LOCUS       RC230873                7146 bp    DNA     circular  SYN 
DEFINITION  POU2F1 (Myc-DDK-tagged)-Human POU class 2 homeobox 1 (POU2F1), transcript variant 2
ACCESSION   NM_001198783
VERSION     NM_001198783.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3303..3394 
                     /label="Myc-DDK"  
     misc_feature    3424..4009 
                     /label="PolyA signal"  
     misc_feature    3481..3500 
                     /label="Restriction Enzyme"  
     misc_feature    4285..4957 
                     /label="ColE1"  
     misc_feature    5387..6181 
                     /label="Kan/Neo"  
     misc_feature    6216..6518 
                     /label="SV40 ori"  
     misc_feature    6604..7081 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3285..3291 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3285 
                     /label="POU2F1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTGGACTGC AGTGACTATG TTCTAGACTC AAGAATGAAC AATCCGTCAG 
     1081 AAACCAGTAA ACCATCTATG GAGAGTGGAG ATGGCAACAC AGGCACACAA ACCAATGGTC 
     1141 TGGACTTTCA GAAGCAGCCT GTGCCTGTAG GAGGAGCAAT CTCAACAGCC CAGGCGCAGG 
     1201 CTTTCCTTGG ACATCTCCAT CAGGTCCAAC TCGCTGGAAC AAGTTTACAG GCTGCTGCTC 
     1261 AGTCTTTAAA TGTACAGTCT AAATCTAATG AAGAATCGGG GGATTCGCAG CAGCCAAGCC 
     1321 AGCCTTCCCA GCAGCCTTCA GTGCAGGCAG CCATTCCCCA GACCCAGCTT ATGCTAGCTG 
     1381 GAGGACAGAT AACTGGGCTT ACTTTGACGC CTGCCCAGCA ACAGTTACTA CTCCAGCAGG 
     1441 CACAGGCACA GGCACAGCTG CTGGCTGCTG CAGTGCAGCA GCACTCCGCC AGCCAGCAGC 
     1501 ACAGTGCTGC TGGAGCCACC ATCTCCGCCT CTGCTGCCAC GCCCATGACG CAGATCCCCC 
     1561 TGTCTCAGCC CATACAGATC GCACAGGATC TTCAACAACT GCAACAGCTT CAACAGCAGA 
     1621 ATCTCAACCT GCAACAGTTT GTGTTGGTGC ATCCAACCAC CAATTTGCAG CCAGCGCAGT 
     1681 TTATCATCTC ACAGACGCCC CAGGGCCAGC AGGGTCTCCT GCAAGCGCAA AATCTTCTAA 
     1741 CGCAACTACC TCAGCAAAGC CAAGCCAACC TCCTACAGTC GCAGCCAAGC ATCACCCTCA 
     1801 CCTCCCAGCC AGCAACCCCA ACACGCACAA TAGCAGCAAC CCCAATTCAG ACACTTCCAC 
     1861 AGAGCCAGTC AACACCAAAG CGAATTGATA CTCCCAGCTT GGAGGAGCCC AGTGACCTTG 
     1921 AGGAGCTTGA GCAGTTTGCC AAGACCTTCA AACAAAGACG AATCAAACTT GGATTCACTC 
     1981 AGGGTGATGT TGGGCTCGCT ATGGGGAAAC TATATGGAAA TGACTTCAGC CAAACTACCA 
     2041 TCTCTCGATT TGAAGCCTTG AACCTCAGCT TTAAGAACAT GTGCAAGTTG AAGCCACTTT 
     2101 TAGAGAAGTG GCTAAATGAT GCAGAGAACC TCTCATCTGA TTCGTCCCTC TCCAGCCCAA 
     2161 GTGCCCTGAA TTCTCCAGGA ATTGAGGGCT TGAGCCGTAG GAGGAAGAAA CGCACCAGCA 
     2221 TAGAGACCAA CATCCGTGTG GCCTTAGAGA AGAGTTTCTT GGAGAATCAA AAGCCTACCT 
     2281 CGGAAGAGAT CACTATGATT GCTGATCAGC TCAATATGGA AAAAGAGGTG ATTCGTGTTT 
     2341 GGTTCTGTAA CCGCCGCCAG AAAGAAAAAA GAATCAACCC ACCAAGCAGT GGTGGGACCA 
     2401 GCAGCTCACC TATTAAAGCA ATTTTCCCCA GCCCAACTTC ACTGGTGGCG ACCACACCAA 
     2461 GCCTTGTGAC TAGCAGTGCA GCAACTACCC TCACAGTCAG CCCTGTCCTC CCTCTGACCA 
     2521 GTGCTGCTGT GACGAATCTT TCAGTTACAG GCACTTCAGA CACCACCTCC AACAACACAG 
     2581 CAACCGTGAT TTCCACAGCG CCTCCAGCTT CCTCAGCAGT CACGTCCCCC TCTCTGAGTC 
     2641 CCTCCCCTTC TGCCTCAGCC TCCACCTCCG AGGCATCCAG TGCCAGTGAG ACCAGCACAA 
     2701 CACAGACCAC CTCCACTCCT TTGTCCTCCC CTCTTGGGAC CAGCCAGGTG ATGGTGACAG 
     2761 CATCAGGTTT GCAAACAGCA GCAGCTGCTG CCCTTCAAGG AGCTGCACAG TTGCCAGCAA 
     2821 ATGCCAGTCT TGCTGCCATG GCAGCTGCTG CAGGACTAAA CCCAAGCCTG ATGGCACCCT 
     2881 CACAGTTTGC GGCTGGAGGT GCCTTACTCA GTCTGAATCC AGGGACCCTG AGCGGTGCTC 
     2941 TCAGCCCAGC TCTAATGAGC AACAGTACAC TGGCAACTAT TCAAGCTCTT GCTTCTGGTG 
     3001 GCTCTCTTCC AATAACATCA CTTGATGCAA CTGGGAACCT GGTATTTGCC AATGCGGGAG 
     3061 GAGCCCCCAA CATCGTGACT GCCCCTCTGT TCCTGAACCC TCAGAACCTC TCTCTGCTCA 
     3121 CCAGCAACCC TGTTAGCTTG GTCTCTGCCG CCGCAGCATC TGCAGGGAAC TCTGCACCTG 
     3181 TAGCCAGCCT TCACGCCACC TCCACCTCTG CTGAGTCCAT CCAGAACTCT CTCTTCACAG 
     3241 TGGCCTCTGC CAGCGGGGCT GCGTCCACCA CCACCACCGC CTCCAAGGCA CAGACGCGTA 
     3301 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     3361 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     3421 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     3481 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     3541 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     3601 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     3661 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     3721 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     3781 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     3841 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     3901 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     3961 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     4021 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     4081 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     4141 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     4201 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     4261 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     4321 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     4381 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     4441 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     4501 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     4561 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     4621 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     4681 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     4741 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     4801 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     4861 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     4921 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     4981 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     5041 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     5101 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     5161 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     5221 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     5281 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     5341 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     5401 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     5461 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     5521 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     5581 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     5641 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     5701 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     5761 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     5821 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     5881 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     5941 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     6001 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     6061 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     6121 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     6181 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     6241 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     6301 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     6361 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     6421 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     6481 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6541 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     6601 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     6661 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     6721 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     6781 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     6841 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     6901 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     6961 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     7021 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     7081 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     7141 AATTTT
//