LOCUS       RC230894                8457 bp    DNA     circular  SYN 
DEFINITION  NRCAM (Myc-DDK-tagged)-Human neuronal cell adhesion molecule (NRCAM), transcript variant 5
ACCESSION   NM_001193583
VERSION     NM_001193583.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4614..4705 
                     /label="Myc-DDK"  
     misc_feature    4735..5320 
                     /label="PolyA signal"  
     misc_feature    4792..4811 
                     /label="Restriction Enzyme"  
     misc_feature    5596..6268 
                     /label="ColE1"  
     misc_feature    6698..7492 
                     /label="Kan/Neo"  
     misc_feature    7527..7829 
                     /label="SV40 ori"  
     misc_feature    7915..8392 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4596..4602 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4596 
                     /label="NRCAM"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGCTTAAA ATAATGCCGA AAAAGAAGCG CTTATCTGCG GGCAGAGTGC 
     1081 CCCTGATTCT CTTCCTGTGC CAGATGATTA GTGCACTGGA AGTACCTCTT GATCCAAAAC 
     1141 TTCTTGAAGA CTTGGTACAG CCTCCAACCA TCACCCAACA GTCTCCAAAA GATTACATTA 
     1201 TTGACCCTCG GGAGAATATT GTAATCCAGT GTGAAGCCAA AGGGAAACCG CCCCCAAGCT 
     1261 TTTCCTGGAC CCGTAATGGG ACTCATTTTG ACATCGATAA AGACCCTCTG GTCACCATGA 
     1321 AGCCTGGCAC AGGAACGCTC ATAATTAACA TCATGAGCGA AGGGAAAGCT GAGACCTATG 
     1381 AAGGAGTCTA TCAGTGTACA GCAAGGAACG AACGCGGAGC TGCAGTTTCT AATAACATTG 
     1441 TTGTCCGCCC ATCCAGATCA CCATTGTGGA CCAAAGAAAA ACTTGAACCA ATCACACTTC 
     1501 AAAGTGGTCA GTCTTTAGTA CTTCCCTGCA GACCCCCAAT TGGATTACCA CCACCTATAA 
     1561 TATTTTGGAT GGATAATTCC TTTCAAAGAC TTCCACAAAG TGAGAGAGTT TCTCAAGGTT 
     1621 TGAATGGGGA CCTTTATTTT TCCAATGTCC TCCCAGAGGA CACCCGCGAA GACTATATCT 
     1681 GTTATGCTAG ATTTAATCAT ACTCAAACCA TACAGCAGAA GCAACCTATT TCTGTGAAGG 
     1741 TGATTTCAGC TAAATCAAGT AGAGAGAGGC CACCAACATT TTTAACTCCA GAAGGCAATG 
     1801 CAAGTAACAA AGAGGAATTA AGAGGAAATG TGCTTTCACT GGAGTGCATT GCAGAAGGAC 
     1861 TGCCTACCCC AATTATTTAC TGGGCAAAGG AAGATGGAAT GCTACCCAAA AACAGGACAG 
     1921 TTTATAAGAA CTTTGAGAAA ACCTTGCAGA TCATTCATGT TTCAGAAGCA GACTCTGGAA 
     1981 ATTACCAATG TATAGCAAAA AATGCATTAG GAGCCATCCA CCATACCATT TCTGTTAGAG 
     2041 TTAAAGCGGC TCCATACTGG ATCACAGCCC CTCAAAATCT TGTGCTGTCC CCAGGAGAGG 
     2101 ATGGGACCTT GATCTGCAGA GCTAATGGCA ACCCCAAACC CAGAATTAGC TGGTTAACAA 
     2161 ATGGAGTCCC AATAGAAATT GCCCCTGATG ACCCCAGCAG AAAAATAGAT GGCGATACCA 
     2221 TTATTTTTTC AAATGTTCAA GAAAGATCAA GTGCAGTATA TCAGTGCAAT GCCTCTAATG 
     2281 AATATGGATA TTTACTGGCA AACGCATTTG TAAATGTGCT GGCTGAGCCA CCACGAATCC 
     2341 TCACACCTGC AAACACACTC TACCAGGTCA TTGCAAACAG GCCTGCTTTA CTAGACTGTG 
     2401 CCTTCTTTGG GTCTCCTCTC CCAACCATCG AGTGGTTTAA AGGAGCTAAA GGAAGTGCTC 
     2461 TTCATGAAGA TATTTATGTT TTACATGAAA ATGGAACTTT GGAAATTCCT GTGGCCCAAA 
     2521 AGGACAGTAC AGGAACTTAT ACGTGTGTTG CAAGGAATAA ATTAGGGATG GCAAAGAATG 
     2581 AAGTTCACTT AGAAATCAAA GATGCTACAT GGATCGTTAA ACAGCCCGAA TATGCAGTTG 
     2641 TGCAAAGAGG GAGCATGGTG TCCTTTGAAT GCAAAGTGAA ACATGATCAC ACCTTATCCC 
     2701 TCACTGTCCT GTGGCTGAAG GACAACAGGG AACTGCCCAG TGATGAAAGG TTCACTGTTG 
     2761 ACAAGGATCA TCTAGTGGTA GCTGATGTCA GTGACGATGA CAGCGGGACC TACACGTGTG 
     2821 TGGCCAACAC CACTCTGGAC AGCGTCTCCG CCAGCGCTGT GCTTAGCGTT GTTGCTCCTA 
     2881 CTCCAACTCC AGCTCCCGTT TACGATGTCC CAAATCCTCC CTTTGACTTA GAACTGACAG 
     2941 ATCAACTTGA CAAAAGTGTT CAGCTGTCAT GGACCCCAGG CGATGACAAC AATAGCCCCA 
     3001 TTACAAAATT CATCATCGAA TATGAAGATG CAATGCACAA GCCAGGGCTG TGGCACCACC 
     3061 AAACTGAAGT TTCTGGAACA CAGACCACAG CCCAGCTGAA GCTGTCTCCT TACGTGAACT 
     3121 ACTCCTTCCG CGTGATGGCA GTGAACAGCA TTGGGAAGAG CTTGCCCAGC GAGGCCTCTG 
     3181 AGCAGTATTT GACGAAAGCC TCAGAACCAG ATAAAAACCC CACAGCTGTG GAAGGACTGG 
     3241 GATCAGAGCC TGATAATTTG GTGATTACGT GGAAGCCCTT GAATGGTTTC GAATCTAATG 
     3301 GGCCAGGCCT TCAGTACAAA GTTAGCTGGC GCCAGAAAGA TGGTGATGAT GAATGGACAT 
     3361 CTGTGGTTGT GGCAAATGTA TCCAAATATA TTGTCTCAGG CACGCCAACC TTTGTTCCAT 
     3421 ACCTGATCAA AGTTCAGGCC CTGAATGACA TGGGGTTTGC CCCCGAGCCA GCTGTAGTCA 
     3481 TGGGACATTC TGGAGAAGAC CTCCCAATGG TGGCTCCTGG GAACGTGCGT GTGAATGTGG 
     3541 TGAACAGTAC CTTAGCCGAG GTGCACTGGG ACCCAGTACC TCTGAAAAGC ATCCGAGGAC 
     3601 ACCTACAAGG CTATCGGATT TACTATTGGA AGACCCAGAG TTCATCTAAA AGAAACAGAC 
     3661 GTCACATTGA GAAAAAGATC CTCACCTTCC AAGGCAGCAA GACTCATGGC ATGTTGCCGG 
     3721 GGCTAGAGCC CTTTAGCCAC TACACACTGA ATGTCCGAGT GGTCAATGGG AAAGGGGAGG 
     3781 GCCCAGCCAG CCCTGACAGA GTCTTTAATA CTCCAGAAGG AGTCCCCAGT GCTCCCTCGT 
     3841 CTTTGAAGAT TGTGAATCCA ACACTGGACT CTCTCACTTT GGAATGGGAT CCACCGAGCC 
     3901 ACCCGAATGG CATTTTGACA GAGTACACCT TAAAGTATCA GCCAATTAAC AGCACACATG 
     3961 AATTAGGCCC TCTGGTAGAT TTGAAAATTC CTGCCAACAA GACACGGTGG ACTTTAAAAA 
     4021 ATTTAAATTT CAGCACTCGA TATAAGTTTT ATTTCTATGC ACAAACATCA GCAGGATCAG 
     4081 GAAGTCAAAT TACAGAGGAA GCAGTAACAA CTGTGGATGA AGCTGGTATT CTTCCACCTG 
     4141 ATGTAGGTGC AGGCAAAGCG ATGGCAAGCC GGCAGGTGGA TATTGCAACT CAGGGCTGGT 
     4201 TCATTGGTCT GATGTGTGCT GTTGCTCTCC TTATCTTAAT TTTGCTGATT GTTTGCTTCA 
     4261 TCAGAAGAAA CAAGGGTGGT AAATATCCAG TTAAAGAAAA GGAAGATGCC CATGCTGACC 
     4321 CTGAAATCCA GCCTATGAAG GAAGATGATG GGACATTTGG AGAATACAGT GATGCAGAAG 
     4381 ACCACAAGCC TTTGAAAAAA GGAAGTCGAA CTCCTTCAGA CAGGACTGTG AAAAAAGAAG 
     4441 ATAGTGACGA CAGCCTAGTT GACTATGGAG AAGGGGTTAA TGGCCAGTTC AATGAGGATG 
     4501 GCTCCTTTAT TGGACAATAC AGTGGTAAGA AAGAGAAAGA GCCGGCTGAA GGAAACGAAA 
     4561 GCTCAGAGGC ACCTTCTCCT GTCAACGCCA TGAATTCCTT TGTTACGCGT ACGCGGCCGC 
     4621 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4681 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4741 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4801 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4861 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4921 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4981 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5041 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5101 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5161 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5221 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5281 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5341 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5401 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5461 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5521 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5581 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5641 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5701 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5761 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5821 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5881 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5941 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6001 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6061 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6121 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6181 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6241 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6301 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6361 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6421 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6481 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6541 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6601 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6661 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6721 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6781 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6841 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6901 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6961 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7021 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7081 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7141 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7201 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7261 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7321 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7381 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7441 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7501 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7561 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7621 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7681 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7741 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7801 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7861 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7921 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7981 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8041 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8101 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8161 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8221 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8281 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8341 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8401 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//