LOCUS       RC233114                7116 bp    DNA     circular  SYN 
DEFINITION  CUL2 (Myc-DDK tagged) - Homo sapiens cullin 2 (CUL2), transcript variant 4
ACCESSION   NM_001198777
VERSION     NM_001198777.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3273..3364 
                     /label="Myc-DDK"  
     misc_feature    3394..3979 
                     /label="PolyA signal"  
     misc_feature    3451..3470 
                     /label="Restriction Enzyme"  
     misc_feature    4255..4927 
                     /label="ColE1"  
     misc_feature    5357..6151 
                     /label="Kan/Neo"  
     misc_feature    6186..6488 
                     /label="SV40 ori"  
     misc_feature    6574..7051 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3255..3261 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3255 
                     /label="CUL2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTTTGAAA CCAAGAGTAG TAGATTTTGA TGAAACATGG AACAAACTTT 
     1081 TGACGACAAT AAAAGCCGTG GTCATGTTGG AATACGTCGA AAGAGCAACA TGGAATGACC 
     1141 GTTTCTCAGA TATCTATGCT TTATGTGTGG CCTATCCTGA ACCCCTTGGA GAAAGACTTT 
     1201 ATACAGAAAC TAAGATTTTT TTGGAAAATC ATGTTCGGCA TTTGCATAAG AGAGTTTTGG 
     1261 AGTCAGAAGA ACAAGTACTT GTTATGTATC ATAGGTACTG GGAAGAATAC AGCAAGGGTG 
     1321 CAGACTATAT GGACTGCTTA TATAGGTATC TCAACACCCA GTTTATTAAA AAGAATAAAT 
     1381 TAACAGAAGC GGACCTTCAG TATGGCTATG GTGGTGTAGA TATGAATGAA CCACTTATGG 
     1441 AAATAGGAGA GCTAGCATTG GATATGTGGA GGAAATTGAT GGTTGAACCA CTTCAGGCCA 
     1501 TCCTTATCCG AATGCTGCTC CGAGAAATCA AAAATGATCG TGGTGGAGAA GACCCAAACC 
     1561 AGAAAGTAAT CCATGGGGTT ATTAACTCCT TTGTTCATGT TGAACAGTAT AAGAAAAAAT 
     1621 TCCCCTTAAA GTTTTATCAG GAAATTTTTG AGTCTCCCTT TCTGACTGAA ACAGGAGAGT 
     1681 ATTACAAACA AGAAGCTTCA AATTTATTAC AAGAATCAAA CTGCTCACAG TATATGGAAA 
     1741 AGGTTCTAGG TAGATTAAAA GATGAAGAAA TTCGATGTCG AAAATACCTA CATCCAAGTT 
     1801 CATATACTAA GGTGATTCAT GAATGTCAAC AACGAATGGT AGCAGACCAC TTACAGTTTT 
     1861 TACATGCAGA ATGTCATAAT ATAATTCGAC AAGAGAAAAA AAATGACATG GCAAATATGT 
     1921 ACGTCTTACT CCGTGCTGTG TCCACTGGTT TACCTCATAT GATTCAGGAG CTGCAAAACC 
     1981 ACATCCATGA TGAGGGCCTT CGAGCAACCA GCAACCTTAC TCAGGAAAAC ATGCCAACAC 
     2041 TATTTGTGGA GTCAGTTTTG GAAGTGCATG GTAAATTTGT TCAGCTTATC AACACTGTTT 
     2101 TGAATGGTGA TCAGCATTTT ATGAGTGCGT TGGATAAGGC CCTTACGTCA GTTGTAAATT 
     2161 ACAGAGAACC TAAGTCTGTT TGCAAAGCAC CTGAACTGCT TGCTAAGTAC TGTGACAACT 
     2221 TACTGAAGAA GTCAGCGAAA GGGATGACAG AGAATGAAGT GGAAGACAGG CTCACGAGCT 
     2281 TCATCACAGT GTTCAAATAC ATTGATGACA AGGACGTCTT TCAAAAGTTC TACGCAAGAA 
     2341 TGCTGGCAAA ACGTTTAATT CATGGGTTAT CCATGTCTAT GGACTCTGAA GAAGCCATGA 
     2401 TCAACAAATT AAAGCAAGCC TGTGGTTATG AGTTTACCAG CAAGCTACAT CGGATGTATA 
     2461 CAGATATGAG TGTCAGCGCT GATCTCAACA ATAAGTTCAA CAATTTTATC AAAAACCAAG 
     2521 ACACAGTAAT AGATTTGGGA ATTAGTTTTC AAATATATGT TCTACAGGCT GGTGCGTGGC 
     2581 CTCTTACTCA GGCTCCTTCA TCTACGTTTG CAATTCCCCA GGAATTAGAA AAAAGTGTAC 
     2641 AGATGTTTGA ATTATTTTAT AGCCAACATT TCAGTGGAAG GAAACTTACA TGGTTACATT 
     2701 ATCTGTGTAC AGGTGAAGTT AAAATGAACT ATTTGGGCAA ACCATATGTA GCCATGGTTA 
     2761 CAACATACCA AATGGCAGTT CTTCTTGCCT TTAACAACAG TGAAACTGTC AGTTATAAAG 
     2821 AGCTTCAGGA CAGCACTCAG ATGAATGAAA AGGAACTGAC AAAAACAATC AAATCATTAC 
     2881 TTGATGTGAA AATGATTAAC CATGATTCAG AAAAGGAAGA TATTGATGCA GAATCTTCGT 
     2941 TTTCATTAAA TATGAACTTT AGCAGTAAAA GAACAAAATT TAAAATTACT ACATCAATGC 
     3001 AGAAAGACAC ACCACAAGAA ATGGAGCAGA CTAGAAGTGC AGTTGATGAG GACCGGAAAA 
     3061 TGTATCTCCA AGCTGCTATA GTTCGTATCA TGAAAGCACG AAAAGTGCTT CGGCACAATG 
     3121 CCCTTATTCA AGAGGTGATT AGCCAGTCAA GAGCTAGGTT TAATCCCAGT ATCAGCATGA 
     3181 TTAAGAAGTG TATTGAAGTT CTGATAGACA AACAATACAT AGAACGCAGC CAGGCGTCGG 
     3241 CAGATGAATA CAGCTACGTC GCGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     3301 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     3361 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     3421 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     3481 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     3541 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     3601 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     3661 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     3721 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     3781 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     3841 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     3901 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     3961 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     4021 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     4081 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     4141 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     4201 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     4261 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     4321 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     4381 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     4441 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     4501 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     4561 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     4621 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     4681 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     4741 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     4801 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     4861 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     4921 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     4981 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     5041 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     5101 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     5161 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     5221 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     5281 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     5341 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     5401 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     5461 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     5521 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     5581 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     5641 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     5701 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     5761 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     5821 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     5881 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     5941 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     6001 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     6061 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     6121 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     6181 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     6241 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     6301 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     6361 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6421 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6481 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     6541 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     6601 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     6661 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     6721 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     6781 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     6841 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     6901 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     6961 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     7021 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     7081 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//