LOCUS       RC233198                8133 bp    DNA     circular  SYN 
DEFINITION  DGKH (Myc-DDK tagged) - Homo sapiens diacylglycerol kinase, eta (DGKH), transcript variant 5
ACCESSION   NM_001204506
VERSION     NM_001204506.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4290..4381 
                     /label="Myc-DDK"  
     misc_feature    4411..4996 
                     /label="PolyA signal"  
     misc_feature    4468..4487 
                     /label="Restriction Enzyme"  
     misc_feature    5272..5944 
                     /label="ColE1"  
     misc_feature    6374..7168 
                     /label="Kan/Neo"  
     misc_feature    7203..7505 
                     /label="SV40 ori"  
     misc_feature    7591..8068 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4272..4278 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4272 
                     /label="DGKH"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTGTGTGCT GAGAACAGAA AGGAGATGGA GGATTGGATC AGCTCACTGA 
     1081 AGTCTGTACA GACCAGAGAA CCCTACGAGG TGGCCCAGTT TAATGTGGAA CATTTCTCAG 
     1141 GGATGCACAA CTGGTACGCC TGCTCCCACG CCCGACCCAC CTTCTGTAAC GTGTGCAGAG 
     1201 AGAGTCTTTC TGGAGTCACC TCCCATGGCC TGTCCTGCGA AGTGTGTAAA TTCAAGGCTC 
     1261 ACAAAAGATG TGCAGTGAGA GCAACAAATA ACTGTAAATG GACTACCCTG GCCTCCATCG 
     1321 GGAAGGACAT TATAGAAGAT GAAGATGGCG TCGCGATGCC TCACCAGTGG CTTGAGGGCA 
     1381 ACCTGCCTGT AAGTGCCAAG TGTGCTGTCT GCGACAAAAC ATGTGGCAGT GTTCTCCGTC 
     1441 TACAGGATTG GAAATGCCTT TGGTGTAAGA CAATGGTACA CACTGCCTGC AAAGATTTAT 
     1501 ACCATCCAAT ATGTCCACTT GGTCAATGTA AAGTATCTAT CATACCTCCA ATTGCACTAA 
     1561 ACAGCACCGA TTCCGATGGT TTCTGTAGAG CAACATTTTC GTTCTGTGTT AGTCCTCTAT 
     1621 TGGTTTTTGT CAATTCTAAG AGTGGAGATA ATCAGGGAGT AAAGTTCCTC CGTCGCTTTA 
     1681 AACAGTTGCT AAATCCGGCT CAGGTGTTTG ATTTAATGAA TGGAGGTCCT CATTTAGGTT 
     1741 TAAGATTATT TCAGAAGTTT GACAATTTCC GGATTCTTGT TTGTGGAGGC GATGGAAGTG 
     1801 TAGGTTGGGT TTTGTCAGAA ATCGATAAGC TCAACTTGAA TAAACAGTGT CAGCTGGGAG 
     1861 TGTTGCCTTT GGGTACAGGA AATGACCTTG CCCGAGTTCT TGGCTGGGGA GGTTCATATG 
     1921 ACGATGACAC CCAACTTCCT CAGATCCTAG AGAAACTGGA ACGAGCCAGT ACCAAAATGT 
     1981 TGGACAGGTG GAGTATAATG ACATATGAAC TCAAATTGCC ACCAAAAGCT TCCCTACTTC 
     2041 CAGGACCTCC AGAAGCATCT GAAGAATTTT ATATGACGAT TTATGAAGAC TCAGTTGCAA 
     2101 CGCATCTTAC AAAAATCCTC AATTCTGATG AACATGCAGT GGTCATATCT TCTGCCAAGA 
     2161 CGCTATGTGA AACTGTAAAG GACTTCGTTG CCAAAGTAGA AAAGACGTAT GACAAAACCT 
     2221 TGGAAAATGC CGTTGTAGCT GATGCCGTGG CCAGTAAATG TTCAGTCCTA AACGAGAAGC 
     2281 TCGAACAACT GCTGCAGGCT TTGCACACAG ATTCCCAGGC TGCGCCTGTT CTCCCTGGCC 
     2341 TCAGCCCTCT CATTGTGGAA GAAGATGCTG TGGAATCGTC CAGTGAAGAG TCCCTGGGTG 
     2401 AAAGCAAAGA GCAGCTTGGG GATGACGTTA CAAAACCTTC CTCCCAGAAA GCCGTCAAAC 
     2461 CAAGGGAAAT CATGTTGCGG GCAAATAGTT TAAAGAAAGC AGTGAGGCAA GTCATTGAGG 
     2521 AAGCCGGAAA AGTTATGGAT GACCCGACAG TTCACCCCTG TGAACCAGCT AATCAGTCCT 
     2581 CTGATTATGA CAGCACAGAA ACAGATGAAT CTAAGGAGGA AGCTAAAGAT GATGGTGCCA 
     2641 AAGAATCAAT AACTGTTAAA ACTGCACCTC GGTCTCCAGA TGCCCGGGCA AGTTATGGCC 
     2701 ATTCCCAAAC TGATTCTGTC CCTGGTCCAG CTGTGGCAGC CAGCAAAGAA AACCTCCCTG 
     2761 TGCTCAATAC CAGAATAATC TGCCCAGGTT TAAGAGCAGG ACTGGCTGCC TCAATTGCTG 
     2821 GGAGTTCGAT TATCAACAAA ATGTTACTGG CAAACATTGA TCCTTTTGGT GCCACGCCGT 
     2881 TTATTGACCC GGATCTAGAT TCCGTAGATG GATATTCAGA AAAATGTGTC ATGAACAATT 
     2941 ACTTTGGGAT TGGATTAGAT GCAAAAATTT CATTAGAATT TAATAATAAA AGAGAGGAGC 
     3001 ACCCTGAAAA ATGCAGGAGC CGAACTAAAA ACTTGATGTG GTATGGAGTC CTTGGAACCC 
     3061 GGGAGTTATT ACAGAGATCG TACAAGAATT TAGAACAAAG GGTTCAACTT GAGTGTGATG 
     3121 GGCAGTATAT TCCTCTTCCC AGCTTGCAAG GCATAGCCGT GTTGAACATT CCCAGCTATG 
     3181 CTGGAGGCAC TAACTTTTGG GGTGGAACTA AAGAGGATGA TATATTTGCT GCACCATCCT 
     3241 TTGATGACAA GATCCTGGAA GTTGTAGCAA TATTTGATAG CATGCAAATG GCAGTTTCAA 
     3301 GGGTCATTAA ACTGCAGCAT CATCGAATAG CCCAGTGCCG TACAGTGAAA ATCACTATAT 
     3361 TTGGTGACGA AGGAGTCCCA GTGCAAGTGG ATGGTGAAGC GTGGGTTCAG CCTCCAGGGA 
     3421 TTATCAAAAT TGTGCACAAA AACAGAGCAC AAATGCTAAC AAGGGACAGA GCCTTTGAGA 
     3481 GCACTCTGAA ATCTTGGGAA GATAAGCAGA AGTGTGATTC TGGTAAACCA GTTCTCCGAA 
     3541 CCCATTTGTA CATCCATCAC GCCATTGACT TGGCAACAGA AGAGGTGTCG CAGATGCAGC 
     3601 TATGCTCCCA GGCTGCAGAG GAGCTCATTA CTAGGATATG TGACGCAGCC ACAATTCACT 
     3661 GTCTTTTGGA GCAAGAACTG GCCCATGCTG TGAATGCCTG CTCCCATGCC CTGAATAAAG 
     3721 CCAACCCAAG GTGCCCGGAG AGTCTTACAA GAGACACTGC CACTGAAATA GCCATCAATG 
     3781 TGAAGGCGCT GTATAATGAA ACAGAATCTT TGCTAGTTGG CAGGGTTCCT TTGCAGCTGG 
     3841 AATCGCCACA TGAAGAGCGA GTATCCAATG CCTTACACTC TGTGGAGGTG GAATTACAGA 
     3901 AACTGACAGA GATTCCTTGG CTTTATTATA TCTTACACCC AAATGAGGAT GAGGAACCTC 
     3961 CTATGGATTG CACCAAAAGG AACAACAGAA GCACCGTATT TCGAATAGTG CCAAAGTTTA 
     4021 AAAAGGAAAA GGTTCAGAAG CAGAAGACAA GTTCACAGCC TGTTCAGAAA TGGGGCACAG 
     4081 AGGAAGTTGC TGCTTGGCTG GATCTGCTCA ATTTGGGAGA GTACAAAGAT ATCTTCATCC 
     4141 GTCATGACAT CAGAGGGGCT GAACTTTTGC ATCTGGAAAG GCGAGATCTT AAGGATCTGG 
     4201 GGATACCGAA AGTGGGTCAT GTGAAGCGAA TTCTCCAGGG AATTAAAGAG CTTGGAAGGA 
     4261 GCACTCCACA GTCGGAGGTG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4321 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4381 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4441 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4501 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4561 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4621 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4681 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4741 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4801 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4861 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4921 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4981 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5041 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5101 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5161 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5221 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5281 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5341 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5401 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5461 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5521 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5581 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5641 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5701 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5761 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5821 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5881 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5941 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6001 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6061 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6121 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6181 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6241 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6301 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6361 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6421 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6481 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6541 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6601 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6661 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6721 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6781 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6841 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6901 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6961 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7021 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7081 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7141 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7201 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7261 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7321 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7381 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7441 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7501 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7561 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7621 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7681 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7741 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7801 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7861 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7921 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7981 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8041 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8101 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//