LOCUS       RC230713                9105 bp    DNA     circular  SYN 
DEFINITION  NEO1 (Myc-DDK-tagged)-Human neogenin 1 (NEO1), transcript variant 2
ACCESSION   NM_001172623
VERSION     NM_001172623.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5262..5353 
                     /label="Myc-DDK"  
     misc_feature    5383..5968 
                     /label="PolyA signal"  
     misc_feature    5440..5459 
                     /label="Restriction Enzyme"  
     misc_feature    6244..6916 
                     /label="ColE1"  
     misc_feature    7346..8140 
                     /label="Kan/Neo"  
     misc_feature    8175..8477 
                     /label="SV40 ori"  
     misc_feature    8563..9040 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5244..5250 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5244 
                     /label="NEO1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCGGAG CGGGGAGCCC GGCGACTCCT CAGCACCCCC TCCTTCTGGC 
     1081 TCTACTGCCT GCTGCTGCTC GGGCGCCGGG CGCCGGGCGC CGCGGCCGCC AGGAGCGGCT 
     1141 CCGCGCCGCA GTCCCCAGGA GCCAGCATTC GAACGTTCAC TCCATTTTAT TTTCTGGTGG 
     1201 AGCCGGTGGA TACACTCTCA GTTAGAGGCT CTTCTGTTAT ATTAAACTGT TCAGCATATT 
     1261 CTGAGCCTTC TCCAAAAATT GAATGGAAAA AAGATGGAAC TTTTTTAAAC TTAGTATCAG 
     1321 ATGATCGACG CCAGCTTCTC CCGGATGGAT CTTTATTTAT CAGCAATGTG GTGCATTCCA 
     1381 AACACAATAA ACCTGATGAA GGTTATTATC AGTGTGTGGC CACTGTTGAG AGTCTTGGAA 
     1441 CTATTATCAG TAGAACAGCG AAGCTCATAG TAGCAGGTCT TCCAAGATTT ACCAGCCAAC 
     1501 CAGAACCTTC CTCAGTTTAT GCTGGGAACA ATGCAATTCT GAATTGTGAA GTTAATGCAG 
     1561 ATTTGGTCCC ATTTGTGAGG TGGGAACAGA ACAGACAACC CCTTCTTCTG GATGATAGAG 
     1621 TTATCAAACT TCCAAGTGGA ATGCTGGTTA TCAGCAATGC AACTGAAGGA GATGGCGGGC 
     1681 TTTATCGCTG CGTAGTGGAA AGTGGTGGGC CACCAAAGTA TAGTGATGAA GTTGAATTGA 
     1741 AGGTTCTTCC AGATCCTGAG GTGATATCAG ACTTGGTATT TTTGAAACAG CCTTCTCCCT 
     1801 TAGTCAGAGT CATTGGTCAG GATGTAGTGT TGCCATGTGT TGCTTCAGGA CTTCCTACTC 
     1861 CAACCATTAA ATGGATGAAA AATGAGGAGG CACTTGACAC AGAAAGCTCT GAAAGATTGG 
     1921 TATTGCTGGC AGGTGGTAGC CTGGAGATCA GTGATGTTAC TGAGGATGAT GCTGGGACTT 
     1981 ATTTTTGTAT AGCTGATAAT GGAAATGAGA CAATTGAAGC TCAAGCAGAG CTTACAGTGC 
     2041 AAGCTCAACC TGAATTCCTG AAGCAGCCTA CTAATATATA TGCTCACGAA TCTATGGATA 
     2101 TTGTATTTGA ATGTGAAGTG ACTGGAAAAC CAACTCCAAC TGTGAAGTGG GTCAAAAATG 
     2161 GGGATATGGT TATCCCAAGT GATTATTTTA AGATTGTAAA GGAACATAAT CTTCAAGTTT 
     2221 TGGGTCTGGT GAAATCAGAT GAAGGGTTCT ATCAGTGCAT TGCTGAAAAT GATGTTGGAA 
     2281 ATGCACAAGC TGGAGCCCAA CTGATAATCC TTGAACATGC ACCAGCCACA ACGGGACCAC 
     2341 TGCCTTCAGC TCCTCGGGAT GTCGTGGCCT CCCTGGTCTC TACCCGCTTC ATCAAATTGA 
     2401 CGTGGCGGAC ACCTGCATCA GATCCTCACG GAGACAACCT TACCTACTCT GTGTTCTACA 
     2461 CCAAGGAAGG GATTGCTAGG GAACGTGTTG AGAATACCAG TCACCCAGGA GAGATGCAAG 
     2521 TAACCATTCA AAACCTAATG CCAGCGACCG TGTACATCTT TAGAGTTATG GCTCAAAATA 
     2581 AGCATGGCTC AGGAGAGAGT TCAGCTCCAC TGCGAGTAGA AACACAACCT GAGGTTCAGC 
     2641 TCCCTGGCCC AGCACCTAAC CTTCGTGCAT ATGCAGCTTC GCCTACCTCC ATCACTGTTA 
     2701 CGTGGGAAAC ACCAGTGTCT GGCAATGGGG AAATTCAGAA TTATAAATTG TACTACATGG 
     2761 AAAAGGGGAC TGATAAAGAA CAGGATGTTG ATGTTTCAAG TCACTCTTAC ACCATTAATG 
     2821 GGTTGAAAAA ATATACAGAG TATAGTTTCC GAGTGGTGGC CTACAATAAA CATGGTCCTG 
     2881 GAGTTTCCAC ACCAGATGTT GCTGTTCGAA CATTGTCAGA TGTTCCCAGT GCTGCTCCTC 
     2941 AGAATCTGTC CTTGGAAGTG AGAAATTCAA AGAGTATTAT GATTCACTGG CAGCCACCTG 
     3001 CTCCAGCCAC ACAAAATGGG CAGATTACTG GCTACAAGAT TCGCTACCGA AAGGCCTCCC 
     3061 GAAAGAGTGA TGTCACTGAG ACCTTGGTAA GCGGGACACA GCTGTCTCAG CTGATTGAAG 
     3121 GTCTTGATCG GGGGACTGAG TATAATTTCC GAGTGGCTGC TCTAACAATC AATGGTACAG 
     3181 GCCCGGCAAC TGACTGGCTG TCTGCTGAAA CTTTTGAAAG TGACCTAGAT GAAACTCGTG 
     3241 TTCCTGAAGT GCCTAGCTCT CTTCACGTAC GCCCGCTCGT TACTAGCATC GTAGTGAGCT 
     3301 GGACTCCTCC AGAGAATCAG AACATTGTGG TCAGAGGTTA CGCCATTGGT TATGGCATTG 
     3361 GCAGCCCTCA TGCCCAGACC ATCAAAGTGG ACTATAAACA GCGCTATTAC ACCATTGAAA 
     3421 ATCTGGATCC CAGCTCTCAC TATGTGATTA CCCTGAAAGC ATTTAATAAC GTGGGTGAAG 
     3481 GCATCCCCCT GTATGAGAGT GCTGTGACCA GGCCTCACAC AGACACTTCT GAAGTTGATT 
     3541 TATTTGTTAT TAATGCTCCA TACACTCCAG TGCCAGATCC CACTCCCATG ATGCCACCAG 
     3601 TGGGAGTTCA GGCTTCCATT CTGAGTCATG ACACCATCAG GATTACGTGG GCAGACAACT 
     3661 CGCTGCCCAA GCACCAGAAG ATTACAGACT CCCGATACTA CACCGTCCGA TGGAAAACCA 
     3721 ACATCCCAGC AAACACCAAG TACAAGAATG CAAATGCAAC CACTTTGAGT TATTTGGTGA 
     3781 CTGGTTTAAA GCCGAATACA CTCTATGAAT TCTCTGTGAT GGTGACCAAA GGTCGAAGAT 
     3841 CAAGTACATG GAGTATGACA GCCCATGGGA CCACCTTTGA ATTAGTTCCG ACTTCTCCAC 
     3901 CCAAGGATGT GACTGTTGTG AGTAAAGAGG GGAAACCTAA GACCATAATT GTGAATTGGC 
     3961 AGCCTCCCTC CGAAGCCAAT GGCAAAATTA CAGGTTACAT CATATATTAC AGTACAGATG 
     4021 TGAATGCAGA GATACATGAC TGGGTTATTG AGCCTGTTGT GGGAAACAGA CTGACTCACC 
     4081 AGATACAAGA GTTAACTCTT GACACACCAT ACTACTTCAA AATCCAGGCA CGGAACTCAA 
     4141 AGGGCATGGG ACCCATGTCT GAAGCTGTCC AATTCAGAAC ACCTAAAGCG GACTCCTCTG 
     4201 ATAAAATGCC TAATGATCAA GCCTCAGGGT CTGGAGGGAA AGGAAGCCGG CTGCCAGACC 
     4261 TAGGATCCGA CTACAAACCT CCAATGAGCG GCAGTAACAG CCCTCATGGG AGCCCCACCT 
     4321 CTCCTCTGGA CAGTAATATG CTGCTGGTCA TAATTGTTTC TGTTGGCGTC ATCACCATCG 
     4381 TGGTGGTTGT GATTATCGCT GTCTTTTGTA CCCGTCGTAC CACCTCTCAC CAGAAAAAGA 
     4441 AACGAGCTGC CTGCAAATCA GTGAATGGCT CTCATAAGTA CAAAGGGAAT TCCAAAGATG 
     4501 TGAAACCTCC AGATCTCTGG ATCCATCATG AGAGACTGGA GCTGAAACCC ATTGATAAGT 
     4561 CTCCAGACCC AAACCCCATC ATGACTGATA CTCCAATTCC TCGCAACTCT CAAGATATCA 
     4621 CACCAGTTGA CAACTCCATG GACAGCAATA TCCATCAAAG GCGAAATTCA TACAGAGGGC 
     4681 ATGAGTCAGA GGACAGCATG TCTACACTGG CTGGAAGGCG AGGAATGAGA CCAAAAATGA 
     4741 TGATGCCCTT TGACTCCCAG CCACCCCAGC AATCCGTTCG AAATACCCCC AGCACTGACA 
     4801 CCATGCCAGC CTCTTCGTCT CAAACATGCT GCACTGATCA CCAGGACCCT GAAGGTGCTA 
     4861 CCAGCTCCTC TTACTTGGCC AGCTCCCAAG AGGAAGATTC AGGCCAGAGT CTTCCCACTG 
     4921 CCCATGTTCG CCCTTCCCAC CCATTGAAGA GCTTCGCCGT GCCAGCAATC CCGCCTCCAG 
     4981 GACCTCCCAC CTATGATCCT GCATTGCCAA GCACACCATT ACTGTCCCAG CAAGCTCTGA 
     5041 ACCATCACAT TCACTCAGTG AAGACAGCCT CCATCGGGAC TCTAGGAAGG AGCCGGCCTC 
     5101 CTATGCCAGT GGTTGTTCCC AGTGCCCCTG AAGTGCAGGA GACCACAAGG ATGTTGGAAG 
     5161 ACTCCGAGAG TAGCTATGAA CCAGATGAGC TGACCAAAGA GATGGCCCAC CTGGAAGGAC 
     5221 TAATGAAGGA CCTAAACGCT ATCACAACAG CAACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     5281 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     5341 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     5401 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     5461 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5521 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5581 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5641 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5701 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5761 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5821 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5881 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5941 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     6001 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     6061 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     6121 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     6181 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     6241 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     6301 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     6361 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     6421 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6481 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6541 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6601 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6661 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6721 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6781 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6841 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6901 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6961 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     7021 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     7081 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     7141 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     7201 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     7261 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     7321 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     7381 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     7441 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7501 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7561 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7621 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7681 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7741 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7801 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7861 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7921 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7981 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     8041 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     8101 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     8161 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     8221 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     8281 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     8341 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8401 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8461 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8521 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8581 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8641 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8701 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8761 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8821 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8881 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8941 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     9001 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     9061 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//