LOCUS       RC231473                8514 bp    DNA     circular  SYN 
DEFINITION  NRCAM (Myc-DDK-tagged)-Human neuronal cell adhesion molecule (NRCAM), transcript variant 4
ACCESSION   NM_001193582
VERSION     NM_001193582.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4671..4762 
                     /label="Myc-DDK"  
     misc_feature    4792..5377 
                     /label="PolyA signal"  
     misc_feature    4849..4868 
                     /label="Restriction Enzyme"  
     misc_feature    5653..6325 
                     /label="ColE1"  
     misc_feature    6755..7549 
                     /label="Kan/Neo"  
     misc_feature    7584..7886 
                     /label="SV40 ori"  
     misc_feature    7972..8449 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4653..4659 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4653 
                     /label="NRCAM"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGCTTAAA ATAATGCCGA AAAAGAAGCG CTTATCTGCG GGCAGAGTGC 
     1081 CCCTGATTCT CTTCCTGTGC CAGATGATTA GTGCACTGGA AGTACCTCTT GATCCAAAAC 
     1141 TTCTTGAAGA CTTGGTACAG CCTCCAACCA TCACCCAACA GTCTCCAAAA GATTACATTA 
     1201 TTGACCCTCG GGAGAATATT GTAATCCAGT GTGAAGCCAA AGGGAAACCG CCCCCAAGCT 
     1261 TTTCCTGGAC CCGTAATGGG ACTCATTTTG ACATCGATAA AGACCCTCTG GTCACCATGA 
     1321 AGCCTGGCAC AGGAACGCTC ATAATTAACA TCATGAGCGA AGGGAAAGCT GAGACCTATG 
     1381 AAGGAGTCTA TCAGTGTACA GCAAGGAACG AACGCGGAGC TGCAGTTTCT AATAACATTG 
     1441 TTGTCCGCCC ATCCAGATCA CCATTGTGGA CCAAAGAAAA ACTTGAACCA ATCACACTTC 
     1501 AAAGTGGTCA GTCTTTAGTA CTTCCCTGCA GACCCCCAAT TGGATTACCA CCACCTATAA 
     1561 TATTTTGGAT GGATAATTCC TTTCAAAGAC TTCCACAAAG TGAGAGAGTT TCTCAAGGTT 
     1621 TGAATGGGGA CCTTTATTTT TCCAATGTCC TCCCAGAGGA CACCCGCGAA GACTATATCT 
     1681 GTTATGCTAG ATTTAATCAT ACTCAAACCA TACAGCAGAA GCAACCTATT TCTGTGAAGG 
     1741 TGATTTCAGT GGATGAATTG AATGACACTA TAGCTGCTAA TTTGAGTGAC ACTGAGTTTT 
     1801 ATGGTGCTAA ATCAAGTAGA GAGAGGCCAC CAACATTTTT AACTCCAGAA GGCAATGCAA 
     1861 GTAACAAAGA GGAATTAAGA GGAAATGTGC TTTCACTGGA GTGCATTGCA GAAGGACTGC 
     1921 CTACCCCAAT TATTTACTGG GCAAAGGAAG ATGGAATGCT ACCCAAAAAC AGGACAGTTT 
     1981 ATAAGAACTT TGAGAAAACC TTGCAGATCA TTCATGTTTC AGAAGCAGAC TCTGGAAATT 
     2041 ACCAATGTAT AGCAAAAAAC GCATTAGGAG CCATCCACCA TACCATTTCT GTTAGAGTTA 
     2101 AAGCGGCTCC ATACTGGATC ACAGCCCCTC AAAATCTTGT GCTGTCCCCA GGAGAGGATG 
     2161 GGACCTTGAT CTGCAGAGCT AATGGCAACC CCAAACCCAG AATTAGCTGG TTAACAAATG 
     2221 GAGTCCCAAT AGAAATTGCC CCTGATGACC CCAGCAGAAA AATAGATGGC GATACCATTA 
     2281 TTTTTTCAAA TGTTCAAGAA AGATCAAGTG CAGTCTATCA GTGCAATGCC TCTAATGAAT 
     2341 ATGGATATTT ACTGGCAAAC GCATTTGTAA ATGTGCTGGC TGAGCCACCA CGAATCCTCA 
     2401 CACCTGCAAA CACACTCTAC CAGGTCATTG CAAACAGGCC TGCTTTACTA GACTGTGCCT 
     2461 TCTTTGGGTC TCCTCTCCCA ACCATCGAGT GGTTTAAAGG AGCTAAAGGA AGTGCTCTTC 
     2521 ATGAAGATAT TTATGTTTTA CATGAAAATG GAACTTTGGA AATTCCTGTG GCCCAAAAGG 
     2581 ACAGTACAGG AACTTATACG TGTGTTGCAA GGAATAAATT AGGGATGGCG AAGAATGAAG 
     2641 TTCACTTAGA AATCAAAGAT CCTACATGGA TCGTTAAACA GCCCGAATAT GCAGTTGTGC 
     2701 AAAGAGGGAG CATGGTGTCC TTTGAATGCA AAGTGAAACA TGATCACACC TTATCCCTCA 
     2761 CTGTCCTGTG GCTGAAGGAC AACAGGGAAC TGCCCAGTGA TGAAAGGTTC ACTGTTGACA 
     2821 AGGATCATCT AGTGGTAGCT GATGTCAGTG ACGATGACAG CGGGACCTAC ACGTGTGTGG 
     2881 CCAACACCAC TCTGGACAGC GTCTCCGCCA GCGCTGTGCT TAGCGTTGTT GCTCCTACTC 
     2941 CAACTCCAGC TCCCGTTTAC GATGTCCCAA ATCCTCCCTT TGACTTAGAA CTGACAGATC 
     3001 AACTTGACAA AAGTGTTCAG CTGTCATGGA CCCCAGGCGA TGACAACAAT AGCCCCATTA 
     3061 CAAAATTCAT CATCGAATAT GAAGATGCAA TGCACAAGCC AGGGCTGTGG CACCACCAAA 
     3121 CTGAAGTTTC TGGAACACAG ACCACAGCCC AGCTGAAGCT GTCTCCTTAC GTGAACTACT 
     3181 CCTTCCGCGT GATGGCAGTG AACAGCATTG GGAAGAGCTT GCCCAGCGAG GCGTCTGAGC 
     3241 AGTATTTGAC GAAAGCCTCA GAACCAGATA AAAACCCCAC AGCTGTGGAA GGACTGGGAT 
     3301 CAGAGCCTGA TAATTTGGTG ATTACGTGGA AGCCCTTGAA TGGTTTCGAA TCTAATGGGC 
     3361 CAGGCCTTCA GTACAAAGTT AGCTGGCGCC AGAAAGATGG TGATGATGAA TGGACATCTG 
     3421 TGGTTGTGGC AAATGTATCC AAATATATTG TCTCAGGCAC GCCAACCTTT GTTCCATACC 
     3481 TGATCAAAGT TCAGGCCCTG AATGACATGG GGTTTGCCCC CGAGCCAGCT GTAGTCATGG 
     3541 GACATTCTGG AGAAGACCTC CCAATGGTGG CTCCTGGGAA CGTGCGTGTG AATGTGGTGA 
     3601 ACAGTACCTT AGCCGAGGTG CACTGGGACC CAGTACCTCT GAAAAGCATC CGAGGACACC 
     3661 TACAAGGCTA TCGGATTTAC TATTGGAAGA CCCAGAGTTC ATCTAAAAGA AACAGACGTC 
     3721 ACATTGAGAA AAAGATCCTC ACCTTCCAAG GCAGCAAGAC TCATGGCATG TTGCCGGGGC 
     3781 TAGAGCCCTT TAGCCACTAC ACACTGAATG TCCGAGTGGT CAATGGGAAA GGGGAGGGCC 
     3841 CAGCCAGCCC TGACAGAGTC TTTAATACTC CAGAAGGAGT CCCCAGTGCT CCCTCGTCTT 
     3901 TGAAGATTGT GAATCCAACA CTGGACTCTC TCACTTTGGA ATGGGATCCA CCGAGCCACC 
     3961 CGAATGGCAT TTTGACAGAG TACACCTTAA AGTATCAGCC AATTAACAGC ACACATGAAT 
     4021 TAGGCCCTCT GGTAGATTTG AAAATTCCTG CCAACAAGAC ACGGTGGACT TTAAAAAATT 
     4081 TAAATTTCAG CACTCGATAT AAGTTTTATT TCTATGCACA AACATCAGCA GGATCAGGAA 
     4141 GTCAAATTAC AGAGGAAGCA GTAACAACTG TGGATGAAGC TGGTATTCTT CCACCTGATG 
     4201 TAGGTGCAGG CAAAGCGATG GCAAGCCGGC AGGTGGATAT TGCAACTCAG GGCTGGTTCA 
     4261 TTGGTCTGAT GTGTGCTGTT GCTCTCCTTA TCTTAATTTT GCTGATTGTT TGCTTCATCA 
     4321 GAAGAAACAA GGGTGGTAAA TATCCAGTTA AAGAAAAGGA AGATGCCCAT GCTGACCCTG 
     4381 AAATCCAGCC TATGAAGGAA GATGATGGGA CATTTGGAGA ATACAGTGAT GCAGAAGACC 
     4441 ACAAGCCTTT GAAAAAAGGA AGTCGAACTC CTTCAGACAG GACTGTGAAA AAAGAAGATA 
     4501 GTGACGACAG CCTAGTTGAC TATGGAGAAG GGGTTAATGG CCAGTTCAAT GAGGATGGCT 
     4561 CCTTTATTGG ACAATACAGT GGTAAGAAAG AGAAAGAGCC GGCTGAAGGA AACGAAAGCT 
     4621 CAGAGGCACC TTCTCCTGTC AACGCCATGA ATTCCTTTGT TACGCGTACG CGGCCGCTCG 
     4681 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4741 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4801 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4861 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4921 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4981 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5041 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5101 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5161 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5221 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5281 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5341 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5401 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5461 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5521 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5581 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5641 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5701 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5761 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5821 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5881 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5941 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6001 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6061 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6121 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6181 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6241 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6301 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6361 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6421 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6481 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6541 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6601 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6661 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6721 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6781 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6841 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6901 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6961 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7021 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7081 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7141 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7201 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7261 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7321 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7381 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7441 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7501 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7561 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7621 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7681 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7741 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7801 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7861 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7921 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7981 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8041 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8101 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8161 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8221 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8281 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8341 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8401 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8461 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//