LOCUS       RC231470                8310 bp    DNA     circular  SYN 
DEFINITION  MCM9 (Myc-DDK-tagged)-Human minichromosome maintenance complex component 9 (MCM9), transcript variant 1
ACCESSION   NM_017696
VERSION     NM_017696.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4467..4558 
                     /label="Myc-DDK"  
     misc_feature    4588..5173 
                     /label="PolyA signal"  
     misc_feature    4645..4664 
                     /label="Restriction Enzyme"  
     misc_feature    5449..6121 
                     /label="ColE1"  
     misc_feature    6551..7345 
                     /label="Kan/Neo"  
     misc_feature    7380..7682 
                     /label="SV40 ori"  
     misc_feature    7768..8245 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4449..4455 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4449 
                     /label="MCM9"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAATAGCGAT CAAGTTACAC TGGTTGGTCA AGTGTTTGAG TCATATGTTT 
     1081 CGGAATACCA TAAGAATGAT ATTCTTCTAA TCTTGAAGGA AAGGGATGAA GATGCTCATT 
     1141 ACCCAGTTGT GGTTAATGCC ATGACTCTGT TTGAGACCAA CATGGAAATC GGGGAATATT 
     1201 TCAACATGTT CCCCAGTGAA GTGCTTACAA TTTTTGATAG TGCACTGCGA AGGTCAGCCT 
     1261 TGACAATTCT CCAGTCCCTT TCTCAGCCTG AGGCTGTTTC CATGAAACAG AATCTTCATG 
     1321 CCAGGATATC AGGTTTGCCT GTCTGTCCTG AGCTGGTGAG GGAACACATA CCTAAAACCA 
     1381 AGGATGTGGG ACACTTTTTA TCTGTCACTG GGACAGTGAT TCGAACAAGT CTGGTGAAGG 
     1441 TTCTGGAGTT TGAGCGGGAT TACATGTGTA ACAAATGCAA GCATGTGTTT GTGATCAAGG 
     1501 CTGACTTTGA GCAGTATTAC ACCTTTTGCC GGCCATCCTC GTGTCCCAGC TTGGAGAGCT 
     1561 GTGATTCCTC TAAATTCACT TGCCTCTCAG GCTTGTCTTC GTCTCCAACC AGGTGTAGAG 
     1621 ATTACCAGGA AATCAAAATT CAGGAACAGG TTCAAAGGCT ATCTGTTGGA AGTATTCCAC 
     1681 GATCTATGAA GGTTATTCTG GAAGATGACT TAGTGGATAG TTGCAAATCT GGTGATGACC 
     1741 TCACTATTTA CGGGATTGTA ATGCAACGGT GGAAGCCCTT TCAGCAAGAT GTGCGCTGTG 
     1801 AAGTGGAGAT AGTCCTGAAA GCAAATTACA TCCAAGTAAA TAATGAGCAG TCCTCAGGGA 
     1861 TCATCATGGA TGAGGAGGTC CAAAAGGAAT TCGAAGATTT TTGGGAATAC TATAAGAGCG 
     1921 ATCCCTTTGC AGGAAGGAAT GTAATATTGG CTAGCTTGTG CCCTCAAGTG TTTGGAATGT 
     1981 ATCTAGTAAA GCTTGCTGTG GCCATGGTGC TGGCTGGTGG GATTCAAAGG ACTGATGCTA 
     2041 CAGGAACACG GGTCAGAGGA GAATCTCATC TTTTATTGGT TGGGGATCCT GGCACAGGGA 
     2101 AATCTCAGTT CCTCAAATAT GCAGCAAAGA TTACACCAAG ATCTGTGCTG ACCACAGGAA 
     2161 TTGGATCTAC TAGTGCAGGT CTGACGGTAA CTGCTGTAAA AGACTCAGGA GAATGGAATT 
     2221 TGGAGGCTGG GGCATTAGTT CTTGCAGATG CGGGCCTTTG CTGTATTGAT GAGTTCAATA 
     2281 GCCTCAAAGA GCATGATAGG ACCAGTATCC ATGAAGCAAT GGAGCAACAA ACCATAAGTG 
     2341 TTGCTAAGGC TGGCCTCGTG TGCAAGCTGA ACACAAGGAC CACCATCCTG GCAGCAACGA 
     2401 ACCCCAAAGG CCAGTACGAC CCCCAGGAGT CCGTGTCTGT GAACATTGCC CTCGGCAGCC 
     2461 CACTCTTAAG TCGATTTGAC CTGATCCTGG TTTTGCTTGA TACCAAGAAT GAAGACTGGG 
     2521 ATCGTATCAT TTCCTCCTTT ATCTTAGAAA ATAAAGGTTA CCCAAGCAAA TCAGAGAAGC 
     2581 TCTGGAGCAT GGAAAAGATG AAAACCTATT TCTGCCTCAT AAGGAATCTG CAGCCCACAC 
     2641 TGTCTGATGT GGGCAATCAG GTTCTTCTCC GGTACTACCA GATGCAAAGG CAGAGTGATT 
     2701 GCCGGAACGC TGCCCGGACC ACCATTCGGC TGTTGGAAAG CTTGATACGA TTAGCAGAAG 
     2761 CTCATGCTCG CCTGATGTTT CGTGATACTG TAACTCTGGA AGACGCTATT ACGGTGGTGT 
     2821 CAGTCATGGA GTCCTCAATG CAGGGAGGTG CACTGCTAGG AGGTGTGAAT GCCCTCCACA 
     2881 CTTCCTTTCC TGAAAACCCT GGAGAGCAGT ACCAGAGACA GTGTGAACTT ATTCTGGAAA 
     2941 AGCTAGAGCT GCAGAGCCTC TTGAGTGAAG AGCTTAGAAG ACTTGAAAGG TTACAGAATC 
     3001 AGAGTGTGCA CCAATCCCAA CCACGGGTAT TGGAGGTAGA GACTACTCCA GGATCCTTGA 
     3061 GAAATGGTCC AGGGGAAGAA TCAAACTTCA GAACTTCATC ACAGCAGGAA ATCAACTATA 
     3121 GCACACATAT CTTCTCTCCT GGAGGCAGCC CCGAGGGAAG CCCAGTTCTA GATCCCCCAC 
     3181 CGCATCTGGA GCCTAATAGA TCAACAAGTA GGAAACATTC AGCTCAGCAC AAAAATAACA 
     3241 GAGATGACAG TTTAGATTGG TTTGATTTCA TGGCAACTCA TCAGAGTGAA CCTAAAAACA 
     3301 CTGTTGTTGT GTCTCCTCAT CCCAAAACAT CTGGAGAAAA TATGGCTTCG AAGATCTCTA 
     3361 ACAGCACATC TCAGGGTAAG GAGAAGAGTG AGCCAGGCCA AAGGAGCAAA GTGGACATTG 
     3421 GGTTGCTTCC ATCACCAGGA GAGACAGGTG TTCCATGGAG GGCAGACAAT GTGGAAAGTA 
     3481 ACAAGAAAAA AAGGCTAGCA CTAGATTCTG AAGCAGCAGT CTCTGCTGAT AAACCAGACT 
     3541 CAGTACTGAC TCATCATGTC CCCAGGAACC TGCAGAAGCT GTGCAAAGAG AGGGCCCAGA 
     3601 AGTTGTGCAG AAATAGCACC AGGGTGCCTG CACAGTGCAC AGTCCCTTCC CATCCTCAGT 
     3661 CCACTCCTGT ACATAGCCCA GACAGAATGC TGGACTCACC CAAAAGAAAG AGACCGAAAT 
     3721 CCCTTGCGCA AGTGGAAGAG CCTGCAATTG AAAATGTTAA GCCTCCAGGT TCCCCTGTGG 
     3781 CCAAACTGGC AAAATTTACT TTCAAGCAGA AGTCAAAACT GATCCACTCC TTTGAAGATC 
     3841 ACAGCCATGT GTCACCTGGT GCAACTAAAA TAGCAGTTCA TAGTCCTAAA ATTTCCCAGC 
     3901 GTAGAACAAG AAGAGACGCA GCCTTGCCGG TGAAGCGTCC AGGAAAGTTA ACATCTACCC 
     3961 CAGGAAACCA GATCTCCAGT CAGCCACAGG GTGAGACAAA GGAGGTGTCG CAGCAGCCAC 
     4021 CAGAGAAACA CGGACCAAGA GAGAAGGTGA TGTGTGCCCC TGAGAAGAGG ATTATTCAGC 
     4081 CTGAATTAGA GCTTGGGAAC GAGACTGGGT GTGCTCATCT TACTTGTGAG GGAGACAAAA 
     4141 AGGAAGAGGT TTCAGGCAGT AATAAAAGCG GCAAGGTTCA TGCCTGCACA TTAGCCAGAT 
     4201 TGGCAAACTT CTGCTTTACT CCCCCATCGG AATCCAAATC AAAATCCCCT CCTCCTGAAA 
     4261 GGAAGAACCG AGGTGAGAGA GGCCCAAGCT CCCCTCCTAC AACCACAGCT CCAATGCGTG 
     4321 TCAGTAAAAG GAAATCTTTT CAGCTCCGTG GGTCCACCGA GAAACTGATT GTTTCCAAAG 
     4381 AATCCCTCTT CACTTTACCA GAACTAGGTG ATGAAGCATT TGATTGTGAC TGGGATGAAG 
     4441 AGATGAGAAA AAAGTCAACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4501 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4561 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4621 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4681 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4741 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4801 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4861 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4921 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4981 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5041 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5101 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5161 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5221 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5281 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5341 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5401 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5461 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5521 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5581 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5641 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5701 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5761 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5821 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5881 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     5941 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6001 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6061 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6121 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6181 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6241 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6301 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6361 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6421 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6481 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6541 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6601 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6661 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6721 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6781 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6841 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6901 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     6961 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7021 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7081 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7141 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7201 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7261 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7321 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7381 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7441 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7501 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7561 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7621 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7681 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7741 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7801 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7861 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7921 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     7981 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8041 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8101 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8161 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8221 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8281 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//