LOCUS       RC230895                8706 bp    DNA     circular  SYN 
DEFINITION  CLSPN (Myc-DDK-tagged)-Human claspin (CLSPN), transcript variant 2
ACCESSION   NM_001190481
VERSION     NM_001190481.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4863..4954 
                     /label="Myc-DDK"  
     misc_feature    4984..5569 
                     /label="PolyA signal"  
     misc_feature    5041..5060 
                     /label="Restriction Enzyme"  
     misc_feature    5845..6517 
                     /label="ColE1"  
     misc_feature    6947..7741 
                     /label="Kan/Neo"  
     misc_feature    7776..8078 
                     /label="SV40 ori"  
     misc_feature    8164..8641 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4845..4851 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4845 
                     /label="CLSPN"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACAGGCGAG GTGGGTTCTG AGGTTCACCT AGAAATCAAT GACCCAAACG 
     1081 TCATTTCACA AGAGGAAGCA GATAGTCCTT CAGATAGTGG ACAGGGCAGC TATGAAACAA 
     1141 TTGGACCCTT GAGTGAAGGA GATTCAGATG AAGAGATATT TGTAAGTAAG AAGTTGAAAA 
     1201 ACAGGAAGGT TCTACAAGAC AGTGATTCCG AAACAGAGGA CACAAATGCC TCTCCAGAGA 
     1261 AAACTACCTA TGACAGTGCC GAGGAGGAAA ATAAAGAGAA TTTATATGCT GGGAAAAATA 
     1321 CAAAAATCAA AAGGATTTAC AAAACTGTGG CAGACAGTGA TGAAAGTTAC ATGGAAAAGT 
     1381 CTTTGTATCA GGAAAATCTT GAAGCGCAAG TGAAACCTTG CTTAGAGCTG AGTCTTCAGT 
     1441 CTGGAAACTC TACAGACTTT ACCACTGACA GAAAGAGTTC CAAAAAGCAC ATACATGATA 
     1501 AAGAAGGAAC TGCAGGAAAA GCAAAAGTAA AATCAAAAAG AAGACTTGAG AAAGAGGAGA 
     1561 GAAAAATGGA AAAAATTAGA CAGCTAAAAA AGAAGGAAAC AAAAAACCAG GAAGATGATG 
     1621 TAGAACAGCC ATTTAATGAC AGTGGCTGTC TTCTTGTGGA TAAAGACCTT TTTGAAACTG 
     1681 GGTTGGAGGA TGAAAATAAC TCTCCATTGG AAGATGAAGA GTCATTAGAA TCAATAAGAG 
     1741 CAGCTGTAAA AAACAAAGTA AAAAAGCACA AGAAAAAAGA ACCATCTTTG GAGAGTGGGG 
     1801 TCCATTCATT TGAGGAAGGA AGTGAGTTAT CAAAAGGAAC CACGAGGAAG GAAAGAAAGG 
     1861 CAGCCAGATT AAGTAAAGAA GCATTAAAAC AACTGCATAG TGAGACTCAG CGCCTTATTC 
     1921 GAGAGTCTGC ACTGAACCTT CCATATCATA TGCCTGAGAA TAAAACCATT CATGATTTCT 
     1981 TCAAACGTAA ACCCCGGCCC ACTTGCCACG GAAATGCCAT GGCACTATTG AAGTCATCTA 
     2041 AATATCAGTC AAGCCATCAC AAAGAAATCA TAGACACTGC AAATACTACT GAAATGAACA 
     2101 GTGATCACCA TAGTAAAGGT TCTGAGCAGA CAACAGGTGC AGAAAATGAA GTGGAAACTA 
     2161 ATGCACTCCC TGTAGTTTCA AAGGAAACCC AGATCATTAC TGGATCAGAT GAGTCTTGCA 
     2221 GGAAGGATTT GGTAAAAAAT GAAGAGCTAG AAATTCAGGA GAAACAGAAG CAGAGTGACA 
     2281 TTAGACCTTC ACCTGGGGAC AGCTCAGTGT TGCAACAGGA ATCCAACTTC CTCGGGAACA 
     2341 ATCACAGTGA GGAATGTCAG GTTGGAGGGC TTGTAGCATT TGAACCTCAT GCCCTGGAGG 
     2401 GTGAAGGCCC CCAAAATCCA GAAGAAACAG ATGAGAAAGT GGAAGAGCCT GAGCAGCAAA 
     2461 ATAAATCATC AGCAGTTGGG CCACCTGAAA AAGTGAGACG GTTTACTCTG GATAGACTTA 
     2521 AGCAACTGGG AGTAGATGTT TCCATTAAAC CACGGCTAGG TGCTGATGAA GATTCCTTTG 
     2581 TGATACTTGA ACCTGAAACC AACAGAGGTG AAAAGCTTCA GGTGTTAAAA GCTAAACTGC 
     2641 AAGAAGCAAT GAAACTCCGA AGGTTTGAGG AGCGCCAGAA GCGCCAAGCA CTGTTTAAAT 
     2701 TAGATAATGA AGATGGGTTT GAGGAAGAGG AGGAGGAAGA GGAAGAAATG ACAGATGAGT 
     2761 CTGAGGAAGA TGGAGAAGAG AAGGTAGAGA AAGAAGAGAA AGAGGAAGAA CTAGAGGAAG 
     2821 AGGAGGAGAA AGAAGAGGAG GAGGAAGAAG AAGGAAATCA GGAGACTGCA GAATTCCTTC 
     2881 TTAGTAGTGA AGAAATAGAA ACAAAAGATG AAAAAGAAAT GGATAAAGAA AATAATGATG 
     2941 GCAGTAGTGA AATTGGCAAG GCAGTTGGCT TCCTCTCTGT TCCCAAGTCT CTCTCATCAG 
     3001 ATTCTACTTT ACTTCTGTTT AAGGACAGCT CTTCCAAGAT GGGTTACTTT CCTACTGAAG 
     3061 AAAAATCAGA AACAGATGAA AACTCAGGCA AGCAGCCTAG CAAACTGGAT GAGGATGATT 
     3121 CATGTTCATT GCTAACAAAG GAGAGCAGCC ACAATAGCAG CTTTGAGCTG ATTGGCTCCA 
     3181 CGATTCCATC CTATCAGCCT TGCAACAGAC AAACAGGCCG TGGGACCAGT TTTTTCCCTA 
     3241 CAGCAGGAGG ATTCAGATCT CCTTCCCCTG GGCTATTTCG AGCCAGTTTG GTCAGCTCAG 
     3301 CTTCTAAGAG TTCAGGGAAA CTGTCTGAGC CTTCACTTCC CATAGAGGAT TCCCAGGATC 
     3361 TGTATAACGC CTCCCCAGAG CCTAAGACAC TTTTCCTAGG AGCAGGAGAC TTCCAGTTCT 
     3421 GTTTAGAAGA TGACACTCAG AGCCAACTGT TGGATGCAGA TGGGTTCTTA AATGTTAGAA 
     3481 ACCACAGGAA TCAGTACCAA GCTTTGAAGC CTCGATTGCC ATTGGCCAGT ATGGATGAGA 
     3541 ATGCCATGGA TGCCAACATG GATGAGCTGT TGGATTTGTG TACTGGAAAG TTCACATCTC 
     3601 AGGCTGAAAA ACATCTACCC AGGAAGAGTG ACAAGAAAGA GAACATGGAG GAACTTCTGA 
     3661 ACCTTTGTTC AGGAAAATTC ACTTCTCAGG ATGCCTCCAC TCCAGCCTCA TCAGAGTTAA 
     3721 ATAAACAGGA GAAGGAGAGC AGCATGGGTG ATCCAATGGA AGAAGCACTT GCTCTTTGCT 
     3781 CAGGCTCTTT TCCCACAGAC AAGGAAGAGG AAGACGAGGA GGAGGAATTT GGAGACTTTC 
     3841 GGCTTGTTTC AAATGATAAT GAGTTTGATA GTGATGAGGA TGAACACAGT GACTCTGGTA 
     3901 ATGATCTGGC ACTGGAAGAC CATGAAGATG ATGATGAAGA AGAACTCCTG AAGCGATCTG 
     3961 AGAAGTTGAA AAGGCAAATG AGGTTGAGGA AATACCTGGA GGATGAGGCA GAGGTGTCAG 
     4021 GAAGTGATGT GGGAAGCGAA GATGAGTATG ATGGGGAAGA AATTGATGAA TATGAAGAGG 
     4081 ACGTAATTGA TGAAGTACTT CCTTCTGATG AGGAACTGCA GAGTCAAATC AAGAAAATAC 
     4141 ACATGAAAAC TATGTTGGAT GATGATAAGC GACAGCTACG TTTATACCAA GAGAGGTACC 
     4201 TTGCTGATGG GGATCTGCAC AGCGATGGTC CTGGGCGAAT GAGGAAGTTT CGATGGAAAA 
     4261 ACATAGATGA TGCTTCCCAG ATGGACTTGT TCCACAGAGA CTCTGATGAT GATCAGACTG 
     4321 AAGAACAGCT TGATGAGTCA GAAGCCAGGT GGAGGAAGGA GCGAATTGAA CGAGAGCAGT 
     4381 GGCTTCGGGA CATGGCACAG CAGGGGAAAA TTACAGCTGA AGAAGAAGAA GAAATTGGGG 
     4441 AGGACAGTCA GTTTATGATA CTGGCCAAGA AAGTTACAGC CAAAGCACTG CAGAAGAATG 
     4501 CCAGTCGCCC TATGGTTATT CAGGAATCAA AGTCTTTGCT CAGAAATCCT TTTGAAGCCA 
     4561 TCAGACCAGG AAGTGCTCAA CAGGTGAAGA CAGGCTCACT GCTAAACCAG CCCAAAGCTG 
     4621 TGCTTCAGAA ACTGGCTGCT CTCTCTGACC ATAACCCCAG TGCTCCTCGA AATTCAAGAA 
     4681 ACTTTGTCTT TCATACACTT TCTCCTGTCA AGGCTGAGGC GGCAAAGGAA TCGTCTAAGT 
     4741 CTCAGGTAAA GAAAAGGGGT CCATCTTTCA TGACTTCTCC TTCACCTAAG CACCTCAAAA 
     4801 CAGATGATAG CACTTCAGGA TTGACGCGAA GCATCTTCAA ATATTTGGAG AGCACGCGTA 
     4861 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4921 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4981 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     5041 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     5101 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     5161 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5221 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5281 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5341 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5401 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5461 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5521 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5581 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5641 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5701 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5761 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5821 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5881 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5941 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     6001 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     6061 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     6121 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6181 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6241 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6301 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6361 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6421 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6481 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6541 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6601 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6661 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6721 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6781 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6841 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6901 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6961 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     7021 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     7081 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     7141 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7201 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7261 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7321 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7381 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7441 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7501 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7561 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7621 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7681 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7741 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7801 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7861 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7921 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7981 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     8041 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8101 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     8161 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8221 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8281 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8341 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8401 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8461 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8521 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8581 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8641 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8701 AATTTT
//