LOCUS       RC231471                8355 bp    DNA     circular  SYN 
DEFINITION  STON1 (Myc-DDK-tagged)-Human STON1-GTF2A1L readthrough (STON1-GTF2A1L), transcript variant 2
ACCESSION   NM_001198593
VERSION     NM_001198593.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4512..4603 
                     /label="Myc-DDK"  
     misc_feature    4633..5218 
                     /label="PolyA signal"  
     misc_feature    4690..4709 
                     /label="Restriction Enzyme"  
     misc_feature    5494..6166 
                     /label="ColE1"  
     misc_feature    6596..7390 
                     /label="Kan/Neo"  
     misc_feature    7425..7727 
                     /label="SV40 ori"  
     misc_feature    7813..8290 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4494..4500 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4494 
                     /label="STON1-GTF2A1L"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGCTCCACA AATCCAGGCA AATGGGTCAC CTTTGATGAT GATCCTGCTG 
     1081 TTCAATCTTC TCAAAAGTCA AAGAATTTTC CTCTGGAGAA TCAAGGTGTC TGTAGACCAA 
     1141 ATGGACTGAA GCTGAACCTT CCTGGCCTCA GGGAATTTCC CAGTGGATCT TCCTCCACCA 
     1201 GCAGCACTCC TCTCTCCTCC CCCATTGTAG ATTTTTATTT CAGTCCAGGA CCTCCAAGTA 
     1261 ACTCTCCTCT TTCTACACCT ACCAAAGACT TCCCAGGTTT TCCTGGCATC CCCAAAGCAG 
     1321 GGACTCATGT GCTTTATCCT ATTCCAGAAT CATCTTCAGA CAGCCCACTC GCAATATCAG 
     1381 GAGGAGAATC TTCCTTACTG CCTACCAGAC CAACATGTTT ATCCCATGCC TTGTTACCCA 
     1441 GTGACCACTC ATGTACACAT CCAACTCCCA AAGTAGGTCT TCCAGATGAA GTTAATCCTC 
     1501 AACAGGCTGA AAGCCTAGGA TTCCAAAGTG ATGATCTCCC CCAGTTTCAG TATTTTCGAG 
     1561 AGGACTGTGC TTTTTCAAGT CCATTTTGGA AAGATGAAGG CAGTGATTCC CATTTCACCC 
     1621 TTGACCCACC AGGAAGCAAA AAGATGTTCT CATCAAGAAA CAAGGAGATG CCTATTGACC 
     1681 AAAAAAGCCT AAATAAGTGT TCACTCAACT ATATCTGTGA GAAGCTTGAA CATCTCCAGT 
     1741 CAGCTGAGAA CCAAGACTCA CTTAGAAGTT TGTCTATGCA CTGTCTATGT GCTGAAGAAA 
     1801 ATGCCTCTTC CTTTGTCCCC CACACACTCT TCAGGAGTCA GCCAAAATCC GGATGGTCTT 
     1861 TCATGCTGAG AATTCCTGAG AAGAAGAATA TGATGTCTTC CCGGCAATGG GGACCAATTT 
     1921 TTCTGAAAGT TTTGCCTGGA GGAATTTTGC AGATGTATTA TGAACAGGGA TTAGAAAAAC 
     1981 CATTTAAAGA GATACAGCTT GATCCATATT GTAGGCTTTC TGAACCCAAG GTTGAGAACT 
     2041 TCAGTGTAGC AGGAAAAATC CACACTGTGA AGATTGAACA TGTGTCTTAC ACAGAAAAAA 
     2101 GGAAATACCA TTCTAAGACA GAAGTAGTTC ATGAACCTGA CATAGAGCAG ATGCTGAAGT 
     2161 TGGGGTCCAC ATCGTACCAT GACTTCCTTG ACTTTCTGAC TACTGTGGAG GAGGAGCTGA 
     2221 TGAAGTTGCC AGCTGTTTCA AAACCAAAAA AGAACTACGA GGAGCAAGAA ATTTCCTTGG 
     2281 AAATTGTGGA CAACTTTTGG GGTAAAGTCA CAAAAGAAGG AAAATTTGTT GAAAGTGCTG 
     2341 TGATAACTCA AATTTATTGC CTCTGCTTTG TGAATGGGAA CCTGGAATGC TTTTTAACCT 
     2401 TGAATGACCT TGAGTTGCCG AAGCGAGATG AATCCTATTA TGAGAAGGAC TCAGAAAAAA 
     2461 AGGGGATTGA TATTCTTGAC TACCATTTTC ATAAGTGTGT GAATGTACAA GAATTTGAGC 
     2521 AATCAAGAAT CATTAAGTTT GTACCTCTGG ATGCCTGCCG GTTTGAGCTG ATGCGTTTCA 
     2581 AGACTTTGTA TAATGGGGAT AATCTTCCCT TTTCCTTGAA GTCTGTAGTG GTTGTCCAGG 
     2641 GAGCATACGT GGAACTTCAG GCTTTTGTCA ACATGGCCTC ATTGGCGCAG AGGTCATCCT 
     2701 ATGCTGGTTC CTTAAGGTCC TGTGACAATA TAAGGATACA CTTTCCTGTC CCATCGCAGT 
     2761 GGATCAAGGC CCTTTGGACC ATGAACCTCC AGAGGCAGAA GTCTCTGAAA GCTAAAATGA 
     2821 ACCGCCGAGC ATGTCTGGGG AGTTTACAGG AACTTGAATC TGAACCTGTC ATTCAAGTCA 
     2881 CTGTGGGGTC AGCAAAATAT GAGAGTGCCT ACCAGGCAGT GGTATGGAAG ATAGATCGGC 
     2941 TTCCAGACAA AAATTCAAGT CTAGATCATC CCCATTGTCT GTCATACAAA TTAGAGCTTG 
     3001 GATCAGACCA AGAAATTCCC TCTGATTGGT ATCCATTTGC TACTGTTCAG TTTTCCGTGC 
     3061 CTGACACCTG TGCCTCAAGG ACAGAGGTCA GGTCTCTGGG AGTGGAGAGT GATGTCCAGC 
     3121 CACAGAAACA TGTTCAGCAG CGAGCTTGCT ACAACATCCA GCCTAAACTC TACAGATCTG 
     3181 TAATTGAAGA TGTAATTGAA GGAGTTCGGA ATCTATTTGC TGAAGAAGGT ATAGAGGAAC 
     3241 AAGTTTTAAA AGACTTGAAG CAGCTCTGGG AAACCAAGGT TTTGCAGTCT AAAGCAACAG 
     3301 AAGACTTCTT CAGAAATAGC ATCCAATCAC CTCTGTTTAC TCTTCAGTTG CCGCACAGCT 
     3361 TGCACCAAAC ATTGCAATCG TCAACAGCAT CATTAGTTAT TCCTGCTGGT AGAACTCTTC 
     3421 CAAGTTTTAC CACAGCAGAA CTGGGCACTT CAAACTCCAG TGCAAACTTT ACTTTTCCTG 
     3481 GTTATCCCAT TCATGTACCA GCAGGTGTGA CACTACAGAC TGTATCTGGT CACCTTTATA 
     3541 AAGTCAATGT ACCAATTATG GTGACAGAGA CTTCTGGAAG AGCAGGTATT CTTCAGCATC 
     3601 CAATTCAGCA AGTATTTCAA CAGCTTGGCC AGCCTTCAGT AATACAAACT AGTGTTCCAC 
     3661 AATTGAATCC ATGGTCTCTT CAAGCAACTA CTGAAAAATC ACAGAGAATT GAAACCGTGC 
     3721 TACAGCAACC CGCAATTCTA CCTTCTGGGC CAGTAGATAG GAAACACTTA GAAAATGCCA 
     3781 CCAGTGATAT ACTTGTATCT CCTGGAAATG AGCATAAAAT CGTGCCTGAA GCTTTGTTGT 
     3841 GTCATCAGGA AAGTTCTCAC TATATCAGTC TTCCAGGTGT TGTATTTTCT CCACAGGTCT 
     3901 CTCAAACAAA TTCTAATGTG GAGTCAGTGC TCAGTGGTTC AGCTAGCATG GCTCAAAATC 
     3961 TGCATGATGA GTCCCTCTCC ACAAGCCCTC ATGGGGCTCT CCACCAGCAC GTGACTGATA 
     4021 TTCAGCTTCA TATTCTTAAA AATAGGATGT ATGGATGTGA TTCTGTAAAG CAACCAAGAA 
     4081 ATATAGAGGA ACCCAGCAAC ATACCTGTAT CAGAGAAGGA TTCTAATTCT CAGGTGGATT 
     4141 TAAGCATTCG GGTTACTGAT GATGATATTG GTGAAATAAT TCAAGTAGAT GGAAGCGGTG 
     4201 ATACATCTTC CAATGAAGAA ATAGGAAGTA CAAGAGATGC AGATGAGAAT GAATTTCTAG 
     4261 GGAATATTGA CGGGGGAGAT CTGAAGGTAC CTGAAGAAGA AGCTGACAGT ATTTCAAATG 
     4321 AGGATTCAGC CACAAACAGT AGTGATAATG AAGACCCTCA AGTAAACATT GTAGAAGAGG 
     4381 ACCCTTTAAA TTCTGGAGAT GATGTTAGTG AACAGGATGT GCCAGACCTG TTTGACACGG 
     4441 ATAATGTTAT TGTCTGTCAG TATGATAAGC AGGGAGATAC CAGGGTTCGT CATGGGCAGC 
     4501 AAACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4561 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4621 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4681 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4741 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4801 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4861 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4921 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4981 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5041 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5101 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5161 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5221 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5281 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5341 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5401 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5461 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5521 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5581 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5641 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5701 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5761 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5821 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5881 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5941 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6001 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6061 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6121 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6181 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6241 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6301 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6361 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6421 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6481 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6541 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6601 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6661 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6721 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6781 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6841 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6901 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6961 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7021 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7081 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7141 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7201 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7261 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7321 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7381 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7441 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7501 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7561 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7621 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7681 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7741 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7801 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7861 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7921 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7981 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8041 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8101 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8161 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8221 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8281 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8341 TTTAACGCGA ATTTT
//