LOCUS       RC233169                7614 bp    DNA     circular  SYN 
DEFINITION  NEDD4L (Myc-DDK tagged) - Homo sapiens neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase (NEDD4L), transcript variant i
ACCESSION   NM_001243960
VERSION     NM_001243960.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3771..3862 
                     /label="Myc-DDK"  
     misc_feature    3892..4477 
                     /label="PolyA signal"  
     misc_feature    3949..3968 
                     /label="Restriction Enzyme"  
     misc_feature    4753..5425 
                     /label="ColE1"  
     misc_feature    5855..6649 
                     /label="Kan/Neo"  
     misc_feature    6684..6986 
                     /label="SV40 ori"  
     misc_feature    7072..7549 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3753..3759 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3753 
                     /label="NEDD4L"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGACCGGG CTCGGGGAGC CGGTCTATGG ACTTTCCGAA GACGAGGGAG 
     1081 AGTCCCGTAT TCTCAGAGTA AAAGTTGTTT CTGGAATTGA TCTCGCCAAA AAGGACATCT 
     1141 TTGGAGCCAG TGATCCGTAT GTGAAACTTT CATTGTACGT AGCGGATGAG AATAGAGAAC 
     1201 TTGCTTTGGT CCAGACAAAA ACAATTAAAA AGACACTGAA CCCAAAATGG AATGAAGAAT 
     1261 TTTATTTCAG GGTAAACCCA TCTAATCACA GACTCCTATT TGAAGTATTT GACGAAAATA 
     1321 GACTGACACG AGACGACTTC CTGGGCCAGG TGGACGTGCC CCTTAGTCAC CTTCCGACAG 
     1381 AAGATCCAAC CATGGAGCGA CCCTATACAT TTAAGGACTT TCTCCTCAGA CCAAGAAGTC 
     1441 ATAAGTCTCG AGTTAAGGGA TTTTTGCGAT TGAAAATGGC CTATATGCCA AAAAATGGAG 
     1501 GTCAAGATGA AGAAAACAGT GACCAGAGGG ATGACATGGA GCATGGATGG GAAGTTGTTG 
     1561 ACTCAAATGA CTCGGCTTCT CAGCACCAAG AGGAACTTCC TCCTCCTCCT CTGCCTCCCG 
     1621 GGTGGGAAGA AAAAGTGGAC AATTTAGGCC GAACTTACTA TGTCAACCAC AACAACCGGA 
     1681 CCACTCAGTG GCACAGACCA AGCCTGATGG ACGTGTCCTC GGAGTCGGAC AATAACATCA 
     1741 GACAGATCAA CCAGGAGGCA GCACACCGGC GCTTCCGCTC CCGCAGGCAC ATCAGCGAAG 
     1801 ACTTGGAGCC CGAGCCCTCG GAGGGCGGGG ATGTCCCCGA GCCTTGGGAG ACCATTTCAG 
     1861 AGGAAGTGAA TATCGCTGGA GACTCTCTCG GTCTGGCTCT GCCCCCACCA CCGGCCTCCC 
     1921 CAGGATCTCG GACCAGCCCT CAGGAGCTGT CAGAGGAACT AAGCAGAAGG CTTCAGATCA 
     1981 CTCCAGACTC CAATGGGGAA CAGTTCAGCT CTTTGATTCA AAGAGAACCC TCCTCAAGGT 
     2041 TGAGGTCATG CAGTGTCACC GACGCAGTTG CAGAACAGGG CCATCTACCA CCGCTTGCAG 
     2101 AAGATGGTGC GTCCGGATCA GCCACAAACA GTAACAACCA TCTAATCGAG CCTCAGATCC 
     2161 GCCGGCCTCG TAGCCTCAGC TCGCCAACAG TAACTTTATC TGCCCCGCTG GAGGGTGCCA 
     2221 AGGACTCACC CGTACGTCGG GCTGTGAAAG ACACCCTTTC CAACCCACAG TCCCCACAGC 
     2281 CATCACCTTA CAACTCCCCC AAACCACAAC ACAAAGTCAC ACAGAGCTTC TTGCCACCCG 
     2341 GCTGGGAAAT GAGGATAGCG CCAAACGGCC GGCCCTTCTT CATTGATCAT AACACAAAGA 
     2401 CTACAACCTG GGAAGATCCA CGTTTGAAAT TTCCAGTACA TATGCGGTCA AAGACATCTT 
     2461 TAAACCCCAA TGACCTTGGC CCCCTTCCTC CTGGCTGGGA AGAAAGAATT CACTTGGATG 
     2521 GCCGAACGTT TTATATTGAT CATAATAGCA AAATTACTCA GTGGGAAGAC CCAAGACTGC 
     2581 AGAACCCAGC TATTACTGGT CCGGCTGTCC CTTACTCCAG AGAATTTAAG CAGAAATATG 
     2641 ACTACTTCAG GAAGAAATTA AAGAAACCTG CTGATATCCC CAATAGGTTT GAAATGAAAC 
     2701 TTCACAGAAA TAACATATTT GAAGAGTCCT ATCGGAGAAT TATGTCCGTG AAAAGACCAG 
     2761 ATGTCCTAAA AGCTAGACTG TGGATTGAGT TTGAATCAGA GAAAGGTCTT GACTATGGGG 
     2821 GTGTGGCCAG AGAATGGTTC TTCTTACTGT CCAAAGAGAT GTTCAACCCC TACTACGGCC 
     2881 TCTTTGAGTA CTCTGCCACG GACAACTACA CCCTTCAGAT CAACCCTAAT TCAGGCCTCT 
     2941 GTAATGAGGA TCATTTGTCC TACTTCACTT TTATTGGAAG AGTTGCTGGT CTGGCCGTAT 
     3001 TTCATGGGAA GCTCTTAGAT GGTTTCTTCA TTAGACCATT TTACAAGATG ATGTTGGGAA 
     3061 AGCAGATAAC CCTGAATGAC ATGGAATCTG TGGATAGTGA ATATTACAAC TCTTTGAAAT 
     3121 GGATCCTGGA GAATGACCCT ACTGAGCTGG ACCTCATGTT CTGCATAGAC GAAGAAAACT 
     3181 TTGGACAGAC ATATCAAGTG GATTTGAAGC CCAATGGGTC AGAAATAATG GTCACAAATG 
     3241 AAAACAAAAG GGAATATATC GACTTAGTCA TCCAGTGGAG ATTTGTGAAC AGGGTCCAGA 
     3301 AGCAGATGAA CGCCTTCTTG GAGGGATTCA CAGAACTACT TCCTATTGAT TTGATTAAAA 
     3361 TTTTTGATGA AAATGAGCTG GAGTTGCTCA TGTGCGGCCT CGGTGATGTG GATGTGAATG 
     3421 ACTGGAGACA GCATTCTATT TACAAGAACG GCTACTGCCC AAACCACCCC GTCATTCAGT 
     3481 GGTTCTGGAA GGCTGTGCTA CTCATGGACG CCGAAAAGCG TATCCGGTTA CTGCAGTTTG 
     3541 TCACAGGGAC ATCGCGAGTA CCTATGAATG GATTTGCCGA ACTTTATGGT TCCAATGGTC 
     3601 CTCAGCTGTT TACAATAGAG CAATGGGGCA GTCCTGAGAA ACTGCCCAGA GCTCACACAT 
     3661 GCTTTAATCG CCTTGACTTA CCTCCATATG AAACCTTTGA AGATTTACGA GAGAAACTTC 
     3721 TCATGGCCGT GGAAAATGCT CAAGGATTTG AAGGGGTGGA TACGCGTACG CGGCCGCTCG 
     3781 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     3841 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     3901 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     3961 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4021 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4081 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4141 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4201 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4261 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4321 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4381 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4441 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4501 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4561 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     4621 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     4681 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     4741 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     4801 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     4861 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     4921 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     4981 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5041 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5101 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5161 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5221 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5281 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5341 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5401 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5461 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     5521 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     5581 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     5641 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     5701 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     5761 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     5821 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     5881 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     5941 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6001 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6061 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6121 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6181 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6241 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6301 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6361 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6421 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6481 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6541 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     6601 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     6661 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     6721 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     6781 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     6841 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6901 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     6961 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7021 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7081 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7141 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7201 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7261 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7321 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7381 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7441 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7501 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7561 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
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