LOCUS       RC231487                9456 bp    DNA     circular  SYN 
DEFINITION  ARHGEF12 (Myc-DDK-tagged)-Human Rho guanine nucleotide exchange factor (GEF) 12 (ARHGEF12), transcript variant 2
ACCESSION   NM_001198665
VERSION     NM_001198665.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5613..5704 
                     /label="Myc-DDK"  
     misc_feature    5734..6319 
                     /label="PolyA signal"  
     misc_feature    5791..5810 
                     /label="Restriction Enzyme"  
     misc_feature    6595..7267 
                     /label="ColE1"  
     misc_feature    7697..8491 
                     /label="Kan/Neo"  
     misc_feature    8526..8828 
                     /label="SV40 ori"  
     misc_feature    8914..9391 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5595..5601 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5595 
                     /label="ARHGEF12"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTGGCACA CAGTCTACTA TCACCGACAG GTTTCCCCTC AAAAAACCTA 
     1081 TAAGGCATGG AAGTATTTTG AACCGAGAGT CACCAACAGA TAAGAAGCAG AAAGTTGAGC 
     1141 GCATTGCATC ACATGATTTT GACCCCACAG GTCTTGTTCA GCGTTGCGTA ATCATCCAGA 
     1201 AAGATGACAA TGGATTTGGG CTGACGGTCA GTGGAGACAA TCCAGTCTTC GTACAGTCTG 
     1261 TCAAAGAAGA TGGAGCAGCC ATGCGGGCTG GAGTACAGAC AGGTGATCGA ATCATCAAGG 
     1321 TGAATGGAAC TCTGGTGACT CATTCAAATC ATCTGGAGGT GGTGAAGCTA ATCAAATCTG 
     1381 GTTCCTATGT AGCTCTCACT GTTCAGGGAC GCCCACCTGG GTCGCCCCAG ATTCCACTTG 
     1441 CCGACTCTGA AGTAGAGCCG TCAGTCATTG GACATATGTC TCCCATCATG ACATCTCCTC 
     1501 ATTCACCTGG AGCATCTGGG AATATGGAGA GAATCACTAG TCCTGTGCTC ATGGGGGAGG 
     1561 AAAACAATGT GGTTCATAAC CAGAAAGTAG AAATTCTGAG AAAAATGTTA CAGAAAGAAC 
     1621 AGGAACGGCT ACAGTTATTG CAGGAAGATT ACAACCGAAC ACCTGCCCAA AGATTGCTAA 
     1681 AAGAGATCCA AGAGGCCAAG AAACACATTC CTCAGCTGCA AGAGCAGTTA TCCAAAGCCA 
     1741 CAGGCTCTGC TCAGGATGGA GCTGTAGTTA CACCCTCCAG ACCTTTAGGG GACACCCTAA 
     1801 CAGTCAGTGA GGCAGAAACA GATCCTGGAG ATGTACTGGG CAGGACTGAC TGTAGCAGTG 
     1861 GAGATGCTTC TCGGCCCAGT AGTGACAATG CAGATAGTCC CAAGAGTGGC CCAAAAGAGA 
     1921 GAATTTATCT AGAGGAAAAC CCAGAGAAAA GTGAAACAAT TCAGGACACT GACACTCAAT 
     1981 CACTTGTCGG AAGTCCCTCA ACCCGTATAG CACCTCATAT TATTGGAGCA GAAGATGATG 
     2041 ATTTTGGTAC TGAACATGAA CAGATCAATG GACAGTGCAG CTGTTTCCAG AGCATTGAAT 
     2101 TACTAAAATC TCGCCCGGCT CATTTGGCTG TTTTCTTACA CCATGTAGTT TCACAATTTG 
     2161 ACCCTGCGAC TTTGCTCTGT TATCTCTATT CAGACCTGTA TAAACATACC AATTCCAAAG 
     2221 AAACTCGTCG CATCTTCCTT GAGTTTCATC AGTTCTTTCT AGATCGATCA GCACACCTGA 
     2281 AAGTTTCTGT TCCTGATGAA ATGTCTGCAG ATCTAGAAAA GAGAAGACCT GAGCTCATTC 
     2341 CTGAGGATCT GCATCGCCAC TATATCCAAA CTATGCAAGA AAGAGTCCAT CCAGAAGTTC 
     2401 AAAGGCACTT AGAAGATTTT CGGCAGAAAC GTAGTATGGG ACTGACCTTG GCTGAAAGCG 
     2461 AGCTGACTAA ACTTGATGCA GAGCGAGACA AGGACCGATT GACTTTGGAG AAGGAGCGGA 
     2521 CATGTGCAGA ACAGATTGTT GCCAAAATTG AAGAAGTATT GATGACTGCT CAGGCTGTAG 
     2581 AGGAAGATAA GAGCTCCACC ATGCAGTATG TTATTCTCAT GTATATGAAG CATTTGGGAG 
     2641 TAAAAGTGAA AGAGCCTCGA AATTTGGAGC ACAAACGGGG TCGGATTGGA TTTCTTCCCA 
     2701 AAATCAAGCA AAGTATGAAG AAAGATAAAG AAGGGGAAGA AAAAGGGAAG CGAAGAGGAT 
     2761 TCCCCAGCAT CCTGGGACCC CCACGGAGAC CAAGCCGTCA TGACAACAGT GCAATTGGCA 
     2821 GAGCCATGGA ACTACAGAAG GCGCGCCACC CTAAGCACTT ATCCACACCC TCATCTGTGA 
     2881 GTCCTGAACC TCAGGACTCT GCCAAGTTGC GCCAGAGTGG GTTAGCAAAT GAAGGAACAG 
     2941 ACGCTGGATA CCTGCCTGCC AATTCCATGT CTTCTGTAGC TTCAGGGGCC TCTTTTTCCC 
     3001 AGGAAGGAGG GAAAGAGAAT GATACAGGAT CAAAGCAAGT TGGAGAAACA TCAGCACCTG 
     3061 GAGACACCTT AGATGGCACA CCTCGTACTC TCAATACTGT CTTTGATTTC CCACCACCTC 
     3121 CATTAGACCA AGTGCAGGAG GAGGAATGTG AAGTAGAAAG GGTGACTGAA CATGGGACAC 
     3181 CAAAGCCCTT TCGAAAGTTT GACAGTGTAG CTTTTGGAGA AAGTCAAAGT GAGGATGAAC 
     3241 AATTTGAAAA TGACTTAGAG ACAGATCCAC CCAACTGGCA GCAGCTTGTT AGTCGAGAAG 
     3301 TGTTACTGGG ACTAAAACCT TGTGAAATCA AAAGACAGGA AGTGATTAAT GAATTGTTCT 
     3361 ACACTGAAAG AGCTCATGTT CGAACACTGA AGGTTCTTGA TCAAGTGTTC TATCAGCGAG 
     3421 TATCCAGAGA AGGAATTCTG TCACCCTCAG AGCTACGGAA AATTTTTTCA AACTTGGAAG 
     3481 ATATTCTTCA ACTTCATATT GGATTGAATG AACAAATGAA GGCTGTTCGA AAGAGAAATG 
     3541 AGACCTCTGT TATCGATCAG ATTGGGGAAG ATTTGCTGAC ATGGTTCAGC GGACCAGGAG 
     3601 AGGAGAAATT GAAACATGCT GCTGCTACCT TTTGCAGTAA CCAACCTTTC GCCCTGGAAA 
     3661 TGATCAAATC TCGTCAGAAA AAGGATTCTC GATTTCAGAC TTTTGTGCAA GATGCTGAAA 
     3721 GTAATCCACT GTGTCGTCGT CTTCAACTGA AGGATATTAT TCCCACTCAA ATGCAAAGGC 
     3781 TTACTAAGTA CCCACTTCTG TTGGATAATA TTGCCAAATA CACAGAATGG CCAACAGAAA 
     3841 GGGAGAAGGT GAAGAAAGCT GCAGATCACT GTCGTCAGAT CTTAAATTAT GTAAATCAGG 
     3901 CTGTCAAGGA GGCAGAAAAC AAGCAGCGCC TAGAAGATTA TCAGCGTCGC CTTGATACCT 
     3961 CCAGCCTGAA GTTGTCAGAG TACCCAAATG TTGAAGAGCT CAGGAATTTG GATTTAACAA 
     4021 AAAGGAAGAT GATTCATGAA GGGCCATTGG TTTGGAAGGT GAATAGAGAT AAAACTATTG 
     4081 ATTTATACAC GTTGCTGCTG GAAGACATTC TTGTATTGTT ACAAAAGCAG GATGATAGAC 
     4141 TGGTTTTAAG GTGTCATAGT AAGATTCTGG CATCTACAGC TGATAGCAAA CACACGTTTA 
     4201 GCCCTGTCAT TAAGTTGAGT ACAGTGTTGG TTCGACAAGT GGCAACAGAT AACAAAGCTT 
     4261 TATTCGTCAT TTCCATGTCA GACAATGGCG CTCAGATTTA TGAACTGGTG GCACAGACAG 
     4321 TTTCTGAAAA GACTGTCTGG CAGGACCTAA TCTGTCGGAT GGCTGCATCA GTGAAGGAGC 
     4381 AATCCACAAA GCCAATTCCA TTACCACAGT CAACACCTGG CGAAGGAGAT AATGATGAAG 
     4441 AAGATCCTTC AAAATTAAAA GAGGAGCAGC ATGGCATTTC AGTCACTGGT TTGCAGAGTC 
     4501 CAGACAGAGA TTTGGGATTA GAATCTACCT TAATATCGTC AAAACCTCAG TCTCATTCAC 
     4561 TGAGTACCTC TGGGAAATCA GAGGTACGTG ATCTGTTTGT GGCTGAGAGA CAGTTTGCAA 
     4621 AGGAACAACA TACAGATGGG ACACTAAAGG AAGTTGGAGA AGATTATCAA ATCGCAATCC 
     4681 CAGATTCACA CCTGCCTGTC TCAGAAGAAC GGTGGGCATT GGATGCACTA AGAAATTTGG 
     4741 GTTTGTTGAA GCAGTTGCTG GTGCAACAGC TAGGTTTGAC TGAGAAGAGC GTTCAGGAAG 
     4801 ACTGGCAACA TTTCCCAAGA TACAGAACAG CCTCTCAGGG GCCGCAGACA GACAGTGTCA 
     4861 TCCAGAACTC TGAAAATATT AAGGCCTATC ATTCTGGTGA AGGACATATG CCCTTTAGAA 
     4921 CTGGAACTGG TGACATTGCA ACTTGTTACA GTCCACGGAC TTCAACTGAA TCTTTTGCTC 
     4981 CACGGGATTC AGTGGGACTG GCACCCCAGG ATAGCCAGGC AAGTAACATT TTAGTAATGG 
     5041 ACCACATGAT TATGACCCCA GAGATGCCTA CCATGGAGCC AGAAGGGGGT CTTGATGACA 
     5101 GTGGAGAGCA CTTTTTTGAT GCCCGTGAAG CACATAGTGA TGAGAATCCA TCAGAAGGTG 
     5161 ATGGAGCAGT TAACAAGGAA GAGAAGGATG TTAATTTACG CATCTCAGGA AACTATTTGA 
     5221 TCCTTGATGG CTATGACCCA GTGCAGGAGA GTTCCACAGA TGAGGAGGTT GCTTCCTCAC 
     5281 TTACCCTGCA GCCCATGACA GGCATCCCTG CTGTGGAATC CACCCACCAG CAGCAACATT 
     5341 CTCCTCAGAA TACTCACTCC GATGGGGCAA TTTCACCATT CACCCCCGAA TTTCTGGTCC 
     5401 AGCAGCGCTG GGGAGCTATG GAGTATTCCT GTTTTGAGAT CCAGAGTCCC TCCTCTTGTG 
     5461 CAGATTCACA GAGCCAGATC ATGGAGTACA TTCATAAGAT AGAGGCTGAC CTTGAACACT 
     5521 TAAAGAAGGT GGAGGAAAGT TACACCATTC TTTGCCAAAG GCTGGCTGGA TCAGCCCTCA 
     5581 CAGACAAGCA CTCAGATAAA AGTACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     5641 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     5701 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     5761 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     5821 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     5881 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5941 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     6001 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     6061 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     6121 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     6181 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     6241 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     6301 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     6361 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     6421 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     6481 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     6541 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     6601 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     6661 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     6721 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     6781 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     6841 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6901 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6961 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     7021 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     7081 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     7141 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     7201 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     7261 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     7321 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     7381 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     7441 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     7501 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     7561 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     7621 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     7681 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     7741 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     7801 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     7861 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7921 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7981 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     8041 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     8101 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     8161 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     8221 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     8281 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     8341 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     8401 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     8461 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     8521 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     8581 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     8641 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     8701 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8761 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8821 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     8881 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8941 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     9001 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     9061 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     9121 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     9181 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     9241 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     9301 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     9361 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     9421 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//