LOCUS       RC231480                8694 bp    DNA     circular  SYN 
DEFINITION  DCTN1 (Myc-DDK-tagged)-Human dynactin 1 (DCTN1), transcript variant 6
ACCESSION   NM_001190837
VERSION     NM_001190837.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4851..4942 
                     /label="Myc-DDK"  
     misc_feature    4972..5557 
                     /label="PolyA signal"  
     misc_feature    5029..5048 
                     /label="Restriction Enzyme"  
     misc_feature    5833..6505 
                     /label="ColE1"  
     misc_feature    6935..7729 
                     /label="Kan/Neo"  
     misc_feature    7764..8066 
                     /label="SV40 ori"  
     misc_feature    8152..8629 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4833..4839 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4833 
                     /label="DCTN1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCACAGAGC AAGAGGCACG TGTACAGCCG GACGCCCAGC GGCAGCAGGA 
     1081 TGAGTGCGGA GGCAAGCGCC CGGCCTCTGC GGGTGGGCTC CCGTGTAGAG GTGATTGGAA 
     1141 AAGGCCACCG AGGCACTGTG GCCTATGTTG GAGCCACACT GTTTGCCACT GGCAAATGGG 
     1201 TAGGCGTGAT TCTGGATGAA GCAAAGGGCA AAAATGATGG AACTGTTCAA GGCAGGAAGT 
     1261 ACTTCACTTG TGATGAAGGG CATGGCATCT TTGTGCGCCA GTCCCAGATC CAGGTATTTG 
     1321 AAGATGGAGC AGATACTACT TCCCCAGAGA CACCTGATTC TTCTGCTTCA AAAGTCCTCA 
     1381 AAAGAGAGGG AACTGATACA ACTGCAAAGA CTAGCAAACT GGCACCGACA GCCCGAAAGA 
     1441 CCACAACTCG GCGACCCAAG CCCACGCGCC CAGCCAGTAC TGGGGTGGCT GGGGCCAGTA 
     1501 GCTCCCTGGG CCCCTCTGGC TCAGCGTCAG CAGGTGAGCT GAGCAGCAGT GAGCCCAGCA 
     1561 CCCCGGCTCA GACTCCGCTG GCAGCACCCA TCATCCCCAC GCCGGTCCTC ACCTCTCCTG 
     1621 GAGCAGTCCC CCCGCTTCCT TCCCCATCCA AGGAGGAGGA GGGACTAAGG GCTCAGGTGC 
     1681 GGGACCTGGA GGAGAAACTA GAGACCCTGA GACTGAAACG GGCAGAAGAC AAAGCAAAGC 
     1741 TAAAAGAGCT GGAGAAACAC AAAATCCAGC TGGAGCAGGT GCAGGAATGG AAGAGCAAAA 
     1801 TGCAGGAGCA GCAGGCCGAC CTGCAGCGGC GCCTCAAGGA GGCGAGAAAG GAAGCCAAGG 
     1861 AGGCGCTGGA GGCAAAGGAA CGCTATATGG AGGAGATGGC TGATACTGCT GATGCCATTG 
     1921 AGATGGCCAC TTTGGACAAG GAGATGGCTG AAGAGCGGGC TGAGTCCCTG CAGCAGGAGG 
     1981 TGGAGGCACT GAAGGAGCGG GTGGACGAGC TCACTACTGA CTTAGAGATC CTCAAGGCTG 
     2041 AGATTGAAGA GAAGGGCTCA GATGGCGCTG CATCCAGTTA TCAGCTCAAG CAGCTTGAGG 
     2101 AGCAGAATGC CCGCCTGAAG GATGCCCTGG TGAGGATGCG GGATCTTTCT TCCTCAGAGA 
     2161 AGCAGGAGCA TGTGAAGCTC CAGAAGCTCA TGGAAAAGAA GAACCAAGAG CTGGAAGTTG 
     2221 TGAGGCAACA GCGGGAGCGT CTGCAGGAGG AGCTAAGCCA GGCAGAGAGC ACCATTGATG 
     2281 AGCTCAAGGA GCAGGTGGAT GCTGCTCTGG GTGCTGAGGA GATGGTGGAG ATGCTGACAG 
     2341 ATCGGAACCT GAATCTGGAA GAGAAAGTGC GCGAGTTGAG GGAGACTGTG GGAGACTTGG 
     2401 AAGCGATGAA TGAGATGAAC GATGAGCTGC AGGAGAATGC ACGTGAGACA GAACTGGAGC 
     2461 TGCGGGAGCA GCTGGACATG GCAGGCGCGC GGGTTCGTGA GGCCCAGAAG CGTGTGGAGG 
     2521 CAGCCCAGGA GACGGTTGCA GACTACCAGC AGACCATCAA GAAGTACCGC CAGCTGACCG 
     2581 CCCATCTACA GGATGTGAAT CGGGAACTGA CAAACCAGCA GGAAGCATCT GTGGAGAGGC 
     2641 AACAGCAGCC ACCTCCAGAG ACCTTTGACT TCAAAATCAA GTTTGCTGAG ACTAAGGCCC 
     2701 ATGCCAAGGC AATTGAGATG GAATTGAGGC AGATGGAGGT GGCCCAGGCC AATCGACACA 
     2761 TGTCCCTGCT GACAGCCTTC ATGCCTGACA GCTTCCTTCG GCCAGGTGGG GACCATGACT 
     2821 GCGTTCTGGT GCTGTTGCTC ATGCCTCGTC TCATTTGCAA GGCAGAGCTG ATCCGGAAGC 
     2881 AGGCCCAGGA GAAGTTTGAA CTAAGTGAGA ACTGTTCAGA GCGGCCTGGG CTGCGAGGAG 
     2941 CTGCTGGGGA GCAACTCAGC TTTGCTGCTG GACTGGTGTA CTCGCTGAGC CTGCTGCAGG 
     3001 CCACGCTACA CCGCTATGAG CATGCCCTCT CTCAGTGCAG TGTGGATGTG TATAAGAAAG 
     3061 TGGGCAGCCT GTACCCTGAG ATGAGTGCCC ATGAGCGCTC CTTGGATTTC CTCATTGAAC 
     3121 TGCTGCACAA GGATCAGCTG GATGAGACTG TCAATGTGGA GCCTCTCACC AAGGCCATCA 
     3181 AGTACTATCA GCATCTGTAC AGCATCCACC TTGCCGAACA GCCTGAGGAC TGTACTATGC 
     3241 AGCTGGCTGA CCACATTAAG TTCACGCAGA GTGCTCTGGA CTGCATGAGT GTGGAGGTAG 
     3301 GACGGCTGCG TGCCTTCTTG CAGGGTGGGC AGGAGGCTAC AGATATTGCC CTCCTGCTCC 
     3361 GGGATCTGGA AACTTCATGC AGTGACATCC GCCAGTTCTG CAAGAAGATC CGAAGGCGAA 
     3421 TGCCAGGGAC AGATGCTCCT GGGATCCCAG CTGCACTGGC CTTTGGACCA CAGGTATCTG 
     3481 ACACGCTCCT AGACTGCAGG AAACACTTGA CGTGGGTCGT GGCTGTGCTG CAGGAGGTGG 
     3541 CAGCTGCTGC TGCCCAGCTC ATTGCCCCAC TGGCAGAGAA TGAGGGGCTA CTTGTGGCTG 
     3601 CTCTGGAGGA ACTGGCTTTC AAAGCAAGCG AGCAGATCTA TGGGACCCCC TCCAGCAGCC 
     3661 CCTATGAGTG TCTGCGCCAG TCATGCAACA TCCTCATCAG TACCATGAAC AAGCTGGCCA 
     3721 CAGCCATGCA GGAGGGGGAG TATGATGCAG AGCGGCCCCC CAGCAAGCCT CCACCGGTTG 
     3781 AACTGCGGGC TGCTGCCCTT CGTGCAGAGA TCACAGATGC TGAAGGCCTG GGTTTGAAGC 
     3841 TCGAAGATCG AGAGACAGTT ATTAAGGAGT TGAAGAAGTC ACTCAAGATT AAGGGAGAGG 
     3901 AGCTAAGTGA GGCCAATGTG CGGCTGAGCC TCCTGGAGAA GAAGTTGGAC AGTGCTGCCA 
     3961 AGGATGCAGA TGAGCGCATC GAGAAAGTCC AGACTCGGCT GGAGGAGACC CAGGCACTGC 
     4021 TGCGAAAGAA GGAGAAAGAG TTTGAGGAGA CAATGGATGC ACTCCAGGCT GACATCGACC 
     4081 AGCTGGAGGC AGAGAAGGCA GAACTAAAGC AGCGTCTGAA CAGCCAGTCC AAACGCACGA 
     4141 TTGAGGGACT CCGGGGCCCT CCTCCTTCAG GCATTGCTAC TCTGGTCTCT GGCATTGCTG 
     4201 GTGAAGAACA GCAGCGAGGA GCCATCCCTG GGCAGGCTCC AGGGTCTGTG CCAGGCCCAG 
     4261 GGCTGGTGAA GGACTCACCA CTGCTGCTTC AGCAGATCTC TGCCATGAGG CTGCACATCT 
     4321 CCCAGCTCCA GCATGAGAAC AGCATCCTCA AGGGAGCCCA GATGAAGGCA TCCTTGGCAT 
     4381 CCCTGCCCCC TCTGCATGTT GCAAAGCTAT CCCATGAGGG CCCTGGCAGT GAGTTACCAG 
     4441 CTGGAGCGCT GTATCGTAAG ACCAGCCAGC TGCTGGAGAC ATTGAATCAA TTGAGCACAC 
     4501 ACACGCACGT AGTAGACATC ACTCGCACCA GCCCTGCTGC CAAGAGCCCG TCGGCCCAAC 
     4561 TTATGGAGCA AGTGGCTCAG CTTAAGTCCC TGAGTGACAC CGTCGAGAAG CTCAAGGATG 
     4621 AGGTCCTCAA GGAGACAGTA TCTCAGCGCC CTGGAGCCAC AGTACCCACT GACTTTGCCA 
     4681 CCTTCCCTTC ATCAGCCTTC CTCAGGGCCA AGGAGGAGCA GCAGGATGAC ACAGTCTACA 
     4741 TGGGCAAAGT GACCTTCTCA TGTGCGGCTG GTTTTGGACA GCGACACCGG CTGGTGCTGA 
     4801 CCCAGGAGCA GCTGCACCAG CTTCACAGTC GCCTCATCTC CACGCGTACG CGGCCGCTCG 
     4861 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4921 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4981 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     5041 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5101 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5161 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5221 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5281 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5341 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5401 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5461 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5521 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5581 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5641 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5701 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5761 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5821 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5881 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5941 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     6001 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6061 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6121 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6181 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6241 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6301 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6361 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6421 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6481 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6541 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6601 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6661 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6721 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6781 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6841 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6901 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6961 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     7021 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7081 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7141 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7201 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7261 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7321 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7381 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7441 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7501 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7561 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7621 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7681 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7741 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7801 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7861 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7921 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7981 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     8041 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8101 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8161 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8221 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8281 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8341 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8401 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8461 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8521 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8581 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8641 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//