LOCUS       RC231476                8589 bp    DNA     circular  SYN 
DEFINITION  DCTN1 (Myc-DDK-tagged)-Human dynactin 1 (DCTN1), transcript variant 5
ACCESSION   NM_001190836
VERSION     NM_001190836.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4746..4837 
                     /label="Myc-DDK"  
     misc_feature    4867..5452 
                     /label="PolyA signal"  
     misc_feature    4924..4943 
                     /label="Restriction Enzyme"  
     misc_feature    5728..6400 
                     /label="ColE1"  
     misc_feature    6830..7624 
                     /label="Kan/Neo"  
     misc_feature    7659..7961 
                     /label="SV40 ori"  
     misc_feature    8047..8524 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4728..4734 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4728 
                     /label="DCTN1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTGCGGAG GCAAGCGCCC GGCCTCTGCG GGTGGGCTCC CGTGTAGAGG 
     1081 TGATTGGAAA AGGCCACCGA GGCACTGTGG CCTATGTTGG AGCCACACTG TTTGCCACTG 
     1141 GCAAATGGGT AGGCGTGATT CTGGATGAAG CAAAGGGCAA AAATGATGGA ACTGTTCAAG 
     1201 GCAGGAAGTA CTTCACTTGT GATGAAGGGC ATGGCATCTT TGTGCGCCAG TCCCAGATCC 
     1261 AGGTATTTGA AGATGGAGCA GATACTACTT CCCCAGAGAC ACCTGATTCT TCTGCTTCAA 
     1321 AAGTCCTCAA AAGAGAGGGA ACTGATACAA CTGCAAAGAC TAGCAAACTG CCCACGCGCC 
     1381 CAGCCAGTAC TGGGGTGGCT GGGGCCAGTA GCTCCCTGGG CCCCTCTGGC TCAGCGTCAG 
     1441 CAGGTGAGCT GAGCAGCAGT GAGCCCAGCA CCCCGGCTCA GACTCCGCTG GCAGCACCCA 
     1501 TCATCCCCAC GCCGGTCCTC ACCTCTCCTG GAGCAGTCCC CCCGCTTCCT TCCCCATCCA 
     1561 AGGAGGAGGA GGGACTAAGG GCTCAGGTGC GGGACCTGGA GGAGAAACTA GAGACCCTGA 
     1621 GACTGAAACG GGCAGAAGAC AAAGCAAAGC TAAAAGAGCT GGAGAAACAC AAAATCCAGC 
     1681 TGGAGCAGGT GCAGGAATGG AAGAGCAAAA TGCAGGAGCA GCAGGCCGAC CTGCAGCGGC 
     1741 GCCTCAAGGA GGCGAGAAAG GAAGCCAAGG AGGCGCTGGA GGCAAAGGAA CGCTATATGG 
     1801 AGGAGATGGC TGATACTGCT GATGCCATTG AGATGGCCAC TTTGGACAAG GAGATGGCTG 
     1861 AAGAGCGGGC TGAGTCCCTG CAGCAGGAGG TGGAGGCACT GAAGGAGCGG GTGGACGAGC 
     1921 TCACTACTGA CTTAGAGATC CTCAAGGCTG AGATTGAAGA GAAGGGCTCA GATGGCGCTG 
     1981 CATCCAGTTA TCAGCTCAAG CAGCTTGAGG AGCAGAATGC CCGCCTGAAG GATGCCCTGG 
     2041 TGAGGATGCG GGATCTTTCT TCCTCAGAGA AGCAGGAGCA TGTGAAGCTC CAGAAGCTCA 
     2101 TGGAAAAGAA GAACCAAGAG CTGGAAGTTG TGAGGCAACA GCGGGAGCGT CTGCAGGAGG 
     2161 AGCTAAGCCA GGCAGAGAGC ACCATTGATG AGCTCAAGGA GCAGGTGGAT GCTGCTCTGG 
     2221 GTGCTGAGGA GATGGTGGAG ATGCTGACAG ATCGGAACCT GAATCTGGAA GAGAAAGTGC 
     2281 GCGAGTTGAG GGAGACTGTG GGAGACTTGG AAGCGATGAA TGAGATGAAC GATGAGCTGC 
     2341 AGGAGAATGC ACGTGAGACA GAACTGGAGC TGCGGGAGCA GCTGGACATG GCAGGCGCGC 
     2401 GGGTTCGTGA GGCCCAGAAG CGTGTGGAGG CAGCCCAGGA GACGGTTGCA GACTACCAGC 
     2461 AGACCATCAA GAAGTACCGC CAGCTGACCG CCCATCTACA GGATGTGAAT CGGGAACTGA 
     2521 CAAACCAGCA GGAAGCATCT GTGGAGAGGC AACAGCAGCC ACCTCCAGAG ACCTTTGACT 
     2581 TCAAAATCAA GTTTGCTGAG ACTAAGGCCC ATGCCAAGGC AATTGAGATG GAATTGAGGC 
     2641 AGATGGAGGT GGCCCAGGCC AATCGACACA TGTCCCTGCT GACAGCCTTC ATGCCTGACA 
     2701 GCTTCCTTCG GCCAGGTGGG GACCATGACT GCGTTCTGGT GCTGTTGCTC ATGCCTCGTC 
     2761 TCATTTGCAA GGCAGAGCTG ATCCGGAAGC AGGCCCAGGA GAAGTTTGAA CTAAGTGAGA 
     2821 ACTGTTCAGA GCGGCCTGGG CTGCGAGGAG CTGCTGGGGA GCAACTCAGC TTTGCTGCTG 
     2881 GACTGGTGTA CTCGCTGAGC CTGCTGCAGG CCACGCTACA CCGCTATGAG CATGCCCTCT 
     2941 CTCAGTGCAG TGTGGATGTG TATAAGAAAG TGGGCAGCCT GTACCCTGAG ATGAGTGCCC 
     3001 ATGAGCGCTC CTTGGATTTC CTCATTGAAC TGCTGCACAA GGATCAGCTG GATGAGACTG 
     3061 TCAATGTGGA GCCTCTCACC AAGGCCATCA AGTACTATCA GCATCTGTAC AGCATCCACC 
     3121 TTGCCGAACA GCCTGAGGAC TGTACTATGC AGCTGGCTGA CCACATTAAG TTCACGCAGA 
     3181 GTGCTCTGGA CTGCATGAGT GTGGAGGTAG GACGGCTGCG TGCCTTCTTG CAGGGTGGGC 
     3241 AGGAGGCTAC AGATATTGCC CTCCTGCTCC GGGATCTGGA AACTTCATGC AGTGACATCC 
     3301 GCCAGTTCTG CAAGAAGATC CGAAGGCGAA TGCCAGGGAC AGATGCTCCT GGGATCCCAG 
     3361 CTGCACTGGC CTTTGGACCA CAGGTATCTG ACACGCTCCT AGACTGCAGG AAACACTTGA 
     3421 CGTGGGTCGT GGCTGTGCTG CAGGAGGTGG CAGCTGCTGC TGCCCAGCTC ATTGCCCCAC 
     3481 TGGCAGAGAA TGAGGGGCTA CTTGTGGCTG CTCTGGAGGA ACTGGCTTTC AAAGCAAGCG 
     3541 AGCAGATCTA TGGGACCCCC TCCAGCAGCC CCTATGAGTG TCTGCGCCAG TCATGCAACA 
     3601 TCCTCATCAG TACCATGAAC AAGCTGGCCA CAGCCATGCA GGAGGGGGAG TATGATGCAG 
     3661 AGCGGCCCCC CAGCAAGCCT CCACCGGTTG AACTGCGGGC TGCTGCCCTT CGTGCAGAGA 
     3721 TCACAGATGC TGAAGGCCTG GGTTTGAAGC TCGAAGATCG AGAGACAGTT ATTAAGGAGT 
     3781 TGAAGAAGTC ACTCAAGATT AAGGGAGAGG AGCTAAGTGA GGCCAATGTG CGGCTGAGCC 
     3841 TCCTGGAGAA GAAGTTGGAC AGTGCTGCCA AGGATGCAGA TGAGCGCATC GAGAAAGTCC 
     3901 AGACTCGGCT GGAGGAGACC CAGGCACTGC TGCGAAAGAA GGAGAAAGAG TTTGAGGAGA 
     3961 CAATGGATGC ACTCCAGGCT GACATCGACC AGCTGGAGGC AGAGAAGGCA GAACTAAAGC 
     4021 AGCGTCTGAA CAGCCAGTCC AAACGCACGA TTGAGGGACT CCGGGGCCCT CCTCCTTCAG 
     4081 GCATTGCTAC TCTGGTCTCT GGCATTGCTG GTGGAGCCAT CCCTGGGCAG GCTCCAGGGT 
     4141 CTGTGCCAGG CCCAGGGCTG GTGAAGGACT CACCACTGCT GCTTCAGCAG ATCTCTGCCA 
     4201 TGAGGCTGCA CATCTCCCAG CTCCAGCATG AGAACAGCAT CCTCAAGGGA GCCCAGATGA 
     4261 AGGCATCCTT GGCATCCCTG CCCCCTCTGC ATGTTGCAAA GCTATCCCAT GAGGGCCCTG 
     4321 GCAGTGAGTT ACCAGCTGGA GCGCTGTATC GTAAGACCAG CCAGCTGCTG GAGACATTGA 
     4381 ATCAATTGAG CACACACACG CACGTAGTAG ACATCACTCG CACCAGCCCT GCTGCCAAGA 
     4441 GCCCGTCGGC CCAACTTATG GAGCAAGTGG CTCAGCTTAA GTCCCTGAGT GACACCGTCG 
     4501 AGAAGCTCAA GGATGAGGTC CTCAAGGAGA CAGTATCTCA GCGCCCTGGA GCCACAGTAC 
     4561 CCACTGACTT TGCCACCTTC CCTTCATCAG CCTTCCTCAG GGCCAAGGAG GAGCAGCAGG 
     4621 ATGACACAGT CTACATGGGC AAAGTGACCT TCTCATGTGC GGCTGGTTTT GGACAGCGAC 
     4681 ACCGGCTGGT GCTGACCCAG GAGCAGCTGC ACCAGCTTCA CAGTCGCCTC ATCTCCACGC 
     4741 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4801 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4861 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4921 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4981 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5041 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5101 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5161 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5221 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5281 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5341 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5401 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5461 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5521 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5581 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5641 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5701 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5761 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5821 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5881 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5941 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6001 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6061 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6121 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6181 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6241 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6301 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6361 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6421 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6481 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6541 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6601 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6661 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6721 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6781 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6841 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6901 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6961 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7021 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7081 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7141 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7201 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7261 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7321 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7381 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7441 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7501 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7561 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7621 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7681 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7741 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7801 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7861 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7921 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7981 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8041 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8101 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8161 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8221 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8281 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8341 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8401 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8461 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8521 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8581 GCGAATTTT
//