LOCUS       RC231439                7599 bp    DNA     circular  SYN 
DEFINITION  LEPR (Myc-DDK-tagged)-Human leptin receptor (LEPR), transcript variant 5
ACCESSION   NM_001198688
VERSION     NM_001198688.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3756..3847 
                     /label="Myc-DDK"  
     misc_feature    3877..4462 
                     /label="PolyA signal"  
     misc_feature    3934..3953 
                     /label="Restriction Enzyme"  
     misc_feature    4738..5410 
                     /label="ColE1"  
     misc_feature    5840..6634 
                     /label="Kan/Neo"  
     misc_feature    6669..6971 
                     /label="SV40 ori"  
     misc_feature    7057..7534 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3738..3744 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3738 
                     /label="LEPR"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATTTGTCAA AAATTCTGTG TGGTTTTGTT ACATTGGGAA TTTATTTATG 
     1081 TGATAACTGC GTTTAACTTG TCATATCCAA TTACTCCTTG GAGATTTAAG TTGTCTTGCA 
     1141 TGCCACCAAA TTCAACCTAT GACTACTTCC TTTTGCCTGC TGGACTCTCA AAGAATACTT 
     1201 CAAATTCGAA TGGACATTAT GAGACAGCTG TTGAACCTAA GTTTAATTCA AGTGGTACTC 
     1261 ACTTTTCTAA CTTATCCAAA ACAACTTTCC ACTGTTGCTT TCGGAGTGAG CAAGATAGAA 
     1321 ACTGCTCCTT ATGTGCAGAC AACATTGAAG GAAAGACATT TGTTTCAACA GTAAATTCTT 
     1381 TAGTTTTTCA ACAAATAGAT GCAAACTGGA ACATACAGTG CTGGCTAAAA GGAGACTTAA 
     1441 AATTATTCAT CTGTTATGTG GAGTCATTAT TTAAGAATCT ATTCAGGAAT TATAACTATA 
     1501 AGGTCCATCT TTTATATGTT CTGCCTGAAG TGTTAGAAGA TTCACCTCTG GTTCCCCAAA 
     1561 AAGGCAGTTT TCAGATGGTT CACTGCAATT GCAGTGTTCA TGAATGTTGT GAATGTCTTG 
     1621 TGCCTGTGCC AACAGCCAAA CTCAACGACA CTCTCCTTAT GTGTTTGAAA ATCACATCTG 
     1681 GTGGAGTAAT TTTCCAGTCA CCTCTAATGT CAGTTCAGCC CATAAATATG GTGAAGCCTG 
     1741 ATCCACCATT AGGTTTGCAT ATGGAAATCA CAGATGATGG TAATTTAAAG ATTTCTTGGT 
     1801 CCAGCCCACC ATTGGTACCA TTTCCACTTC AATATCAAGT GAAATATTCA GAGAATTCTA 
     1861 CAACAGTTAT CAGAGAAGCT GACAAGATTG TCTCAGCTAC ATCCCTGCTA GTAGACAGTA 
     1921 TACTTCCTGG GTCTTCGTAT GAGGTTCAGG TGAGGGGCAA GAGACTGGAT GGCCCAGGAA 
     1981 TCTGGAGTGA CTGGAGTACT CCTCGTGTCT TTACCACACA AGATGTCATA TACTTTCCAC 
     2041 CTAAAATTCT GACAAGTGTT GGGTCTAATG TTTCTTTTCA CTGCATCTAT AAGAAGGAAA 
     2101 ACAAGATTGT TCCCTCAAAA GAGATTGTTT GGTGGATGAA TTTAGCTGAG AAAATTCCTC 
     2161 AAAGCCAGTA TGATGTTGTG AGTGATCATG TTAGCAAAGT TACTTTTTTC AATCTGAATG 
     2221 AAACCAAACC TCGAGGAAAG TTTACCTATG ATGCAGTGTA CTGCTGCAAT GAACATGAAT 
     2281 GCCATCATCG CTATGCTGAA TTATATGTGA TTGATGTCAA TATCAATATC TCATGTGAAA 
     2341 CTGATGGGTA CTTAACTAAA ATGACTTGCA GATGGTCAAC CAGTACAATC CAGTCACTTG 
     2401 CGGAAAGCAC TTTGCAATTG AGGTATCATA GGAGCAGCCT TTACTGTTCT GATATTCCAT 
     2461 CTATTCATCC CATATCTGAG CCCAAAGATT GCTATTTGCA GAGTGATGGT TTTTATGAAT 
     2521 GCATTTTCCA GCCAATCTTC CTATTATCTG GCTACACAAT GTGGATTAGG ATCAATCACT 
     2581 CTCTAGGTTC ACTTGACTCT CCACCAACAT GTGTCCTTCC TGATTCTGTG GTGAAGCCAC 
     2641 TGCCTCCATC CAGTGTGAAA GCAGAAATTA CTATAAACAT TGGATTATTG AAAATATCTT 
     2701 GGGAAAAGCC AGTCTTTCCA GAGAATAACC TTCAATTCCA GATTCGCTAT GGTTTAAGTG 
     2761 GAAAAGAAGT ACAATGGAAG ATGTATGAGG TTTATGATGC AAAATCAAAA TCTGTCAGTC 
     2821 TCCCAGTTCC AGACTTGTGT GCAGTCTATG CTGTTCAGGT GCGCTGTAAG AGGCTAGATG 
     2881 GACTGGGATA TTGGAGTAAT TGGAGCAATC CAGCCTACAC AGTTGTCATG GATATAAAAG 
     2941 TTCCTATGAG AGGACCTGAA TTTTGGAGAA TAATTAATGG AGATACTATG AAAAAGGAGA 
     3001 AAAATGTCAC TTTACTTTGG AAGCCCCTGA TGAAAAATGA CTCATTGTGC AGTGTTCAGA 
     3061 GATATGTGAT AAACCATCAT ACTTCCTGCA ATGGAACATG GTCAGAAGAT GTGGGAAATC 
     3121 ACACGAAATT CACTTTCCTG TGGACAGAGC AAGCACATAC TGTTACGGTT CTGGCCATCA 
     3181 ATTCAATTGG TGCTTCTGTT GCAAATTTTA ATTTAACCTT TTCATGGCCT ATGAGCAAAG 
     3241 TAAATATCGT GCAGTCACTC AGTGCTTATC CTTTAAACAG CAGTTGTGTG ATTGTTTCCT 
     3301 GGATACTATC ACCCAGTGAT TACAAGCTAA TGTATTTTAT TATTGAGTGG AAAAATCTTA 
     3361 ATGAAGATGG TGAAATAAAA TGGCTTAGAA TCTCTTCATC TGTTAAGAAG TATTATATCC 
     3421 ATGATCATTT TATCCCCATT GAGAAGTACC AGTTCAGTCT TTACCCAATA TTTATGGAAG 
     3481 GAGTGGGAAA ACCAAAGATA ATTAATAGTT TCACTCAAGA TGATATTGAA AAACACCAGA 
     3541 GTGATGCAGG TTTATATGTA ATTGTGCCAG TAATTATTTC CTCTTCCATC TTATTGCTTG 
     3601 GAACATTATT AATATCACAC CAAAGAATGA AAAAGCTATT TTGGGAAGAT GTTCCGAACC 
     3661 CCAAGAATTG TTCCTGGGCA CAAGGACTTA ATTTTCAGAA GAAAATGCCT GGCACAAAGG 
     3721 AACTACTGGG TGGAGGTTGG TTGACTACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     3781 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     3841 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     3901 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     3961 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4021 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4081 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4141 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4201 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4261 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4321 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4381 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     4441 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     4501 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     4561 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     4621 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     4681 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     4741 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     4801 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     4861 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     4921 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     4981 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5041 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5101 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5161 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5221 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     5281 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     5341 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     5401 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     5461 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     5521 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     5581 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     5641 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     5701 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     5761 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     5821 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     5881 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     5941 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6001 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6061 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6121 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6181 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6241 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     6301 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     6361 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     6421 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     6481 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     6541 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     6601 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     6661 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     6721 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     6781 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     6841 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     6901 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     6961 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7021 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7081 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7141 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7201 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7261 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     7321 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     7381 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     7441 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     7501 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     7561 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//