LOCUS       RC231418                7317 bp    DNA     circular  SYN 
DEFINITION  CARS (Myc-DDK-tagged)-Human cysteinyl-tRNA synthetase (CARS), transcript variant 5
ACCESSION   NM_001194997
VERSION     NM_001194997.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3472..3563 
                     /label="Myc-DDK"  
     misc_feature    3593..4178 
                     /label="PolyA signal"  
     misc_feature    3650..3669 
                     /label="Restriction Enzyme"  
     misc_feature    4454..5126 
                     /label="ColE1"  
     misc_feature    5556..6350 
                     /label="Kan/Neo"  
     misc_feature    6385..6687 
                     /label="SV40 ori"  
     misc_feature    6773..7250 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3447..3454 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3447 
                     /label="CARS"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAGATTCC TCCGGGCAGC AGGCTCCTGA CTACAGGTCC ATTCTGAGCA 
     1081 TTAGTGACGA GGCAGCCAGG GCACAAGCCC TGAACGAGCA CCTCAGCACG CGTAGCTATG 
     1141 TCCAGGGGTA CTCACTGTCC CAGGCAGACG TGGACGCGTT CAGGCAGCTC TCGGCCCCGC 
     1201 CCGCTGACCC CCAGCTCTTC CACGTGGCTC GGTGGTTCAG GCACATAGAA GCGCTCCTGG 
     1261 GTAGCCCCTG TGGCAAAGGC CAGCCCTGCA GGCTCCAAGC AAGCAAAGGC CGGCGTGTGC 
     1321 AGCCCCAGTG GTCCCCTCCT GCTGGGACCC AGCCATGCAG ACTCCACCTT TACAACAGCC 
     1381 TCACCAGGAA CAAGGAAGTG TTCATACCTC AAGATGGGAA AAAGGTGACG TGGTATTGCT 
     1441 GTGGGCCAAC CGTCTATGAC GCATCTCACA TGGGGCACGC CAGGTCCTAC ATCTCTTTTG 
     1501 ATATCTTGAG AAGAGTGTTG AAGGATTACT TCAAATTTGA TGTCTTTTAT TGCATGAACA 
     1561 TTACGGATAT TGATGACAAG ATCATCAAGA GGGCCCGGCA GAACCACCTG TTCGAGCAGT 
     1621 ATCGGGAGAA GAGGCCTGAA GCGGCACAGC TCTTGGAGGA TGTTCAGGCC GCCCTGAAGC 
     1681 CATTTTCAGT AAAATTAAAT GAGACCACGG ATCCCGATAA AAAGCAGATG CTCGAACGGA 
     1741 TTCAGCACGC AGTGCAGCTT GCCACAGAGC CACTTGAGAA AGCTGTGCAG TCCAGACTCA 
     1801 CGGGAGAGGA AGTCAACAGC TGTGTGGAGG TGTTGCTGGA AGAAGCCAAG GATTTGCTCT 
     1861 CTGACTGGCT GGATTCTACA CTTGGCTGTG ATGTCACTGA CAATTCCATC TTCTCCAAGC 
     1921 TGCCCAAGTT CTGGGAGGGG GACTTCCACA GAGACATGGA AGCTCTGAAT GTTCTCCCTC 
     1981 CAGATGTCTT AACCCGGGTT AGTGAGTATG TGCCAGAAAT TGTGAACTTT GTCCAGAAGA 
     2041 TTGTGGACAA CGGTTACGGC TATGTCTCCA ATGGGTCTGT CTACTTTGAT ACAGCGAAGT 
     2101 TTGCTTCTAG CGAGAAGCAC TCCTATGGGA AGCTGGTGCC TGAGGCCGTT GGAGATCAGA 
     2161 AAGCCCTTCA AGAAGGGGAA GGTGACCTGA GCATCTCTGC AGACCGCCTG AGTGAGAAGC 
     2221 GCTCTCCCAA CGACTTTGCC TTATGGAAGG CCTCTAAGCC CGGAGAACCG TCCTGGCCGT 
     2281 GCCCTTGGGG AAAGGGTCGT CCGGGCTGGC ATATCGAGTG CTCGGCCATG GCAGGCACCC 
     2341 TCCTAGGGGC TTCGATGGAC ATTCACGGAG GTGGGTTCGA CCTCCGGTTC CCCCACCATG 
     2401 ACAATGAGCT GGCACAGTCG GAGGCCTACT TTGAAAACGA CTGCTGGGTC AGGTACTTCC 
     2461 TGCACACAGG CCACCTGACC ATTGCAGGCT GCAAAATGTC AAAGTCACTA AAAAACTTCA 
     2521 TCACCATTAA AGATGCCTTG AAAAAGCACT CAGCACGGCA GTTGCGGCTG GCCTTCCTCA 
     2581 TGCACTCGTG GAAGGACACC CTGGACTACT CCAGCAACAC CATGGAGTCA GCGCTTCAAT 
     2641 ATGAGAAGTT CTTGAATGAG TTTTTCTTAA ATGTGAAAGA TATCCTTCGC GCTCCTGTTG 
     2701 ACATCACTGG TCAGTTTGAG AAGTGGGGAG AAGAAGAAGC AGAACTGAAT AAGAACTTTT 
     2761 ATGACAAGAA GACAGCAATT CACAAAGCCC TCTGTGACAA TGTTGACACC CGCACCGTCA 
     2821 TGGAAGAGAT GCGGGCCTTG GTCAGTCAGT GCAACCTCTA TATGGCAGCC CGGAAAGCCG 
     2881 TGAGGAAGAG GCCCAACCAG GCTCTGCTGG AGAACATCGC CCTGTACCTC ACCCATATGC 
     2941 TGAAGATCTT TGGGGCCGTA GAAGAGGACA GCTCCCTGGG ATTCCCGGTC GGAGGGCCTG 
     3001 GAACCAGCCT CAGTCTCGAG GCCACAGTCA TGCCCTACCT TCAGGTGTTA TCAGAATTCC 
     3061 GAGAAGGAGT GCGGAAGATT GCCCGAGAGC AAAAAGTCCC TGAGATTCTG CAGCTCAGCG 
     3121 ATGCCCTGCG GGACAACATC CTGCCCGAGC TTGGGGTGCG GTTTGAAGAC CACGAAGGAC 
     3181 TGCCCACAGT GGTGAAACTG GTAGACAGAA ACACCTTATT AAAAGAGAGA GAAGAAAAGA 
     3241 GACGGGTTGA AGAGGAGAAG AGGAAGAAGA AAGAGGAGGC GGCCCGGAGG AAACAGGAAC 
     3301 AAGAAGCAGC AAAGCTGGCC AAGATGAAGA TTCCCCCCAG TGAGATGTTC TTGTCAGAAA 
     3361 CCGACAAATA CTCCAAGTTT GATGAAAATG TAAGCATGGT CTGCCCACAC ATGACATGGA 
     3421 GGGCAAAGAG CTCAGCAAAG GGCAAGCCAA GAAGCAGCGG ACCGACGCGT ACGCGGCCGC 
     3481 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     3541 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     3601 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     3661 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     3721 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     3781 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     3841 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     3901 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     3961 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4021 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4081 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4141 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4201 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     4261 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     4321 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     4381 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     4441 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     4501 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     4561 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     4621 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     4681 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     4741 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     4801 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     4861 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     4921 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     4981 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5041 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     5101 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     5161 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     5221 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     5281 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     5341 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     5401 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     5461 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     5521 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     5581 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     5641 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     5701 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     5761 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     5821 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     5881 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     5941 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6001 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6061 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     6121 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     6181 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     6241 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     6301 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     6361 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     6421 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     6481 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     6541 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6601 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     6661 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     6721 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     6781 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     6841 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     6901 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     6961 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7021 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7081 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     7141 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     7201 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     7261 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//