LOCUS       RC231501                7106 bp    DNA     circular  SYN 
DEFINITION  PEG10 (Myc-DDK-tagged)-Human paternally expressed 10 (PEG10), transcript variant 1
ACCESSION   NM_001184961
VERSION     NM_001184961.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3263..3354 
                     /label="Myc-DDK"  
     misc_feature    3384..3969 
                     /label="PolyA signal"  
     misc_feature    3441..3460 
                     /label="Restriction Enzyme"  
     misc_feature    4245..4917 
                     /label="ColE1"  
     misc_feature    5347..6141 
                     /label="Kan/Neo"  
     misc_feature    6176..6478 
                     /label="SV40 ori"  
     misc_feature    6564..7041 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3245..3251 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3245 
                     /label="PEG10"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCCT GGGTCCCGAC TGCCCACCTC CTCCTCCTCC CCCTCCCCCC AACAACAACA 
     1081 ACAACAACAA CTCCAAGCAC ACCGGCCATA AGAGTGCGTG TGTCCCCAAC ATGACCGAAC 
     1141 GAAGAAGGGA CGAGCTCTCT GAAGAGATCA ACAACTTAAG AGAGAAGGTC ATGAAGCAGT 
     1201 CGGAGGAGAA CAACAACCTG CAGAGCCAGG TGCAGAAGCT CACAGAGGAG AACACCACCC 
     1261 TTCGAGAGCA AGTGGAACCC ACCCCTGAGG ATGAGGATGA TGACATCGAG CTCCGCGGTG 
     1321 CTGCAGCAGC TGCTGCCCCA CCCCCTCCAA TAGAGGAAGA GTGCCCAGAA GACCTCCCAG 
     1381 AGAAGTTCGA TGGCAACCCA GACATGCTGG CTCCTTTCAT GGCCCAGTGC CAGATCTTCA 
     1441 TGGAAAAGAG CACCAGGGAT TTCTCAGTTG ATCGTGTCCG TGTCTGCTTC GTGACAAGCA 
     1501 TGATGACCGG CCGTGCTGCC CGTTGGGCCT CAGCAAAGCT GGAGCGCTCC CACTACCTGA 
     1561 TGCACAACTA CCCAGCTTTC ATGATGGAAA TGAAGCATGT CTTTGAAGAC CCTCAGAGGC 
     1621 GAGAGGTTGC CAAACGCAAG ATCAGACGCC TGCGCCAAGG CATGGGGTCT GTCATCGACT 
     1681 ACTCCAATGC TTTCCAGATG ATTGCCCAGG ACCTGGATTG GAACGAGCCT GCGCTGATTG 
     1741 ACCAGTACCA CGAGGGCCTC AGCGACCACA TTCAGGAGGA GCTCTCCCAC CTCGAGGTCG 
     1801 CCAAGTCGCT GTCTGCTCTG ATTGGGCAGT GCATTCACAT TGAGAGAAGG CTGGCCAGGG 
     1861 CTGCTGCAGC TCGCAAGCCA CGCTCGCCAC CCCGGGCGCT GGTGTTGCCT CACATTGCAA 
     1921 GCCACCACCA GGTAGATCCA ACCGAGCCGG TGGGAGGTGC CCGCATGCGC CTGACGCAGG 
     1981 AAGAAAAAGA AAGACGCAGA AAGCTGAACC TGTGCCTCTA CTGTGGAACA GGAGGTCACT 
     2041 ACGCTGACAA TTGTCCTGCC AAGGCCTCAA AGTCTTCGCC GGCGGGAAAC TCCCCGGCCC 
     2101 CGCTGTAGAG GGACCTTCAG CGACCGGGCC AGAAATAATA AGGTCCCCAC AAGATGATGC 
     2161 CTCATCTCCA CACTTGCAAG TGATGCTCCA GATTCATCTT CCGGGCAGAC ACACCCTGTT 
     2221 CGTCCGAGCC ATGATCGATT CTGGTGCTTC TGGCAACTTC ATTGATCACG AATATGTTGC 
     2281 TCAAAATGGA ATTCCTCTAA GAATCAAGGA CTGGCCAATA CTTGTGGAAG CAATTGATGG 
     2341 GCGCCCCATA GCATCGGGCC CAGTTGTCCA CGAAACTCAC GACCTGATAG TTGACCTGGG 
     2401 AGATCACCGA GAGGTGCTGT CATTTGATGT GACTCAGTCT CCATTCTTCC CTGTCGTCCT 
     2461 AGGGGTTCGC TGGCTGAGCA CACATGATCC CAATATCACA TGGAGCACTC GATCTATCGT 
     2521 CTTTGATTCT GAATACTGCC GCTACCACTG CCGGATGTAT TCTCCAATAC CACCATCGCT 
     2581 CCCACCACCA GCACCACAAC CGCCACTCTA TTATCCAGTA GATGGATACA GAGTTTACCA 
     2641 ACCAGTGAGG TATTACTATG TCCAGAATGT GTACACTCCA GTAGATGAGC ACGTCTACCC 
     2701 AGATCACCGC CTGGTTGACC CTCACATAGA AATGATACCT GGAGCACACA GTATTCCCAG 
     2761 TGGACATGTG TATTCACTGT CCGAACCTGA AATGGCAGCT CTTCGAGATT TTGTGGCAAG 
     2821 AAATGTAAAA GATGGGCTAA TTACTCCAAC GATTGCACCT AATGGAGCCC AAGTTCTCCA 
     2881 GGTGAAGAGG GGGTGGAAAC TGCAAGTTTC TTATGATTGC CGAGCTCCAA ACAATTTTAC 
     2941 TATCCAGAAT CAGTATCCTC GCCTATCTAT TCCAAATTTA GAAGACCAAG CACACCTGGC 
     3001 AACGTACACT GAATTCGTAC CTCAAATACC TGGATACCAA ACATACCCCA CATATGCCGC 
     3061 GTACCCGACC TACCCAGTAG GATTCGCCTG GTACCCAGTG GGACGAGACG GACAAGGAAG 
     3121 ATCACTATAT GTACCTGTGA TGATCACTTG GAATCCACAC TGGTACCGCC AGCCTCCGGT 
     3181 ACCACAGTAC CCGCCGCCAC AGCCGCCGCC TCCACCACCA CCACCGCCGC CGCCTCCATC 
     3241 TTACAGTACC CTGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT 
     3301 GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG 
     3361 CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC 
     3421 CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA 
     3481 GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG 
     3541 TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC 
     3601 CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG 
     3661 CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA 
     3721 GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA 
     3781 ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT 
     3841 GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG 
     3901 TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC 
     3961 TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC 
     4021 GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA 
     4081 GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA 
     4141 AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT 
     4201 CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC 
     4261 CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC 
     4321 GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT 
     4381 TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC 
     4441 CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG 
     4501 CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA 
     4561 GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC 
     4621 GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA 
     4681 ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA 
     4741 GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC 
     4801 TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA 
     4861 AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA 
     4921 GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG 
     4981 GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG 
     5041 ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC 
     5101 GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC 
     5161 CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC 
     5221 TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG 
     5281 GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG 
     5341 AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG 
     5401 AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT 
     5461 CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC 
     5521 GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG 
     5581 CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA 
     5641 ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC 
     5701 CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC 
     5761 AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT 
     5821 CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC 
     5881 AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG 
     5941 CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG 
     6001 TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC 
     6061 AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG 
     6121 AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT 
     6181 CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA 
     6241 GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA 
     6301 GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG 
     6361 GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC 
     6421 TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG 
     6481 GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT 
     6541 CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG 
     6601 GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC 
     6661 GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA 
     6721 CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC 
     6781 CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT 
     6841 TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG 
     6901 GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA 
     6961 CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT 
     7021 ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA 
     7081 TTTAACAAAA ATTTAACGCG AATTTT
//