LOCUS       RC230884                7488 bp    DNA     circular  SYN 
DEFINITION  DNM2 (Myc-DDK-tagged)-Human dynamin 2 (DNM2), transcript variant 5
ACCESSION   NM_001190716
VERSION     NM_001190716.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3645..3736 
                     /label="Myc-DDK"  
     misc_feature    3766..4351 
                     /label="PolyA signal"  
     misc_feature    3823..3842 
                     /label="Restriction Enzyme"  
     misc_feature    4627..5299 
                     /label="ColE1"  
     misc_feature    5729..6523 
                     /label="Kan/Neo"  
     misc_feature    6558..6860 
                     /label="SV40 ori"  
     misc_feature    6946..7423 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3627..3633 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3627 
                     /label="DNM2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCAACCGC GGGATGGAAG AGCTGATCCC GCTGGTCAAC AAACTGCAGG 
     1081 ACGCCTTCAG CTCCATCGGC CAGAGCTGCC ACCTGGACCT GCCGCAGATC GCTGTAGTGG 
     1141 GCGGCCAGAG CGCCGGCAAG AGCTCGGTGC TGGAGAACTT CGTGGGCCGG GACTTCCTTC 
     1201 CCCGCGGTTC AGGAATCGTC ACCCGGCGGC CTCTCATTCT GCAGCTCATC TTCTCAAAAA 
     1261 CAGAACATGC CGAGTTTTTG CACTGCAAGT CCAAAAAGTT TACAGACTTT GATGAAGTCC 
     1321 GGCAGGAGAT TGAAGCAGAG ACCGACAGGG TCACGGGGAC CAACAAAGGC ATCTCCCCAG 
     1381 TGCCCATCAA CCTTCGAGTC TACTCGCCAC ACGTGTTGAA CTTGACCCTC ATCGACCTCC 
     1441 CGGGTATCAC CAAGGTGCCT GTGGGCGACC AGCCTCCAGA CATCGAGTAC CAGATCAAGG 
     1501 ACATGATCCT GCAGTTCATC AGCCGGGAGA GCAGCCTCAT TCTGGCTGTC ACGCCCGCCA 
     1561 ACATGGACCT GGCCAACTCC GACGCCCTCA AGCTGGCCAA GGAAGTCGAT CCCCAAGGCC 
     1621 TACGGACCAT CGGTGTCATC ACCAAGCTTG ACCTGATGGA CGAGGGCACC GACGCCAGGG 
     1681 ACGTCTTGGA GAACAAGTTG CTCCCGTTGA GAAGAGGCTA CATTGGCGTG GTGAACCGCA 
     1741 GCCAGAAGGA TATTGAGGGC AAGAAGGACA TCCGTGCAGC ACTGGCAGCT GAGAGGAAGT 
     1801 TCTTCCTCTC CCACCCGGCC TACCGGCACA TGGCCGACCG CATGGGCACG CCACATCTGC 
     1861 AGAAGACGCT GAATCAGCAA CTGACCAACC ACATCCGGGA GTCGCTGCCG GCCCTACGTA 
     1921 GCAAACTACA GAGCCAGCTG CTGTCCCTGG AGAAGGAGGT GGAGGAGTAC AAGAACTTTC 
     1981 GGCCCGACGA CCCCACCCGC AAAACCAAAG CCCTGCTGCA GATGGTCCAG CAGTTTGGGG 
     2041 TGGATTTTGA GAAGAGGATC GAGGGCTCAG GAGATCAGGT GGACACTCTG GAGCTCTCCG 
     2101 GGGGCGCCCG AATCAATCGC ATCTTCCACG AGCGGTTCCC ATTTGAGCTG GTGAAGATGG 
     2161 AGTTTGACGA GAAGGACTTA CGACGGGAGA TCAGCTATGC CATTAAGAAC ATCCATGGAG 
     2221 TCAGGACCGG GCTTTTCACC CCGGACTTGG CATTCGAGGC CATTGTGAAA AAGCAGGTCG 
     2281 TCAAGCTGAA AGAGCCCTGT CTGAAATGTG TCGACCTGGT TATCCAGGAG CTAATCAATA 
     2341 CAGTTAGGCA GTGTACCAGT AAGCTCAGTT CCTACCCCCG GTTGCGAGAG GAGACAGAGC 
     2401 GAATCGTCAC CACTTACATC CGGGAACGGG AGGGGAGAAC GAAGGACCAG ATTCTTCTGC 
     2461 TGATCGACAT TGAGCAGTCC TACATCAACA CGAACCATGA GGACTTCATC GGGTTTGCCA 
     2521 ATGCCCAGCA GAGGAGCACG CAGCTGAACA AGAAGAGAGC CATCCCCAAT CAGGGGGAGA 
     2581 TCCTGGTGAT CCGCAGGGGC TGGCTGACCA TCAACAACAT CAGCCTGATG AAAGGCGGCT 
     2641 CCAAGGAGTA CTGGTTTGTG CTGACTGCCG AGTCACTGTC CTGGTACAAG GATGAGGAGG 
     2701 AGAAAGAGAA GAAGTACATG CTGCCTCTGG ACAACCTCAA GATCCGTGAT GTGGAGAAGG 
     2761 GCTTCATGTC CAACAAGCAC GTCTTCGCCA TCTTCAACAC GGAGCAGAGA AACGTCTACA 
     2821 AGGACCTGCG GCAGATCGAG CTGGCCTGTG ACTCCCAGGA AGACGTGGAC AGCTGGAAGG 
     2881 CCTCGTTCCT CCGAGCTGGC GTCTACCCCG AGAAGGACCA GGCAGAAAAC GAGGATGGGG 
     2941 CCCAGGAGAA CACCTTCTCC ATGGACCCCC AACTGGAGCG GCAGGTGGAG ACCATTCGCA 
     3001 ACCTGGTGGA CTCATACGTG GCCATCATCA ACAAGTCCAT CCGCGACCTC ATGCCAAAGA 
     3061 CCATCATGCA CCTCATGATC AACAATACGA AGGCCTTCAT CCACCACGAG CTGCTGGCCT 
     3121 ACCTATACTC CTCGGCAGAC CAGAGCAGCC TCATGGAGGA GTCGGCTGAC CAGGCACAGC 
     3181 GGCGGGACGA CATGCTGCGC ATGTACCATG CCCTCAAGGA GGCGCTCAAC ATCATCGGTG 
     3241 ACATCAGCAC CAGCACTGTG TCCACGCCTG TACCCCCGCC TGTCGATGAC ACCTGGCTCC 
     3301 AGAGCGCCAG CAGCCACAGC CCCACTCCAC AGCGCCGACC GGTGTCCAGC ATACACCCCC 
     3361 CTGGCCGGCC CCCAGCAGTG AGGGGCCCCA CTCCAGGGCC CCCCCTGATT CCTGTTCCCG 
     3421 TGGGGGCAGC AGCCTCCTTC TCGGCGCCCC CAATCCCATC CCGGCCTGGA CCCCAGAGCG 
     3481 TGTTTGCCAA CAGTGACCTC TTCCCAGCCC CGCCTCAGAT CCCATCTCGG CCAGTTCGGA 
     3541 TCCCCCCAGG GATTCCCCCA GGAGTGCCCA GAAGACCCCC TGCTGCGCCC AGCCGGCCCA 
     3601 CCATTATCCG CCCAGCCGAG CCATCCCTGC TCGACACGCG TACGCGGCCG CTCGAGCAGA 
     3661 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     3721 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     3781 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     3841 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     3901 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     3961 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4021 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4081 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4141 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4201 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4261 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4321 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4381 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4441 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     4501 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     4561 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     4621 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     4681 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     4741 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     4801 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     4861 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     4921 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     4981 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5041 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5101 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5161 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     5221 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     5281 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     5341 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5401 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     5461 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     5521 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     5581 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     5641 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     5701 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     5761 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     5821 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     5881 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     5941 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6001 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6061 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6121 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6181 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     6241 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6301 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6361 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6421 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     6481 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     6541 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     6601 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     6661 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     6721 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6781 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     6841 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     6901 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     6961 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7021 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7081 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7141 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7201 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     7261 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7321 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7381 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7441 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//