LOCUS       RC233174                7632 bp    DNA     circular  SYN 
DEFINITION  NRP1 (Myc-DDK tagged) - Homo sapiens neuropilin 1 (NRP1), transcript variant 4
ACCESSION   NM_001244972
VERSION     NM_001244972.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3789..3880 
                     /label="Myc-DDK"  
     misc_feature    3910..4495 
                     /label="PolyA signal"  
     misc_feature    3967..3986 
                     /label="Restriction Enzyme"  
     misc_feature    4771..5443 
                     /label="ColE1"  
     misc_feature    5873..6667 
                     /label="Kan/Neo"  
     misc_feature    6702..7004 
                     /label="SV40 ori"  
     misc_feature    7090..7567 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3771..3777 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3771 
                     /label="NRP1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGAGGGGG CTGCCGCTCC TCTGCGCCGT GCTCGCCCTC GTCCTCGCCC 
     1081 CGGCCGGCGC TTTTCGCAAC GATAAATGTG GCGATACTAT AAAAATTGAA AGCCCCGGGT 
     1141 ACCTTACATC TCCTGGTTAT CCTCATTCTT ATCACCCAAG TGAAAAATGC GAATGGCTGA 
     1201 TTCAGGCTCC GGACCCATAC CAGAGAATTA TGATCAACTT CAACCCTCAC TTCGATTTGG 
     1261 AGGACAGAGA CTGCAAGTAT GACTACGTGG AAGTCTTCGA TGGAGAAAAT GAAAATGGAC 
     1321 ATTTTAGGGG AAAGTTCTGT GGAAAGATAG CCCCTCCTCC TGTTGTGTCT TCAGGGCCAT 
     1381 TTCTTTTTAT CAAATTTGTC TCTGACTACG AAACACATGG TGCAGGATTT TCCATACGTT 
     1441 ATGAAATTTT CAAGAGAGGT CCTGAATGTT CCCAGAACTA CACAACACCT AGTGGAGTGA 
     1501 TAAAGTCCCC CGGATTCCCT GAAAAATATC CCAACAGCCT TGAATGCACT TATATTGTCT 
     1561 TTGCGCCAAA GATGTCAGAG ATTATCCTGG AATTTGAAAG CTTTGACCTG GAGCCTGACT 
     1621 CAAATCCTCC AGGGGGGATG TTCTGTCGCT ACGACCGGCT AGAAATCTGG GATGGATTCC 
     1681 CTGATGTTGG CCCTCACATT GGGCGTTACT GTGGACAGAA AACACCAGGT CGAATCCGAT 
     1741 CCTCATCGGG CATTCTCTCC ATGGTTTTTT ACACCGACAG CGCGATAGCA AAAGAAGGTT 
     1801 TCTCAGCAAA CTACAGTGTC TTGCAGAGCA GTGTCTCAGA AGATTTCAAA TGTATGGAAG 
     1861 CTCTGGGCAT GGAATCAGGA GAAATTCATT CTGACCAGAT CACAGCTTCT TCCCAGTATA 
     1921 GCACCAACTG GTCTGCAGAG CGCTCCCGCC TGAACTACCC TGAGAATGGG TGGACTCCCG 
     1981 GAGAGGATTC CTACCGAGAG TGGATACAGG TAGACTTGGG CCTTCTGCGC TTTGTCACGG 
     2041 CTGTCGGGAC ACAGGGCGCC ATTTCAAAAG AAACCAAGAA GAAATATTAT GTCAAGACTT 
     2101 ACAAGATCGA CGTTAGCTCC AACGGGGAAG ACTGGATCAC CATAAAAGAA GGAAACAAAC 
     2161 CTGTTCTCTT TCAGGGAAAC ACCAACCCCA CAGATGTTGT GGTTGCAGTA TTCCCCAAAC 
     2221 CACTGATAAC TCGATTTGTC CGAATCAAGC CTGCAACTTG GGAAACTGGC ATATCTATGA 
     2281 GATTTGAAGT ATACGGTTGC AAGATAACAG ATTATCCTTG CTCTGGAATG TTGGGTATGG 
     2341 TGTCTGGACT TATTTCTGAC TCCCAGATCA CATCATCCAA CCAAGGGGAC AGAAACTGGA 
     2401 TGCCTGAAAA CATCCGCCTG GTAACCAGTC GCTCTGGCTG GGCACTTCCA CCCGCACCTC 
     2461 ATTCCTACAT CAATGAGTGG CTCCAAATAG ACCTGGGGGA GGAGAAGATC GTGAGGGGCA 
     2521 TCATCATTCA GGGTGGGAAG CACCGAGAGA ACAAGGTGTT CATGAGGAAG TTCAAGATCG 
     2581 GGTACAGCAA CAACGGCTCG GACTGGAAGA TGATCATGGA TGACAGCAAA CGCAAGGCGA 
     2641 AGTCTTTTGA GGGCAACAAC AACTATGATA CACCTGAGCT GCGGACTTTT CCAGCTCTCT 
     2701 CCACGCGATT CATCAGGATC TACCCCGAGA GAGCCACTCA TGGCGGACTG GGGCTCAGAA 
     2761 TGGAGCTGCT GGGCTGTGAA GTGGAAGCCC CTACAGCTGG ACCGACCACT CCCAACGGGA 
     2821 ACTTGGTGGA TGAATGTGAT GACGACCAGG CCAACTGCCA CAGTGGAACA GGTGGCACCA 
     2881 CTGTGCTGGC CACAGAAAAG CCCACGGTCA TAGACAGCAC CATACAATCA GAGTTTCCAA 
     2941 CATATGGTTT TAACTGTGAA TTTGGCTGGG GCTCTCACAA GACCTTCTGC CACTGGGAAC 
     3001 ATGACAATCA CGTGCAGCTC AAGTGGAGTG TGTTGACCAG CAAGACGGGA CCCATTCAGG 
     3061 ATCACACAGC AGGAGATGGC AACTTCATCT ATTCCCAAGC TGACGAAAAT CAGAAGGGCA 
     3121 AAGTGGCTCG CCTGGTGAGC CCTGTGGTTT ATTCCCAGAA CTCTGCCCAC TGCATGACCT 
     3181 TCTGGTATCA CATGTCTGGG TCCCACGTCG GCACACTCAG GGTCAAACTG CGCTACCAGA 
     3241 AGCCAGAGGA GTACGATCAG CTGGTCTGGA TGGCCATTGG ACACCAAGGT GACCACTGGA 
     3301 AGGAAGGGCG TGTCTTGCTC CACAAGTCTC TGAAACTTTA TCAGGTGATT TTCGAGGGCG 
     3361 AAATCGGAAA AGGAAACCTT GGTGGGATTG CTGTGGATGA CATTAGTATT AATAACCACA 
     3421 TTTCACAAGA AGATTGTGCA AAACCAGCAG ACCTGGATAA AAAGAACCCA GAAATTAAAA 
     3481 TTGATGAAAC AGGGAGCACG CCAGGATACG AAGGTGAAGG AGAAGGTGAC AAGAACATCT 
     3541 CCAGGAAGCC AGGCAATGTG TTGAAGACCT TAGACCCCAT CCTCATCACC ATCATAGCCA 
     3601 TGAGTGCCCT GGGGGTCCTC CTGGGGGCTG TCTGTGGGGT CGTGCTGTAC TGTGCCTGTT 
     3661 GGCATAATGG GATGTCAGAA AGAAACTTGT CTGCCCTGGA GAACTATAAC TTTGAACTTG 
     3721 TGGATGGTGT GAAGTTGAAA AAAGACAAAC TGAATACACA GAGTACTTAT TCGGAGGCAA 
     3781 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     3841 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     3901 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     3961 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4021 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4081 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4141 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4201 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4261 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4321 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     4381 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     4441 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     4501 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     4561 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     4621 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     4681 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     4741 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     4801 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     4861 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     4921 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     4981 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5041 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     5101 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     5161 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     5221 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     5281 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     5341 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     5401 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     5461 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     5521 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     5581 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     5641 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     5701 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     5761 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     5821 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     5881 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     5941 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6001 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6061 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     6121 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     6181 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     6241 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     6301 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     6361 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     6421 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     6481 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     6541 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     6601 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     6661 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     6721 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     6781 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     6841 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     6901 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     6961 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7021 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7081 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     7141 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     7201 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     7261 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     7321 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     7381 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     7441 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     7501 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     7561 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     7621 AACGCGAATT TT
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