LOCUS RC233104 7059 bp DNA circular SYN DEFINITION HLCS (Myc-DDK tagged) - Homo sapiens holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS), transcript variant 2 ACCESSION NM_001242785 VERSION null SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 3216..3307 /label="Myc-DDK" misc_feature 3337..3922 /label="PolyA signal" misc_feature 3394..3413 /label="Restriction Enzyme" misc_feature 4198..4870 /label="ColE1" misc_feature 5300..6094 /label="Kan/Neo" misc_feature 6129..6431 /label="SV40 ori" misc_feature 6517..6994 /label="f1 ori" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 3198..3204 /label="Restriction Enzyme" misc_feature 1020..3198 /label="HLCS" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GGAAGATAGA CTCCACATGG ATAATGGACT GGTACCCCAA AAGATTGTGT 1081 CGGTGCACTT GCAGGACTCC ACTCTGAAGG AAGTTAAGGA TCAGGTCTCA AACAAGCAAG 1141 CCCAGATCCT AGAGCCGAAG CCTGAACCTT CTCTTGAGAT TAAGCCTGAG CAGGACGGTA 1201 TGGAGCATGT TGGCAGAGAT GACCCAAAGG CTCTTGGTGA AGAACCCAAA CAAAGGAGAG 1261 GCAGTGCCTC TGGGAGTGAG CCTGCTGGGG ACAGTGACAG GGGAGGGGGC CCCGTTGAGC 1321 ATTATCACCT CCATCTGTCT AGTTGCCACG AGTGTCTGGA ACTTGAGAAC AGCACCATTG 1381 AGTCAGTCAA GTTTGCGTCT GCCGAGAACA TTCCAGACCT TCCCTACGAT TATAGCAGCA 1441 GTTTGGAGAG TGTTGCTGAT GAGACCTCCC CCGAAAGAGA AGGGAGGAGA GTCAACCTCA 1501 CGGGAAAGGC ACCCAACATC CTCCTCTATG TGGGCTCCGA CTCCCAGGAA GCCCTCGGCC 1561 GGTTCCACGA GGTCCGGTCT GTGCTGGCCG ACTGTGTGGA CATTGACAGT TATATTCTCT 1621 ACCACCTGCT GGAGGACAGT GCTCTCAGAG ACCCGTGGAC GGACAACTGT CTGCTGTTGG 1681 TCATTGCTAC CAGGGAGTCC ATTCCCGAAG ACCTGTACCA GAAGTTCATG GCCTATCTTT 1741 CTCAGGGAGG GAAGGTGTTG GGCCTGTCTT CATCCTTCAC CTTTGGTGGC TTTCAGGTGA 1801 CAAGCAAGGG TGCACTGCAC AAGACAGTCC AGAACTTGGT TTTCTCCAAG GCTGACCAGA 1861 GTGAGGTGAA GCTCAGCGTC TTGAGCAGTG GCTGCAGGTA CCAGGAAGGC CCCGTCCGGC 1921 TCAGCCCCGG CAGGCTCCAG GGCCACCTGG AGAATGAGGA CAAGGACAGG ATGATTGTGC 1981 ATGTGCCTTT TGGAACTCGC GGGGGAGAAG CTGTTCTTTG CCAGGTGCAC TTAGAACTAC 2041 CTCCCAGCTC CAACATAGTG CAAACTCCAG AAGATTTTAA CTTGCTCAAG TCAAGCAATT 2101 TTAGAAGATA CGAAGTCCTT AGAGAGATTC TGACAACCCT TGGCCTCAGC TGTGACATGA 2161 AACAAGTTCC TGCCTTAACT CCTCTTTACT TGCTGTCAGC TGCGGAGGAA ATCAGGGATC 2221 CTCTTATGCA GTGGCTTGGG AAACATGTGG ACTCCGAGGG AGAAATAAAA TCCGGCCAGC 2281 TCTCTCTTAG ATTTGTTTCA TCCTACGTGT CTGAAGTAGA AATAACCCCA TCTTGTATAC 2341 CTGTGGTGAC CAACATGGAG GCCTTCTCAT CAGAACATTT CAACTTAGAG ATCTATCGCC 2401 AAAATCTGCA GACCAAGCAG TTGGGGAAAG TAATTTTGTT TGCCGAAGTG ACCCCCACAA 2461 CGATGCGTCT CCTGGATGGG CTGATGTTTC AGACACCGCA GGAAATGGGC TTAATAGTGA 2521 TCGCGGCCCG GCAGACCGAG GGCAAAGGAC GGGGAGGGAA TGTGTGGCTG AGCCCTGTGG 2581 GATGTGCTCT TTCTACTCTG CTCATCTCCA TTCCACTGAG ATCCCAGCTG GGACAGAGGA 2641 TCCCGTTTGT CCAGCATCTG ATGTCCGTGG CTGTCGTGGA AGCAGTGAGG TCCATTCCCG 2701 AGTATCAGGA TATCAACTTA CGAGTGAAGT GGCCCAACGA TATTTATTAC AGTGACCTCA 2761 TGAAGATCGG CGGAGTTCTG GTTAACTCAA CACTCATGGG AGAAACATTT TATATACTTA 2821 TTGGCTGTGG ATTTAATGTG ACTAACAGTA ACCCTACCAT CTGCATCAAC GACCTCATCA 2881 CAGAATACAA TAAACAACAC AAGGCAGAAC TGAAGCCCTT AAGAGCCGAT TATCTCATCG 2941 CCAGAGTCGT GACTGTGCTG GAGAAACTGA TCAAAGAGTT TCAGGACAAA GGGCCCAACA 3001 GCGTCCTTCC CCTTTATTAC CGATACTGGG TCCACAGTGG TCAGCAAGTC CATCTGGGCA 3061 GCGCAGAGGG ACCAAAGGTG TCCATCGTTG GCCTGGACGA TTCTGGCTTC CTCCAGGTTC 3121 ACCAGGAGGG CGGCGAGGTT GTGACTGTGC ACCCGGACGG CAACTCCTTC GACATGCTGA 3181 GAAACCTCAT CCTCCCCAAA CGGCGGACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 3241 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 3301 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 3361 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 3421 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 3481 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 3541 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 3601 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 3661 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 3721 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 3781 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 3841 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 3901 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 3961 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 4021 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 4081 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 4141 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 4201 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 4261 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 4321 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 4381 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 4441 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 4501 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 4561 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 4621 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 4681 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 4741 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 4801 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 4861 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 4921 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 4981 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 5041 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 5101 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 5161 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 5221 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 5281 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 5341 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 5401 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 5461 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 5521 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 5581 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 5641 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 5701 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 5761 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 5821 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 5881 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 5941 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 6001 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 6061 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 6121 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 6181 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 6241 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 6301 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 6361 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 6421 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 6481 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 6541 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 6601 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 6661 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 6721 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 6781 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 6841 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 6901 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 6961 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 7021 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT //