 AGGACTTTGA GGACAATGGT GACAGATCCA GGCACCGAGC TCCACGCAAT GCCCGGATGT 
     3061 CTGCACCTTC GCTTGGACGC TTTGTCCCAA GGCGTTTCTT GCTGCCTGAG TACTTGCCTT 
     3121 ATGCTGGGAT TTTTCATGAA CGTGGACAGC CTGGCTTGGC TACTCACTCT TCTGTTAACA 
     3181 GGGTCCTGGC AGGGGCAGTG ATCGGTGATG GACAGTCTGC TGTGGCCAGT AACATTGCCA 
     3241 ATACTACCTA CCGGCTCCAG TGGTGGGACT TCACTAAGTT TGACCTCCCT GAAATCAGTA 
     3301 ATGCTTCCGT GAATGTGCTG GTGCAGAACT GCAAGATCTA CAATGATGCC AGCTGTGACA 
     3361 TTTCTGCAGA TGGCCAGCTC CTGGCAGCTT TCATCCCCAG CAGCCAGAGG GGCTTTCCTG 
     3421 ATGAAGGCAT CCTGGCAGTG TACTCCCTGG CCCCCCATAA CCTGGGCGAA ATGCTCTACA 
     3481 CCAAGCGATT TGGTCCCAAT GCCATTTCGG TGAGCCTGTC CCCAATGGGC AGATATGTAA 
     3541 TGGTGGGCTT GGCCTCACGA AGGATCCTGC TGCACCCCTC CACAGAGCAC ATGGTGGCCC 
     3601 AGGTCTTCAG GCTGCAACAG GCCCATGGTG GAGAGACCTC CATGAGGAGA GTTTTCAACG 
     3661 TCCTTTATCC CATGCCTGCC GACCAGCGGA GACATGTCAG TATCAACTCT GCCCGTTGGC 
     3721 TGCCTGAGCC AGGGCTTGGC TTGGCCTATG GTACTAACAA AGGAGACCTG GTGATCTGCC 
     3781 GACCAGAGGC CTTAAACTCT GGTGTTGAGT ACTACTGGGA CCAGCTGAAC GAGACGGTCT 
     3841 TCACTGTCCA TTCCAACAGC AGGAGCAGCG AGCGGCCTGG AACCAGCAGA GCCACATGGA 
     3901 GGACAGACAG AGACATGGGG CTGATGAATG CCATTGGGCT TCAGCCCCGG AACCCTGCCA 
     3961 CCTCAGTGAC ATCTCAGGGC ACCCAGACTC TGGCCCTTCA GCTGCAGAAT GCCGAAACAC 
     4021 AGACTGAGAG GGAGGTGCCG GAGCCAGGGA CAGCCGCCTC AGGTCCTGGT GAAGGTGAGG 
     4081 GTTCAGAGTA TGGTGCCAGT GGAGAAGATG CGCTCAGCAG GATCCAGAGG CTGATGGCGG 
     4141 AGGGCGGCAT GACAGCCGTG GTGCAGCGGG AGCAGAGCAC CACCATGGCC TCCATGGGCG 
     4201 GCTTCGGCAA CAACATCATC GTCAGCCACC GCATTCACCG CAGCTCTCAG ACGGGCACTG 
     4261 AACCTGGTGC CGCCCACACC TCCTCACCCC AGCCCTCCAC CTCTCGGGGA CTGCTCCCAG 
     4321 AGGCCGGGCA ACTGGCAGAG CGAGGCCTAA GCCCCCGGAC AGCTTCCTGG GACCAGCCTG 
     4381 GTACCCCTGG GCGGGAGCCA ACCCAGCCAA CCCTGCCCTC TTCCTCCCCT GTCCCCATTC 
     4441 CTGTTTCCCT TCCCAGCGCT GAGGGACCAA CCCTCCACTG CGAGTTGACC AATAACAACC 
     4501 ACCTTCTGGA TGGTGGCAGC AGCAGGGGGG ACGCTGCAGG CCCTAGGGGA GAACCACGGA 
     4561 ACAGGACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4621 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4681 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4741 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4801 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4861 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4921 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4981 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5041 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5101 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5161 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5221 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5281 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5341 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5401 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5461 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5521 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5581 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5641 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5701 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5761 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5821 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5881 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     5941 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6001 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6061 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6121 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6181 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6241 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6301 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6361 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6421 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6481 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6541 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6601 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6661 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6721 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6781 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6841 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6901 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     6961 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7021 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7081 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7141 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7201 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7261 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7321 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7381 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7441 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7501 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7561 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7621 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7681 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7741 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7801 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7861 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7921 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7981 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8041 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8101 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8161 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8221 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8281 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8341 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8401 AAATTTAACG CGAATTTT
//