LOCUS       RC231432                7527 bp    DNA     circular  SYN 
DEFINITION  TMTC1 (Myc-DDK-tagged)-Human transmembrane and tetratricopeptide repeat containing 1 (TMTC1), transcript variant 1
ACCESSION   NM_001193451
VERSION     NM_001193451.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3684..3775 
                     /label="Myc-DDK"  
     misc_feature    3805..4390 
                     /label="PolyA signal"  
     misc_feature    3862..3881 
                     /label="Restriction Enzyme"  
     misc_feature    4666..5338 
                     /label="ColE1"  
     misc_feature    5768..6562 
                     /label="Kan/Neo"  
     misc_feature    6597..6899 
                     /label="SV40 ori"  
     misc_feature    6985..7462 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3666..3672 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3666 
                     /label="TMTC1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGGTGACC ACCTCTGCCC GAGGCGGCGG CGGGGACCGC ACACCCTCCC 
     1081 GGCGGCGGGG CTGCGGGCTA GCGCCGGCCG GGGCCGCGGC GCTGCTGGCC GGGGCAAGCT 
     1141 GCCTGTGCTA CGGCCGCTCC CTGCAGGGCG AGTTCGTGCA CGACGACGTG TGGGCGATCG 
     1201 TGAACAACCC CGACGTGCGG CCCGGCGCCC CGCTCCGCTG GGGCATCTTC ACCAACGACT 
     1261 TCTGGGGCAA GGGCATGGCC GAGAACACCA GCCACAAGTC CTACCGGCCG CTCTGCGTCC 
     1321 TCACCTTCAA GCTAAACATA TTTTTGACTG GTATGAACCC ATTCTACTTT CATGCAGTAA 
     1381 ATATAATTTT ACACTGCTTA GTGACTCTTG TGCTGATGTA CACCTGTGAT AAAACTGTCT 
     1441 TCAAGAATCG TGGACTTGCT TTTGTAACGG CATTGCTTTT TGCTGTACAT CCTATTCATA 
     1501 CTGAGGCGGT GGCTGGGATC GTTGGCAGAG CGGACGTGTT AGCGTGTCTG CTGTTTCTAT 
     1561 TGGCCTTTCT CTCGTACAAC AGGAGTCTGG ATCAGGGCTG TGTTGGGGGA AGTTTCCCTT 
     1621 CCACGGTGTC TCCCTTCTTC TTGCTGCTCA GTTTGTTTCT GGGGACCTGT GCGATGCTGG 
     1681 TGAAAGAGAC AGGCATCACG GTGTTTGGAG TGTGCTTGGT TTATGACCTC TTTTCCCTTT 
     1741 CCAACAAGCA AGACAAGTCG AGCAATGGGG CCCTCTGTCC ACGCAGCCCA CAGCAGCCCG 
     1801 GGAGCCCCCA GCCCTCCTCA CTGCCAGGCC ATCCTCACCG GGAGAATGGG AAGCAGCAGC 
     1861 GGTTCCCTCA CAAAGGAGCT TGGGGTGGCT GCCACTCTCC ACTGCCACCA GAACCCAAGA 
     1921 GCAGTGGATT CCCAGTGTCC CCACGAGCTG TGTGGTCCAT GATGAGATTC CTCACCTATT 
     1981 CCTACCTCTT GGCCTTCAAT GTGTGGCTTC TGCTTGCACC CGTGACCCTG TGCTATGACT 
     2041 GGCAGGTCGG CAGTATTCCT CTGGTAGAGA CCATATGGGA CATGCGGAAC TTAGCCACCA 
     2101 TCTTTCTGGC GGTTGTGATG GCCTTATTGA GCCTGCACTG CTTAGCAGCC TTTAAGAGAC 
     2161 TGGAGCACAA GGAGGTTTTA GTCGGCTTGT TGTTCCTGGT GTTCCCGTTC ATTCCAGCCA 
     2221 GCAACCTCTT CTTCAGGGTG GGTTTTGTGG TGGCGGAGAG AGTCCTTTAC ATGCCTAGCA 
     2281 TGGGCTACTG CATCCTTTTT GTGCATGGAC TGAGCAAGCT CTGCACTTGG CTGAATCGAT 
     2341 GTGGGGCCAC CACCCTGATT GTGTCCACTG TGTTGCTGCT GTTGCTTTTC TCTTGGAAAA 
     2401 CTGTGAAACA GAATGAAATT TGGCTGTCAA GAGAGTCCCT ATTCAGGTCT GGAGTTCAAA 
     2461 CTCTGCCCCA CAATGCCAAG GTTCACTACA ACTATGCCAA TTTCCTGAAG GACCAAGGTC 
     2521 GGAACAAGGA AGCGATCTAC CACTACAGAA CAGCTCTCAA GTTGTATCCA CGCCATGCAA 
     2581 GTGCGCTCAA CAACCTTGGA ACACTGACGA GAGACACAGC AGAGGCAAAG ATGTACTATC 
     2641 AGAGGGCTCT CCAGCTCCAT CCACAGCATA ACCGGGCTCT TTTCAATCTG GGGAATCTCC 
     2701 TCAAGTCCCA GGAGAAAAAG GAAGAAGCTA TCACCTTACT GAAGGATTCC ATCAAATATG 
     2761 GTCCAGAGTT TGCAGATGCA TATTCAAGCT TAGCTTCGTT ATTGGCTGAG CAGGAGCGGT 
     2821 TTAAAGAAGC TGAAGAAATA TACCAAACTG GAATAAAGAA CTGTCCAGAC AGCTCAGATT 
     2881 TACACAACAA CTATGGGGTT TTCTTAGTTG ATACTGGCTT ACCAGAAAAG GCAGTGGCCC 
     2941 ATTACCAGCA GGCCATCAAA CTTAGCCCCA GTCATCACGT GGCCATGGTG AACTTGGGAA 
     3001 GACTCTACAG GTCACTGGGA GAGAACAGCA TGGCTGAAGA ATGGTACAAG CGCGCCCTGC 
     3061 AGGTGGCACA CAAAGCTGAG ATATTGTCAC CTTTGGGAGC ACTGTATTAC AACACTGGCC 
     3121 GATACGAAGA GGCTTTGCAG ATTTACCAGG AAGCTGCAGC ACTTCAGCCT TCTCAGAGGG 
     3181 AGCTCCGCTT GGCACTGGCT CAGGTTTTGG CCGTGATGGG TCAGACAAAA GAAGCTGAAA 
     3241 AGATGACCAA TCACATTGTG TCAGAGGAGA CCGGATGCCT TGAATGCTAT CGCCTCTTGT 
     3301 CAGCCATCTA TAGCAAGCAG GAGAACCACG ACAAGGCACT TGATGCTATA GACAAGGCTC 
     3361 TCCAGCTGAA ACCAAAGGAC CCAAAAGTCA TTTCTGAACT TTTTTTCACA AAAGGAAACC 
     3421 AATTAAGAGA GCAGAACCTT CTCGACAAAG CTTTTGAGAG CTATAGAGTG GCTGTGCAAC 
     3481 TAAACCCAGA CCAAGCACAG GCCTGGATGA ACATGGGTGG CATCCAACAC ATCAAGGGAA 
     3541 AATATGTGTC TGCAAGAGCT TATTATGAGA GAGCCTTACA GCTGGTTCCA GACAGCAAAC 
     3601 TGCTGAAGGA AAATCTTGCC AAATTGGATC GCCTAGAAAA ACGATTACAA GAAGTTCGAG 
     3661 AAAAGGATCA AACAACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     3721 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     3781 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     3841 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     3901 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     3961 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4021 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4081 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4141 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4201 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4261 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     4321 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     4381 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     4441 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     4501 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     4561 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     4621 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     4681 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     4741 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     4801 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     4861 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     4921 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     4981 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5041 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     5101 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     5161 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     5221 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     5281 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     5341 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     5401 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     5461 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     5521 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     5581 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     5641 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     5701 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     5761 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     5821 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     5881 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     5941 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6001 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6061 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6121 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     6181 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     6241 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     6301 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     6361 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     6421 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     6481 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     6541 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     6601 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     6661 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     6721 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     6781 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     6841 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     6901 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     6961 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7021 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7081 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7141 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     7201 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     7261 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     7321 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     7381 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     7441 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     7501 ATTTAACAAA AATTTAACGC GAATTTT
//