LOCUS       RC233081                6942 bp    DNA     circular  SYN 
DEFINITION  MLH1 (Myc-DDK tagged) - Homo sapiens mutL homolog 1 (MLH1), transcript variant 5
ACCESSION   NM_001258271
VERSION     NM_001258271.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3099..3190 
                     /label="Myc-DDK"  
     misc_feature    3220..3805 
                     /label="PolyA signal"  
     misc_feature    3277..3296 
                     /label="Restriction Enzyme"  
     misc_feature    4081..4753 
                     /label="ColE1"  
     misc_feature    5183..5977 
                     /label="Kan/Neo"  
     misc_feature    6012..6314 
                     /label="SV40 ori"  
     misc_feature    6400..6877 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3081..3087 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3081 
                     /label="MLH1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGTTCGTG GCAGGGGTTA TTCGGCGGCT GGACGAGACA GTGGTGAACC 
     1081 GCATCGCGGC GGGGGAAGTT ATCCAGCGGC CAGCTAATGC TATCAAAGAG ATGATTGAGA 
     1141 ACTGTTTAGA TGCAAAATCC ACAAGTATTC AAGTGATTGT TAAAGAGGGA GGCCTGAAGT 
     1201 TGATTCAGAT CCAAGACAAT GGCACCGGGA TCAGGAAAGA AGATCTGGAT ATTGTATGTG 
     1261 AAAGGTTCAC TACTAGTAAA CTGCAGTCCT TTGAGGATTT AGCCAGTATT TCTACCTATG 
     1321 GCTTTCGAGG TGAGGCTTTG GCCAGCATAA GCCATGTGGC TCATGTTACT ATTACAACGA 
     1381 AAACAGCTGA TGGAAAGTGT GCATACAGAG CAAGTTACTC AGATGGAAAA CTGAAAGCCC 
     1441 CTCCTAAACC ATGTGCTGGC AATCAAGGGA CCCAGATCAC GGTGGAGGAC CTTTTTTACA 
     1501 ACATAGCCAC GAGGAGAAAA GCTTTAAAAA ATCCAAGTGA AGAATATGGG AAAATTTTGG 
     1561 AAGTTGTTGG CAGGTATTCA GTACACAATG CAGGCATTAG TTTCTCAGTT AAAAAACAAG 
     1621 GAGAGACAGT AGCTGATGTT AGGACACTAC CCAATGCCTC AACCGTGGAC AATATTCGCT 
     1681 CCATCTTTGG AAATGCTGTT AGTCGAGAAC TGATAGAAAT TGGATGTGAG GATAAAACCC 
     1741 TAGCCTTCAA AATGAATGGT TACATATCCA ATGCAAACTA CTCAGTGAAG AAGTGCATCT 
     1801 TCTTACTCTT CATCAACCAT CGTCTGGTAG AATCAACTTC CTTGAGAAAA GCCATAGAAA 
     1861 CAGTGTATGC AGCCTATTTG CCCAAAAACA CACACCCATT CCTGTACCTC AGTTTAGAAA 
     1921 TCAGTCCCCA GAATGTGGAT GTTAATGTGC ACCCCACAAA GCATGAAGTT CACTTCCTGC 
     1981 ACGAGGAGAG CATCCTGGAG CGGGTGCAGC AGCACATCGA GAGCAAGCTC CTGGGCTCCA 
     2041 ATTCCTCCAG GATGTACTTC ACCCAGACTT TGCTACCAGG ACTTGCTGGC CCCTCTGGGG 
     2101 AGATGGTTAA ATCCACAACA AGTCTGACCT CGTCTTCTAC TTCTGGAAGT AGTGATAAGG 
     2161 TCTATGCCCA CCAGATGGTT CGTACAGATT CCCGGGAACA GAAGCTTGAT GCATTTCTGC 
     2221 AGCCTCTGAG CAAACCCCTG TCCAGTCAGC CCCAGGCCAT TGTCACAGAG GATAAGACAG 
     2281 ATATTTCTAG TGGCAGGGCT AGGCAGCAAG ATGAGGAGAT GCTTGAACTC CCAGCCCCTG 
     2341 CTGAAGTGGC TGCCAAAAAT CAGAGCTTGG AGGGGGATAC AACAAAGGGG ACTTCAGAAA 
     2401 TGTCAGAGAA GAGAGGACCT ACTTCCAGCA ACCCCAGAAA GAGACATCGG GAAGATTCTG 
     2461 ATGTGGAAAT GGTGGAAGAT GATTCCCGAA AGGAAATGAC TGCAGCTTGT ACCCCCCGGA 
     2521 GAAGGATCAT TAACCTCACT AGTGTTTTGA GTCTCCAGGA AGAAATTAAT GAGCAGGGAC 
     2581 ATGAGGTTCT CCGGGAGATG TTGCATAACC ACTCCTTCGT GGGCTGTGTG AATCCTCAGT 
     2641 GGGCCTTGGC ACAGCATCAA ACCAAGTTAT ACCTTCTCAA CACCACCAAG CTTAGTGAAG 
     2701 AACTGTTCTA CCAGATACTC ATTTATGATT TTGCCAATTT TGGTGTTCTC AGGTTATCGG 
     2761 AGCCAGCACC GCTCTTTGAC CTTGCCATGC TTGCCTTAGA TAGTCCAGAG AGTGGCTGGA 
     2821 CAGAGGAAGA TGGTCCCAAA GAAGGACTTG CTGAATACAT TGTTGAGTTT CTGAAGAAGA 
     2881 AGGCTGAGAT GCTTGCAGAC TATTTCTCTT TGGAAATTGA TGAGAGTGAA GTGCCTGGCT 
     2941 CCATTCCAAA CTCCTGGAAG TGGACTGTGG AACACATTGT CTATAAAGCC TTGCGCTCAC 
     3001 ACATTCTGCC TCCTAAACAT TTCACAGAAG ATGGAAATAT CCTGCAGCTT GCTAACCTGC 
     3061 CTGATCTATA CAAAGTCTTT GAGAGGTGTA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     3121 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     3181 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     3241 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     3301 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     3361 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     3421 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     3481 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     3541 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     3601 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     3661 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     3721 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     3781 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     3841 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     3901 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     3961 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     4021 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     4081 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     4141 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     4201 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     4261 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     4321 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     4381 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     4441 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     4501 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     4561 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     4621 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     4681 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     4741 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     4801 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     4861 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     4921 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     4981 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     5041 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     5101 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     5161 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     5221 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     5281 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     5341 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     5401 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     5461 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     5521 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     5581 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     5641 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     5701 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     5761 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     5821 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     5881 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     5941 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     6001 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     6061 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     6121 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     6181 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6241 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6301 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     6361 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     6421 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     6481 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     6541 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     6601 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     6661 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     6721 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     6781 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     6841 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     6901 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//