LOCUS       RC233202                8181 bp    DNA     circular  SYN 
DEFINITION  DGKH (Myc-DDK tagged) - Homo sapiens diacylglycerol kinase, eta (DGKH), transcript variant 4
ACCESSION   NM_001204505
VERSION     NM_001204505.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4338..4429 
                     /label="Myc-DDK"  
     misc_feature    4459..5044 
                     /label="PolyA signal"  
     misc_feature    4516..4535 
                     /label="Restriction Enzyme"  
     misc_feature    5320..5992 
                     /label="ColE1"  
     misc_feature    6422..7216 
                     /label="Kan/Neo"  
     misc_feature    7251..7553 
                     /label="SV40 ori"  
     misc_feature    7639..8116 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4320..4326 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4320 
                     /label="DGKH"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTGTGTGCT GAGAACAGAA AGGAGATGGA GGATTGGATC AGCTCACTGA 
     1081 AGTCTGTACA GACCAGAGAA CCCTACGAGG TGGCCCAGTT TAATGTGGAA CATTTCTCAG 
     1141 GGATGCACAA CTGGTACGCC TGCTCCCACG CCCGACCCAC CTTCTGTAAC GTGTGCAGAG 
     1201 AGAGTCTTTC TGGAGTCACC TCCCATGGCC TGTCCTGCGA AGTGTGTAAA TTCAAGGCTC 
     1261 ACAAAAGATG TGCAGTGAGA GCAACAAATA ACTGTAAATG GACTACCCTG GCCTCCATCG 
     1321 GGAAGGACAT TATAGAAGAT GAAGATGGCG TCGCGATGCC TCACCAGTGG CTTGAGGGCA 
     1381 ACCTGCCTGT AAGTGCCAAG TGTGCTGTCT GCGACAAAAC ATGTGGCAGT GTTCTCCGTC 
     1441 TACAGGATTG GAAATGCCTT TGGTGTAAGA CAATGGTACA CACTGCCTGC AAAGATTTAT 
     1501 ACCATCCAAT ATGTCCACTT GGTCAATGTA AAGTATCTAT CATACCTCCA ATTGCACTAA 
     1561 ACAGCACCGA TTCCGATGGT TTCTGTAGAG CAACATTTTC GTTCTGTGTT AGTCCTCTAT 
     1621 TGGTTTTTGT CAATTCTAAG AGTGGAGATA ATCAGGGAGT AAAGTTCCTC CGTCGCTTTA 
     1681 AACAGTTGCT AAATCCGGCT CAGGTGTTTG ATTTAATGAA TGGAGGTCCT CATTTAGGTT 
     1741 TAAGATTATT TCAGAAGTTT GACAATTTCC GGATTCTTGT TTGTGGAGGC GATGGAAGTG 
     1801 TAGGTTGGGT TTTGTCAGAA ATCGATAAGC TCAACTTGAA TAAACAGTGT CAGCTGGGAG 
     1861 TGTTGCCTTT GGGTACAGGA AATGACCTTG CCCGAGTTCT TGGCTGGGGA GGTTCATATG 
     1921 ACGATGACAC CCAACTTCCT CAGATCCTAG AGAAACTGGA ACGAGCCAGT ACCAAAATGT 
     1981 TGGACAGGTG GAGTATAATG ACATATGAAC TCAAATTGCC ACCAAAAGCT TCCCTACTTC 
     2041 CAGGACCTCC AGAAGCATCT GAAGAATTTT ATATGACGAT TTATGAAGAC TCAGTTGCAA 
     2101 CGCATCTTAC AAAAATCCTC AATTCTGATG AACATGCAGT GGTCATATCT TCTGCCAAGA 
     2161 CGCTATGTGA AACTGTAAAG GACTTCGTTG CCAAAGTAGA AAAGACGTAT GACAAAACCT 
     2221 TGGAAAATGC CGTTGTAGCT GATGCCGTGG CCAGTAAATG TTCAGTCCTA AACGAGAAGC 
     2281 TCGAACAACT GCTGCAGGCT TTGCACACAG ATTCCCAGGC TGCGCCTGTT CTCCCTGGCC 
     2341 TCAGCCCTCT CATTGTGGAA GAAGATGCTG TGGAATCGTC CAGTGAAGAG TCCCTGGGTG 
     2401 AAAGCAAAGA GCAGCTTGGG GATGACGTTA CAAAACCTTC CTCCCAGAAA GCCGTCAAAC 
     2461 CAAGGGAAAT CATGTTGCGG GCAAATAGTT TAAAGAAAGC AGTGAGGCAA GTCATTGAGG 
     2521 AAGCCGGAAA AGTTATGGAT GACCCGACAG TTCACCCCTG TGAACCAGCT AATCAGTCCT 
     2581 CTGATTATGA CAGCACAGAA ACAGATGAAT CTAAGGAGGA AGCTAAAGAT GATGGTGCCA 
     2641 AAGAATCAAT AACTGTTAAA ACTGCACCTC GGTCTCCAGA TGCCCGGGCA AGTTATGGCC 
     2701 ATTCCCAAAC TGATTCTGTC CCTGGTCCAG CTGTGGCAGC CAGCAAAGAA AACCTCCCTG 
     2761 TGCTCAATAC CAGAATAATC TGCCCAGGTT TAAGAGCAGG ACTGGCTGCC TCAATTGCTG 
     2821 GGAGTTCGAT TATCAACAAA ATGTTACTGG CAAACATTGA TCCTTTTGGT GCCACGCCGT 
     2881 TTATTGACCC GGATCTAGAT TCCGTAGATG GATATTCAGA AAAATGTGTC ATGAACAATT 
     2941 ACTTTGGGAT TGGATTAGAT GCAAAAATTT CATTAGAATT TAATAATAAA AGAGAGGAGC 
     3001 ACCCTGAAAA ATGCAGGAGC CGAACTAAAA ACTTGATGTG GTATGGAGTC CTTGGAACCC 
     3061 GGGAGTTATT ACAGAGATCG TACAAGAATT TAGAACAAAG GGTTCAACTT GAGTGTGATG 
     3121 GGCAGTATAT TCCTCTTCCC AGCTTGCAAG GCATAGCCGT GTTGAACATT CCCAGCTATG 
     3181 CTGGAGGCAC TAACTTTTGG GGTGGAACTA AAGAGGATGA TATATTTGCT GCACCATCCT 
     3241 TTGATGACAA GATCCTGGAA GTTGTAGCAA TATTTGATAG CATGCAAATG GCAGTTTCAA 
     3301 GGGTCATTAA ACTGCAGCAT CATCGAATAG CCCAGTGCCG TACAGTGAAA ATCACTATAT 
     3361 TTGGTGACGA AGGAGTCCCA GTGCAAGTGG ATGGTGAAGC GTGGGTTCAG CCTCCAGGGA 
     3421 TTATCAAAAT TGTGCACAAA AACAGAGCAC AAATGCTAAC AAGGGACAGA GCCTTTGAGA 
     3481 GCACTCTGAA ATCTTGGGAA GATAAGCAGA AGTGTGATTC TGGTAAACCA GTTCTCCGAA 
     3541 CCCATTTGTA CATCCATCAC GCCATTGACT TGGCAACAGA AGAGGTGTCG CAGATGCAGC 
     3601 TATGCTCCCA GGCTGCAGAG GAGCTCATTA CTAGGATATG TGACGCAGCC ACAATTCACT 
     3661 GTCTTTTGGA GCAAGAACTG GCCCATGCTG TGAATGCCTG CTCCCATGCC CTGAATAAAG 
     3721 CCAACCCAAG GTGCCCGGAG AGTCTTACAA GAGACACTGC CACTGAAATA GCCATCAATG 
     3781 TGAAGGCGCT GTATAATGAA ACAGAATCTT TGCTAGTTGG CAGGGTTCCT TTGCAGCTGG 
     3841 AATCGCCACA TGAAGAGCGA GTATCCAATG CCTTACACTC TGTGGAGGTG GAATTACAGA 
     3901 AACTGACAGA GATTCCTTGG CTTTATTATA TCTTACACCC AAATGAGGAT GAGGAACCTC 
     3961 CTATGGATTG CACCAAAAGG AACAACAGAA GCACCGTATT TCGAATAGTG CCAAAGTTTA 
     4021 AAAAGGAAAA GGTTCAGAAG CAGAAGACAA GTTCACAGCC TGTTCAGAAA TGGGGCACAG 
     4081 AGGAAGTTGC TGCTTGGCTG GATCTGCTCA ATTTGGGAGA GTACAAAGAT ATCTTCATCC 
     4141 GTCATGACAT CAGAGGGGCT GAACTTTTGC ATCTGGAAAG GCGAGATCTT AAGAACACAG 
     4201 TAGGAGAAAA AAGAGACACC AAAGAAAATG GTAAACATAT GGATCTGGGG ATACCGAAAG 
     4261 TGGGTCATGT GAAGCGAATT CTCCAGGGAA TTAAAGAGCT TGGAAGGAGC ACTCCACAGT 
     4321 CGGAGGTGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4381 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4441 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4501 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4561 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4621 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4681 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4741 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4801 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4861 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4921 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4981 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5041 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5101 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5161 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5221 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5281 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5341 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5401 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5461 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5521 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5581 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5641 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5701 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5761 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     5821 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     5881 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     5941 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6001 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6061 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6121 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6181 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6241 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6301 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6361 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6421 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6481 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6541 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6601 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6661 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6721 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6781 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6841 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     6901 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     6961 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7021 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7081 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7141 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7201 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7261 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7321 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7381 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7441 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7501 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7561 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7621 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7681 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7741 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7801 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7861 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     7921 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     7981 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8041 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8101 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8161 CAAAAATTTA ACGCGAATTT T
//