LOCUS       RC231341                6756 bp    DNA     circular  SYN 
DEFINITION  ARPC4 (Myc-DDK-tagged)-Human ARPC4-TTLL3 readthrough (ARPC4-TTLL3)
ACCESSION   NM_001198793
VERSION     NM_001198793.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2913..3004 
                     /label="Myc-DDK"  
     misc_feature    3034..3619 
                     /label="PolyA signal"  
     misc_feature    3091..3110 
                     /label="Restriction Enzyme"  
     misc_feature    3895..4567 
                     /label="ColE1"  
     misc_feature    4997..5791 
                     /label="Kan/Neo"  
     misc_feature    5826..6128 
                     /label="SV40 ori"  
     misc_feature    6214..6691 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2895..2901 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2895 
                     /label="ARPC4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACTGCCACT CTCCGCCCCT ACCTGAGTGC CGTGCGGGCC ACATTGCAGG 
     1081 CTGCCCTCTG CCTGGAGAAC TTCTCCTCCC AGGTTGTGGA ACGACACAAC AAGCCGGAAG 
     1141 TGGAAGTCAG GAGTAGCAAA GAGCTCCTGT TACAACCTGT GACCATCAGC AGGAATGAGA 
     1201 AGGAAAAGGT TCTGATTGAG GGCTCCATCA ACTCTGTCCG GGTCAGCATT GCTGTGAAAC 
     1261 AGGCTGATGA GATCGAGAAG ATTTTGTGCC ACAAGTTCAT GCGCTTCATG ATGATGCGAG 
     1321 CAGAGAACTT CTTTATCCTT CGAAGGAAGC CTGTGGAGCA GAAGAAGATC TTTACAATCC 
     1381 AAGGCTGCTA CCCGGTGATC CGGTGTCTCT TGCGCCGGAG GGGCTGGGTG GAGAAGAAGA 
     1441 TGGTCCATCG CTCAGGCCCC ACCCTGCTGC CACCCCAGAA GGATCTGGAT AGCTCAGCGA 
     1501 TGGGTGACAG TGACACCACT GAGGATGAGG ATGAAGATGA GGACGAGGAG TTCCAGCCAT 
     1561 CACAGCTGTT CGACTTCGAT GATTTACTGA AATTTGATGA CCTAGATGGA ACACATGCTC 
     1621 TGATGGTGGG TCTATGTCTC AATCTCCGGA ATTTGCCGTG GTTTGATGAG GTTGATGCCA 
     1681 ACTCCTTCTT CCCACGCTGC TACTGCCTGG GGGCTGAGGA TGACAAAAAA GCCTTCATAG 
     1741 GAGACAAGCA GCCCAAGAAA CAGGAGAAAA ACCCAGTGTT GGTGTCCCCA GAGTTTGTGG 
     1801 ATGAAGCTCT GTGTGCGTGC GAGGAGTACC TTAGCAACTT GGCCCACATG GACATCGACA 
     1861 AGGACCTGGA GGCCCCGCTG TACCTCACCC CCGAGGGCTG GTCCCTCTTC CTCCAGCGCT 
     1921 ACTACCAAGT GGTCCACGAA GGGGCAGAAC TCAGGCACCT CGACACTCAG GTCCAGCGCT 
     1981 GTGAGGACAT CCTGCAGCAG CTGCAGGCCG TGGTACCCCA GATAGACATG GAAGGGGATC 
     2041 GCAACATCTG GATCGTGAAG CCAGGAGCCA AGTCCCGCGG ACGAGGCATC ATGTGCATGG 
     2101 ACCACCTGGA GGAGATGCTG AAGCTGGTGA ACGGCAACCC CGTGGTGATG AAGGACGGCA 
     2161 AGTGGGTGGT GCAGAAGTAT ATTGAGCGGC CCCTCCTCAT CTTTGGCACC AAGTTTGACC 
     2221 TCAGACAGTG GTTCCTGGTA ACTGACTGGA ACCCACTTAC CGTGTGGTTC TACCGCGACA 
     2281 GCTATATCCG CTTTTCCACG CAGCCCTTCT CCCTGAAGAA CCTGGACAAC TCAGTGCACC 
     2341 TGTGCAACAA CTCCATCCAG AAGCACCTGG AGAACTCATG CCATCGGCAT CCACTGCTTC 
     2401 CGCCAGACAA CATGTGGTCT AGCCAGAGGT TCCAGGCCCA CCTGCAGGAG ATGGGTGCCC 
     2461 CAAATGCTTG GTCCACCATC ATCGTGCCTG GCATGAAGGA TGCTGTGATC CACGCACTTC 
     2521 AGACCTCCCA GGACACCGTG CAGTGTCGGA AGGCCAGCTT TGAGCTCTAT GGCGCTGACT 
     2581 TCGTGTTCGG GGAGGACTTC CAGCCCTGGC TGATTGAGAT CAACGCCAGC CCCACGATGG 
     2641 CACCCTCCAC AGCAGTCACT GCCCGGCTCT GTGCTGGCGT GCAAGCTGAC ACCCTGCGCG 
     2701 TGGTCATTGA CCGGATGCTG GACCGCAACT GTGACACAGG AGCCTTTGAG CTCATCTATA 
     2761 AGCAGCCCGT CACCACTTCC CCAGCCTCCA CACCAAGGCC CAGCTGCCTT CTCCCCATGT 
     2821 ACTCCGACAC CAGGGCCAGG TCCTCAGACG ACAGCACAGC AAGCTGGTGG GCACTAAGGC 
     2881 CCTGTCGACC ACAGGCAAGG CCTACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     2941 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     3001 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     3061 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     3121 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     3181 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     3241 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     3301 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     3361 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     3421 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     3481 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     3541 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     3601 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     3661 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     3721 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     3781 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     3841 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     3901 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     3961 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     4021 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     4081 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     4141 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     4201 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     4261 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     4321 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     4381 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     4441 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     4501 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     4561 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     4621 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     4681 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     4741 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     4801 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     4861 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     4921 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     4981 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     5041 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     5101 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     5161 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     5221 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     5281 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     5341 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     5401 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     5461 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     5521 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     5581 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     5641 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     5701 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     5761 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     5821 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     5881 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     5941 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     6001 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6061 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6121 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     6181 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     6241 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     6301 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     6361 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     6421 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     6481 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     6541 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     6601 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     6661 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     6721 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//