LOCUS       RC230705                8904 bp    DNA     circular  SYN 
DEFINITION  IGSF1 (Myc-DDK-tagged)-Human immunoglobulin superfamily, member 1 (IGSF1), transcript variant 3
ACCESSION   NM_001170961
VERSION     NM_001170961.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5061..5152 
                     /label="Myc-DDK"  
     misc_feature    5182..5767 
                     /label="PolyA signal"  
     misc_feature    5239..5258 
                     /label="Restriction Enzyme"  
     misc_feature    6043..6715 
                     /label="ColE1"  
     misc_feature    7145..7939 
                     /label="Kan/Neo"  
     misc_feature    7974..8276 
                     /label="SV40 ori"  
     misc_feature    8362..8839 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5043..5049 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5043 
                     /label="IGSF1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACCCTGGAC AGACCAGGGG AGGGGGCCAC CATGCTGAAG ACATTCACTG 
     1081 TTTTGCTCTT TTGCATTCGG ATGAGTCTGG GTATGACATC GATAGTGATG GACCCTCAAC 
     1141 CGGAGTTGTG GATAGAGTCC AACTACCCCC AGGCCCCTTG GGAGAACATC ACGCTTTGGT 
     1201 GCCGAAGCCC CTCTCGGATA TCAAGCAAGT TCCTGCTGCT GAAGGATAAG ACACAGATGA 
     1261 CCTGGATCCG CCCTTCCCAC AAGACCTTCC AAGTTTCATT CCTTATAGGT GCCCTTACTG 
     1321 AGTCCAATGC AGGTCTTTAC CGGTGCTGCT ACTGGAAGGA GACAGGCTGG TCAAAGCCCA 
     1381 GTAAAGTTCT AGAGTTGGAG GCACCAGGCC AACTGCCCAA GCCCATCTTC TGGATTCAGG 
     1441 CTGAGACCCC CGCTCTTCCT GGGTGTAATG TTAACATCCT CTGCCATGGC TGGCTGCAGG 
     1501 ATTTGGTATT CATGCTGTTT AAAGAGGGAT ATGCAGAGCC TGTGGATTAC CAAGTCCCAA 
     1561 CTGGGACAAT GGCCATATTC TCCATTGACA ACCTGACACC TGAGGATGAA GGGGTTTACA 
     1621 TCTGCCGCAC TCATATCCAG ATGCTCCCCA CCCTGTGGTC AGAGCCCAGC AACCCCCTGA 
     1681 AGCTGGTTGT AGCAGGACTC TACCCCAAAC CAACTTTGAC AGCCCATCCT GGGCCCATCA 
     1741 TGGCACCTGG AGAAAGCCTG AATCTCAGGT GCCAAGGGCC AATCTATGGA ATGACCTTTG 
     1801 CTCTAATGAG GGTTGAAGAC TTGGAGAAGT CCTTTTACCA CAAGAAGACA ATAAAAAATG 
     1861 AGGCAAATTT CTTCTTCCAG TCTTTGAAGA TCCAAGATAC TGGACATTAC CTCTGTTTTT 
     1921 ACTATGACGC ATCATATAGA GGTTCACTCC TTAGTGATGT CCTGAAAATC TGGGTGACTG 
     1981 ACACTTTCCC CAAGACCTGG CTACTTGCTC GGCCCAGTGC TGTGGTCCAA ATGGGTCAGA 
     2041 ATGTGAGCCT ACGGTGTCGA GGACCAGTGG ATGGAGTGGG TCTTGCACTC TATAAGAAAG 
     2101 GAGAAGACAA ACCACTTCAA TTTTTGGATG CCACCAGCAT CGATGACAAC ACATCATTCT 
     2161 TCCTCAACAA TGTAACCTAC AGTGATACTG GCATCTATAG CTGCCACTAT CTTCTCACCT 
     2221 GGAAGACCTC CATTAGGATG CCATCACACA ACACTGTGGA GCTTATGGTT GTAGATAAGC 
     2281 CCCCCAAACC CTCCCTGTCA GCTTGGCCAA GCACTGTGTT CAAGCTAGGA AAGGCCATCA 
     2341 CCCTTCAGTG CCGAGTATCT CATCCAGTAC TGGAATTTTC TCTGGAATGG GAAGAAAGAG 
     2401 AAACATTCCA AAAATTCTCA GTAAACGGAG ACTTCATCAT CAGTAATGTT GACGGGAAAG 
     2461 GCACAGGGAC CTACAGTTGC AGCTATCGCG TAGAGACACA TCCTAACATC TGGTCACATC 
     2521 GCAGTGAGCC CCTGAAGCTG ATGGGGCCAG CAGGCTATCT CACCTGGAAT TACGTTCTGA 
     2581 ATGAAGCTAT CAGGTTGTCT CTAATCATGC AGCTTGTTGC CTTGCTGTTG GTAGTGCTGT 
     2641 GGATAAGGTG GAAGTGTCGG AGACTCAGAA TCAGAGAAGC CTGGTTGCTG GGAACAGCTC 
     2701 AAGGGGTCAC CATGCTCTTC ATAGTCACGG CCCTTCTCTG CTGTGCAATT TCTTTTGCAG 
     2761 GACTGTGCAA TGGGGTATTG ATAGAAGAGA CTGAAATAGT CATGCCAACC CCTAAGCCTG 
     2821 AGCTGTGGGC AGAGACCAAC TTTCCTCTGG CCCCGTGGAA GAACTTAACC CTCTGGTGCA 
     2881 GAAGCCCTTC TGGCTCAACT AAGGAGTTTG TGTTGCTGAA GGATGGGACC GGGTGGATCG 
     2941 CCACTCGCCC GGCCTCAGAG CAGGTCCGGG CTGCCTTCCC CCTTGGCGCC CTGACCCAGA 
     3001 GCCACACCGG GAGCTACCAC TGCCATTCAT GGGAGGAGAT GGCTGTATCG GAGCCCAGTG 
     3061 AGGCACTTGA GCTGGTGGGG ACAGACATCC TCCCCAAACC TGTCATTTCT GCTTCCCCCA 
     3121 CAATCCGGGG CCAGGAACTA CAACTCCGGT GCAAAGGATG GCTGGCAGGC ATGGGGTTTG 
     3181 CTCTGTATAA GGAGGGAGAG CAAGAACCTG TCCAGCAACT TGGTGCTGTT GGAAGAGAAG 
     3241 CCTTCTTTAC AATCCAGAGA ATGGAGGATA AAGACGAAGG CAATTACAGC TGCCGCACTC 
     3301 ACACTGAAAA ACGCCCCTTC AAGTGGTCTG AGCCCAGTGA GCCGCTGGAG CTTGTCATAA 
     3361 AAGAAATGTA CCCTAAGCCC TTCTTCAAGA CATGGGCCAG CCCTGTGGTC ACCCCTGGTG 
     3421 CCCGAGTGAC TTTCAATTGC TCCACCCCCC ACCAGCATAT GAGCTTTATT CTTTACAAAG 
     3481 ATGGAAGTGA AATAGCATCC AGTGACAGGT CCTGGGCAAG TCCGGGGGCC AGTGCAGCTC 
     3541 ACTTTCTAAT CATTTCGGTG GGCATTGGTG ATGGAGGGAA TTACAGCTGC CGATATTATG 
     3601 ACTTTTCTAT CTGGTCTGAG CCCAGCGACC CTGTGGAGCT CGTGGTGACA GAATTCTACC 
     3661 CCAAACCCAC TCTCCTGGCA CAGCCAGGTC CTGTGGTGTT TCCTGGGAAG AGTGTGATCC 
     3721 TGCGCTGCCA AGGGACTTTC CAGGGCATGA GGTTCGCCCT CTTGCAGGAG GGAGCCCATG 
     3781 TTCCCTTACA GTTTCGGAGT GTCTCAGGGA ACTCAGCTGA CTTCCTTCTC CACACTGTTG 
     3841 GAGCAGAGGA CTCTGGGAAC TATAGCTGTA TCTACTATGA GACAACCATG TCAAACAGGG 
     3901 GGTCATATCT CAGTATGCCC CTTATGATCT GGGTGACTGA CACATTCCCT AAGCCATGGT 
     3961 TGTTTGCTGA GCCCAGTTCT GTGGTTCCCA TGGGGCAGAA TGTTACTCTC TGGTGCCGAG 
     4021 GGCCGGTCCA TGGAGTAGGA TACATTCTGC ACAAAGAAGG AGAAGCCACT TCAATGCAGC 
     4081 TCTGGGGATC CACCAGTAAT GACGGGGCAT TCCCCATCAC CAATATATCT GGTACTAGCA 
     4141 TGGGGCGTTA CAGCTGCTGC TACCACCCTG ACTGGACCAG TTCTATCAAG ATACAACCTA 
     4201 GCAACACCCT GGAACTCCTA GTCACAGGCT TACTCCCCAA ACCCAGCCTA TTAGCCCAGC 
     4261 CTGGTCCCAT GGTGGCCCCT GGCGAAAATA TGACTCTTCA GTGTCAAGGG GAACTGCCAG 
     4321 ACTCAACATT TGTCCTGTTG AAGGAGGGGG CTCAGGAGCC TTTAGAGCAA CAGAGGCCAA 
     4381 GTGGGTACAG GGCTGACTTC TGGATGCCAG CAGTGAGAGG TGAAGACTCT GGGATCTATA 
     4441 GCTGTGTTTA TTATTTGGAC TCTACTCCCT TTGCAGCTTC AAATCACAGT GACTCCCTGG 
     4501 AGATCTGGGT GACTGATAAG CCCCCTAAAC CCTCTCTGTC AGCCTGGCCC AGCACCATGT 
     4561 TCAAGTTAGG GAAGGACATC ACCCTTCAGT GCCGAGGACC CCTGCCAGGT GTTGAATTTG 
     4621 TCCTAGAACA TGATGGAGAA GAAGCACCTC AGCAGTTTTC AGAGGATGGA GACTTTGTCA 
     4681 TCAACAACGT AGAAGGAAAA GGCATTGGAA ACTACAGCTG CAGCTACCGC CTCCAGGCCT 
     4741 ACCCTGATAT CTGGTCAGAG CCTAGTGATC CCCTGGAGCT GGTGGGGGCA GCAGGGCCTG 
     4801 TTGCTCAGGA GTGCACTGTA GGGAACATTG TCCGAAGTAG CCTAATCGTG GTGGTTGTTG 
     4861 TAGCCTTGGG GGTAGTGCTA GCCATAGAGT GGAAGAAGTG GCCTCGACTG CGAACCAGAG 
     4921 GCTCAGAGAC AGACGGAAGA GACCAGACCA TTGCCCTTGA AGAGTGTAAC CAAGAAGGAG 
     4981 AACCAGGCAC CCCTGCCAAT TCTCCTTCAT CAACCTCTCA GAGAATCTCT GTGGAACTGC 
     5041 CCGTTCCAAT AACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     5101 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     5161 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     5221 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     5281 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     5341 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5401 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5461 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5521 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5581 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5641 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5701 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5761 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5821 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5881 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5941 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     6001 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     6061 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     6121 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     6181 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     6241 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     6301 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6361 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6421 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6481 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6541 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6601 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6661 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6721 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6781 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6841 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6901 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6961 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     7021 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     7081 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     7141 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     7201 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     7261 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     7321 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7381 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7441 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7501 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7561 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7621 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7681 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7741 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7801 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7861 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7921 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7981 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     8041 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     8101 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     8161 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8221 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8281 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     8341 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8401 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8461 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8521 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8581 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8641 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8701 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8761 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8821 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8881 TAACAAAAAT TTAACGCGAA TTTT
//