LOCUS       RC231393                7053 bp    DNA     circular  SYN 
DEFINITION  DTNA (Myc-DDK-tagged)-Human dystrobrevin, alpha (DTNA), transcript variant 10
ACCESSION   NM_001198938
VERSION     NM_001198938.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3210..3301 
                     /label="Myc-DDK"  
     misc_feature    3331..3916 
                     /label="PolyA signal"  
     misc_feature    3388..3407 
                     /label="Restriction Enzyme"  
     misc_feature    4192..4864 
                     /label="ColE1"  
     misc_feature    5294..6088 
                     /label="Kan/Neo"  
     misc_feature    6123..6425 
                     /label="SV40 ori"  
     misc_feature    6511..6988 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3192..3198 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3192 
                     /label="DTNA"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATTGAAGAT AGTGGGAAAA GAGGAAATAC CATGGCAGAA AGAAGACAGC 
     1081 TGTTTGCAGA GATGAGGGCT CAAGATCTGG ATCGCATCCG ACTCTCCACC TACAGAACAG 
     1141 CATGCAAGCT TAGGTTTGTT CAGAAGAAAT GCAATTTGCA CCTGGTGGAC ATATGGAATG 
     1201 TCATAGAAGC ATTGCGGGAA AATGCTCTGA ACAACCTGGA CCCAAACACT GAACTCAACG 
     1261 TGTCCCGCTT AGAGGCTGTG CTCTCCACTA TTTTTTACCA GCTCAACAAA CGGATGCCAA 
     1321 CCACTCACCA AATCCATGTG GAGCAGTCCA TCAGCCTCCT CCTTAACTTC CTGCTTGCAG 
     1381 CGTTTGATCC GGAAGGCCAT GGTAAAATTT CAGTATTTGC TGTCAAAATG GCTTTAGCCA 
     1441 CATTGTGTGG AGGGAAGATC ATGGACAAAT TAAGATATAT TTTCTCAATG ATTTCTGACT 
     1501 CCAGTGGGGT GATGGTTTAT GGACGATATG ACCAATTCCT TCGGGAAGTT CTCAAACTAC 
     1561 CCACGGCAGT TTTTGAAGGT CCTTCATTTG GTTACACAGA ACAGTCAGCC AGATCCTGTT 
     1621 TCTCCCAACA GAAAAAAGTC ACGTTAAATG GTTTCTTGGA CACGCTTATG TCAGATCCTC 
     1681 CCCCGCAGTG TCTGGTCTGG TTGCCTCTTC TGCATCGACT AGCAAATGTG GAAAATGTCT 
     1741 TCCATCCGGT TGAGTGTTCC TACTGCCACA GTGAGAGTAT GATGGGATTT CGCTACCGAT 
     1801 GCCAACAGTG TCACAATTAC CAGCTCTGTC AGGACTGCTT CTGGAGGGGA CATGCCGGTG 
     1861 GTTCTCATAG CAACCAGCAC CAAATGAAAG AGTACACGTC ATGGAAATCA CCTGCTAAGA 
     1921 AGCTGACTAA TGCATTAAGC AAGTCCCTGA GCTGTGCTTC CAGCCGTGAA CCTTTGCACC 
     1981 CCATGTTCCC AGATCAGCCT GAGAAGCCAC TCAACTTGGC TCACATCGTG CCTCCCAGAC 
     2041 CTGTAACCAG CATGAACGAC ACCCTGTTCT CCCACTCTGT TCCCTCCTCA GGAAGTCCTT 
     2101 TTATTACCAG GAGCATGCTT GAGAGTTCAA ACCGGCTTGA TGAAGAACAC AGGCTAATTG 
     2161 CCAGGTATGC GGCAAGGCTG GCAGCAGAGT CCTCTTCGTC TCAGCCACCT CAGCAGAGAA 
     2221 GTGCTCCTGA CATCTCTTTC ACCATCGATG CGAATAAGCA GCAAAGGCAG CTGATTGCTG 
     2281 AGCTAGAAAA CAAGAACAGA GAAATCTTAC AGGAGATCCA GAGACTTCGG CTAGAGCATG 
     2341 AACAAGCTTC TCAGCCCACG CCAGAGAAGG CACAGCAAAA CCCCACCCTG CTGGCAGAAC 
     2401 TCCGGCTCCT CAGACAGCGC AAAGATGAGC TGGAACAGAG AATGTCTGCT CTCCAGGAGA 
     2461 GCCGGAGAGA GCTAATGGTC CAGTTGGAGG GTCTCATGAA GCTACTAAAG ACTCAGGGGG 
     2521 CAGGCTCTCC CCGCTCCTCC CCCAGCCACA CCATCAGCAG GCCAATTCCC ATGCCCATCC 
     2581 GGTCAGCGTC AGCCTGCTCC ACCCCGACGC ACACGCCGCA GGACTCCCTC ACAGGAGTAG 
     2641 GGGGAGATGT ACAAGAGGCA TTTGCACAAA GTTCAAGAAG AAACTTAAGG AATGACTTGC 
     2701 TAGTGGCTGC AGATTCCATC ACTAACACTA TGTCCTCTCT TGTGAAAGAG CTGAATTCTG 
     2761 AGGTTGGGAG TGAAACAGAG AGTAATGTGG ATTCTGAATT TGCACGGACT CAGTTTGAGG 
     2821 ATCTTGTTCC CTCACCAACC TCTGAAAAGG CTTTTCTAGC GCAAATCCAT GCCCGAAAAC 
     2881 CTGGGTACAT TCACAGTGGA GCTACCACAA GTACCATGCG TGGCGACATG GTTACGGAGG 
     2941 ATGCAGATCC CTATGTGCAG CCTGAAGATG AAAACTATGA AAATGACTCT GTCCGGCAGC 
     3001 TGGAGAATGA GCTCCAGATG GAGGAATACC TGAAACAGAA GCTGCAAGAT GAAGCTTATC 
     3061 AGCTCCACGT CAGCACTGAG ACCAGACTCG AGCACCCCTG TCCTGTAAGC GAGACAAAAT 
     3121 GGCGTGTGTT ATTTTGGGGT TTTGTGTTTT TTGGTGGGTT TCTTTCCTTG GCTCTCCAGA 
     3181 TTTACTTTTG GGGCCTGTTC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     3241 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     3301 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     3361 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     3421 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     3481 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     3541 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     3601 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     3661 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     3721 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     3781 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     3841 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     3901 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     3961 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     4021 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     4081 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     4141 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     4201 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     4261 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     4321 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     4381 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     4441 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     4501 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     4561 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     4621 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     4681 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     4741 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     4801 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     4861 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     4921 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     4981 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     5041 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     5101 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     5161 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     5221 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     5281 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     5341 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     5401 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     5461 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     5521 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     5581 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     5641 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     5701 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     5761 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     5821 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     5881 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     5941 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     6001 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     6061 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     6121 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     6181 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     6241 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     6301 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6361 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6421 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     6481 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     6541 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     6601 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     6661 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     6721 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     6781 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     6841 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     6901 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     6961 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     7021 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//