LOCUS       RC230896                9189 bp    DNA     circular  SYN 
DEFINITION  PTPRU (Myc-DDK-tagged)-Human protein tyrosine phosphatase, receptor type, U (PTPRU), transcript variant 4
ACCESSION   NM_001195001
VERSION     NM_001195001.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5344..5435 
                     /label="Myc-DDK"  
     misc_feature    5465..6050 
                     /label="PolyA signal"  
     misc_feature    5522..5541 
                     /label="Restriction Enzyme"  
     misc_feature    6326..6998 
                     /label="ColE1"  
     misc_feature    7428..8222 
                     /label="Kan/Neo"  
     misc_feature    8257..8559 
                     /label="SV40 ori"  
     misc_feature    8645..9122 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5319..5326 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5319 
                     /label="PTPRU"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCGTGCC CAGGCGCTCG TGCTGGCACT CACCTTCCAG CTCTGCGCGC 
     1081 CGGAGACCGA GACTCCGGCA GCTGGCTGCA CCTTCGAGGA GGCAAGTGAC CCAGCAGTGC 
     1141 CCTGCGAGTA CAGCCAGGCC CAGTACGATG ACTTCCAGTG GGAGCAAGTG CGAATCCACC 
     1201 CTGGCACCCG GGCACCTGCG GACCTGCCCC ACGGCTCCTA CTTGATGGTC AACACTTCCC 
     1261 AGCATGCCCC AGGCCAGCGA GCCCATGTCA TCTTCCAGAG CCTGAGCGAG AATGATACCC 
     1321 ACTGTGTGCA GTTCAGCTAC TTCCTGTACA GCCGGGACGG GCACAGCCCG GGCACCCTGG 
     1381 GCGTCTACGT GCGCGTTAAT GGGGGCCCCC TGGGCAGTGC TGTGTGGAAT ATGACTGGAT 
     1441 CCCACGGCCG TCAGTGGCAC CAGGCTGAGC TGGCTGTCAG CACTTTCTGG CCCAATGAAT 
     1501 ATCAGGTGCT GTTTGAGGCC CTCATCTCCC CAGACCGCAG GGGCTACATG GGCCTAGATG 
     1561 ACATCCTGCT TCTCAGCTAC CCCTGCGCAA AGGCCCCACA CTTCTCCCGC CTGGGCGACG 
     1621 TGGAGGTCAA CGCGGGCCAG AACGCGTCGT TCCAGTGCAT GGCCGCGGGC AGAGCGGCCG 
     1681 AGGCCGAACG CTTCCTCTTG CAACGGCAGA GCGGGGCGCT GGTGCCGGCG GCGGGCGTGC 
     1741 GGCACATCAG CCACCGGCGC TTCCTGGCCA CTTTCCCGCT GGCTGCCGTG AGCCGCGCCG 
     1801 AGCAGGACCT GTACCGCTGT GTGTCCCAGG CCCCGCGCGG CGCGGGCGTC TCTAACTTCG 
     1861 CGGAGCTCAT CGTCAAGGAG CCCCCAACTC CCATCGCGCC CCCACAGCTG CTGCGTGCTG 
     1921 GCCCCACCTA CCTCATCATC CAGCTCAACA CCAACTCCAT CATTGGCGAC GGGCCGATCG 
     1981 TGCGCAAGGA GATTGAGTAC CGCATGGCGC GCGGGCCCTG GGCTGAGGTG CACGCCGTCA 
     2041 GCCTGCAGAC CTACAAGCTG TGGCACCTCG ACCCCGACAC AGAGTATGAG ATCAGCGTGC 
     2101 TGCTCACGCG TCCCGGAGAC GGCGGCACTG GCCGCCCTGG GCCACCCCTC ATCAGCCGCA 
     2161 CCAAATGCGC AGAGCCCATG AGGGCCCCCA AAGGCCTGGC TTTTGCTGAG ATCCAGGCCC 
     2221 GTCAGCTGAC CCTGCAGTGG GAACCACTGG GCTACAACGT GACGCGTTGC CACACCTATA 
     2281 CTGTGTCGCT GTGCTATCAC TACACCCTGG GCAGCAGCCA CAACCAGACC ATCCGAGAGT 
     2341 GTGTGAAGAC AGAGCAAGGT GTCAGCCGCT ACACCATCAA GAACCTGCTG CCCTATCGGA 
     2401 ACGTTCACGT GAGGCTTGTC CTCACTAACC CTGAGGGGCG CAAAGAGGGC AAGGAGGTCA 
     2461 CTTTCCAGAC GGATGAGGAT GTGCCCAGTG GGATTGCAGC CGAGTCCCTG ACCTTCACTC 
     2521 CACTGGAGGA CATGATCTTC CTCAAGTGGG AGGAGCCCCA GGAGCCCAAT GGTCTCATCA 
     2581 CCCAGTATGA GATCAGCTAC CAGAGCATCG AGTCATCAGA CCCGGCAGTG AACGTGCCAG 
     2641 GCCCACGACG TACCATCTCC AAGCTCCGCA ATGAGACCTA CCATGTCTTC TCCAACCTGC 
     2701 ACCCAGGCAC CACCTACCTG TTCTCCGTGC GGGCCCGCAC AGGCAAAGGC TTCGGCCAGG 
     2761 CGGCACTCAC TGAGATAACC ACTAACATCT CTGCTCCCAG CTTTGATTAT GCCGACATGC 
     2821 CGTCACCCCT GGGCGAGTCT GAGAACACCA TCACCGTGCT GCTGAGGCCG GCACAGGGCC 
     2881 GCGGTGCGCC CATCAGTGTG TACCAGGTGA TTGTGGAGGA GGAGCGGGCG CGGAGGCTGC 
     2941 GGCGGGAGCC AGGTGGACAG GACTGCTTCC CAGTGCCATT GACCTTCGAG GCGGCGCTGG 
     3001 CCCGAGGCCT GGTGCACTAC TTCGGGGCCG AACTGGCGGC CAGCAGTCTA CCTGAGGCCA 
     3061 TGCCCTTTAC CGTGGGTGAC AACCAGACCT ACCGAGGCTT CTGGAACCCA CCACTTGAGC 
     3121 CTAGGAAGGC CTATCTCATC TACTTCCAGG CAGCAAGCCA CCTGAAGGGG GAGACCCGGC 
     3181 TGAATTGCAT CCGCATTGCC AGGAAAGCTG CCTGCAAGGA AAGCAAGCGG CCCCTGGAGG 
     3241 TGTCCCAGAG ATCGGAGGAG ATGGGGCTTA TCCTGGGCAT CTGTGCAGGG GGGCTTGCTG 
     3301 TCCTCATCCT TCTCCTGGGT GCCATCATTG TCATCATCCG CAAAGGGAAG CCGGTGAACA 
     3361 TGACCAAGGC CACCGTCAAC TACCGCCAGG AGAAGACACA CATGATGAGC GCCGTGGACC 
     3421 GCAGCTTCAC AGACCAGAGC ACCCTGCAGG AGGACGAGCG GCTGGGCCTG TCCTTCATGG 
     3481 ACACCCATGG CTACAGCACC CGGGGAGACC AGCGCAGCGG TGGGGTCACT GAGGCCAGCA 
     3541 GCCTCCTGGG GGGCTCCCCG AGGCGTCCCT GTGGCCGGAA GGGCTCCCCA TATCACACGG 
     3601 GGCAGCTGCA CCCTGCGGTG CGTGTCGCAG ACCTTCTGCA GCACATCAAC CAGATGAAGA 
     3661 CGGCCGAGGG TTACGGCTTC AAGCAGGAGT ATGAGAGCTT CTTTGAAGGC TGGGACGCCA 
     3721 CAAAGAAGAA AGACAAGGTC AAGGGCAGCC GGCAGGAGCC AATGCCTGCC TATGATCGGC 
     3781 ACCGAGTGAA ACTGCACCCG ATGCTGGGAG ACCCCAATGC CGACTACATT AATGCCAACT 
     3841 ACATAGATGG TTACCACAGG TCAAACCACT TCATAGCCAC TCAAGGGCCG AAGCCTGAGA 
     3901 TGGTCTATGA CTTCTGGCGT ATGGTGTGGC AGGAGCACTG TTCCAGCATC GTCATGATCA 
     3961 CCAAGCTGGT CGAGGTGAAA TGCTCACGGT ACTGGCCGGA GGACTCAGAC ACCTACGGGG 
     4021 ACATCAAGAT TATGCTGGTG AAGACAGAGA CCCTGGCTGA GTATGTCGTG CGCACTTTTG 
     4081 CCCTGGAGCG GAGAGGCTAC TCTGCCCGGC ACGAGGTCCG CCAGTTCCAC TTCACAGCGT 
     4141 GGCCAGAGCA TGGCGTCCCC TACCATGCCA CGGGGCTGCT GGCTTTCATC CGGCGCGTGA 
     4201 AGGCCTCCAC CCCACCTGAT GCCGGGCCCA TTGTCATCCA CTGCAGCGCG GGCACCGGCC 
     4261 GCACAGGTTG CTATATCGTC CTGGATGTGA TGCTGGACAT GGCAGAGTGT GAGGGCGTCG 
     4321 TGGACATTTA CAACTGTGTG AAGACTCTCT GCTCCCGGCG TGTCAACATG ATCCAGACTG 
     4381 AGGAGCAGTA CATCTTCATT CATGATGCAA TCCTGGAGGC CTGCCTGTGT GGGGAGACCA 
     4441 CCATCCCTGT CAGTGAGTTC AAGGCCACCT ACAAGGAGAT GATCCGCATT GATCCTCAGA 
     4501 GTAATTCCTC CCAGCTGCGG GAAGAGTTCC AGACGCTGAA CTCGGTCACC CCGCCGCTGG 
     4561 ACGTGGAGGA GTGCAGCATC GCCCTGTTGC CCCGGAACCG CGACAAGAAC CGCAGCATGG 
     4621 ACGTCCTGCC GCCCGACCGC TGCCTGCCCT TCCTCATCTC CACTGATGGG GACTCCAACA 
     4681 ACTACATTAA TGCAGCCCTG ACTGACAGCT ACACACGGAG TGCGGCCTTC ATCGTGACCC 
     4741 TGCACCCGCT GCAGAGCACC ACGCCCGACT TCTGGCGGCT GGTCTACGAT TACGGGTGCA 
     4801 CCTCCATCGT CATGCTCAAC CAGCTGAACC AGTCCAACTC CGCCTGGCCC TGCCTGCAGT 
     4861 ACTGGCCAGA GCCAGGCCGG CAGCAATATG GCCTCATGGA GGTGGAGTTT ATGTCGGGCA 
     4921 CAGCTGATGA AGACTTAGTG GCTCGAGTCT TCCGGGTGCA GAACATCTCT CGGTTGCAGG 
     4981 AGGGGCACCT GCTGGTGCGG CACTTCCAGT TCCTGCGCTG GTCTGCATAC CGGGACACAC 
     5041 CTGACTCCAA GAAGGCCTTC TTGCACCTGC TGGCTGAGGT GGACAAGTGG CAGGCCGAGA 
     5101 GTGGGGATGG GCGCACCATC GTGCACTGCC TAAACGGGGG AGGACGCAGC GGCACCTTCT 
     5161 GCGCCTGCGC CACGGTCCTG GAGATGATCC GCTGCCACAA CTTGGTGGAC GTTTTCTTTG 
     5221 CTGCCAAAAC CCTCCGGAAC TACAAACCCA ACATGGTGGA GACCATGGAT CAGTACCACT 
     5281 TTTGCTACGA TGTGGCCCTG GAGTACTTGG AGGGGCTGGA GTCAAGAAGC GGACCGACGC 
     5341 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     5401 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     5461 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     5521 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5581 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5641 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5701 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5761 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5821 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5881 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5941 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     6001 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     6061 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     6121 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     6181 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     6241 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     6301 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     6361 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     6421 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     6481 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6541 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6601 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6661 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6721 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6781 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6841 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6901 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6961 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     7021 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     7081 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     7141 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     7201 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     7261 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     7321 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     7381 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     7441 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     7501 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7561 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7621 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7681 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7741 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7801 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7861 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7921 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7981 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     8041 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     8101 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     8161 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     8221 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     8281 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     8341 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     8401 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     8461 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     8521 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8581 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8641 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8701 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8761 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8821 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8881 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8941 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     9001 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     9061 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     9121 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     9181 GCGAATTTT
//