LOCUS       RC231502                7232 bp    DNA     circular  SYN 
DEFINITION  PEG10 (Myc-DDK-tagged)-Human paternally expressed 10 (PEG10), transcript variant 2
ACCESSION   NM_001172437
VERSION     NM_001172437.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3389..3480 
                     /label="Myc-DDK"  
     misc_feature    3510..4095 
                     /label="PolyA signal"  
     misc_feature    3567..3586 
                     /label="Restriction Enzyme"  
     misc_feature    4371..5043 
                     /label="ColE1"  
     misc_feature    5473..6267 
                     /label="Kan/Neo"  
     misc_feature    6302..6604 
                     /label="SV40 ori"  
     misc_feature    6690..7167 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3371..3377 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3371 
                     /label="PEG10"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGAAATAAG CGGGTTTTGA AAACAAAAAA AAGAAGGAGT GGAAGAGGGG 
     1081 GCCAGGATCC AGGCCTCCAT CCCCACAGAA GTGAAGCTAC AGCTGGGAGG TCTCCTCCCA 
     1141 CCCCAACCGT CACCCTGGGT CCCGACTGCC CACCTCCTCC TCCTCCCCCT CCCCCCAACA 
     1201 ACAACAACAA CAACAACTCC AAGCACACCG GCCATAAGAG TGCGTGTGTC CCCAACATGA 
     1261 CCGAACGAAG AAGGGACGAG CTCTCTGAAG AGATCAACAA CTTAAGAGAG AAGGTCATGA 
     1321 AGCAGTCGGA GGAGAACAAC AACCTGCAGA GCCAGGTGCA GAAGCTCACA GAGGAGAACA 
     1381 CCACCCTTCG AGAGCAAGTG GAACCCACCC CTGAGGATGA GGATGATGAC ATCGAGCTCC 
     1441 GCGGTGCTGC AGCAGCTGCT GCCCCACCCC CTCCAATAGA GGAAGAGTGC CCAGAAGACC 
     1501 TCCCAGAGAA GTTCGATGGC AACCCAGACA TGCTGGCTCC TTTCATGGCC CAGTGCCAGA 
     1561 TCTTCATGGA AAAGAGCACC AGGGATTTCT CAGTTGATCG TGTCCGTGTC TGCTTCGTGA 
     1621 CAAGCATGAT GACCGGCCGT GCTGCCCGTT GGGCCTCAGC AAAGCTGGAG CGCTCCCACT 
     1681 ACCTGATGCA CAACTACCCA GCTTTCATGA TGGAAATGAA GCATGTCTTT GAAGACCCTC 
     1741 AGAGGCGAGA GGTTGCCAAA CGCAAGATCA GACGCCTGCG CCAAGGCATG GGGTCTGTCA 
     1801 TCGACTACTC CAATGCTTTC CAGATGATTG CCCAGGACCT GGATTGGAAC GAGCCTGCGC 
     1861 TGATTGACCA GTACCACGAG GGCCTCAGCG ACCACATTCA GGAGGAGCTC TCCCACCTCG 
     1921 AGGTCGCCAA GTCGCTGTCT GCTCTGATTG GGCAGTGCAT TCACATTGAG AGAAGGCTGG 
     1981 CCAGGGCTGC TGCAGCTCGC AAGCCACGCT CGCCACCCCG GGCGCTGGTG TTGCCTCACA 
     2041 TTGCAAGCCA CCACCAGGTA GATCCAACCG AGCCGGTGGG AGGTGCCCGC ATGCGCCTGA 
     2101 CGCAGGAAGA AAAAGAAAGA CGCAGAAAGC TGAACCTGTG CCTCTACTGT GGAACAGGAG 
     2161 GTCACTACGC TGACAATTGT CCTGCCAAGG CCTCAAAGTC TTCGCCGGCG GGAAACTCCC 
     2221 CGGCCCCGCT GTAGAGGGAC CTTCAGCGAC CGGGCCAGAA ATAATAAGGT CCCCACAAGA 
     2281 TGATGCCTCA TCTCCACACT TGCAAGTGAT GCTCCAGATT CATCTTCCGG GCAGACACAC 
     2341 CCTGTTCGTC CGAGCCATGA TCGATTCTGG TGCTTCTGGC AACTTCATTG ATCACGAATA 
     2401 TGTTGCTCAA AATGGAATTC CTCTAAGAAT CAAGGACTGG CCAATACTTG TGGAAGCAAT 
     2461 TGATGGGCGC CCCATAGCAT CGGGCCCAGT TGTCCACGAA ACTCACGACC TGATAGTTGA 
     2521 CCTGGGAGAT CACCGAGAGG TGCTGTCATT TGATGTGACT CAGTCTCCAT TCTTCCCTGT 
     2581 CGTCCTAGGG GTTCGCTGGC TGAGCACACA TGATCCCAAT ATCACATGGA GCACTCGATC 
     2641 TATCGTCTTT GATTCTGAAT ACTGCCGCTA CCACTGCCGG ATGTATTCTC CAATACCACC 
     2701 ATCGCTCCCA CCACCAGCAC CACAACCGCC ACTCTATTAT CCAGTAGATG GATACAGAGT 
     2761 TTACCAACCA GTGAGGTATT ACTATGTCCA GAATGTGTAC ACTCCAGTAG ATGAGCACGT 
     2821 CTACCCAGAT CACCGCCTGG TTGACCCTCA CATAGAAATG ATACCTGGAG CACACAGTAT 
     2881 TCCCAGTGGA CATGTGTATT CACTGTCCGA ACCTGAAATG GCAGCTCTTC GAGATTTTGT 
     2941 GGCAAGAAAT GTAAAAGATG GGCTAATTAC TCCAACGATT GCACCTAATG GAGCCCAAGT 
     3001 TCTCCAGGTG AAGAGGGGGT GGAAACTGCA AGTTTCTTAT GATTGCCGAG CTCCAAACAA 
     3061 TTTTACTATC CAGAATCAGT ATCCTCGCCT ATCTATTCCA AATTTAGAAG ACCAAGCACA 
     3121 CCTGGCAACG TACACTGAAT TCGTACCTCA AATACCTGGA TACCAAACAT ACCCCACATA 
     3181 TGCCGCGTAC CCGACCTACC CAGTAGGATT CGCCTGGTAC CCAGTGGGAC GAGACGGACA 
     3241 AGGAAGATCA CTATATGTAC CTGTGATGAT CACTTGGAAT CCACACTGGT ACCGCCAGCC 
     3301 TCCGGTACCA CAGTACCCGC CGCCACAGCC GCCGCCTCCA CCACCACCAC CGCCGCCGCC 
     3361 TCCATCTTAC AGTACCCTGA CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA 
     3421 GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC 
     3481 CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC 
     3541 CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA 
     3601 AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG 
     3661 GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT 
     3721 GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG 
     3781 TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA 
     3841 GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG 
     3901 TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC 
     3961 AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG 
     4021 AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT 
     4081 GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG 
     4141 GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA 
     4201 GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC 
     4261 CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC 
     4321 AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG 
     4381 TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC 
     4441 CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT 
     4501 CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG 
     4561 CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC 
     4621 TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT 
     4681 GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT 
     4741 ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC 
     4801 AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA 
     4861 AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC 
     4921 GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC 
     4981 CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT 
     5041 ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG 
     5101 GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG 
     5161 GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA 
     5221 CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC 
     5281 TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC 
     5341 CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA 
     5401 TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC 
     5461 ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC 
     5521 GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC 
     5581 CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC 
     5641 CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA 
     5701 AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC 
     5761 TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA 
     5821 CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA 
     5881 ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA 
     5941 CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA 
     6001 GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG 
     6061 TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA 
     6121 GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT 
     6181 CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG 
     6241 CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT 
     6301 CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT 
     6361 AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG 
     6421 GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG 
     6481 ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT 
     6541 GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT 
     6601 GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT 
     6661 CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA 
     6721 TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT 
     6781 TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG 
     6841 TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT 
     6901 TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC 
     6961 TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG 
     7021 GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG 
     7081 AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT 
     7141 CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG 
     7201 AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//