LOCUS       RC231328                6654 bp    DNA     circular  SYN 
DEFINITION  NADK (Myc-DDK-tagged)-Human NAD kinase (NADK), transcript variant 3
ACCESSION   NM_001198994
VERSION     NM_001198994.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2811..2902 
                     /label="Myc-DDK"  
     misc_feature    2932..3517 
                     /label="PolyA signal"  
     misc_feature    2989..3008 
                     /label="Restriction Enzyme"  
     misc_feature    3793..4465 
                     /label="ColE1"  
     misc_feature    4895..5689 
                     /label="Kan/Neo"  
     misc_feature    5724..6026 
                     /label="SV40 ori"  
     misc_feature    6112..6589 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2793..2799 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2793 
                     /label="NADK"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAAATGGAA CAAGAAAAAA TGACCATGAA TAAGGAATTG AGTCCAGACG 
     1081 CGGCTGCTTA CTGCTGCTCG GCCTGCCACG GCGATGAGAC CTGGAGTTAC AACCACCCCA 
     1141 TCCGGGGCCG GGCCAAGTCT CGCAGCCTGT CTGCCTCGCC CGCCCTGGGG AGCACCAAGG 
     1201 AGTTCAGGAG GACACGCTCT CTTCATGGGC CATGCCCGGT GACCACTTTT GGACCAAAGG 
     1261 CCTGTGTGCT GCAGAACCCC CAGACCATCA TAGCCACTCC TTTGTCACTC GCATCTCAGC 
     1321 TGCTGCCTTC ACCAGCCGTC AGTCACTCGG GCCAGGGCGG GGTCACCGGA CAGGTGCATG 
     1381 TTCTGCCTCA GCCCTCAGAC CAGGGCGTAC TCAGTGGTCC CCGAGCTGCC CGAGGCCAGA 
     1441 CAGCCCCGCA GGAGGAAGCG GTGACCCAGG AAGAGGTCGA GGCGCTGGTT TGTGGTCACA 
     1501 CACAGCGGTG GGTGCCTGGG CCGGTGTACG ACGCCGCTGC TGGTGGCTCT GGCTGGGCTC 
     1561 AGCTGAGCCT CAGGGCTGGA ATGGGTGTAG GCCAGGCCAC TGGGCACATT CAGGACCCCG 
     1621 CGAGCCAGCG GCTGACGTGG AACAAGTCCC CAAAGAGCGT CCTTGTCATC AAGAAGATGA 
     1681 GAGATGCCAG CCTACTGCAG CCGTTCAAGG AGCTCTGCAC GCACCTCATG GAGGCACGCG 
     1741 GGGCAGGGGG GAAGGGGGCC TGGGGTGCAC ATGGAGTTGG GGGGGCCTCC ATCCACATCA 
     1801 CCGCCCCGAG GGTGGGGTCT GCAGGTGGAA TGAGCAGGTT GGCACTTTGC TTCCAGGAGA 
     1861 ACATGATCGT GTATGTGGAA AAGAAAGTGC TAGAAGACCC TGCCATCGCC AGCGATGAAA 
     1921 GCTTTGGGGC AGTGAAGAAG AAATTCTGTA CCTTTCGAGA AGATTATGAT GACATTTCCA 
     1981 ATCAGATAGA CTTCATCATC TGCCTGGGGG GAGACGGGAC GCTGCTGTAC GCTTCCTCGC 
     2041 TTTTCCAGGG CAGCGTCCCT CCGGTCATGG CCTTCCACCT GGGCTCCCTG GGCTTCCTGA 
     2101 CCCCATTCAG CTTTGAGAAC TTTCAGTCCC AAGTTACTCA GGTGATAGAG GGGAACGCAG 
     2161 CTGTTGTTCT CCGGAGTCGG CTGAAGGTCA GGGTGGTGAA GGAGCTCCGG GGGAAGAAGA 
     2221 CGGCCGTGCA CAATGGGCTG GGTGAGAACG GCTCGCAGGC TGCAGGCCTG GACATGGATG 
     2281 TCGGGAAGCA GGCCATGCAG TACCAGGTCC TGAATGAGGT GGTGATTGAC AGAGGCCCCT 
     2341 CCTCCTACCT GTCCAATGTG GATGTCTACC TGGACGGACA CCTCATCACC ACGGTGCAGG 
     2401 GCGACGGAGT GATCGTGTCC ACCCCGACGG GCAGCACGGC GTATGCGGCC GCGGCCGGGG 
     2461 CCTCCATGAT CCACCCCAAC GTGCCGGCCA TCATGATCAC GCCCATCTGC CCCCACTCGC 
     2521 TGTCCTTCCG GCCCATCGTG GTCCCCGCAG GGGTCGAGCT GAAGATCATG CTGTCACCTG 
     2581 AAGCAAGGAA CACAGCATGG GTGTCCTTTG ATGGACGGAA GAGACAAGAG ATCCGCCATG 
     2641 GAGACAGCAT CAGCATCACT ACCTCATGCT ACCCGCTCCC CTCCATCTGT GTGCGGGACC 
     2701 CCGTGAGCGA CTGGTTTGAG AGCCTCGCCC AGTGCCTGCA TTGGAACGTC CGGAAGAAGC 
     2761 AAGCCCACTT CGAGGAGGAG GAGGAGGAGG AGGAGGAGGG CACGCGTACG CGGCCGCTCG 
     2821 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     2881 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     2941 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     3001 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     3061 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     3121 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     3181 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     3241 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     3301 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     3361 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     3421 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     3481 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     3541 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     3601 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     3661 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     3721 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     3781 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     3841 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     3901 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     3961 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     4021 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     4081 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     4141 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     4201 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     4261 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     4321 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     4381 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     4441 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     4501 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     4561 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     4621 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     4681 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     4741 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     4801 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     4861 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     4921 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     4981 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     5041 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     5101 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     5161 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     5221 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     5281 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     5341 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     5401 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     5461 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     5521 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     5581 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     5641 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     5701 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     5761 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     5821 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     5881 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     5941 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     6001 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     6061 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     6121 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     6181 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     6241 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     6301 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     6361 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     6421 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     6481 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     6541 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     6601 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//