LOCUS       RC230718                9252 bp    DNA     circular  SYN 
DEFINITION  ADCY10 (Myc-DDK-tagged)-Human adenylate cyclase 10 (soluble) (ADCY10), transcript variant 2
ACCESSION   NM_001167749
VERSION     NM_001167749.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5409..5500 
                     /label="Myc-DDK"  
     misc_feature    5530..6115 
                     /label="PolyA signal"  
     misc_feature    5587..5606 
                     /label="Restriction Enzyme"  
     misc_feature    6391..7063 
                     /label="ColE1"  
     misc_feature    7493..8287 
                     /label="Kan/Neo"  
     misc_feature    8322..8624 
                     /label="SV40 ori"  
     misc_feature    8710..9187 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5391..5397 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5391 
                     /label="ADCY10"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTGGTCTTT GGAGATGAAA CACACAGCCA CTTTCTGGTG ATTGGTCAGG 
     1081 CAGTGGACGA TGTGCGCCTT GCCCAGAACA TGGCTCAGAT GAATGATGTT ATTCTGTCAC 
     1141 CAAACTGCTG GCAGCTCTGT GACCGGAGCA TGATTGAAAT TGAGAGTGTT CCAGATCAGA 
     1201 GAGCAGTTAA GGTTAACTTC TTAAAACCAC CCCCCAATTT TAATTTTGAT GAATTTTTCA 
     1261 CAAAGTGTAC GACCTTCATG CATTATTATC CTTCTGGTGA GCACAAAAAC CTCCTGAGGC 
     1321 TTGCATGCAC GCTGAAGCCT GATCCTGAAC TGGAGATGTC CCTACAAAAG TATGTGATGG 
     1381 AAAGCATTTT GAAGCAGATT GATAACAAAC AGCTTCAGGG CTATTTATCT GAGCTTCGCC 
     1441 CAGTGACGAT TGTGTTTGTG AACCTGATGT TTGAAGACCA AGACAAAGCA GAAGAGATAG 
     1501 GCCCAGCCAT CCAGGATGCC TATATGCACA TCACTTCTGT CCTGAAGATC TTCCAAGGCC 
     1561 AAATCAATAA AGTCTTCATG TTTGACAAGG GCTGCTCTTT CCTCTGTGTC TTTGGCTTCC 
     1621 CTGGGGAAAA GGTACCTGAC GAGCTCACTC ATGCTCTGGA ATGTGCTATG GATATATTTG 
     1681 ACTTCTGCTC TCAAGTCCAC AAAATCCAAA CTGTATCCAT CGGTGTTGCC AGTGGGATTG 
     1741 TCTTCTGTGG GATCGTTGGA CACACTGTGA GACACGAGTA CACAGTCATT GGTCAAAAAG 
     1801 TCAACTTAGC TGCCAGGATG ATGATGTACT ACCCAGGAAT TGTGACCTGC GACTCTGTCA 
     1861 CCTACAATGG GAGCAACCTA CCAGCGTACT TTTTTAAAGA GCTTCCAAAG AAAGTTATGA 
     1921 AAGGTGTTGC AGATTCTGGA CCATTGTATC AGTATTGGGG CCGTACTGAG AAAGTCATGT 
     1981 TTGGTATGGC GTGCCTCATC TGCAACAGAA AGGAGGATTA CCCTTTGCTG GGACGTAATA 
     2041 AAGAGATCAA CTACTTCATG TATACTATGA AGAAATTTTT GATATCTAAC AGCAGCCAAG 
     2101 TCTTAATGTA TGAGGGATTA CCAGGATATG GAAAAAGCCA GATACTTATG AAAATTGAGT 
     2161 ACCTGGCCCA AGGTAAGAAT CACAGGATTA TTGCCATTTC ATTGAATAAG ATCAGCTTCC 
     2221 ATCAAACTTT CTATACCATC CAGATGTTCA TGGCCAATGT CCTAGGCCTA GACACTTGTA 
     2281 AACATTATAA AGAACGACAG ACCAACCTTC GAAATAAAGT CATGACACTG TTGGATGAAA 
     2341 AGTTCTACTG TCTTCTTAAT GACATTTTCC ATGTTCAGTT CCCTATTTCT CGGGAGATTT 
     2401 CCAGGATGAG CACCTTGAAA AAGCAAAAAC AATTGGAAAT ATTGTTTATG AAGATCTTGA 
     2461 AGCTGATAGT GAAAGAGGAA AGGATTATTT TTATCATTGA TGAGGCCCAG TTTGTGGATT 
     2521 CGACCTCCTG GAGATTTATG GAGAAGCTTA TCCGGACTCT TCCTATCTTC ATCATTATGT 
     2581 CCCTGTGTCC CTTCGTTAAC ATTCCCTGTG CAGCTGCCAG GGCCGTAATA AAGAACAGGA 
     2641 ACACCACCTA CATTGTCATT GGTGCAGTAC AGCCTAACGA CATCTCCAAC AAGATCTGTC 
     2701 TTGACCTCAA TGTGAGCTGC ATCTCCAAAG AACTGGACTC GTACCTGGGG GAGGGAAGCT 
     2761 GTGGGATTCC ATTTTACTGT GAAGAATTGC TTAAAAACCT GGAACATCAT GAGGTACTCG 
     2821 TTTTCCAACA AACGGAGTCT GAGGAAAAGA CAAATAGGAC CTGGAATAAC CTGTTCAAGT 
     2881 ATTCCATTAA GCTAACAGAG AAGTTAAACA TGGTTACTCT CCATAGTGAT AAGGAAAGTG 
     2941 AAGAAGTCTG TCACCTCACA AGTGGTGTCA GACTGAAAAA CCTGTCACCT CCAACGTCAT 
     3001 TAAAAGAAAT CTCTCTGATC CAGCTGGATA GCATGAGACT TTCCCACCAA ATGCTGGTGA 
     3061 GATGTGCTGC CATCATTGGC CTGACCTTCA CCACTGAGTT GTTGTTTGAG ATTCTCCCCT 
     3121 GTTGGAATAT GAAGATGATG ATCAAGACCC TGGCAACCCT AGTGGAATCT AACATTTTTT 
     3181 ATTGTTTCCG GAATGGCAAG GAGCTTCAAA AGGCCCTGAA ACAGAATGAT CCCTCATTTG 
     3241 AGGTGCACTA TCGTTCCTTG TCTCTGAAGC CCAGTGAAGG GATGGATCAC GGTGAAGAGG 
     3301 AACAGCTTCG TGAACTGGAG AATGAGGTGA TCGAGTGCCA CAGGATTCGA TTCTGTAACC 
     3361 CTATGATGCA GAAAACAGCC TACGAGCTGT GGCTCAAGGA CCAGAGAAAA GCCATGCACT 
     3421 TGAAATGTGC CCGCTTTTTA GAAGAAGATG CCCACAGATG TGACCACTGC CGAGGCAGGG 
     3481 ACTTCATTCC CTATCATCAC TTCACAGTGA ATATTCGGCT CAACGCTTTA GACATGGATG 
     3541 CCATTAAAAA GATGGCTATG TCTCATGGAT TTAAAACTGA AGAAAAGCTT ATCTTGTCCA 
     3601 ACTCAGAGAT TCCTGAGACA TCTGCATTTT TTCCTGAAAA TCGCAGTCCT GAAGAAATAA 
     3661 GAGAAAAGAT CTTGAATTTC TTTGACCACG TTTTAACAAA AATGAAGACA TCTGACGAAG 
     3721 ACATTATCCC TCTGGAATCT TGCCAGTGTG AAGAAATCCT AGAGATTGTC ATCTTGCCTC 
     3781 TGGCCCACCA TTTTCTGGCT TTGGGAGAAA ATGACAAAGC CTTATATTAC TTCTTAGAAA 
     3841 TTGCATCTGC TTATCTCATC TTTTGTGATA ACTACATGGC ATACATGTAT TTGAATGAAG 
     3901 GACAGAAGTT GCTAAAAACT CTCAAGAAGG ACAAATCTTG GAGCCAGACA TTTGAGTCTG 
     3961 CCACCTTTTA CAGCCTCAAA GGTGAGGTCT GTTTCAATAT GGGCCAGATA GTGCTTGCCA 
     4021 AGAAAATGCT GAGGAAGGCA CTGAAGCTCC TCAACCGAAT CTTTCCTTAC AACTTAATCT 
     4081 CCTTGTTTCT CCATATCCAT GTCGAGAAAA ACAGACACTT TCATTATGTG AATCGGCAGG 
     4141 CCCAAGAGAG CCCACCTCCA GGGAAGAAGA GGCTGGCACA ACTTTACCGG CAAACTGTCT 
     4201 GCCTTTCCTT GCTGTGGCGC ATCTATAGCT ACAGTTATCT TTTTCACTGC AAGTATTATG 
     4261 CCCACCTGGC AGTTATGATG CAAATGAATA CTGCACTGGA AACTCAAAAT TGTTTCCAGA 
     4321 TCATTAAGGC TTACCTAGAC TATTCGCTAT ACCACCACCT GGCTGGCTAC AAAGGTGTGT 
     4381 GGTTCAAATA TGAAGTCATG GCCATGGAGC ACATCTTCAA CCTCCCCCTG AAAGGCGAGG 
     4441 GCATTGAAAT CGTGGCATAC GTGGCTGAGA CACTGGTCTT CAACAAGCTC ATAATGGGAC 
     4501 ACCTGGATTT GGCCATTGAG TTAGGCTCCC GAGCCCTTCA GATGTGGGCA CTGCTCCAGA 
     4561 ATCCCAACCG ACATTATCAG TCCCTCTGCA GACTTAGCAG ATGTCTCCTT CTGAACAGCA 
     4621 GATACCCGCA ATTGATCCAG GTGCTGGGGC GGCTGTGGGA GCTTTCTGTA ACACAGGAAC 
     4681 ACATCTTCAG CAAGGCATTT TTCTATTTTG TCTGCTTGGA CATCCTGCTT TATTCTGGTT 
     4741 TTGTTTATAG AACATTTGAA GAATGTTTGG AATTCATACA CCAATACGAA AACAACAGAA 
     4801 TCCTCAAGTT CCACAGTGGA CTCCTCCTGG GACTTTATTC CTCTGTAGCT ATCTGGTATG 
     4861 CCAGACTTCA GGAATGGGAC AACTTTTACA AATTTTCCAA TAGAGCTAAA AATCTTTTGC 
     4921 CAAGAAGAAC CATGACACTT ACTTACTATG ACGGAATATC TAGGTACATG GAGGGGCAAG 
     4981 TTCTTCACCT TCAAAAACAA ATCAAAGAAC AGTCAGAGAA TGCCCAAGCC AGTGGGGAGG 
     5041 AGCTACTCAA GAACTTGGAG AATCTGGTGG CTCAAAATAC CACTGGCCCT GTCTTTTGCC 
     5101 CAAGGCTCTA CCACCTGATG GCTTACGTCT GTATATTAAT GGGAGATGGG CAGAAATGTG 
     5161 GCCTCTTCCT GAACACAGCC TTGCGGCTCT CTGAAACACA GGGGAATATA CTGGAGAAAT 
     5221 GCTGGCTGAA CATGAACAAA GAATCATGGT ACTCAACCTC TGAGTTAAAA GAAGACCAAT 
     5281 GGCTTCAGAC GATCTTGAGT CTCCCATCAT GGGAAAAAAT TGTAGCAGGC AGGGTAAACA 
     5341 TTCAGGATCT TCAAAAAAAC AAATTCCTGA TGAGAGCTAA TACCGTGGAC AATCATTTCA 
     5401 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     5461 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     5521 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     5581 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     5641 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     5701 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     5761 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     5821 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     5881 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     5941 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     6001 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     6061 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     6121 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     6181 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     6241 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     6301 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     6361 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     6421 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     6481 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     6541 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     6601 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     6661 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     6721 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     6781 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     6841 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     6901 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6961 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     7021 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     7081 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     7141 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     7201 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     7261 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     7321 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     7381 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     7441 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     7501 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     7561 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     7621 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     7681 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     7741 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     7801 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     7861 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     7921 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7981 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     8041 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     8101 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     8161 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     8221 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     8281 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     8341 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     8401 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     8461 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     8521 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     8581 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8641 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     8701 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     8761 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     8821 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     8881 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     8941 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     9001 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     9061 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     9121 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     9181 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     9241 AACGCGAATT TT
//