LOCUS       RC233190                7914 bp    DNA     circular  SYN 
DEFINITION  SEMA6D (Myc-DDK tagged) - Homo sapiens sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (SEMA6D), transcript variant 7
ACCESSION   NM_001198999
VERSION     NM_001198999.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4071..4162 
                     /label="Myc-DDK"  
     misc_feature    4192..4777 
                     /label="PolyA signal"  
     misc_feature    4249..4268 
                     /label="Restriction Enzyme"  
     misc_feature    5053..5725 
                     /label="ColE1"  
     misc_feature    6155..6949 
                     /label="Kan/Neo"  
     misc_feature    6984..7286 
                     /label="SV40 ori"  
     misc_feature    7372..7849 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4053..4059 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4053 
                     /label="SEMA6D"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGGGTCTTC CTGCTTTGTG CCTACATACT GCTGCTGATG GTTTCCCAGT 
     1081 TGAGGGCAGT CAGCTTTCCT GAAGATGATG AACCCCTTAA TACTGTCGAC TATCACTATT 
     1141 CAAGGCAATA TCCGGTTTTT AGAGGACGCC CTTCAGGCAA TGAATCGCAG CACAGGCTGG 
     1201 ACTTTCAGCT GATGTTGAAA ATTCGAGACA CACTTTATAT TGCTGGCAGG GATCAAGTTT 
     1261 ATACAGTAAA CTTAAATGAA ATGCCCAAAA CAGAAGTAAT ACCCAACAAG AAACTGACAT 
     1321 GGCGATCAAG ACAACAGGAT CGAGAAAACT GTGCTATGAA AGGCAAGCAT AAAGATGAAT 
     1381 GCCACAACTT TATCAAAGTA TTTGTTCCAA GAAACGATGA GATGGTTTTT GTTTGTGGTA 
     1441 CCAATGCATT CAATCCCATG TGTAGATACT ACAGGTTGAG TACCTTAGAA TATGATGGGG 
     1501 AAGAAATTAG TGGCCTGGCA AGATGCCCAT TTGATGCCAG ACAAACCAAT GTTGCCCTCT 
     1561 TTGCTGATGG GAAGCTGTAT TCTGCCACAG TGGCTGACTT CTTGGCCAGC GATGCCGTTA 
     1621 TTTATCGAAG CATGGGTGAT GGATCTGCCC TTCGCACAAT AAAATATGAT TCCAAATGGA 
     1681 TAAAAGAGCC ACACTTTCTT CATGCCATAG AATATGGAAA CTATGTCTAT TTCTTCTTTC 
     1741 GAGAAATCGC TGTCGAACAT AATAATTTAG GCAAGGCTGT GTATTCCCGC GTGGCCCGCA 
     1801 TATGTAAAAA CGACATGGGT GGTTCCCAGC GGGTCCTGGA GAAACACTGG ACTTCATTTC 
     1861 TAAAGGCTCG GCTGAACTGT TCTGTCCCTG GAGATTCGTT TTTCTACTTT GATGTTCTGC 
     1921 AGTCTATTAC AGACATAATA CAAATCAATG GCATCCCCAC TGTGGTCGGG GTGTTTACCA 
     1981 CGCAGCTCAA TAGCATCCCT GGTTCTGCTG TCTGTGCATT TAGCATGGAT GACATTGAAA 
     2041 AAGTATTCAA AGGACGGTTT AAGGAACAGA AAACTCCAGA TTCTGTTTGG ACAGCAGTTC 
     2101 CCGAAGACAA AGTGCCAAAG CCAAGGCCTG GCTGTTGTGC AAAACACGGC CTTGCCGAAG 
     2161 CTTATAAAAC CTCCATCGAT TTCCCGGATG AAACTCTGTC ATTCATCAAA TCTCATCCCC 
     2221 TGATGGACTC TGCCGTTCCA CCCATTGCCG ATGAGCCCTG GTTCACAAAG ACTCGGGTCA 
     2281 GGTACAGACT GACGGCCATC TCAGTGGACC ATTCAGCCGG ACCCTACCAG AACTACACAG 
     2341 TCATCTTTGT TGGCTCTGAA GCTGGCATGG TACTTAAAGT TCTGGCAAAG ACCAGTCCTT 
     2401 TCTCTTTGAA CGACAGCGTA TTACTGGAAG AGATTGAAGC CTACAACCAT GCAAAGTGCA 
     2461 GTGCTGAGAA TGAGGAAGAC AAAAAGGTCA TCTCATTACA GTTGGATAAA GATCACCACG 
     2521 CTTTATATGT GGCGTTCTCT AGCTGCATTA TCCGCATCCC CCTCAGTCGC TGTGAGCGTT 
     2581 ATGGATCATG TAAAAAGTCT TGTATTGCAT CTCGTGACCC GTATTGTGGC TGGTTAAGCC 
     2641 AGGGATCCTG TGGTAGAGTG ACCCCAGGGA TGCTGCTGTT AACCGAAGAC TTCTTTGCTT 
     2701 TCCATAACCA CAGTGCTGAA GGATATGAAC AAGACACAGA ATTCGGCAAC ACAGCTCATC 
     2761 TAGGGGACTG CCATGGTGTA CGATGGGAAG TCCAGTCTGG AGAGTCCAAC CAGATGGTCC 
     2821 ACATGAATGT CCTCATCACC TGTGTCTTTG CTGCTTTTGT TTTGGGGGCA TTCATTGCAG 
     2881 GTGTGGCAGT ATACTGCTAT CGAGACATGT TTGTTCGGAA AAACAGAAAG ATCCATAAAG 
     2941 ATGCAGAGTC CGCCCAGTCA TGCACAGACT CCAGTGGAAG TTTTGCCAAA CTGAATGGTC 
     3001 TCTTTGACAG CCCTGTCAAG GAATACCAAC AGAATATTGA TTCTCCTAAA CTGTATAGTA 
     3061 ACCTGCTAAC CAGTCGGAAA GAGCTACCAC CCAATGGAGA TACTAAATCC ATGGTAATGG 
     3121 ACCATCGAGG GCAACCTCCA GAGTTGGCTG CTCTTCCTAC TCCTGAGTCT ACACCCGTGC 
     3181 TTCACCAGAA GACCCTGCAG GCCATGAAGA GCCACTCAGA AAAGGCCCAT GGCCATGGAG 
     3241 CTTCAAGGAA AGAAACCCCT CAGTTTTTTC CGTCTAGTCC GCCACCTCAT TCCCCATTAA 
     3301 GTCATGGGCA TATCCCCAGT GCCATTGTTC TTCCAAATGC TACCCATGAC TACAACACGT 
     3361 CTTTCTCAAA CTCCAATGCT CACAAAGCTG AAAAGAAGCT TCAAAACATT GATCACCCTC 
     3421 TCACAAAGTC ATCCAGTAAG AGAGATCACC GGCGTTCTGT TGATTCCAGA AATACCCTCA 
     3481 ATGATCTCCT GAAGCATCTG AATGACCCAA ATAGTAACCC CAAAGCCATC ATGGGAGACA 
     3541 TCCAGATGGC ACACCAGAAC TTAATGCTGG ATCCCATGGG ATCGATGTCT GAGGTCCCAC 
     3601 CTAAAGTCCC TAACCGGGAG GCATCGCTAT ACTCCCCTCC TTCAACTCTC CCCAGAAATA 
     3661 GCCCAACCAA GCGAGTGGAT GTCCCCACCA CTCCTGGAGT CCCAATGACT TCTCTGGAAA 
     3721 GACAAAGAGG TTATCACAAA AATTCCTCCC AGAGGCACTC TATATCTGCT ATGCCTAAAA 
     3781 ACTTAAACTC ACCAAATGGT GTTTTGTTAT CCAGACAGCC TAGTATGAAC CGTGGAGGAT 
     3841 ATATGCCCAC CCCCACTGGG GCGAAGGTGG ACTATATTCA GGGAACACCA GTGAGTGTTC 
     3901 ATCTGCAGCC TTCCCTCTCC AGACAGAGCA GCTACACCAG TAATGGCACT CTTCCTAGGA 
     3961 CGGGACTAAA GAGGACGCCG TCCTTAAAAC CTGACGTGCC ACCAAAGCCT TCCTTTGTTC 
     4021 CTCAAACCCC ATCTGTCAGA CCACTGAACA AATACACATA CACGCGTACG CGGCCGCTCG 
     4081 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4141 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4201 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4261 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4321 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4381 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4441 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4501 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4561 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4621 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4681 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4741 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4801 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4861 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     4921 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     4981 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5041 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5101 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5161 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5221 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5281 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5341 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5401 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5461 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5521 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5581 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5641 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5701 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5761 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     5821 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     5881 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     5941 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6001 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6061 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6121 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6181 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6241 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6301 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6361 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6421 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6481 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6541 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6601 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6661 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6721 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6781 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6841 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     6901 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     6961 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7021 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7081 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7141 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7201 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7261 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7321 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7381 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7441 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7501 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7561 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7621 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7681 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7741 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7801 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7861 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
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