LOCUS       RC230708                8976 bp    DNA     circular  SYN 
DEFINITION  LRRC16A (Myc-DDK-tagged)-Human leucine rich repeat containing 16A (LRRC16A), transcript variant 2
ACCESSION   NM_001173977
VERSION     NM_001173977.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5133..5224 
                     /label="Myc-DDK"  
     misc_feature    5254..5839 
                     /label="PolyA signal"  
     misc_feature    5311..5330 
                     /label="Restriction Enzyme"  
     misc_feature    6115..6787 
                     /label="ColE1"  
     misc_feature    7217..8011 
                     /label="Kan/Neo"  
     misc_feature    8046..8348 
                     /label="SV40 ori"  
     misc_feature    8434..8911 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5115..5121 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5115 
                     /label="LRRC16A"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACCGAGGAG AGCTCTGACG TTCCCAGGGA GTTGATAGAA AGCATAAAGG 
     1081 ATGTTATTGG CAGAAAGATA AAAATTTCAG TGAAGAAGAA AGTAAAGTTG GAAGTTAAGG 
     1141 GAGACAAAGT TGAAAACAAA GTGCTGGTCC TTACATCATG CCGAGCCTTC CTTGTAACAG 
     1201 CGCGAATCCC CACCAAGCTC GAGTTAACCT TCAGCTACTT GGAGATTCAT GGCGTCGTTT 
     1261 GCAGCAAGTC AGCTCAGATG ATTGTGGAAA CTGAGAAGTG CAGCATTTCC ATGAAGATGG 
     1321 CGTCACCCGA GGACGTGAGT GAGGTGCTGG CTCACATAGG CACCTGCCTG AGGAAGATAT 
     1381 TTCCTGGCCT CTCTCCAGTG AGAATCATGA AAAAAGTCTC CATGGAGCCA TCTGAGCGCC 
     1441 TGGCTAGTCT CCAGGCGCTG TGGGACAGCC AGACCGTGGC TGAGCAGGGC CCCTGTGGTG 
     1501 GATTTTCTCA GATGTATGCC TGTGTTTGTG ACTGGCTTGG ATTTTCATAC AGGGAAGAAG 
     1561 TACAATGGGA TGTGGATACA ATTTATCTTA CCCAAGACAC CAGGGAATTG AATTTACAAG 
     1621 ATTTTAGTCA TCTTGACCAC AGGGACCTAA TACCTATCAT TGCTGCTCTG GAATATAATC 
     1681 AGTGGTTCAC AAAACTGTCC TCTAAGGATC TAAAACTGTC CACTGATGTC TGTGAACAGA 
     1741 TCTTGAGGGT GGTGAGTAGG TCCAATCGAC TGGAAGAATT GGTGTTGGAA AATGCTGGAC 
     1801 TTAGAACAGA TTTTGCACAA AAACTGGCCA GTGCTCTAGC ACATAATCCC AACTCAGGAC 
     1861 TCCACACAAT TAACCTTGCT GGCAACCCAC TGGAGGATAG AGGTGTGTCC TCTTTAAGTA 
     1921 TTCAATTTGC CAAACTCCCA AAGGGATTAA AGCACTTAAA TTTATCTAAA ACCTCATTAT 
     1981 CACCTAAAGG GGTGAACAGC CTTTCTCAGT CACTCAGTGC CAATCCATTG ACCGCCTCTA 
     2041 CCCTTGTCCA CCTCGACCTC TCAGGGAACG TCCTTCGTGG AGATGACCTC TCACACATGT 
     2101 ATAATTTTTT GGCCCAGCCA AATGCCATTG TTCATCTGGA TTTATCCAAT ACAGAATGTT 
     2161 CCCTGGACAT GGTCTGTGGA GCTCTTCTCC GTGGATGCCT TCAATATTTA GCTGTGCTCA 
     2221 ACCTCTCCAG AACTGTCTTC TCTCACCGGA AAGGAAAAGA AGTACCTCCA TCTTTCAAGC 
     2281 AATTTTTTAG TAGTTCTCTG GCTTTGATGC ACATCAACCT TTCAGGCACA AAACTGTCTC 
     2341 CTGAGCCCTT AAAAGCACTG TTATTGGGCC TGGCTTGTAA TCATAACTTG AAAGGGGTTT 
     2401 CTCTGGATCT CAGCAACTGT GAGCTGAGAT CAGGAGGTGC TCAAGTATTA GAAGGTTGCA 
     2461 TTGCTGAAAT ACACAACATC ACCAGCTTAG ACATCTCTGA CAATGGTTTA GAATCTGACC 
     2521 TATCTACCTT AATAGTGTGG CTCAGTAAAA ACAGATCAAT ACAACACCTG GCGTTAGGCA 
     2581 AAAATTTTAA TAATATGAAA TCCAAAAATC TGACACCTGT ATTGGACAAC TTAGTACAGA 
     2641 TGATTCAAGA TGAAGAATCA CCTCTGCAGT CCTTGTCCCT GGCTGACTCG AAACTCAAGA 
     2701 CTGAGGTCAC CATCATCATC AATGCCCTGG GAAGCAACAC CTCCCTGACC AAAGTGGACA 
     2761 TTAGCGGCAA CGGAATGGGG GACATGGGAG CGAAGATGCT GGCCAAAGCA CTGCAGATCA 
     2821 ACACTAAGCT CAGGACTGTA ATATGGGACA AGAACAACAT CACTGCACAA GGCTTTCAGG 
     2881 ATATAGCTGT TGCTATGGAA AAGAACTACA CATTAAGATT TATGCCAATT CCTATGTATG 
     2941 ATGCTTCTCA AGCCCTAAAA ACAAACCCTG AAAAAACAGA AGACGCTCTG CAAAAGATAG 
     3001 AAAACTACCT GCTACGAAAT CACGAGACTA GAAAATACCT TCAAGAGCAG GCCTACCGCC 
     3061 TGCAGCAGGG TATTGTCACC AGCACCACCC AGCAGATGAT TGACAGAATA TGTGTGAAAG 
     3121 TACAAGATCA TCTCAACTCC TTACGAAATT GTGGGGGAGA CGCTATCCAG GAAGATTTAA 
     3181 AATCAGCAGA GCGGCTCATG CGTGATGCTA AGAACTCTAA AACGTTGTTA CCAAATTTAT 
     3241 ACCATGTTGG TGGTGCATCT TGGGCGGGAG CCAGTGGCTT ACTATCCAGT CCAATTCAGG 
     3301 AGACCCTGGA ATCAATGGCT GGAGAAGTTA CAAGAGTAGT AGATGAACAA CTAAAGGCGT 
     3361 TGCTTGAGTC CATGGTTGAT GCTGCTGAGA ATCTTTGTCC CAATGTGATG AAAAAAGCCC 
     3421 ACATTCGACA AGACTTGATT CATGCCAGCA CCGAAAAGAT TTCTATTCCA CGTACCTTTG 
     3481 TTAAAAATGT CCTGTTGGAG CAGTCTGGAA TTGATATCCT TAACAAAATT AGTGAAGTAA 
     3541 AATTGACAGT GGCCTCGTTC CTGTCTGATA GAATTGTGGA TGAAATCCTG GATGCACTCT 
     3601 CACATTGCCA TCATAAACTG GCTGACCATT TCAGCAGACG TGGCAAGACC CTTCCTCAAC 
     3661 AAGAATCCTT AGAGATCGAG CTGGCTGAGG AGAAGCCAGT TAAACGTTCC ATCATCACAG 
     3721 TGGAGGAGCT AACAGAGATA GAGCGTTTGG AAGATCTGGA TACCTGTATG ATGACCCCTA 
     3781 AATCCAAAAG GAAGAGTATC CATAGCCGAA TGCTGCGGCC TGTTTCTAGG GCTTTTGAAA 
     3841 TGGAGTTTGA TCTAGATAAA GCGCTGGAAG AGGTACCAAT TCACATCGAA GACCCGCCCT 
     3901 TCCCATCCCT CAGACAGGAG AAGCGGAGCT CGGGATTTAT CTCTGAGTTG CCCTCTGAAG 
     3961 AGGGGAAGAA GCTGGAACAC TTTACCAAGT TAAGGCCAAA AAGGAATAAG AAGCAGCAAC 
     4021 CCACCCAAGC AGCGGTCTGT GCTGCCAACA TAGTCTCACA AGATGGTGAA CAGAATGGTC 
     4081 TCATGGGGAG AGTGGATGAA GGTGTAGATG AATTTTTTAC CAAGAAGGTG ACCAAAATGG 
     4141 ATTCCAAGAA ATGGTCAACA AGAGGCTCAG AGTCCCATGA GCTTAATGAA GGAGGAGATG 
     4201 AAAAGAAAAA GCGAGATTCT CGGAAAAGTA GTGGCTTTCT CAATTTAATC AAATCCCGGT 
     4261 CCAAATCCGA GCGACCACCA ACGATCTTGA TGACAGAAGA ACCCTCCTCA CCAAAAGGGG 
     4321 CAGTCAGAAG TCCACCTGTG GACTGTCCCA GGAAGGACAC AAAGGCCGCC GAGCACAATG 
     4381 GCAATTCTGA ACGGATAGAG GAGATAAAAA CACCTGACTC CTTTGAAGAG AGTCAAGGGG 
     4441 AAGAAATAGG GAAGGTGGAA CGGAGTGACA GCAAGAGCAG CCCACAGGCA GGGCGGAGGT 
     4501 ATGGGGTCCA GGTGATGGGC AGTGGTCTGC TGGCAGAGAT GAAAGCCAAG CAAGAGAAGA 
     4561 GAGCTGCGTG TGCGCAGAAG AAGCTTGGGA ATGATGCCGT ATCCCAGGAT TCTTCCAGCC 
     4621 CAGCTTTGAG CGGCGTAGAA CGGTCGGATG GAGGTGGGGC AGGTCTTCCA GAGAACCGCT 
     4681 TTGGTTTGGG AACACCAGAA AAGAATACCA AAGCAGAACC CAAAGCGGAA GCAGGCTCCA 
     4741 GGTCTCGGAG CTCATCCAGC ACACCTACGA GCCCGAAGCC CCTCCTGCAG TCCCCCAAAC 
     4801 CCAGTCTGGC AGCACGGCCC GTCATCCCGC AGAAACCAAG AACCGCCTCA CGGCCTGATG 
     4861 ACATTCCAGA CTCTCCATCT AGCCCGAAAG TTGCCCTTCT TCCACCTGTC CTGAAAAAAG 
     4921 TTCCTTCAGA CAAAGAGAGA GATGGCCAGA GTAGCCCCCA GCCCAGCCCC AGGACATTTT 
     4981 CACAGGAAGT TTCAAGGAGA AGCTGGGGCC AGCAGGCCCA GGAGTATCAA GAACAAAAGC 
     5041 AACGGTCCTC CAGTAAAGAT GGCCATCAAG GCAGCAAATC TAATGACTCC GGGGAAGAAG 
     5101 CAGAAAAAGA GTTTATTTTT GTGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     5161 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     5221 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     5281 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     5341 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     5401 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5461 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5521 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5581 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5641 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5701 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5761 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5821 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5881 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5941 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     6001 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     6061 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     6121 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     6181 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     6241 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     6301 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     6361 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6421 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6481 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6541 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6601 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6661 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6721 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6781 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6841 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6901 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6961 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     7021 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     7081 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     7141 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     7201 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     7261 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     7321 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     7381 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7441 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7501 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7561 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7621 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7681 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7741 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7801 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7861 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7921 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7981 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     8041 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     8101 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     8161 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     8221 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8281 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8341 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     8401 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8461 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8521 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8581 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8641 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8701 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8761 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8821 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8881 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8941 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//