LOCUS       RC230709                9024 bp    DNA     circular  SYN 
DEFINITION  CDH23 (Myc-DDK-tagged)-Human cadherin-related 23 (CDH23), transcript variant 3
ACCESSION   NM_001171930
VERSION     NM_001171930.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5181..5272 
                     /label="Myc-DDK"  
     misc_feature    5302..5887 
                     /label="PolyA signal"  
     misc_feature    5359..5378 
                     /label="Restriction Enzyme"  
     misc_feature    6163..6835 
                     /label="ColE1"  
     misc_feature    7265..8059 
                     /label="Kan/Neo"  
     misc_feature    8094..8396 
                     /label="SV40 ori"  
     misc_feature    8482..8959 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5163..5169 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5163 
                     /label="CDH23"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGCGCCAT GTTGCCACCA GCTGCCACGT GGCCTGGCTT TTGGTGCTGA 
     1081 TCTCTGGATG CTGGGGCCAG GTGAACCGGC TGCCCTTCTT CACCAACCAC TTCTTTGATA 
     1141 CATACCTGCT GATCAGCGAG GACACGCCTG TGGGTTCTTC TGTGACCCAG TTGCTGGCCC 
     1201 AAGACATGGA CAATGACCCC CTGGTGTTTG GCGTGTCTGG GGAGGAGGCC TCTCGCTTCT 
     1261 TTGCAGTGGA GCCTGACACT GGCGTGGTGT GGCTCCGGCA GCCACTGGAC AGAGAGACCA 
     1321 AGTCAGAGTT CACCGTGGAG TTCTCTGTCA GCGACCACCA GGGGGTGATC ACACGGAAGG 
     1381 TGAACATCCA GGTTGGGGAT GTGAATGACA ACGCGCCCAC ATTTCACAAT CAGCCCTACA 
     1441 GCGTCCGCAT CCCTGAGAAT ACACCAGTGG GGACGCCCAT CTTCATCGTG AATGCCACAG 
     1501 ACCCCGACTT GGGGGCAGGG GGCAGCGTCC TCTACTCCTT CCAGCCCCCC TCCCAATTCT 
     1561 TCGCCATTGA CAGCGCCCGC GGTATCGTCA CAGTGATCCG GGAGCTGGAC TACGAGACCA 
     1621 CACAGGCCTA CCAGCTCACG GTCAACGCCA CAGATCAAGA CAAGACCAGG CCTCTGTCCA 
     1681 CCCTGGCCAA CTTGGCCATC ATCATCACAG ATGTCCAGGA CATGGACCCC ATCTTCATCA 
     1741 ACCTGCCTTA CAGCACCAAC ATCTACGAGC ATTCTCCTCC GGGCACGACG GTGCGCATCA 
     1801 TCACCGCCAT AGACCAGGAT AAAGGACGTC CCCGGGGCAT TGGCTACACC ATCGTTTCAG 
     1861 GGAATACCAA CAGCATCTTT GCCCTGGACT ACATCAGCGG AGTGCTGACC TTGAATGGCC 
     1921 TGCTGGACCG GGAGAACCCC CTGTACAGCC ATGGCTTCAT CCTGACTGTG AAGGGCACGG 
     1981 AGCTGAACGA TGACCGCACC CCATCTGACG CTACAGTCAC CACGACCTTC AATATCCTGG 
     2041 TTATTGACAT CAATGACAAT GCCCCGGAGT TCAACAGCTC CGAGTACAGC GTGGCCATCA 
     2101 CTGAGCTGGC ACAGGTCGGC TTTGCCCTTC CACTCTTCAT CCAGGTGGTG GACAAGGATG 
     2161 AGAATTTGGG CCTGAACAGC ATGTTTGAGG TGTACTTGGT GGGGAACAAC TCCCACCACT 
     2221 TCATCATCTC CCCGACCTCC GTCCAGGGGA AGGCGGACAT TCGTATTCGG GTGGCCATCC 
     2281 CACTGGACTA CGAGACCGTG GACCGCTACG ACTTTGATCT CTTTGCCAAT GAGAGTGTGC 
     2341 CTGACCATGT GGGCTATGCC AAGGTGAAGA TCACTCTCAT CAATGAAAAT GACAACCGGC 
     2401 CCATCTTCAG CCAGCCACTG TACAACATCA GCCTGTACGA GAACGTCACC GTGGGGACCT 
     2461 CTGTGCTGAC AGTCCTGGCA ACTGACAATG ATGCAGGCAC CTTTGGGGAA GTCAGCTACT 
     2521 TCTTCAGTGA TGACCCTGAC AGGTTCTCGC TGGACAAGGA CACGGGACTC ATCATGCTGA 
     2581 TTGCCAGGCT GGACTATGAG CTCATCCAGC GCTTCACCCT GACGATCATT GCCCGGGACG 
     2641 GGGGCGGCGA GGAGACCACA GGCCGGGTCA GGATCAATGT GTTGGATGTC AACGACAACG 
     2701 TGCCCACCTT CCAGAAGGAT GCCTACGTGG GTGCTCTGCG GGAGAACGAG CCTTCTGTCA 
     2761 CACAGCTGGT GCGGCTCCGG GCAACAGATG AAGACTCCCC TCCCAACAAC CAGATCACCT 
     2821 ACAGCATTGT CAGTGCATCT GCCTTTGGCA GCTACTTCGA CATCAGCCTG TACGAGGGCT 
     2881 ATGGAGTGAT CAGCGTCAGT CGCCCCCTGG ATTATGAACA GATATCCAAT GGGCTGATTT 
     2941 ATCTGACGGT CATGGCCATG GATGCTGGCA ACCCCCCTCT CAACAGCACC GTCCCTGTCA 
     3001 CCATCGAGGT GTTTGATGAG AATGACAACC CTCCCACCTT CAGCAAGCCC GCCTACTTCG 
     3061 TCTCCGTGGT GGAGAACATC ATGGCAGGAG CCACGGTGCT GTTCCTGAAT GCCACAGACC 
     3121 TGGACCGCTC CCGGGAGTAC GGCCAGGAGT CCATCATCTA CTCCTTGGAA GGCTCCACCC 
     3181 AGTTTCGGAT CAATGCCCGC TCAGGGGAAA TCACCACCAC GTCTCTGCTT GACCGAGAGA 
     3241 CCAAGTCTGA ATACATCCTC ATCGTTCGCG CAGTGGACGG GGGTGTGGGC CACAACCAGA 
     3301 AAACTGGCAT CGCCACCGTA AACATCACCC TCCTGGACAT CAATGACAAC CACCCCACGT 
     3361 GGAAGGACGC ACCCTACTAC ATCAACCTGG TGGAGATGAC CCCTCCAGAC TCTGATGTGA 
     3421 CCACGGTGGT GGCTGTTGAC CCAGACCTGG GGGAGAATGG CACCCTGGTG TACAGCATCC 
     3481 AGCCACCCAA CAAGTTCTAC AGCCTCAACA GCACCACGGG CAAGATCCGC ACCACCCACG 
     3541 CCATGCTGGA CCGGGAGAAC CCCGACCCCC ATGAGGCCGA GCTGATGCGC AAAATCGTCG 
     3601 TCTCTGTTAC TGACTGTGGC AGGCCCCCTC TGAAAGCCAC CAGCAGTGCC ACAGTGTTTG 
     3661 TGAACCTCTT GGATCTCAAT GACAATGACC CCACCTTTCA GAACCTGCCT TTTGTGGCCG 
     3721 AGGTGCTTGA AGGCATCCCG GCGGGGGTCT CCATCTACCA AGTGGTGGCC ATCGACCTCG 
     3781 ATGAGGGCCT GAACGGCCTG GTGTCCTACC GCATGCCGGT GGGCATGCCC CGCATGGACT 
     3841 TCCTCATCAA CAGCAGCAGC GGCGTGGTGG TCACCACCAC CGAGCTGGAC CGCGAGCGCA 
     3901 TCGCGGAGTA CCAGCTGCGG GTGGTGGCCA GTGATGCAGG CACGCCCACC AAGAGCTCCA 
     3961 CCAGCACGCT CACCATCCAT GTGCTGGATG TGAACGACGA GACGCCCACC TTCTTCCCGG 
     4021 CCGTGTACAA TGTGTCTGTG TCCGAGGACG TGCCACGCGA GTTCCGGGTG GTCTGGCTGA 
     4081 ACTGCACGGA CAACGACGTG GGCCTCAATG CAGAGCTCAG CTACTTCATC ACAGGTGGCA 
     4141 ACGTGGATGG GAAGTTCAGC GTGGGTTACC GCGATGCCGT TGTGAGAACC GTGGTGGGCC 
     4201 TGGACCGGGA GACCACAGCC GCCTACATGC TCATCCTGGA GGCCATCGAC AACGGCCCTG 
     4261 TAGGGAAGCG ACACACGGGC ACAGCCACCG TGTTCGTCAC TGTCCTGGAT GTGAATGACA 
     4321 ACCGGCCCAT CTTTCTGCAG AGCAGCTATG AGGCCAGCGT CCCTGAGGAC ATCCCTGAAG 
     4381 GCCACAGCAT CTTGCAGCTG AAAGCCACGG ACGCAGATGA GGGCGAGTTT GGGCGTGTGT 
     4441 GGTACCGCAT CCTCCATGGT AACCATGGCA ACAACTTCCG GATCCATGTC AGCAATGGGC 
     4501 TCCTGATGCG AGGGCCCCGG CCCCTGGACC GGGAGCGGAA CTCATCCCAC GTGCTGATAG 
     4561 TGGAGGCCTA CAACCACGAC CTGGGCCCCA TGCGGAGCTC CGTCAGGGTG ATTGTGTACG 
     4621 TGGAGGACAT CAACGATGAG GCCCCCGTGT TCACACAGCA GCAGTACAGC CGTCTGGGGC 
     4681 TTCGAGAGAC CGCAGGCATT GGAACGTCAG TCATCGTGGT CCAAGCCACA GACCGAGACT 
     4741 CTGGGGATGG TGGCCTGGTG AACTACCGCA TCCTGTCGGG CGCAGAGGGG AAGTTTGAGA 
     4801 TTGACGAGAG CACAGGGCTT ATCATCACCG TGAATTACCT GGACTACGAG ACCAAGACCA 
     4861 GCTACATGAT GAATGTGTCG GCCACTGACC AGGCCCCGCC CTTCAACCAG GGCTTCTGCA 
     4921 GCGTCTACAT CACTCTGCTC AACGAGCTGG ACGAGGCCGT GCAGTTCTCC AATGCCTCAT 
     4981 ACGAGGCTGC CATCCTGGAG AATCTGGCAC TGGGTACTGA GATTGTGCGG GTCCAGGCCT 
     5041 ACTCCATCGA CAACCTCAAC CAAATCACGT ACCGCTTCAA CGCCTACACC AGCACCCAGG 
     5101 CCAAAGCCCT CTTCAAGATA GACGCCATCA CGGTGAGGGG CTGGGGGCAG GGAGCACCAT 
     5161 TTCTTCCAAT CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     5221 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     5281 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     5341 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     5401 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     5461 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5521 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5581 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5641 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5701 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5761 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5821 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5881 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5941 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     6001 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     6061 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     6121 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     6181 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     6241 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     6301 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     6361 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     6421 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6481 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6541 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6601 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6661 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6721 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6781 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6841 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6901 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6961 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     7021 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     7081 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     7141 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     7201 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     7261 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     7321 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     7381 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     7441 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7501 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7561 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7621 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7681 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7741 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7801 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7861 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7921 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7981 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     8041 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     8101 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     8161 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     8221 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     8281 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8341 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8401 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     8461 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8521 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8581 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8641 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8701 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8761 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8821 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8881 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8941 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     9001 TAACAAAAAT TTAACGCGAA TTTT
//