LOCUS       RC233107                7086 bp    DNA     circular  SYN 
DEFINITION  STON1 (Myc-DDK tagged) - Homo sapiens stonin 1 (STON1), transcript variant 1
ACCESSION   NM_001198595
VERSION     NM_001198595.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3243..3334 
                     /label="Myc-DDK"  
     misc_feature    3364..3949 
                     /label="PolyA signal"  
     misc_feature    3421..3440 
                     /label="Restriction Enzyme"  
     misc_feature    4225..4897 
                     /label="ColE1"  
     misc_feature    5327..6121 
                     /label="Kan/Neo"  
     misc_feature    6156..6458 
                     /label="SV40 ori"  
     misc_feature    6544..7021 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3225..3231 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3225 
                     /label="STON1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGCTCCACA AATCCAGGCA AATGGGTCAC CTTTGATGAT GATCCTGCTG 
     1081 TTCAATCTTC TCAAAAGTCA AAGAATTTTC CTCTGGAGAA TCAAGGTGTC TGTAGACCAA 
     1141 ATGGACTGAA GCTGAACCTT CCTGGCCTCA GGGAATTTCC CAGTGGATCT TCCTCCACCA 
     1201 GCAGCACTCC TCTCTCCTCC CCCATTGTAG ATTTTTATTT CAGTCCAGGA CCTCCAAGTA 
     1261 ACTCTCCTCT TTCTACACCT ACCAAAGACT TCCCAGGTTT TCCTGGCATC CCCAAAGCAG 
     1321 GGACTCATGT GCTTTATCCT ATTCCAGAAT CATCTTCAGA CAGCCCACTC GCAATATCAG 
     1381 GAGGAGAATC TTCCTTACTG CCTACCAGAC CAACATGTTT ATCCCATGCC TTGTTACCCA 
     1441 GTGACCACTC ATGTACACAT CCAACTCCCA AAGTAGGTCT TCCAGATGAA GTTAATCCTC 
     1501 AACAGGCTGA AAGCCTAGGA TTCCAAAGTG ATGATCTCCC CCAGTTTCAG TATTTTCGAG 
     1561 AGGACTGTGC TTTTTCAAGT CCATTTTGGA AAGATGAAGG CAGTGATTCC CATTTCACCC 
     1621 TTGACCCACC AGGAAGCAAA AAGATGTTCT CATCAAGAAA CAAGGAGATG CCTATTGACC 
     1681 AAAAAAGCCT AAATAAGTGT TCACTCAACT ATATCTGTGA GAAGCTTGAA CATCTCCAGT 
     1741 CAGCTGAGAA CCAAGACTCA CTTAGAAGTT TGTCTATGCA CTGTCTATGT GCTGAAGAAA 
     1801 ATGCCTCTTC CTTTGTCCCC CACACACTCT TCAGGAGTCA GCCAAAATCC GGATGGTCTT 
     1861 TCATGCTGAG AATTCCTGAG AAGAAGAATA TGATGTCTTC CCGGCAATGG GGACCAATTT 
     1921 TTCTGAAAGT TTTGCCTGGA GGAATTTTGC AGATGTATTA TGAACAGGGA TTAGAAAAAC 
     1981 CATTTAAAGA GATACAGCTT GATCCATATT GTAGGCTTTC TGAACCCAAG GTTGAGAACT 
     2041 TCAGTGTAGC AGGAAAAATC CACACTGTGA AGATTGAACA TGTGTCTTAC ACAGAAAAAA 
     2101 GGAAATACCA TTCTAAGACA GAAGTAGTTC ATGAACCTGA CATAGAGCAG ATGCTGAAGT 
     2161 TGGGGTCCAC ATCGTACCAT GACTTCCTTG ACTTTCTGAC TACTGTGGAG GAGGAGCTGA 
     2221 TGAAGTTGCC AGCTGTTTCA AAACCAAAAA AGAACTACGA GGAGCAAGAA ATTTCCTTGG 
     2281 AAATTGTGGA CAACTTTTGG GGTAAAGTCA CAAAAGAAGG AAAATTTGTT GAAAGTGCTG 
     2341 TGATAACTCA AATTTATTGC CTCTGCTTTG TGAATGGGAA CCTGGAATGC TTTTTAACCT 
     2401 TGAATGACCT TGAGTTGCCG AAGCGAGATG AATCCTATTA TGAGAAGGAC TCAGAAAAAA 
     2461 AGGGGATTGA TATTCTTGAC TACCATTTTC ATAAGTGTGT GAATGTACAA GAATTTGAGC 
     2521 AATCAAGAAT CATTAAGTTT GTACCTCTGG ATGCCTGCCG GTTTGAGCTG ATGCGTTTCA 
     2581 AGACTTTGTA TAATGGGGAT AATCTTCCCT TTTCCTTGAA GTCTGTAGTG GTTGTCCAGG 
     2641 GAGCATACGT GGAACTTCAG GCTTTTGTCA ACATGGCCTC ATTGGCGCAG AGGTCATCCT 
     2701 ATGCTGGTTC CTTAAGGTCC TGTGACAATA TAAGGATACA CTTTCCTGTC CCATCGCAGT 
     2761 GGATCAAGGC CCTTTGGACC ATGAACCTCC AGAGGCAGAA GTCTCTGAAA GCTAAAATGA 
     2821 ACCGCCGAGC ATGTCTGGGG AGTTTACAGG AACTTGAATC TGAACCTGTC ATTCAAGTCA 
     2881 CTGTGGGGTC AGCAAAATAT GAGAGTGCCT ACCAGGCAGT GGTATGGAAG ATAGATCGGC 
     2941 TTCCAGACAA AAATTCAAGT CTAGATCATC CCCATTGTCT GTCATACAAA TTAGAGCTTG 
     3001 GATCAGACCA AGAAATTCCC TCTGATTGGT ATCCATTTGC TACTGTTCAG TTTTCCGTGC 
     3061 CTGACACCTG TGCCTCAAGG ACAGAGGTCA GGTCTCTGGG AGTGGAGAGT GATGTCCAGC 
     3121 CACAGAAACA TGTTCAGCAG CGAGCTTGCT ACAACATCCA GGTTGAAATA GAAAAGAAGT 
     3181 GGATTAAAAT CGATGGAGAA GACCCAGATA AAATTGGTGA CTGCATAACT CAGACGCGTA 
     3241 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     3301 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     3361 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     3421 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     3481 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     3541 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     3601 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     3661 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     3721 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     3781 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     3841 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     3901 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     3961 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     4021 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     4081 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     4141 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     4201 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     4261 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     4321 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     4381 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     4441 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     4501 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     4561 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     4621 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     4681 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     4741 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     4801 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     4861 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     4921 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     4981 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     5041 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     5101 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     5161 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     5221 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     5281 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     5341 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     5401 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     5461 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     5521 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     5581 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     5641 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     5701 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     5761 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     5821 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     5881 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     5941 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     6001 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     6061 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     6121 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     6181 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     6241 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     6301 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     6361 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     6421 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6481 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     6541 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     6601 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     6661 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     6721 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     6781 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     6841 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     6901 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     6961 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     7021 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     7081 AATTTT
//