LOCUS       RC231408                7188 bp    DNA     circular  SYN 
DEFINITION  PDE1C (Myc-DDK-tagged)-Human phosphodiesterase 1C, calmodulin-dependent 70kDa (PDE1C), transcript variant 3
ACCESSION   NM_001191058
VERSION     NM_001191058.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3345..3436 
                     /label="Myc-DDK"  
     misc_feature    3466..4051 
                     /label="PolyA signal"  
     misc_feature    3523..3542 
                     /label="Restriction Enzyme"  
     misc_feature    4327..4999 
                     /label="ColE1"  
     misc_feature    5429..6223 
                     /label="Kan/Neo"  
     misc_feature    6258..6560 
                     /label="SV40 ori"  
     misc_feature    6646..7123 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3327..3333 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3327 
                     /label="PDE1C"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACGGACGCC GGCAACAGGA AGGAGGGCTT CAAAAAATGC CGGAGCGCCA 
     1081 CTTTCAGCAT CGATGGCTAC AGCTTCACCA TAGTTGCAAA TGAAGCTGGA GACAAGAATG 
     1141 CCAGACCACT GGCCCGCTTC TCTCGGTCTA AGTCACAGAA CTGTCTGTGG AACTCCCTCA 
     1201 TCGATGGGCT CACAGGGAAT GTCAAGGAGA AGCCAAGGCC AACAATTGTC CATGACCCTC 
     1261 GACCCCCAGA GGAGATCCTA GCTGATGAAT TGCCACAGCT GGATAGCTCA GAAGTCTTGG 
     1321 TGAAGACATC CTTCAGATTA CGGTCTTTGG TCAAACAATT AGAGAGAGGG GAAGCTTCAG 
     1381 TGGTAGATCT TAAGAAGAAT TTGGAATATG CAGCCACAGT GCTTGAATCT GTGTATATTG 
     1441 ATGAAACAAG GAGACTCCTG GATACAGAGG ATGAGCTCAG TGACATTCAG TCAGATGCTG 
     1501 TGCCTTCTGA GGTCCGAGAC TGGCTGGCCT CCACCTTCAC GCGGCAGATG GGGATGATGC 
     1561 TCAGGAGGAG CGACGAGAAG CCCCGGTTCA AGAGCATCGT TCACGCAGTG CAGGCTGGGA 
     1621 TATTTGTGGA GAGAATGTAT AGACGGACAT CAAACATGGT TGGACTGAGC TATCCACCAG 
     1681 CTGTTATTGA GGCATTAAAG GATGTGGACA AGTGGTCCTT TGACGTCTTT TCCCTCAATG 
     1741 AGGCCAGTGG GGATCATGCA CTGAAATTTA TTTTCTATGA ACTACTCACA CGTTATGATC 
     1801 TGATCAGCCG TTTCAAGATC CCCATTTCTG CACTTGTCTC ATTTGTGGAG GCCCTGGAAG 
     1861 TGGGATACAG CAAGCACAAA AATCCTTACC ATAACTTAAT GCACGCTGCC GATGTTACAC 
     1921 AGACAGTGCA TTACCTCCTC TATAAGACAG GAGTGGCGAA CTGGCTGACG GAGCTGGAGA 
     1981 TCTTTGCTAT AATCTTCTCA GCTGCCATCC ATGACTACGA GCATACCGGA ACCACCAACA 
     2041 ATTTCCACAT TCAGACTCGG TCTGATCCAG CTATTCTGTA TAATGACAGA TCTGTACTGG 
     2101 AGAATCACCA TTTAAGTGCA GCTTATCGCC TTCTGCAAGA TGACGAGGAA ATGAATATTT 
     2161 TGATTAACCT CTCAAAGGAT GACTGGAGGG AGTTTCGAAC CTTGGTAATT GAAATGGTGA 
     2221 TGGCCACAGA TATGTCTTGT CACTTCCAAC AAATCAAAGC AATGAAGACT GCTCTGCAGC 
     2281 AGCCAGAAGC CATTGAAAAG CCAAAAGCCT TATCCCTTAT GCTGCATACA GCAGATATTA 
     2341 GCCATCCAGC AAAAGCATGG GACCTCCATC ATCGCTGGAC AATGTCACTC CTGGAGGAGT 
     2401 TCTTCAGACA GGGTGACAGA GAAGCAGAGC TGGGGCTGCC TTTTTCTCCT CTGTGTGACC 
     2461 GAAAGTCCAC TATGGTTGCT CAGTCACAAG TAGGTTTCAT TGATTTCATC GTGGAACCCA 
     2521 CCTTCACTGT GCTTACGGAC ATGACCGAGA AGATTGTGAG TCCATTAATC GATGAAACCT 
     2581 CTCAAACTGG TGGGACAGGA CAGAGGCGTT CGAGTTTGAA TAGCATCAGC TCGTCAGATG 
     2641 CCAAGCGATC AGGTGTCAAG ACCTCTGGTT CAGAGGGAAG TGCCCCGATC AACAATTCTG 
     2701 TCATCTCCGT TGACTATAAG AGCTTTAAAG CTACTTGGAC GGAAGTGGTG CACATCAATC 
     2761 GGGAGAGATG GAGGGCCAAG GTACCCAAAG AGGAGAAGGC CAAGAAGGAA GCAGAGGAAA 
     2821 AGGCTCGCCT GGCCGCAGAG GAGCAGCAAA AGGAAATGGA AGCCAAAAGC CAGGCTGAAG 
     2881 AAGGCGCATC TGGCAAAGCT GAGAAAAAGA CGTCTGGAGA AACTAAGAAT CAAGTCAATG 
     2941 GAACACGGGC AAACAAAAGT GACAACCCTC GTGGGAAAAA CTCCAAAGCC GAGAAGTCAT 
     3001 CAGGAGAACA GCAACAGAAT GGTGACTTCA AAGATGGTAA AAATAAGACA GACAAGAAGG 
     3061 ATCACTCTAA CATCGGAAAT GATTCAAAGA AAACAGATGG CACAAAACAG CGTTCTCACG 
     3121 GCTCACCAGC CCCAAGCACC AGCTCCACGT GTCGCCTTAC GTTGCCAGTC ATCAAGCCTC 
     3181 CTTTGCGTCA TTTTAAACGC CCTGCTTACG CATCTAGCTC CTATGCACCT TCAGTCTCAA 
     3241 AGAAAACTGA TGAGCATCCT GCAAGGTACA AGATGCTGGA TCAGAGGATC AAAATGAAAA 
     3301 AGATTCAGAA CATCTCACAT AACTGGAACA GAAAAACGCG TACGCGGCCG CTCGAGCAGA 
     3361 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     3421 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     3481 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     3541 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     3601 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     3661 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     3721 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     3781 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     3841 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     3901 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     3961 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4021 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4081 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4141 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     4201 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     4261 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     4321 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     4381 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     4441 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     4501 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     4561 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     4621 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     4681 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     4741 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     4801 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     4861 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     4921 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     4981 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     5041 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5101 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     5161 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     5221 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     5281 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     5341 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     5401 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     5461 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     5521 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     5581 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     5641 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     5701 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     5761 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     5821 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     5881 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     5941 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6001 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6061 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6121 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     6181 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     6241 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     6301 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     6361 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     6421 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6481 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     6541 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     6601 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     6661 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     6721 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     6781 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     6841 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     6901 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     6961 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7021 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7081 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7141 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//